Enhanced CT-Based Intratumoral and Peritumoral Radiomics Nomograms Predict High-Grade Patterns of Invasive Lung Adenocarcinoma.

RATIONALE AND OBJECTIVES: Extraction of intratumoral and peritumoral radiomics features combined with clinical factors to establish nomograms to predict high-grade patterns (micropapillary and solid) of invasive adenocarcinoma of the lung (IAC). MATERIALS AND METHODS: A retrospective study was conducted on 463 patients with pathologically confirmed IAC. Patients were randomized in a 7:3 ratio into a training cohort (n = 324) and a testing cohort (n = 139). A total of 2154 CT-based radiomic features were extracted from each of the four regions: gross tumor volume (GTV) and gross peritumoral tumor volume (GPTV3, GPTV6, GPTV9) containing peri-tumor regions of 3 mm, 6 mm, and 9 mm. A radiomics nomogram was constructed based on the optimal radiomics model and clinically independent predictors. RESULTS: The GPTV3 radiomics model showed better predictive performance in the testing group compared to the GTV (0.840), GPTV6 (0.843), and GPTV9 (0.734) models, with an AUC value of 0.889 in the testing group. In the clinical model, tumor density and the presence of a spiculation sign were identified as independent predictors. The nomogram, which combined these independent predictors with the GPTV3-Radscore, proved to be clinically useful. CONCLUSION: The GPTV3 radiomics model was superior to the GTV, GPTV6, and GPTV9 radiomics models in predicting high-grade patterns (HGP) of IAC. In addition, nomograms based on GPTV3 radiomics features and clinically independent predictors can further improve the prediction efficiency.

Triglyceride glucose index is associated with functional coronary artery stenosis in hypertensive patients.

Background: The triglyceride glucose (TyG) index is an effective method for determining insulin resistance (IR). Limited research has explored the connection between the TyG index and functionally significant stenosis in hypertensive patients. Furthermore, the connections between the TyG index, fat attenuation index (FAI) and atherosclerotic plaque characteristics are also worth exploring. Methods: The study screened 1622 hypertensive participants without coronary artery disease history who underwent coronary computed tomography angiography. The TyG index was calculated as ln (fasting glucose [mg/dL] * fasting TG [mg/dL]/2). Adverse plaque characteristics (HRPCs), high-risk plaques (HRPs), FAI, and CT-derived fractional flow reserve (FFRCT) were analyzed and measured for all patients. Functionally significant stenosis causing ischemia is defined as FFRCT ≤ 0.80. Two patient groups were created based on the FFRCT: the FFRCT < 0.80 group and the FFRCT > 0.80 group. In hypertensive patients, the association between the TyG index and FFRCT was examined applying a logistic regression model. Results: The TyG index was higher for people with FFRCT ≤ 0.80 contrast to those with FFRCT > 0.80. After controlling for additional confounding factors, the logistic regression model revealed a clear connection between the TyG index and FFRCT ≤ 0.80 (OR = 1.718, 95% CI 1.097-2.690, p = 0.018). The restricted cubic spline analysis displayed a nonlinear connection between the TyG index and FFRCT ≤ 0.80 (p for nonlinear = 0.001). The TyG index increased the fraction of individuals with HRPs and HRPCs, FAI raised, and FFRCT decreased (p < 0.05). The multivariate linear regression analysis illustrated a powerfulcorrelation between high TyG index levels and FAI, FFRCT, positive remodeling (PR), and low-attenuation plaque (LAPs) (standardized regression coefficients: 0.029 [p = 0.007], -0.051 [p < 0.001], 0.029 [p = 0.027], and 0.026 [p = 0.046], separately). Conclusion: In hypertensive patients, the TyG index showed an excellent association with a risk of FFRCT ≤ 0.80. Additionally, the TyG index was also linked to FAI, FFRCT, PR, and LAPs.

Ruptured Anterior Communicating Artery Aneurysms Concomitant with Small Intracranial Hematoma: Evacuating Hematoma or Not?

