Rapid warning of emerging contaminants in reuse water using biocathode sensors.

The proliferation of emerging contaminants (ECs) in the environment poses a major threat to the safety of reuse water. However, many ECs exist for which no corresponding control standards have been established. Here, we used polarity reversal to construct a biocathode sensor capable of early warning of ECs biotoxicity in aerobic reuse water with low organic concentrations. The baseline current and sensitivity of the biosensor in response to formaldehyde were enhanced by 25% and 23% using microbial fuel cell effluent as the inoculum. The microbial community explained that the inoculum primarily influenced the performance of the biosensor by modulating species abundance, function and interactions. More importantly, the successfully commissioned biocathode sensor demonstrated rapid warning capability (Response time less than 1.3 h) for ECs such as fluoride, disinfection by-products and antibiotics in an actual landscape reuse system. Further, the sensor could quantify the concentration of a single known contaminant. Our study demonstrated a method for rapid early warning of ECs in an oxygen-rich, low-organics environment, promoting innovative development of monitoring technologies for water ecology and environmental safety.

Self-organized mud cracking amplifies the resilience of an iconic "Red Beach" salt marsh.

Self-organized patterning, resulting from the interplay of biological and physical processes, is widespread in nature. Studies have suggested that biologically triggered self-organization can amplify ecosystem resilience. However, if purely physical forms of self-organization play a similar role remains unknown. Desiccation soil cracking is a typical physical form of self-organization in coastal salt marshes and other ecosystems. Here, we show that physically self-organized mud cracking was an important facilitating process for the establishment of seepweeds in a "Red Beach" salt marsh in China. Transient mud cracks can promote plant survivorship by trapping seeds, and enhance germination and growth by increasing water infiltration in the soil, thus facilitating the formation of a persistent salt marsh landscape. Cracks can help the salt marsh withstand more intense droughts, leading to postponed collapse and faster recovery. These are indications of enhanced resilience. Our work highlights that self-organized landscapes sculpted by physical agents can play a critical role in ecosystem dynamics and resilience to climate change.

Self-forming electroactive dynamic membrane for enhancing the decolorization of methyl orange by weak electrical stimulation.

An electroactive dynamic membrane (EADM), which enabled simultaneous solid-liquid separation and contaminants removal, has been developed by electrostimulation using domestic wastewater as inoculum. Results showed that both the control dynamic membrane (CDM), without electrical stimulation, and the EADM systems exhibited stable removal performance with chemical oxygen demand (COD), and a robustness in responding to a fluctuating organic load. With the introduction of a weak electrical field, the EADM transmembrane pressure (TMP) was significantly reduced (0.02 kPa/d) compared with the control (0.20 kPa/d). In the treatment of methyl orange (MO), the EADM system achieved a decolorization efficiency of 85.87 %, much higher than the control dynamic membrane (CDM) system (58.84 %), which can be attributed to electrical stimulation and H2 production on cathode. Microbial analysis has established that electrostimulation enriched the electroactive bacteria in the dynamic biofilm, and shaped the microbial structure, with improved contaminant removal. The results of this study highlight the potential of regulating the microbial community and creating a beneficial biofilm as a dynamic layer to facilitate contaminant removal.

Saltmarsh resilience controlled by patch size and plant density of habitat-forming species that trap shells.

Habitat fragmentaion into small patches is regarded as a vital cause of biodiversity loss. Fragmentationof habitat-forming species is especially harmful, as patchiness of such species often controls ecosystem stability and resilience by density and patch size-dependent self-reinforcing feedbacks. Although fragmentation are expected to weaken or even break such feedbacks, it remains unclear how the resulting patchiness of habitat-forming species affect ecosystem resilience to environmental stresses. Here, using Spartian alterniflora, the habitat-forming species in saltmarshes as a model, we investigate how patch size, plant density, and shell aggregation interactively control the persistence of a degrading salt marsh that suffered from erosion induced by hydrodynamics. Our results demonstrate that large patches can trap more shells along the patch edge than the smaller ones, therefore significantly facilitating plant re-growth within the patch. Shell removal experiments further reveal that large patches trapping more shells along patch edges reinforce their own persistence by decreasing erosion and thus facilitating plant recovery. By contrast, small patches with lesser plants cannot persist as they trap less shells along patch edges but are able to accumulate more shells at interior locations where they hinder plant re-growth, indicating a critical threshold of patch size ~20 m2 below which ecosystem collapses. The current study highlights the importance to identify critical threshold of stress-resistant patch sizes in transition-prone ecosystems as early-warning to alert undesired ecosystem collapse and restoration practice.

