RESUMEN
Seven undescribed C27 steroidal glycosides, Seladelicatulasine A-G, including six cholestanol glycosides and one spirostanol glycoside, were isolated from Selaginella delicatula. Their structures were elucidated by 1D/2D NMR spectra and HRESIMS analyses. The absolute configurations of the sugars were determined by enzymatic hydrolysis and GC/MS analyses. These cholestanol glycosides were isolated from the family Selaginellaceae for the first time. Seladelicatulasine F is characterized as a rare B-5,6-secosteroid. In addition, all the compounds were evaluated for their inhibitory activities against cholinesterase (AChE/BChE) and monoamine oxidase (MAO-A/MAO-B). These steroidal glycosides displayed selective inhibition activities on cholinesterase. Seladelicatulasine A, B and E inhibited the AChE activity with IC50 values of 0.31, 0.09, and 0.04 µM, respectively. Seladelicatulasine A and F showed the strongest inhibition activity on BChE with IC50 values of 0.37 and 0.65 µM, respectively.
Asunto(s)
Selaginellaceae , Inhibidores de la Colinesterasa/farmacología , Colinesterasas , Glicósidos/farmacología , Estructura Molecular , MonoaminooxidasaRESUMEN
Three new neolignan derivatives (1-3), together with three known isolariciresinol derivatives (4-6) were isolated from Selaginella picta. Their structures were elucidated by spectroscopic methods (1D/2D NMR, HRESIMS and CD). All isolated compounds were assayed on the neuroprotective activity against the injury of HT-22 cells induced by L-Glutamate in vitro. All compounds displayed potent protective effect on HT-22 cells.
Asunto(s)
Lignanos/química , Fármacos Neuroprotectores/química , Selaginellaceae/química , Animales , Evaluación Preclínica de Medicamentos , Ácido Glutámico/toxicidad , Lignanos/farmacología , Espectroscopía de Resonancia Magnética , Ratones , Estructura Molecular , Fármacos Neuroprotectores/farmacología , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
Phytochemical study on the n-BuOH extract of Selaginella delicatula lead to the isolation, characterization and structure elucidation of two new adenine analogues, delicatulines A (1) and B (2), one new pyrrole alkaloid (4), and five known compounds (3, 5-8). These new substances all contain an aliphatic chain in their parent nucleus, which were unusual to find in plants. In the present study, they were identified from Selaginellaceae for the first time. The structures and absolute configurations of these new compounds were determined by a combination of NMR and CD spectroscopic analyses. Compounds 1, 3 and 4 were evaluated for their inhibitory activities on HBV surface antigen and HBV DNA in HepAD38 cells. The results showed that these compounds had only weak or no inhibitive effects on HBV.
Asunto(s)
Adenina/análogos & derivados , Pirroles/aislamiento & purificación , Selaginellaceae/química , Adenina/aislamiento & purificación , Alcaloides/aislamiento & purificación , Antígenos Virales/efectos de los fármacos , Antivirales/aislamiento & purificación , Antivirales/farmacología , Dicroismo Circular , ADN Viral/efectos de los fármacos , Virus de la Hepatitis B , Humanos , Espectroscopía de Resonancia Magnética , Estructura MolecularRESUMEN
A new neolignan, selamoellenin A (1), was isolated from the whole plants of Selaginella moellendorffii Hieron. The structure was elucidated on the basis of comprehensive spectroscopic methods (1D/2D NMR and HRMS). Compound 1 was evaluated for its protective effect against high glucose-induced human umbilical vein endothelial cells (HUVECs) damage in vitro.
