RESUMEN
Oxyhemoglobin (HbO2) coexisting with equimolar NADH retards autoxidation and oxidant-induced metHb formation based on the pseudocatalase (CAT) and pseudosuperoxide dismutase (SOD) activities. In this work, we compared the effects of NADH with those of NADPH and estimated the binding site of NAD(P)H to HbO2 to elucidate the antioxidative mechanisms. The results clarified that pseudo-CAT and pseudo-SOD activities of HbO2 coexisting with NADPH were similar to activities obtained with NADH. Prompt MetHb formation (<40 min) facilitated by oxidants (H2O2, NO, and NaNO2) was hindered by NADPH. These effects were similar to those of NADH. However, we found that NADPH is thermally unstable compared to NADH and that NADPH cannot sustain antioxidative effects for a long period of autoxidation to metHb such as 24 h. Lineweaver-Burk plots clarified that the Michaelis constants of these pseudoenzymatic activities are in the millimolar range. Addition of inositol hexaphosphate (IHP) and 2,3-diphosphoglycerate (DPG), which are known to bind not only with deoxyHb but also weakly with HbO2, showed competitive inhibition of pseudoenzymatic activities. These results suggest that the binding site of NADH and NADPH on HbO2 is the same as those of IHP and DPG. 31P nuclear magnetic resonance definitively showed 1:1 stoichiometric binding of NADH to HbO2. High-performance liquid chromatography analysis showed that NADH preferentially inhibited autoxidation of α-subunit heme. Docking simulations also predicted that the binding site of relaxed-state HbO2 with NAD(P)H is the same as those with IHP and DPG. Collectively, the pseudoenzymatic activities of HbO2 coexisting with NAD(P)H are induced by the 1:1 stoichiometric binding of NAD(P)H to HbO2.
Asunto(s)
Metahemoglobina/biosíntesis , NADP/metabolismo , Oxihemoglobinas/metabolismo , Antioxidantes/metabolismo , Sitios de Unión/genética , Catalasa/metabolismo , Hemoglobinas , Peróxido de Hidrógeno , Metahemoglobina/metabolismo , NAD/metabolismo , Oxidantes , Oxidación-Reducción , Superóxido Dismutasa/metabolismoRESUMEN
Ferrous oxyhemoglobin (HbO2) in red blood cells (RBCs) invariably and slowly autoxidizes to form ferric methemoglobin (metHb). However, the level of metHb is always maintained below 0.5% by intracellular metHb reduction of enzymatic systems with coenzymes, such as reduced nicotinamide adenine dinucleotide (NADH), and by superoxide dismutase (SOD) and catalase (CAT), which eliminate reactive oxygen species. Unquestionably, NADH cannot reduce metHb without the corresponding enzymatic system. Our study, however, demonstrated that a high concentration of NADH (100-fold of normal level, equimolar to HbO2) retarded autoxidation of HbO2 in a highly purified Hb solution with no enzymatic system. Furthermore, an inhibitory effect of NADH on metHb formation was observed with additions of oxidants such as H2O2, NO, and NaNO2. Our mechanism assessment elucidated extremely high pseudo-CAT and pseudo-SOD activities of NADH with coexistence of HbO2, and reactivity of NADH with NO. We prepared a model of RBCs (Hb-vesicles, Hb-V) encapsulating purified HbO2 solution and NADH, but no enzymatic system within liposome. We confirmed the inhibitory effect of NADH on both autoxidation and oxidant-induced metHb formation. In addition, an intravenous administration of these Hb-Vs to rats caused significant retardation of metHb formation by approximately 50% compared to the case without NADH coencapsulation. Based on these results, we elucidated a new role of NADH, that is, antioxidative effect via interaction with Hb, in addition to its classical role as a coenzyme.
Asunto(s)
Antioxidantes , Hemoglobinas/química , Hemoglobinas/metabolismo , NAD/química , NAD/metabolismo , Animales , Antioxidantes/química , Antioxidantes/metabolismo , Peróxido de Hidrógeno/química , Modelos Moleculares , Óxido Nítrico/química , Oxidación-Reducción , Ratas , Nitrito de Sodio/química , Superóxido Dismutasa/metabolismoRESUMEN
BACKGROUND: Definitive substrates for the measurement of pancreatic lipase activity in human serum have not been conclusively identified owing to poor aqueous solubility and nonspecific susceptibility of substrates with existing methods. Thus, it is still important to propose new substrates for robust lipase measurements. METHODS: Reaction conditions were studied for a lipase method using newly synthesized 2,3-dibutyrylthio-1-propyl oleate as the substrate and 5,5'-dithio-bis-(2-nitro-benzoic acid) as the chromogen. RESULTS: Optimum conditions, using colipase and Mg(++) in aqueous hexamethyl phosphoric triamide medium at pH 9.2, were defined. The substrate was highly selective to pancreatic lipase. The reaction increased linearly with lipase concentrations up to 500 U/l. The reference interval of serum lipase concentrations was 21.5-65 U/l. Using the Passing-Bablok regression analysis, the present assay shows a slope of 0.414, an intercept of -2.4 U/l, and r-value of 0.992 in the comparison with the chromogenic method using the 6-methylresorufin ester of 1-O,2-O-dilauryl-rac-glycero-3-glutaric acid as the substrate. CONCLUSION: Because of the simple composition of the reagents, the proposed procedure may represent a significant advancement in the commercially available methods for pancreatic lipase determination.
Asunto(s)
Lipasa/sangre , Páncreas/enzimología , Pancreatitis/diagnóstico , Compuestos de Sulfhidrilo , Adulto , Ésteres/metabolismo , Femenino , Humanos , Concentración de Iones de Hidrógeno , Hidrólisis , Cinética , Lipasa/metabolismo , Masculino , Métodos , Persona de Mediana Edad , Estándares de Referencia , Compuestos de Sulfhidrilo/metabolismoRESUMEN
2,4-Dinitrophenyl-1-thio N-acetyl-beta-D-glucosaminide was examined as a new substrate for analyzing the level of N-acetyl-beta-D-glucosaminidase in the urine of patients suffering from renal diseases. The analysis is based on the fact that the substrate, when hydrolyzed in the presence of N-acetyl-beta-D-glucosaminidase, liberates 2,4-dinitrothiophenol as the chromogen. The optimum pH for the enzyme reaction is 4.6, which is covered by the optimal range for the UV absorbance of the chromogen. The first-order rate of increase of the absorbance at this pH was linear to the enzyme concentration up to 600 U/L, with a high sensitivity. Analytical reagents with glucosaminides of 2,4-dinitrophenol and 2-chloro-4-nitrophenol are less stable than the reagent with glucosaminide of 2,4-dinitrothiophenol. The optimum pH for the absorbance of p-nitrophenol and 2-chloro-4-nitrophenol does not match that for the enzyme reaction.