Effects of interactions between macroalgae and seagrass on the distribution of macrobenthic invertebrate communities at the Yellow River Estuary, China.

Algae-dominance in seagrass beds has been well recognized, however, the competitive relationship between seagrass and macroalgae along land-sea gradients and their ecological effects has received little attention. In this study, a field survey was conducted at the Yellow River Estuary to investigate the effects of macroalgal proliferation on seagrass and macrobenthic invertebrate communities. Our results suggested that strong competitive interaction existed between the two primary producers, and the positive or negative effects of macroalgae on seagrass growth varied along land-sea gradient. Furthermore, the dominant controlling factors on the biomass, density and diversity of macrobenthic invertebrate communities were found to vary accordingly, i.e., from features of the primary producers in the nearshore where macroalgae suppressed seagrass growth to hydrodynamic disturbance in the offshore where macroalgae facilitated seagrass growth. Our study emphasizes the importance to integrate interspecific competition into ecosystem-based management of seagrass ecosystem, and provides references for additional ecological indicators.

Biogeomorphological processes and structures facilitate seedling establishment and distribution of annual plants: Implications for coastal restoration.

Biogeomorphological processes and structures (BPS) can affect plant growth and community structure and promote landscape complexity in ecosystems. However, there is a lack of understanding of how BPS facilitates seedling establishment and distribution of annual plants and promotes the success of coastal restoration. We studied the relationships between seedling establishment of a native annual plant species (Suaeda salsa) and BPS resulting from crabs and plants in a middle elevation salt marsh with moderate tides (where inhabited generally high density of plants and crabs) in the Yellow River Delta of China. While there were many crabs but fewer plants in lower elevation areas with more frequent and stronger tides; and in higher elevation areas with weaker tides there were both fewer crabs and plants. Investigations and field manipulation experiments of microtopography, crabs and plants were conducted to determine if and how these BPS influenced seedling establishment and distribution under tidal influence in the middle elevation salt marshes. Results demonstrated that biogeomorphological structures, mainly concave hollows generated by crab burrowing and concave hollows around plant roots and stems under tidal influence, were associated with the trapping of seeds and influenced the establishment and distribution of seedlings. Additionally, upon senescence, maternal plants with unreleased seeds lodged on the ground and influenced seed retention and seedling establishment. The artificial concave hollows that were created experimentally also trapped many seeds and facilitated seedling establishment. Experimental plantings and creation of artificial hollow microtopography attracted crabs that created burrows, resulting in a positive feedback on seedling establishment. We used information obtained from the experimental component of the study to conduct a hollow microtopography manipulation to successfully restore degraded salt marshes. Understanding the associations between seedling establishment and biogeomorphological processes provides important insights for the utilization of natural or human ecosystem engineering to restore coastal vegetation ecosystems.

The impact assessment of hydro-biological connectivity changes on the estuary wetland through the ecological restoration project in the Yellow River Delta, China.

Yellow River Delta (YRD) is one of the youngest delta with complex hydrological and biological connectivity in the world, where offers habitats to the famous waterfowls in the Eastern Asia. Meanwhile, one specific ecological restoration project named as the "Wuwanmu" and followed by the "Shiwanmu" within the National Nature Reserve of the Yellow River Delta (NNRYRD) complicated the hydrological and biological connectivity. How to quantitatively evaluate the extent of coastal wetland affected by the project will be a difficult problem. Hence the authors presented three innovative models of the Marine Connectivity Change Index (MCCI), the Coupling Index of Hydro-biological Connectivity (CIHBC), and the Assessment Index of Suitability on Bird Habitats (AISBH). After the project, the habitat of Phragmites australis has been restored effectively with the increased area of 24.59%, while the habitat of Suaeda salsa as the native species lost largely with decreased area of 84.62%. And the tidal channel having been cut off by the project resulted in isolating the buildup restoration area from seawater, and reshaping completely the plant habitat environment. So the hydrological and biological connectivity has been changed largely with the 47.79% decreased MCCI area and the 16.3% decreased zero-valued CIHBC area. However the AISBH non-zero-valued area increased 10.7%, and with the hidden worry of the decreased Grallatores number. From the connectivity prospective, three models presented a significant methodology to evaluate the complex impact on the estuary wetland habitat caused by the restoration project. In the long run, the ecological impacts should be highlighted to the change of tidal channel and the corresponding tidal issues, and the continuous and big loss of native plant spices such as S. salsa. The further study needs to explore the longer-term assessment of the ecological restoration project and its multiple effect in the future.

An integrative perspective to understand the impact of co-occurring ecosystem engineers on macroinvertebrates.

