Downregulation of SOX9 expression in developing entheses adjacent to intramembranous bone.

Entheses are classified into three types: fibrocartilaginous, fibrous, and periosteal insertions. However, the mechanism behind the development of fibrous entheses and periosteal insertions remains unclear. Since both entheses are part of the temporomandibular joint (TMJ), this study analyzes the TMJ entheses. Here, we show that SOX9 expression is negatively regulated during TMJ enthesis development, unlike fibrocartilage entheses which are modularly formed by SCX and SOX9 positive progenitors. The TMJ entheses was adjacent to the intramembranous bone rather than cartilage. SOX9 expression was diminished during TMJ enthesis development. To clarify the functional role of Sox9 in the development of TMJ entheses, we examined these structures in TMJ using Wnt1Cre;Sox9flox/+ reporter mice. Wnt1Cre;Sox9flox/+ mice showed enthesial deformation at the TMJ. Next, we also observed a diminished SOX9 expression area at the enthesis in contact with the clavicle's membranous bone portion, similar to the TMJ entheses. Together, these findings reveal that the timing of SOX9 expression varies with the ossification development mode.

Fit and Retention of Cobalt-Chromium Removable Partial Denture Frameworks Fabricated with Selective Laser Melting.

PURPOSE: To evaluate fit and retention of cobalt-chromium removable partial denture (RPD) frameworks fabricated with selective laser melting (SLM). METHODS: Three types of framework for clasp-retained RPDs were virtually designed and fabricated using SLM (n = 30). For comparison, 30 additional frameworks were produced using conventional lost-wax casting. A biomechanical model was created, incorporating extracted teeth mounted on flexible metal posts. Using this model, horizontal constraint forces resulting from a misfit were measured using strain gauges, while vertical forces were not recorded. The constraint force components and resultant forces were determined for all abutment teeth, and the maximum retention force during RPD removal from the model was also assessed. For statistical evaluation, the two fabrication methods were analyzed by calculating the means and standard deviations. RESULTS: The average horizontal constraint forces showed similar values for both fabrication methods (SLM: 3.5 ± 1.0 N, casting: 3.4 ± 1.6 N). The overall scatter of data for cast RPDs was greater compared to those fabricated using SLM, indicating a better reproducibility of the SLM process. With regard to retention, the intended retention force of 5-10 N per abutment tooth was not attained in one of the cast groups, while it was consistently achieved in all SLM groups. CONCLUSIONS: This in vitro study found that SLM is a promising option for the manufacture of cobalt-chromium RPD frameworks in terms of fit and retention.

Diagnostic Accuracy of Mann Assessment of Swallowing Ability for Predicting Dysphagia in Patients with Psychiatric Disorders.

Dysphagia occurs in various diseases and constitutes a major concern in patients with psychiatric disorders. The Mann Assessment of Swallowing Ability (MASA) comprises 24 clinical parameters designed to identify swallowing disorders. One item in MASA, the "gag reflex", involves an unpleasant stimulus, which means that it is often omitted when the test is administered. The aims of this study were to determine the presence/absence of dysphagia in patients with psychiatric disorders using the MASA and determine its diagnostic accuracy when the gag reflex item was excluded in patients with psychiatric disorders. The study participants comprised patients admitted to a hospital psychiatric ward in whom dysphagia had been suspected based on oral intake status. The following items were determined: age, total MASA score (23 out of 24 items, giving a score out of 195 points), body mass index score, milligram equivalents of chlorpromazine, and the Food Intake Level Scale score. The patients were divided into two groups according to the presence or absence of swallowing problems as assessed by videoendoscopic or videofluoroscopic examination. The scores for each item investigated in the MASA, including the total score, were compared between the two groups. Receiver operating characteristic curve analysis was carried out to determine the optimum cut-off value. The total MASA score, which excluded the "gag reflex" item, was lower in the problematic swallowing group than in the non-problematic swallowing group. The MASA scores for cooperation, respiratory, dysphasia, tongue coordination, oral preparation, pharyngeal phase, and pharyngeal response tended to be lower in the problematic swallowing group. Furthermore, an optimum cut-off value of 169 points (sensitivity, 0.92; specificity, 0.68; likelihood ratio, 2.84) was identified. These results indicate that the cut-off MASA score is effective in screening for dysphagia, even when the "gag reflex" item is excluded.

