Effects of Different Feed Additives on Intestinal Metabolite Composition of Weaned Piglets.

To study the effects of different feed additives on the weaning stress of Tibetan piglets, we selected 28 healthy, 30-day-old Tibetan weaned piglets and divided them into four groups, namely, the control group (basal feed without any antibiotic additions) (Nor), the group with the addition of the antibiotic lincomycin (Ant), the group with the addition of fifteen-flavor black pills of Tibetan medicine (Tib), and the group with the addition of fecal bacterial supernatant (Fec). We measured growth performance, blood physiological indexes, and metabolomics. The results showed that the Ant, Tib, and Fec groups significantly reduced the ratio of diarrhea to feed/weight (F/G) and increased the average daily gain (ADG) compared with the Nor group (p < 0.01). The Nor group had significantly lower leukocyte counts, hemoglobin levels, and erythrocyte counts compared with the other three groups at 21 d (p < 0.05). These physiological indexes tended to stabilize at 42 d. We found that there were beneficial metabolites and metabolic pathways for gastrointestinal function. Specifically, the porphyrin metabolic pathway was elevated in the Ant group, and the tryptophan metabolic pathway was significantly elevated in the Tib and Fec groups compared with the Nor group (p < 0.05). In conclusion, adding fecal bacterial supernatant and fifteen-flavor black pills of Tibetan medicine to the feed reduced the rate of diarrhea and improved the growth performance of the piglets. Moreover, it had an effect on the microorganisms and their metabolites and pathways in the gastrointestinal tract of the animals, which might be the main reason for influencing the diarrhea rate of weaned Tibetan piglets and the growth and development of the piglets. This study provides a new approach for anti-stress applications in weaned Tibetan piglets and the development of substitute anti-products.

Battery wastewater induces nephrotoxicity via disordering the mitochondrial dynamics.

The rapid development of new energy battery enterprises manifolds the obsolete and scrapped batteries which are considered serious concern for the environment and ecology. Increasing trend of recycling batteries waste is public hazard throughout the world. The batteries wastes affect the various body systems but exact toxicological mechanism of battery wastewater is still unexplored. The present study was designed to observe the toxicological effects of batteries wastes on kidney functional dynamics. In this experiment, a total of 20 male mice were randomly divided into two groups including control and treatment (battery wastewater) group. The control group was provided the normal saline while the battery wastewater group were provided battery waste-water for a period of 21 days. The isolated kidneys were processed for histopathological analysis, biochemical assays, mRNA and protein estimation. The results showed that battery wastewater provision increased the mitochondrial division-related genes and proteins (Drp1, MFF, Fis1) and decreased the expression level of fusion-related nuclear proteins (MFN1, MFN2, OPA1) in kidneys. Moreover, the battery wastewater exposure significantly up-regulated the autophagy (PINK, Parkin, mTOR, ATG5, LC3-b, p62) and apoptosis (Bax, Cytc, APAF1, P53, Caspase3, Caspase8) related mRNA and proteins levels in kidneys. However, down-regulation of mRNA and proteins levels of Bcl2 and Beclin1 were also observed in kidneys after batteries wastes exposure. In conclusion, it is evident that the battery wastewater leads to renal apoptosis and autophagy by disrupting the mitochondrial dynamics in mice kidneys.

Mitochondrial calcium uniporter involved in foodborne mycotoxin-induced hepatotoxicity.

Foodborne mycotoxins are toxic metabolites that are produced by fungi. The widespread contamination of food and its by-products by mycotoxins is a global food safety problem that potentially threatens public health and other exposed animals. Most foodborne mycotoxins induce hepatotoxicity. However, only few studies have investigated the regulatory mechanisms of mitochondrial calcium transport monomers in mycotoxin-induced hepatotoxicity. Therefore, according to relevant studies and reports, this review suggests that intracellular Ca(2 +) homeostasis and mitochondrial Ca(2 +) uniporter are involved in the regulation of mycotoxin-induced hepatotoxicity. This review provides some ideas for future research involving mitochondrial Ca(2 +) uniporter in the molecular targets of mycotoxin-induced hepatotoxicity, as well as a reference for the research and development of related drugs and the treatment of related diseases.

Reassessing the evolutionary history of ass-like equids: insights from patterns of genetic variation in contemporary extant populations.

All extant equid species are grouped in a single genus - Equus. Among those, ass-like equids have remained particularly unstudied and their phylogenetic relations were poorly understood, most probably because they inhabit extreme environments in remote geographic areas. To gain further insights into the evolutionary history of ass-like equids, we have used a non-invasive sampling approach to collect representative fecal samples of extant African and Asiatic ass-like equid populations across their distribution range and mitochondrial DNA (mtDNA) sequencing analyses to examine intraspecific genetic diversity and population structure, and to reconstruct phylogenetic relations among wild ass species/subspecies. Sequence analyses of 410 base pairs of the fast evolving mtDNA control region identified the Asiatic wild ass population of Kalamaili (China) as the one displaying the highest diversity among all wild ass populations. Phylogenetic analyses of complete cytochrome b sequences revealed that African and Asiatic wild asses shared a common ancestor approximately 2.3Mya and that diversification in both groups occurred much latter, probably driven by climatic events during the Pleistocene. Inferred genetic relationships among Asiatic wild ass species do not support E. kiang monophyly, highlighting the need of more extensive studies in order to clarify the taxonomic status of species/subspecies belonging to this branch of the Equus phylogeny. These results highlight the importance of re-assessing the evolutionary history of ass-like equid species, and urge to extend studies at the population level to efficiently design conservation and management actions for these threatened species.