RESUMEN
BACKGROUND: Mast cells synthesize and secrete chemical mediators which play a central role in anaphylaxis. METHODS: The effect of Acanthopanax senticosus root (ASR) on mast cell-dependent anaphylaxis was investigated. RESULTS: ASR inhibited compound 48/80-induced systemic anaphylactic shock at the dose of 1.0 g/kg by 50%. When ASR was given as pre-treatment at concentrations ranging from 0.01 to 2.0 g/l, the histamine release from rat peritoneal mast cells induced by compound 48/80 was reduced in a dose-dependent manner. ASR (2.0 g/kg) also inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl (DNP) IgE to 53.17+/-6.62%. Moreover, ASR inhibited tumor necrosis factor-alpha production in a concentration-dependent manner, and the treatment of 1 g/l blocked the production by 32.5+/-3.50% compared to saline value. CONCLUSIONS: ASR may possess effective anti-anaphylactic activity.
Asunto(s)
Anafilaxia/tratamiento farmacológico , Mastocitos/efectos de los fármacos , Extractos Vegetales/farmacología , Anafilaxia/inducido químicamente , Anafilaxia/patología , Animales , Células Cultivadas , Dinitrobencenos/inmunología , Histamina/metabolismo , Inmunoglobulina E/farmacología , Masculino , Mastocitos/metabolismo , Ratones , Ratones Endogámicos ICR , Raíces de Plantas/química , Plantas Medicinales , Ratas , Ratas Sprague-Dawley , Factor de Necrosis Tumoral alfa/metabolismo , p-Metoxi-N-metilfenetilamina/efectos adversosRESUMEN
The metabolic pathway of chiisanoside isolated from leaves of Acanthopanax divaricatus var. albeofructus (Araliaceae) by human intestinal bacteria and by the protein fraction of leaves of this plant were investigated, and the cytotoxic and anti-rotaviral activities of chiisanoside and its metabolite, chiisanogenin, were assayed. Chiisanogenin was produced as a main metabolite, when chiisanoside were incubated for 15 h with human intestinal bacteria. This metabolic pathway proceeded more potently with the protein fraction than with human intestinal bacteria. The in vitro cytotoxicity of chiisanogenin was superior to that of chiisanoside. H+/K+ ATPase was more potently inhibited by chiisanogenin than by chiisanoside. However, the anti-rotaviral activity of chiisanoside was more potent than that of chiisanogenin.
Asunto(s)
Antineoplásicos Fitogénicos/metabolismo , Antivirales/metabolismo , Bacterias/metabolismo , Intestinos/microbiología , Plantas Medicinales , Triterpenos/metabolismo , Animales , Humanos , Masculino , Ratas , Ratas Sprague-DawleyRESUMEN
Two 3,4-seco-lupane triterpenoids (1, 2) were isolated from the leaves of Acanthopanax divaricatus var. albeofructus (Araliaceae). Based on the spectroscopic data, the chemical structures of 1 and 2 were characterized as 24-hydroxychiisanogenin and 22alpha-hydroxychiisanogenin, respectively. 1 was a new compound and 2 was isolated for the first time from the natural source, although it had been obtained as an enzymatically hydrolyzed artifact from 22alpha-hydroxychiisanoside.
Asunto(s)
Triterpenos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Estructura Molecular , Hojas de la Planta/química , Triterpenos/químicaRESUMEN
Four new 3,4-seco-lupane-type triterpene glycosides (1-4) were isolated from the leaves of Acanthopanax senticosus forma inermis. The structures of 1-4 were established as 11alpha-hydroxy-3, 4-seco-lup-4(23),20(30)-dien-3-oic acid methyl ester 28-oic acid 28-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-be ta-D-glucopyranoside, designated as inermoside (1); 1-deoxychiisanoside (2); 24-hydroxychiisanoside (3); and 11-deoxyisochiisanoside (4) by (1)H-(1)H COSY and (1)H-(13)C COSY(HMBC, HMQC) methods and FABMS.
Asunto(s)
Glicósidos/aislamiento & purificación , Magnoliopsida/química , Triterpenos/aislamiento & purificación , Conformación de Carbohidratos , Secuencia de Carbohidratos , Glicósidos/química , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Plantas Medicinales/química , Triterpenos/químicaRESUMEN
This report contains the first characterization of acanthodiolglycoside which belongs to pentacyclic lupane triterpene glycoside.