Comparison between interocclusal registration using silicone bite registration material and intraoral scanner on clenching strength.

INTRODUCTION: The purpose of this study was to clarify the influence of clenching strength on interocclusal registration using intraoral scanner (IOS). METHODS: Subjects were eight volunteers. Two experimental conditions were light clenching (LC) and 40% maximum voluntary clenching (MVC). The conventional silicone bite registration and IOS were used for comparison. Occlusal contact areas (OCA) for different clenching strengths were compared, along with variation of measured values (VMV) between recording methods. RESULTS: Significant differences were observed between conditions on OCA and between methods on VMV. CONCLUSION: Clenching strength influenced interocclusal registration using IOS. Int J Prosthodont. 10.11607/ijp.8445.

Simultaneous prosthodontic treatment for crowns of natural teeth and dental implants using digital technology: A case report.

The aim of this clinical report was to describe the improvement of masticatory disorders with the use of digital technology to simultaneously perform prosthodontic treatment of natural teeth and edentulous areas. Computer-guided implant surgery was performed, and crown prostheses and implant superstructures were fabricated simultaneously using digital technology.

Effects of hollow structures added by selective laser sintering on the mechanical properties of Co-Cr alloy.

PURPOSE: This study investigates the effects of hollow structures, added by selective laser sintering (SLS), on the mechanical properties of a Co-Cr alloy for providing an optimal structural property to the framework components of removable partial dentures (RPDs). METHODS: The specimens produced using the 3D data of the dumbbell-shaped cylinders were divided into four groups based on the manufacturing method: Cast, Mill, SLS-solid, and SLS-hollow. Tensile tests were performed to measure the mechanical properties of the specimens. The mechanical property values among the four groups were statistically compared using the Kruskal-Wallis test followed by the Steel-Dwass test (α = 0.05). RESULTS: The median elastic modulus was the largest in the Cast, followed by SLS-solid, Mill, and SLS-hollow, with no significant differences observed between all conditions. The median ultimate tensile strength was the largest in the order of SLS-solid, Mill, SLS-hollow, and Cast. The median 0.2% proof stress was the largest in SLS-solid, followed by SLS-hollow, Cast, and Mill. The median elongation was the highest in the order of Mill, SLS-solid, SLS-hollow, and Cast. CONCLUSIONS: With the addition of hollow structures, the elastic modulus decreased while the mechanical strength and proof stress remained high in SLS specimens. In addition, the ISO 22674 standard for dental metals was met, suggesting that SLS may be a possible method to design RPD frameworks with high strength and optimal structural properties.

Digital technology for fabrication of removable dental prosthesis with double crowns combining fiber-reinforced composite and zirconia.

PATIENTS: This clinical report describes the process for fabricating a double-crown-retained removable dental prosthesis combining a fiber-reinforced composite and zirconia using digital technology. An 83-year-old woman presented with gingival swelling around the maxillary right premolar. The swollen tooth was the abutment tooth of a cross-arch fixed partial denture. An intraoral scanner (IOS) and computer-aided design/manufacturing as digital technology were used to plan treatment with a double-crown-retained removable dental prosthesis. A metal-free prosthesis using zirconia for the primary crown and fiberglass-reinforced composite resin for the secondary crown was planned, and the patient consented to the treatment plan. After autotransplantation of a tooth as one of the abutments, the IOS was used to obtain digital scans of the prepared surface of the abutment teeth, opposing dentition, and occlusal relationships. First, primary crowns were milled using zirconia. Next, the intraoral scanner obtained a pick-up impression of the primary crowns, and secondary crowns were designed and milled from the fiber-reinforced composite. After delivery, the patient expressed satisfaction with the functionality, esthetics, and fit of the double-crown-retained removable dental prosthesis. DISCUSSION: Digital technology offers many advantages such as efficient fabrication of double crowns, reduced material costs, improved biocompatibility, and good aesthetics of metal-free materials. CONCLUSIONS: This clinical report describes the application of digital technology for the fabrication of a double-crown-retained removable dental prosthesis combining a fiber-reinforced composite and zirconia, resulting in patient satisfaction.

A new proposal for improving the accuracy of intraoral scanning for partially edentulous residual ridge.