Objective: To identify whether intracranial hematoma (ICH) evacuation improves the prognosis of patients with ruptured anterior communicating artery (AcomA) aneurysms concomitant with small ICHs (≥10 mL and <25 mL). Methods: Data on patients diagnosed with small ICHs secondary to ruptured AcomA aneurysms who underwent surgery in our department between January 2010 and February 2018 was retrospectively analyzed. The patients were divided into two groups based on whether the hematoma was evacuated. The modified Rankin Scale (mRS) was used to assess prognosis six months after onset. Results: The study recruited 58 patients, 19 of whom underwent aneurysm clipping and ICH evacuation. While 33 patients underwent aneurysm clipping, 6 patients underwent coiling embolism without ICH evacuation. The average ICH volume was 15.27±4.07 mL. In the hematoma-evacuated group, 13 (68.4%) patients had unfavorable outcomes (mRS scores of 4 to 6). In the non-evacuated hematoma group, 13 (33.3%) patients had unfavorable outcomes (P = 0.001), postoperative infarction occurred in 11 (57.9%) patients in the hematoma evacuation group and 9 (23.1%) patients in the other group (P = 0.009). Conclusion: ICH evacuation was associated with unfavorable outcomes and postoperative infarction in ruptured AcomA aneurysms with concomitant small hematomas (<25 mL). Aneurysm clipping or coiling without ICH evacuation may be a safe and effective choice; however, further investigation is needed.

Fluorescent double network ionogels with fast self-healability and high resilience for reliable human motion detection.

Fascinating properties are displayed by high-performance ionogel-based flexible strain sensors, thereby gaining increasing attention in various applications ranging from human motion monitoring to soft robotics. However, the integration of excellent properties such as optical and mechanical properties and satisfactory sensing performance for one ionogel sensor is still a challenge. In particular, fatigue-resistant and self-healing properties are essential to continuous sensing. Herein, we design a flexible ion-conductive sensor based on a multifunctional ionogel with a double network using polyacrylamide, amino-modified agarose, 1,3,5-benzenetricarboxaldehyde and 1-ethyl-3-methylimidazolium chloride. The ionogel exhibits comprehensive properties including high transparency (>95%), nonflammability, strong adhesion and good temperature tolerance (about -96 to 260 °C), especially adaptive for extreme conditions. The dynamic imine bonds and abundant hydrogen bonds endow the ionogel with excellent self-healing capability, to realize rapid self-repair within minutes, as well as good mechanical properties and ductility to dissipate input energy and realize high resilience. Notably, unexpected fluorescence has been observed for the ionogel because of the gelation-induced emission phenomenon. Flexible strain sensors prepared directly from ionogels can sensitively monitor and differentiate various human motions, exhibiting a fast response time (38 ms), high sensitivity (gauge factor = 3.13 at 800% strain), good durability (>1000 cycles) and excellent stability over a wide temperature range (-30 to 80 °C). Therefore, the prepared ionogel as a high-performance flexible strain sensor in this study shows tremendous potential in wearable devices and soft ionotronics.

Mitigative effects of dehydrodiisoeugenol on enteritis and co-occurring dysmotility in murine model.

The actions and mechanisms of dehydrodiisoeugenol (DEH) on releasing clinical symptoms such as diarrhea caused by inflammatory bowel diseases or colorectal cancer is still unclear. The main purpose is to reveal the mechanism and describe the impacts of DEH on enteritis and accompanying intestinal dysmotility in murine model. The animal model of diarrhea was established through being given acetic acid by intracolonic instillation and restraint stress and the weight of the diarrhea mouse, diarrhea index (the product of stool rate and stool grade) evaluation and then, myeloperoxidase (MPO) activity were determined after administrated with DEH. Meanwhile, the expression of myosin light chain kinase (MLCK) was research by WB method. Moreover, the isolated jejunal segment (IJS) of rats was separated from the intact jejunum and the contractility was measured through BL-420F physiological recording system. DEH could significantly inhibit the intestinal transit in normal mice or diarrhea-predominated mice and reduce the diarrhea index and the level of MPO in mice. DEH concentration-dependently inhibited motility of IJS in different states. DEH significantly markedly ameliorated the histopathology condition and reduce the MLCK expression in acetic acid induced diarrhea mice. DEH simultaneously improved enteritis and co-occurring dysmotility in diarrhea mice characterized by reducing the contractility and MLCK contents in acetic acid induced diarrhea mice.