Invasion of habitat-modifying nonnative species and alteration of ecosystem engineer by exploitation are two of the dominant human impacts on natural ecosystem functioning. The effects of these co-occurring ecosystem engineers may act simultaneously and vary independently depending on ambient environmental conditions they modify. Using a saltmarsh ecosystem with continuing invasion of nonnative cordgrass and aggregation of native bivalve shells, we tested whether the ecosystem engineering effects of shell aggregation on macroinvertebrates depended on the plants due to their ability to trap shell debris. We found habitats covered with shell aggregation and vegetated cordgrass significantly increase the biodiversity but decrease the biomass of macroinvertebrates comparing to the tidal bare flats, whereas no differences were detected among shell covered, cordgrass vegetated and their coexistent habitats. Our study highlights the importance of considering multiple, potentially conflicting management goals, which may require flexibility and trade-offs to integrate nonnative and native resources into ecosystem management.

Intensive land uses modify assembly process and potential metabolic function of edaphic bacterial communities in the Yellow River Delta, China.

Coastal reclamation is a global threat to natural ecosystems, disturbing biological community structure, diversity and ecological function through habitat conversion. We have limited insights into the changes brought about by coastal reclamation for different land-use types. We used the Yellow River Delta (YRD) as a model because it is a region with intensive land reclamation, and we investigated the structural and functional variations of bacterial communities and their relations to edaphic properties under different land-use types. Our results showed that the high soil organic carbon (SOC), nitrate concentrations and salinity were found in oil field, aquaculture pond and salt pan, respectively, and low values in natural wetland. Land use was found to have significant influence on bacterial community diversity. To investigate the phylogenetic conservation of specific traits, we analyzed the relationship between soil bacterial assembly processes and edaphic properties. Bacterial traits phylogenetically conserved, and differs in depth. Our findings suggest that SOC served as a deep trait due to it negative correlation with deeper branches of phylogenetic clustering, while nitrate functioned as a shallow trait due to its positive correlation with phylogenetic clustering at finer branches. Soil salinity acted as a complex trait effected on both finer and deeper branches. Further potential functional gene co-occurrence network analysis revealed that land reclamation induced shifts of metabolic function by altering the functional gene connectivity. We found that the photosynthesis pathway was enriched in hub modules related to oil field (OF), while methane metabolism was enriched in hub modules linked to sea cucumber pond (CP1). In addition, two-component systems (TCS) were enriched with nitrate, ammonia, SOC and salinity-related modules. Therefore, our study highlights the importance of integrating multi-function and multi-process identification and prediction of coastal diverse reclamation impacts on coastal ecosystems.

How vegetation influence the macrobenthos distribution in different saltmarsh zones along coastal topographic gradients.

Macrobenthos are a key constituent of coastal salt marsh ecosystems and have often been used as sensitive indicators of the environment quality. In this study, field investigations in vegetated regions and adjacent bare patches of low, middle and high marshes were conducted to explore whether and how vegetation influence the macrobenthos distribution in different saltmarsh zones along coastal topographic gradients. Results showed that vegetation positively or negatively influenced macrobenthos mainly by changing their environment in different saltmarsh zones, as there were different degrees of physical stresses and food supply for the macrobenthos from the low to the high marsh. In the low marsh, no major differences in macrobenthos indices between the bare and vegetated sediments were found, yet density and biomass of the molluscs were higher in the bare patches. In the middle marsh, vegetation promoted the biomass and diversity indices but not the density and species richness of the macrobenthos, and vegetation was beneficial for some types of crustaceans but was detrimental for some types of polychaetes. In the high marsh, vegetation promoted the biomass, density, and species richness of the macrobenthos compared to those of adjacent bare patches. Coastal topographic gradients also had effects on macrobenthos distribution consistently with different habitat preferences and ecological niches, and the low marsh had the highest species richness, Shannon diversity, Pielou evenness and Margalef richness. This study also provided scientific implications for the management and restoration of the ecosystems in different intertidal saltmarsh zones.

Trait and density responses of Spartina alterniflora to inundation in the Yellow River Delta, China.

Understanding plant traits in response to physical stress has been an important issue in the study of coastal saltmarshes. For plants that reproduce both sexually and asexually, whether and how seedlings (sexual reproduction) and clonal ramets (asexual reproduction) may differentially respond to tidal inundation is still unclear. We investigated the growth and morphology of sexual and asexual propagules of an exotic saltmarsh plant (Spartina alterniflora) along a gradient of tidal submergence in the Yellow River Delta. Our results showed that the density, height and basal diameter of clonal ramets or sexual seedlings increased with tidal inundation. The patch amplification edge clonal ramets are superior than patch center plants. The differences response of plants to tidal inundation highlight the sensitivity of S. alterniflora to future tidal regime shifts and can help predict and evaluate the impacts of changes in inundation conditions due to sea level rise, coastal erosion and human activities.

