Antimicrobial and quorum sensing inhibitory activity of epiphytic bacteria isolated from the red alga Halymenia durvillei.

Halymenia durvillei is a red alga that is commonly utilized in the Philippines as food and as a source of high-value natural products for industrial applications. However, there are no studies regarding the microbial community associated with H. durvillei and its potential applications. This study aimed to isolate and identify the epiphytic bacteria of H. durvillei and determine their antimicrobial and quorum sensing inhibitory (QSI) effects. The thalli of H. durvillei were collected at the shores of Santa Fe, Bantayan, Cebu, Philippines. Bacterial isolates were identified using 16S rRNA, and their ethyl acetate (EtOAc) extracts were subjected to antimicrobial susceptibility tests against representative species of yeast and Gram-negative and Gram-positive bacteria. Their QSI activity against Chromobacterium violaceum was also determined. Fourteen distinct bacterial colonies belonging to four genera, namely Alteromonas (3), Bacillus (5), Oceanobacillus (1) and Vibrio (5), were successfully isolated and identified. All 14 bacterial isolates exhibited antibacterial effects. EPB9, identified as Bacillus safensis , consistently showed the strongest inhibition against Escherichia coli , Staphylococcus aureus and Staphylococcus epidermidis , with minimum inhibitory concentrations (MICs) ranging from 0.0625 to 1.0 mg ml-1. In contrast, all 14 isolates showed weak antifungal effects. Both B. safensis (EPB9) and Bacillus australimaris (EPB15) exhibited QSI effects at 100 mg ml-1, showing opaque zones of 3.1±0.9 and 3.8±0.4 mm, respectively. This study is the first to isolate and identify the distinct microbial epiphytic bacterial community of H. durvillei and its potential as an abundant resource for new antibacterial and QSI bioactives.

Bioactivity of Amaranthus spinosus L. leaf extracts and meals against Aeromonas hydrophila.

Plant-based protein is being sought after as a substitute for fish meals (powdered fish) in tilapia feeds. This is to promote sustainable aquaculture, as fish meals contribute to the dwindling marine fish catch. Amaranthus spinosus is an edible weed that shows potential to improve the growth and immunity of Nile tilapia. However, most studies only consider the survivability of fish to evaluate the benefit of using plant-based feeds and do not necessarily elucidate whether a pathogen is affected in vivo. A. spinosus leaf meals (ASLMs) were used to determine effectiveness against Aeromonas hydrophila (BIOTECH 10089) injected intraperitoneally into Nile tilapia. Formulated feeds with fish meals substituted with 50 % (ASLM50) and 75 % (ASLM75) A. spinosus leaves were fed to Nile tilapia challenged with A. hydrophila . Then spleen and kidney tissue were collected and analysed 10 days post-injection for total plate count. The fish fed with ASLM50 appeared healthier than those fed with ASLM75 and those fed with control feeds. Fish fed with ASLMs had lower A. hydrophila counts (P=0.03). Phytochemical screening and antimicrobial activity determination for crude methanolic A. spinosus leaf (ASL) and ASLMs were also conducted to enhance the in vivo results. The metabolites present in the extracts were carbohydrates, amino acids and proteins, cardiac glycosides, saponins and terpenoids. The ASL and ASLM extracts had antimicrobial activity (MIC=115 mg ml-1). Overall, the study showed that ASLMs can make tilapia more resilient against A. hydrophila infections. Fish meal substitution was best at 50 %. Higher substitution had unwanted effects (more bacterial counts), possibly due to antinutritional factors.

Microstructure and molecular vibration of mannosylerythritol lipids from Pseudozyma yeast strains.

This work highlights microstructure and molecular vibration of mannosylerythritol lipids (MELs) from Pseudozyma aphidis B1 and Pseudozyma hubeiensis TS18 strains collected from brown algae and mangrove sediments. The scanning electron microscopy (SEM) shows the elongated structures with polar budding in the cells of B1 and TS18 yeast strains. The high-resolution transmission electron microscopy (HRTEM) identifies large lipid bodies that contain MELs confirmed by the anthrone test and thin layer chromatography. The HRTEM also reveals unknown electron dense inclusions. The surface-enhanced Raman scattering (SERS) was used to analysis molecular vibrations of cells, MEL mixtures, and purified MELs (A, B, and C) extracted from the B1 and TS18 cells. The peak analysis of Raman spectra suggests a higher level of saturation per fatty acid chain in MEL-B in both B1 and TS18 cells. This work demonstrates that the out-of-plane bending vibrations of the CH bonds in the range of 840-940 cm-1 can serve an efficient indicator for detecting MEL-A, -B, and -C.