Purpose This study investigated the usefulness of a newly proposed intraoral scanning method, using markers that can be used directly in the oral cavity, in order to improve the accuracy of impression taking of the residual ridge for fabrication of removable partial dentures.Methods An intraoral scanner was used to scan a dental model of a partially edentulous mandibular arch (Kennedy Class I). As markers, pieces of dried pasta were used. The scanning operation was performed under three conditions. In Condition 1, scanning was performed on the remaining teeth and the residual ridge without markers. In Condition 2, scanning of the remaining teeth and residual ridge was performed with markers. In Condition 3, the markers were removed from the model used in Condition 2, and the residual ridge was scanned again. The scanning data of each condition was superimposed on the control data, and the shape error was calculated and compared among the conditions.Results There was a significant difference in trueness of the residual ridge before and after marker application. The application of markers improved the trueness, while maintaining precision. Re-scanning after removing the marker did not affect trueness between before and after re-scanning and the re-scanned region showed shape continuity with the surrounding region.Conclusions The present method using markers that can be used in the oral cavity was effective in improving the accuracy of impression taking at the residual ridge.

A Study for Determining the Inclination of the Occlusal Plane from the Mandibular Functional Trajectory.

Background: During the fabrication of prostheses, the putative occlusal plane is generally determined based on morphological criteria such as Camper's plane. There is a possibility that morphological indexes deviate from their original positions over time. The current study investigated whether functional indexes could be used to determine occlusal planes instead of morphological indexes. Objectives: The objectives of the present study were to investigate the relationship between the maxillary occlusal plane and mandibular functional trajectory and to consider a method for reconstructing a lost occlusal plane based on functional trajectories. Methods: Thirteen healthy dentulous individuals were recruited for the study. Using a jaw motion tracking device with 6-degrees of freedom, the trajectories of the mandibular first molar and incisor during masticatory movement or tapping movements were measured. Results: The closing angle of the mandibular first molar to the maxillary occlusal plane was converged and averaged 74.1° just below the intercuspal position, which is the terminal position of masticatory movement. This angle was positively correlated with the closing angle of the incisal point during tapping movement. The regression equation obtained was y = 0.37x + 45.99, where x was the closing angle of tapping movement and y was the closing angle of masticatory movement. Conclusions: It was suggested that when determining lost occlusal plane, the occlusal plane can be derived using the closing angle of the incisal point during tapping movement.

Retentive force of conical crowns combining zirconia and fiber-reinforced composite.

PURPOSE: This study investigated the retentive force of conical crowns combining zirconia primary and fiber-reinforced composite (FRC) secondary crowns and their changes due to aging. METHODS: Zirconia primary crowns were produced with a convergence angle of 3°. Thirty-two secondary crowns were milled from FRC and divided into two groups (n = 16/group) based on the polishing method of the secondary crown inner surfaces: diamond paste (Group 1) and silicone points (Group 2). After fitting the secondary crowns with different fitting forces (F), loosening forces (L) were determined. Tests were repeated after an occlusal stop (OS) was added to the secondary crown and artificial aging (10,000 insertion/removal cycles). Data were compared using the Wilcoxon and Mann-Whitney U tests. RESULTS: Crowns without an OS showed L/F ratios of 0.4586 (Group 1) and 0.4104 (Group 2). With an OS, maximum retention was not significantly affected by the polishing method and could be limited to Lmax = 19.31±7.77 N (Group 1) and Lmax = 16.12±5.92 N (Group 2). CONCLUSIONS: These findings suggest that the combination of conical zirconia primary and FRC secondary crowns can obtain acceptable retentive forces that are not affected by aging if the inner surfaces are polished with diamond paste. OS generation could limit maximum retention, but should be adjusted if the target value of 10 N is not to be exceeded. With a change of the convergence angle to 4°, L/F values for crowns without an OS would be close to 1/3, which is considered ideal for conical crowns. CLINICAL SIGNIFICANCE: The combination of zirconia primary crowns and FRC secondary crowns was found feasible to ensure the required retention for clinical use over a long time span. Furthermore, it offers an alternative to metal-based restorations while ensuring high levels of biocompatibility and esthetics.

Influence of molding angle on the trueness and defects of removable partial denture frameworks fabricated by selective laser melting.

