[Study on the effect of electric field on the secondary structure of SOD by FTIR spectroscopy].

After the SOD (superoxide dismutase) was treated with different strength of electric field, the effect of electric field on the secondary structure of SOD was studied by FTIR (Fourier-transformation infrared spectroscopy). The results have shown that different electric field strength has different effects on the secondary structure contents of the SOD. As compared with the contrast, the relative contents of alpha-helix and beta-sheet were decreased, while beta-turn content increased. The random coil contents were increased except for the 1.0 kV x cm(-1). The electric treatment tends to transform the alpha-helix and beta-sheet into beta-turn and random coil, and different strength of electric field has a different effect on the transformation. The changes in SOD activity have a direct relationship to the contents of beta-sheet.

[Study on the effect of electric field on the secondary structure of HRP by circular dichroism].

After the HRP was treated with different strength of electric field, the effect of electric field on the secondary structure of HRP was studied by circular dichroism. The results show that different electric field strength ranging from 1.0 to 6.0 kV x cm(-1) has a different effect on the relative contents of alpha-helix, beta-sheet, beta-turn and random coil of the HRP. As compared with the contrast, the relative contents of alpha-helix, beta-turn and random coil decreased by 0.3%-11.4%, 1.7%-15.3% and 0.9%-20.4% respectively, while beta-sheet content increased by 0.9%-82.7%. The electric treatment tends to transform the alpha-helix, beta-turn and random coil into beta-sheet for the HRP. The result of this study has important meaning to explain the biological effect of electric treatment seeds.

[Study on the effect of electric field on the secondary structure of lipase by circular dichroism].

After the lipase was treated with electric field for five minutes, the effect of electric field on the secondary structure of lipase was studied by circular dichroism (CD). The results showed that different electric field strength in the range from 0. 5 to 6. 0 kV x cm(-1) has a different effect on the relative contents of alpha-helix, beta-sheet, beta-turn and random coil of the lipase, and whose changes were non-monotonous with the raising of electric field. In general, the electric treatment tends to transform the alpha-helix and beta-sheet into beta-turn and random coil. The decrease in alpha-helix and beta-sheet ranged respectively from 4. 6% to 48. 0% and from 13. 2% to 35. 1%, and the increase in beta-turn and random coil ranged respectively from 2. 8% to 33. 3% and from 0. 9% to 48. 1%. The result of this study has important meaning to explain the biological effect of electric treatment seeds.