River network connectivity and fish diversity.

Frequent and severe disruptions of natural river flows associated with human activities significantly alter hydrological connectivity in large river networks, with deleterious effects on fish diversity. Understanding the relationship between fish diversity and river network connectivity is fundamental to ensuring species persistence, ecosystem integrity, and human well-being. Here, we provide a review of the mechanisms by which river network connectivity (RNC) affects fish diversity. We review the relationships between forms, systems and types of RNC and fish diversity, based on more than 100 previous studies. In summary, sustaining RNC promotes fish diversity in longitudinal and lateral axes, and species sorting, dispersal dynamics, and habitat availability are the main factors driving the distribution of fish diversity, followed by nutrition and trophic dynamics. Our work highlights the effects of RNC on fish diversity, and provides a mechanistic understanding of how RNC affects fish diversity across river basins, thus providing scientific guidance for protecting fish biodiversity and improving the health of river network ecosystems.

Functional consumers regulate the effect of availability of subsidy on trophic cascades in the Yellow River Delta, China.

Understanding the environmental context where heterogeneous ecological processes affect biotic interactions is a key aim of ecological research. However, mechanisms underlying spatial variation in trophic interactions linked to resource availability across ecosystem gradients remains unclear. We experimentally manipulated the interactive effects of predator fish and quantitative gradient of leaf detritus on macroinvertebrates and benthic algae. We found that non-linear changes in the strength of trophic cascades were strongly linked to the retention rates of experimental leaf detritus and also determined by predatory consumers. Retention rate of leaf detritus influenced the recruitment of predatory invertebrates and foraging preference of predators, accounting for largely the variations in shift of strengthening and weakening trophic cascades. Our results highlight the importance to identify joint processes of recruitment and foraging responses of functional consumer in understanding the impacts of both anthropogenic and natural alterations in subsidy on trophic interaction of coastal food webs.

Microtopographical modification by a herbivore facilitates the growth of a coastal saltmarsh plant.

Increasing evidence shows that herbivores can facilitate plant growth and maintain the resistance of plant communities to trophic consumption in a variety of ecosystems. However, the positive effects of herbivores on annual saltmarsh plants in coastal ecosystems are relatively understudied. In this study, field investigations and manipulative experiments were conducted to explore whether and how microtopographical modification by the herbivorous crab Helice tientsinensis stimulates the growth of the saltmarsh plant Suaeda salsa. Results showed that, despite grazing on S. salsa, H. tientsinensis can promote density, total biomass, average plant height, average root length, and average biomass through burrowing-generated concave-convex microtopography, which can improve the edaphic environment (decreased soil hardness and salinity, and increased soil moisture content, oxidation-reduction potential, and carbon and nitrogen content), and provide plants more clustered growth opportunities that could facilitate positive intraspecific plant interactions. This study can provide scientific guidance for ecosystem restoration in coastal intertidal saltmarshes.

[Characteristics of net phytoplankton community and their relationships to environmental factors in the waters around Nansha Islands].

Based on samples collected in the waters around Nansha Islands from August 25 to September 28, 2011, the characteristics of net phytoplankton community and their relationships to environmental factors were investigated. A total of 113 species, belonging to 34 genera of 3 phyla were identified, among which 57.5% belonged to Pyrrophyta and 40.7% belonged to Bacillariophyta. Ceratium in Pyrrophyta had the most species accounting for 30.1% of the 113 species. The average abundance of net phytoplankton was 2.12 x 10(4) cell x m(-3) and high abundances were encountered in the complex gyre adjacent to Reed Tablemount and in the Cyclonic Gyre adjacent to Wan'an Tan. Trichodesmium in Cyanophyta was the dominant functional group, taking up 77.0% of the total net phytoplankton abundance. Trichodesmium thiebautii, T. erythraeum and Pyrocystis noctiluca were the major dominant species. The dominant species varied with locations. Cyanophyta widely dominated at stations 3, 5, 6 and 10-14, Pyrrophyta were the dominant phytoplankton in the central locations at stations 4 and 7-9, while Bacillariophyta dominated only at the southernmost stations 1 and 2. The values of Shannon index and Pielou evenness index of net phytoplankton community were 3.10 and 0.62, respectively. The salinity, water temperature, contents of ammonium, nitrite, phosphate and silicate, as well as mesoscale gyres and the west Nansha coastal current were the important environmental factors affecting the characteristics of net phytoplankton community. The ordination plots by canonical correspondence analysis could well display the characteristics of net phytoplankton community and their relationships to environmental factors.