PURPOSE: To examine the effect of molding angle on the trueness and defects associated with removable partial denture (RPD) frameworks fabricated by selective laser melting (SLM). METHODS: A plaster model of a partially edentulous mandibular arch classified as Kennedy class II modification 1 was used. After obtaining the 3D data of the model (design data), a framework was designed using CAD software. Based on the design data, three different molding angle conditions (0°, 45°, and -45°) were set in the CAM software. The frameworks were fabricated by SLM under each condition, and 3D data were captured (fabrication data). The design and fabrication data were superimposed using 3D inspection software to verify the shape errors. The number of support structures was then measured. To examine the internal defects, micro-computed tomography (µCT) was performed for void analysis. Surface roughness was measured using a laser microscope. RESULTS: The overall shape errors of the RPD framework were smaller under the 0° condition compared with the others, and the largest number of support structures was observed at 0°. Many internal defects were observed in the large components of the framework at 45° and -45°. The surface roughness was the smallest at -45°. CONCLUSION: The trueness and defects associated with the RPD frameworks were affected by the difference in the SLM molding angle.

Analysis of the relevant factors associated with oral health-related quality of life in elderly denture wearers.

PURPOSE: The purpose of the present study was to explore the factors related to oral health-related quality of life (OHRQoL) in elderly patients who underwent prosthetic treatment with removable dentures through several objective and subjective examinations and to analyze their interrelationships. METHODS: The current study involved 78 denture wearers, aged 65 years or above, who underwent treatment at the clinic of prosthodontics and undertook routine checkups. The present study used eight age-matched patients with healthy dentition as controls. The following items were evaluated: OHRQoL (Oral Health Impact Profile), main occluding area, maximal occlusal force, masticatory performance, and masticatory ability. Spearman's rank correlation coefficient was used to assess the correlation between the OHIP score and each item and multiple linear regression analysis with the backward elimination method was used to analyze the factors affecting the OHIP score (α = 0.05). RESULTS: The OHIP summary score was significantly correlated with the number of occlusal supports, number of remaining teeth, maximal occlusal force, and the masticatory ability score. The multiple regression analysis revealed that the number of occlusal supports, the number of remaining upper teeth, whether the upper main occluding area was the remaining tooth or not, and the masticatory ability score were the significant independent variables that affected the OHIP summary scores. CONCLUSION: The present study verified the relationship between the OHRQoL and the occlusion of remaining teeth or the items derived from the main occluding areas. The current results suggest that retaining the maxillary first molar is a key factor in the OHRQoL.

Intraoral scanner and computer-aided design/manufacturing technology for the fabrication of double-crown-retained removable dental prosthesis: A clinical report.

PATIENT: This clinical report describes treatment with a double-crown-retained removable dental prosthesis fabricated using an intraoral scanner (IOS) and computer-aided design/manufacturing technology (CAD/CAM). A 68-year-old female patient presented with complaints of missing maxillary right first and left second premolars. CAD/CAM technology was applied to plan treatment with a double-crown-retained removable dental prosthesis. The patient consented to this treatment option but did not want orthodontic treatment for the anterior crossbite of the right side. After the definitive preparation of the abutment teeth, the shape of the provisional restoration was adjusted to match the definitive prosthesis. An IOS was used to obtain digital scans of the provisional restoration, occlusion, antagonist arch, and prepared surface of the abutment teeth. First, the primary crowns were milled from cobalt-chromium alloy. Next, using an intraoral scanner, a pick-up impression of the primary crowns was performed, and the secondary crowns were designed, milled, and veneered. After delivery, the patient expressed satisfaction with the functionality, esthetics, and fit of the double-crown-retained removable dental prosthesis. DISCUSSION: The surface of the primary crowns was coated with scan spray when the pick-up impression was made using the IOS. Practice is needed to achieve a thin and homogeneous coating with scan spray to improve reproducibility. CONCLUSION: Double-crown-retained removable dental prostheses can be successfully fabricated using an IOS and CAD/CAM technology, resulting in patient satisfaction.

Retentive force of telescopic crowns combining fiber-reinforced composite and zirconia.