Establishment and Validation of an Integrated Model to Predict Postoperative Recurrence in Patients With Atypical Meningioma.

BACKGROUND: This study aims to establish an integrated model based on clinical, laboratory, radiological, and pathological factors to predict the postoperative recurrence of atypical meningioma (AM). MATERIALS AND METHODS: A retrospective study of 183 patients with AM was conducted. Patients were randomly divided into a training cohort (n = 128) and an external validation cohort (n = 55). Univariable and multivariable Cox regression analyses, the least absolute shrinkage and selection operator (LASSO) regression analysis, time-dependent receiver operating characteristic (ROC) curve analysis, and evaluation of clinical usage were used to select variables for the final nomogram model. RESULTS: After multivariable Cox analysis, serum fibrinogen >2.95 g/L (hazard ratio (HR), 2.43; 95% confidence interval (CI), 1.05-5.63; p = 0.039), tumor located in skull base (HR, 6.59; 95% CI, 2.46-17.68; p < 0.001), Simpson grades III-IV (HR, 2.73; 95% CI, 1.01-7.34; p = 0.047), tumor diameter >4.91 cm (HR, 7.10; 95% CI, 2.52-19.95; p < 0.001), and mitotic level ≥4/high power field (HR, 2.80; 95% CI, 1.16-6.74; p = 0.021) were independently associated with AM recurrence. Mitotic level was excluded after LASSO analysis, and it did not improve the predictive performance and clinical usage of the model. Therefore, the other four factors were integrated into the nomogram model, which showed good discrimination abilities in training cohort (C-index, 0.822; 95% CI, 0.759-0.885) and validation cohort (C-index, 0.817; 95% CI, 0.716-0.918) and good match between the predicted and observed probability of recurrence-free survival. CONCLUSION: Our study established an integrated model to predict the postoperative recurrence of AM.

Serum Inflammatory Biomarkers Contribute to the Prognosis Prediction in High-Grade Glioma.

BACKGROUND: To evaluate the prognostic value of serum inflammatory biomarkers and develop a risk stratification model for high-grade glioma (HGG) patients based on clinical, laboratory, radiological, and pathological factors. MATERIALS AND METHODS: A retrospective study of 199 patients with HGG was conducted. Patients were divided into a training cohort (n = 120) and a validation cohort (n = 79). The effects of potential associated factors on the overall survival (OS) time were investigated and the benefits of serum inflammatory biomarkers in improving predictive performance was assessed. Univariable and multivariable Cox regression analyses, the least absolute shrinkage and selection operator (LASSO) regression analysis, and support vector machines (SVM) were used to select variables for the final nomogram model. RESULTS: After multivariable Cox, LASSO, and SVM analysis, in addition to 3 other clinico-pathologic factors, platelet-to-lymphocyte ratio (PLR) >144.4 (hazard ratio [HR], 2.05; 95% confidence interval [CI], 1.25-3.38; P = 0.005) were left for constructing the predictive model. The model with PLR exhibited a better predictive performance than that without them in both cohorts. The nomogram based on the model showed an excellent ability of discrimination in the entire cohort (C-index, 0.747; 95%CI, 0.706-0.788). The calibration curves showed good consistency between the predicted and observed survival probability. CONCLUSION: Our study confirmed the prognostic value of serum inflammatory biomarkers including PLR and established a comprehensive scoring system for the OS prediction in HGG patients.

Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells.