PURPOSE: This study investigated changes in the retentive force of telescopic crowns fabricated by combining a zirconia primary crown and a fiber-reinforced composite (FRC) secondary crown. METHODS: Primary zirconia crowns were produced with a nominal convergence angle of 0°. Forty-eight secondary crowns were milled from FRC and divided into three study groups (n=16/group) based on milling parameters and post-milling adjustment. The offset parameter used for the final milling step of the inner crown surface was adjusted for a tight initial fit in Group 1 (milling offset: +10 µm, i.e., 2 × 10 µm = 20 µm lower inner diameter compared with the CAD file of the crown) and for improved initial fit (milling offset: -10 µm, i.e., an enlargement of the inner crown diameter by 2 × 20 µm = 40 µm in relation to Group 1) in Groups 2 and 3. The inner surfaces of the secondary crowns were polished with diamond paste in Groups 1 and 2, and silicon points were used for Group 3. The retentive force was measured using a universal testing device. The secondary crown was placed on the primary crown, with the final fitting force set to a load of 100 N. This test was conducted before and after aging (10,000 insertion/removal cycles) under dry and wet conditions. A generalized linear model was used to estimate the differences in the retentive force to elucidate the effects of the milling parameters and polishing methods. RESULTS: We realized an initial retentive force of approximately 10 N. In Groups 2 and 3, the difference was statistically significant between the dry and wet conditions before aging (P < 0.05). There was no significant difference between the dry and wet conditions after aging in any of the groups (P > 0.05). CONCLUSION: An adequate initial retentive force can be achieved with telescopic crowns combining zirconia and FRC.

Fabrication of Zirconia Abutment Crown and Clasp Under Existing Removable Partial Denture Using CAD/CAM Technology.

This clinical report describes a technique for fabricating a retrofit zirconia crown and clasp for an existing removable partial denture (RPD) using computer-aided design and computer-aided manufacturing (CAD/CAM). A 58-year-old patient developed acute pulpitis of a tooth, and the RPD clasp was broken on the tooth. A pre-preparation scan was captured using an intraoral scanner. The existing RPD was placed in the mouth, and scans of the post-preparation, antagonist arch, and interocclusal record were made. A zirconia crown was designed by superimposing the pre-preparation scan and the post-preparation scan of the abutment tooth. The design data were transferred to a 5-axis milling machine, and the crown was milled from zirconia. The crown was luted using resin cement. An intraoral scan of the crown with RPD was taken, and the RPD was removed to re-scan the proximal guide plane of the crown. The I-bar clasp was designed, and the casting pattern was built using a 3D printer. The pattern was used to cast the clasp. The clasp was set using autopolymerizing acrylic resin to the RPD. The main advantages of this efficient process are that patients can use their RPDs during fabrication of the crown of the abutment tooth. The limitations of using this technique include the cost of the intraoral scanner and the education for the dental clinicians and technicians. In this clinical report, CAD/CAM technology prevented human error, required no adjustment, and uninterrupted use of the RPD for fabricating a retrofit zirconia crown and clasp for an existing RPD.

Influence of Simulated Loss of Posterior Occlusal Support on Three-dimensional Condylar Displacement.

The objective of this study was to verify whether loss of posterior occlusal support induced displacement of the mandibular condyles from a physiological point of view. Stabilization-type splints were fabricated for 12 healthy dentulous individuals. Each splint was designed to cover the bilateral maxillary teeth up to the second molars. To reproduce loss of posterior occlusal support, the extent of the splint was reduced one tooth at a time, bilaterally, starting from the back and moving forward sequentially. Tapping movement and lateral excursions were performed with each splint and a jaw movement tracking device with 6-degrees of freedom used to observe condylar displacement. Evaluation of 3-dimensional (3-D) displacement of the kinematic axis of the condyle during experimental jaw movement was performed under each occlusal condition with occlusal contact on all teeth, including the maxillary second molars. The habitual closing position was used as the reference. An increase was observed in 3-D displacement of the kinematic axis at the terminal point of the tapping movement with loss of occlusal support, and significant differences were observed in both condyles. An increase was also observed in 3-D displacement of the kinematic axis on the working side during lateral excursion with loss of occlusal support, and a statistically significant difference was observed in the left condyle. A small increase was observed in 3-D displacement of the kinematic axis on the non-working side during lateral excursion with loss of occlusal support. The results of this study suggest that loss of posterior occlusal support induces displacement of the mandibular condyles, suggesting that occlusal support in the molar region is an important factor in stabilization of the condylar position.