Meningioma (MEN) is a common central nervous system disease. Accumulating evidence indicated that long non-coding RNA maternally expressed gene 3 (MEG3) participated in the progression of MEN. However, the potential mechanisms of MEG3 in altering the aggressive phenotypes of MEN need further exploration. Levels of MEG3, microRNA (miR)-29c, and A-kinase anchor protein 12 (AKAP12) were determined using quantitative real-time Polymerase Chain Reaction (qRT-PCR) assay. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to verify the relationship between miR-29c and MEG3 or AKAP12. The protein level of AKAP12 was detected by western blot. Moreover, cell-cycle arrest, migration, invasion, and proliferation were assessed by flow cytometry, wound healing, transwell assays, and CCK-8 assay, respectively. Levels of MEG3 and AKAP12 were downregulated, while miR-29c was effectively increased in MEN tissues and cell line. Mechanically, MEG3 was a sponge of miR-29c to regulate the expression of AKAP12. Functionally, increase of MEG3 diminished cell-cycle, migration, invasion, and proliferation in MEN cells, and reintroduction of miR-29c could eliminate these effects. In addition, AKAP12 depletion overturned the inhibitory effects of miR-29c absence on cell-cycle, migration, invasion, and proliferation in vitro. Also, AKAP12 was co-regulated by MEG3/miR-29c axis. MEG3 mediated the aggressive behaviors of MEN cells via miR-29c/AKAP12 axis, supporting that MEG3 served as a promising biomarker for the diagnosis and treatment of human MEN.

Predictive factors of postoperative infection-related complications in adult patients with cerebral cavernous malformations.

Postoperative infection is an important factor contributing to poor prognosis after surgical treatment of cerebral cavernous malformations (CCM). However, the predictive factors of postoperative infection-related complications in adult patients with CCM have still not been well established. To identify possible predictive factors of postoperative infection after CCM surgery, we retrospectively evaluated the data of CCM patients who were enrolled into our prospective registry database. The relationship between preoperative characteristics of patients and postoperative infection-related complications was analyzed. A total of 167 CCM patients were included in this study. The average age was 39.69 ± 15.27 years old, and 21 of them had postoperative infection. For patients with postoperative infection, the Glasgow Coma Scale (GCS), Modified Rankin Scale (mRS), white blood cell (WBC) count, and neutrophil (NEU) count were all significantly higher than those of the group without infection. Our preliminary results showed that NEU count might have significant predictive value of intracranial infection, and GCS, mRS and CCM presenting with hemorrhage were all factors significantly related to postoperative pneumonia. Preoperative GCS, mRS and CCM presenting with hemorrhage might be used as predictive factors for postoperative pneumonia after CCM surgery, while preoperative NEU count can be used as an important predictive factor for postoperative intracranial infection after CCM surgery. Further large-scale studies are still needed to confirm this finding.

Heptanuclear CoII5CoIII2 Cluster as Efficient Water Oxidation Catalyst.

Inspired by the transition-metal-oxo cubical Mn4CaO5 in photosystem II, we herein report a disc-like heptanuclear mixed-valent cobalt cluster, [CoII5CoIII2(mdea)4(N3)2(CH3CN)6(OH)2(H2O)2·4ClO4] (1, H2mdea = N-methyldiethanolamine), for photocatalytic oxygen evolution. The topology of the Co7 core resembles a small piece of cobaltate protected by terminal H2O, N3-, CH3CN, and multidentate N-methyldiethanolamine at the periphery. Under the optimal photocatalytic conditions, 1 exhibits water oxidation activity with a turnover number (TON) of 210 and a turnover frequency (TOFinitial) of 0.23 s-1. Importantly, electrospray mass spectrometry (ESI-MS) was used to not only identify the possible main active species in the water oxidation reaction but also monitor the evolutions of oxidation states of cobalt during the photocatalytic reactions. These results shed light on the design concept of new water oxidation catalysts and mechanism-related issues such as the key active intermediate and oxidation state evolution in the oxygen evolution process. The magnetic properties of 1 were also discussed in detail.

Kagóme Cobalt(II)-Organic Layers as Robust Scaffolds for Highly Efficient Photocatalytic Oxygen Evolution.