Large-Conductance Calcium-Activated Potassium Channels and Voltage-Dependent Sodium Channels in Human Cementoblasts.

Cementum, which is excreted by cementoblasts, provides an attachment site for collagen fibers that connect to the alveolar bone and fix the teeth into the alveolar sockets. Transmembrane ionic signaling, associated with ionic transporters, regulate various physiological processes in a wide variety of cells. However, the properties of the signals generated by plasma membrane ionic channels in cementoblasts have not yet been described in detail. We investigated the biophysical and pharmacological properties of ion channels expressed in human cementoblast (HCEM) cell lines by measuring ionic currents using conventional whole-cell patch-clamp recording. The application of depolarizing voltage steps in 10 mV increments from a holding potential (Vh) of -70 mV evoked outwardly rectifying currents at positive potentials. When intracellular K+ was substituted with an equimolar concentration of Cs+, the outward currents almost disappeared. Using tail current analysis, the contributions of both K+ and background Na+ permeabilities were estimated for the outward currents. Extracellular application of tetraethylammonium chloride (TEA) and iberiotoxin (IbTX) reduced the densities of the outward currents significantly and reversibly, whereas apamin and TRAM-34 had no effect. When the Vh was changed to -100 mV, we observed voltage-dependent inward currents in 30% of the recorded cells. These results suggest that HCEM express TEA- and IbTX-sensitive large-conductance Ca2+-activated K+ channels and voltage-dependent Na+ channels.

Accuracy of 3D printing compared with milling - A multi-center analysis of try-in dentures.

OBJECTIVES: In recent years, computer-aided design/computer-aided manufacturing (CAD/CAM) has been used to produce removable complete dentures. Most workflows include fabrication of milled or 3D-printed try-in prostheses. 3D-printing accuracy is affected by laboratory-specific and operator-dependent factors. This international five-center study sought to compare the accuracy of 3D-printed and milled try-in dentures. METHODS: The construction file of a maxillary removable complete denture was selected as a reference. Eight try-in dentures were 3D printed at each of the five centers. Each center used their own printer (Objet260 Connex, Stratasys; MAX, Asiga; Anycubic Photon, Anycubic 3D; PRO2, Asiga and cara Print 4.0, Kulzer) along with their own material, printing settings, post-processing and light-curing parameters. At center 2, eight try-in dentures were milled to serve as a benchmark (PrograMill PM7, Ivoclar Vivadent). Dentures were scanned and aligned to the reference file using best-fit algorithms. Geometric accuracy was analyzed using the root mean square value (trueness) and standard deviation (precision) of the distributed absolute mesh deviations. Mean values of the five sets of printed dentures and the single set of milled dentures were compared. RESULTS: Milled dentures showed a mean trueness of 65 ±â€¯6 µm and a mean precision of 48 ±â€¯5 µm. Thus, they were significantly more accurate than the 3D-printed dentures in four out of five centers. In mean absolute numbers, 3D printing was less true than milling by 17-89 µm and less precise by 8-66 µm. CONCLUSIONS: Although milling remains the benchmark technique for accuracy, differences between milled and 3D-printed dentures were non-significant for one printing center. Furthermore, the overall performance of 3D printing at all centers was within a clinically acceptable range for try-in prostheses. CLINICAL SIGNIFICANCE: The accuracy of 3D printing varies widely between and within laboratories but nonetheless lies within the range of accuracy of conventional manufacturing methods.

Effects of repetitive insertion/removal cycles and simulated occlusal loads on retention of denture retainers.

The purpose of this study was to investigate the effects of repetitive insertion/removal cycle tests on denture retainers with simulated occlusal loads on the retentive force and deformation of clasp. Abutment teeth in the form of mandibular secondary premolars and clasp in the form of Akers clasps were prepared. The retentive force of the clasp on the abutment teeth were evaluated before and after undergoing repetitive insertion/removal cycle tests with or without cyclic loading. Changes in the clasp shape were monitored using a 3D scanner and scanning electron microscope. The initial retentive force was approximately 10 N and this value later decreased due to deformation of the clasp tips. In contrast to the non-load group, the load group exhibited a reduction in retentive force during earlier stages. Therefore, cyclic loading was related to a decrease in retentive forces, specifically in the early stages of repetitive insertion/removal cycles.