Two Kagóme cobalt(II)-organic layers of [Co3 (µ3 -OH)2 (bdc)2 ]n (1) and [Co3 (µ3 -OH)2 (chdc)2 ]n (2) (bdc=o-benzenedicarboxylate and chdc=1,2-cyclohexanedicarboxylate) that bear bridging OH(-) ligands were explored as water oxidation catalysts (WOCs) for photocatalytic O2 production. The activities of 1 and 2 towards H2 O oxidation were assessed by monitoring the in situ O2 concentration versus time in the reaction medium by utilizing a Clark-type oxygen electrode under photochemical conditions. The oxygen evolution rate (RO2 ) was 24.3 µmol s(-1) g(-1) for 1 and 48.8 µmol s(-1) g(-1) for 2 at pH 8.0. Photocatalytic reaction studies show that 1 and 2 exhibit enhanced activities toward the oxidation of water compared to commercial nanosized Co3 O4 . In scaled-up photoreactions, the pH value of the reaction medium decreased from 8.0 to around 7.0 after 20 min and the O2 production ceased. Based on the amounts of the sacrificial oxidant (K2 S2 O8 ) used, the yield of O2 produced is 49.6 % for 2 and 29.8 % for 1. However, the catalyst can be recycled without a significant loss of catalytic activity. Spectroscopic studies suggest that the structure and composition of recycled 1 and 2 are maintained. In isotope-labeling H2 (18) O (97 % enriched) experiments, the distribution of (16) O(16) O/(16) O(18) O/(18) O(18) O detected was 0:7.55:92.45, which is comparable to the theoretical values of 0.09:5.82:94.09. This work not only provides new catalysts that resemble ligand-protected cobalt oxide materials but also establishes the significance of the existence of OH(-) (or H2 O) binding sites at the metal center in WOCs.

Hierarchical Assembly of a {Mn(II)15Mn(III)4} Brucite Disc: Step-by-Step Formation and Ferrimagnetism.

In search of functional molecular materials and the study of their formation mechanism, we report the elucidation of a hierarchical step-by-step formation from monomer (Mn) to heptamer (Mn7) to nonadecamer (Mn19) satisfying the relation 1 + Σn6n, where n is the ring number of the Brucite structure using high-resolution electrospray ionization mass spectrometry (HRESI-MS). Three intermediate clusters, Mn10, Mn12, and Mn14, were identified. Furthermore, the Mn19 disc remains intact when dissolved in acetonitrile with a well-resolved general formula of [Mn19(L)x(OH)y(N3)36-x-y](2+) (x = 18, 17, 16; y = 8, 7, 6; HL = 1-(hydroxymethyl)-3,5-dimethylpyrazole) indicating progressive exchange of N3(-) for OH(-). The high symmetry (R-3) Mn19 crystal structure consists of a well-ordered discotic motif where the peripheral organic ligands form a double calix housing the anions and solvent molecules. From the formula and valence bond sums, the charge state is mixed-valent, [Mn(II)15Mn(III)4]. Its magnetic properties and electrochemistry have been studied. It behaves as a ferrimagnet below 40 K and has a coercive field of 2.7 kOe at 1.8 K, which can be possible by either weak exchange between clusters through the anions and solvents or through dipolar interaction through space as confirmed by the lack of ordering in frozen CH3CN. The moment of nearly 50 NµB suggests Mn(II)-Mn(II) and Mn(III)-Mn(III) are ferromagnetically coupled while Mn(II)-Mn(III) is antiferromagnetic which is likely if the Mn(III) are centrally placed in the cluster. This compound displays the rare occurrence of magnetic ordering from nonconnected high-spin molecules.

Preparation and functions of hybrid membranes with rings of Ag NPs anchored at the edges of highly ordered honeycomb-patterned pores.

We report a new, simple strategy to apply honeycomb films for the patterning of colloidal particles. By combination of a "bottom-up" breath figure method and the electrochemical properties of the honeycomb films of ferrocenyl-based oligomers, highly ordered hybrid membranes coated with ring-like patterning of 0D- and 1D-Ag nanoparticles (NPs) have been fabricated. One interesting phenomenon is that the nucleation and adsorption of Ag dots occurred preferentially at the edges of the micropores. The hybrid membranes exhibited richly electrochemical activities towards reduction of iodate and enhanced effectively catalytic reduction of organic dyes. We believe that this method can be used to decorate and/or assemble functional metal NPs such as Au, Pd, and Cu on honeycomb-patterned materials for the further applications of photonics, sensors, and catalysis.