RESUMEN
The physiological mechanisms underlying Stage II transport (STII), during which comminuted solid food is transported from the oral cavity into the meso-pharynx for aggregation into a pre-swallow bolus, have yet to be clarified. The purpose of the present study was to investigate relationships between tongue-palate contact during mastication and incidence of STII by synchronised analysis of tongue pressure production on a hard palate and video-endoscopic (VE) images during mastication. Tongue pressure at 5 measuring points with an ultra-thin sensor sheet attached to the hard palate and trans-nasal VE images while masticating corned beef was recorded for 12 healthy subjects. All recordings were divided into 2 groups: mastication with STII and without STII. Tongue pressure duration was longer at the anterior-median part in the group with STII than in the group without STII. Integrated values of tongue pressure were greater at the anterior-median parts and posterior circumferential part in the group with STII. Integrated values of tongue pressure per second were greater in late-stage mastication than in early-stage mastication in the group with STII. These results suggest that the tongue-palate contacting at the anterior-median and post-circumferential parts of the hard palate is related with the incidence of STII.
Asunto(s)
Deglución/fisiología , Masticación/fisiología , Paladar Duro/fisiología , Lengua/fisiología , Adulto , Fenómenos Biomecánicos , Femenino , Alimentos , Voluntarios Sanos , Humanos , Masculino , Paladar Duro/anatomía & histología , Faringe/fisiología , Presión , Lengua/anatomía & histología , Grabación en Video , Adulto JovenRESUMEN
Idiopathic pulmonary fibrosis (IPF) is a fatal disease with a median survival of 3-4 years after diagnosis. It is the most frequent form of a group of interstitial pneumonias of unknown etiology. Current available therapies prevent deterioration of lung function but no therapy has shown to improve survival. Periostin is a matricellular protein of the fasciclin 1 family. There is increased deposition of periostin in lung fibrotic tissues. Here we evaluated whether small interfering RNA or antisense oligonucleotide against periostin inhibits lung fibrosis by direct administration into the lung by intranasal route. Pulmonary fibrosis was induced with bleomycin and RNA therapeutics was administered during both acute and chronic phases of the disease. The levels of periostin and transforming growth factor-ß1 in airway fluid and lung tissue, the deposition of collagen in lung tissue and the lung fibrosis score were significantly reduced in mice treated with siRNA and antisense against periostin compared to control mice. These findings suggest that direct administration of siRNA or antisense oligonucleotides against periostin into the lungs is a promising alternative therapeutic approach for the management of pulmonary fibrosis.
Asunto(s)
Moléculas de Adhesión Celular/genética , Moléculas de Adhesión Celular/fisiología , Fibrosis Pulmonar/terapia , Administración Intranasal/métodos , Animales , Bleomicina/farmacología , Colágeno/análisis , Femenino , Fibroblastos/metabolismo , Fibrosis , Fibrosis Pulmonar Idiopática/genética , Fibrosis Pulmonar Idiopática/metabolismo , Fibrosis Pulmonar Idiopática/terapia , Pulmón/metabolismo , Ratones , Ratones Endogámicos C57BL , Oligonucleótidos , Oligonucleótidos Antisentido/metabolismo , Fibrosis Pulmonar/genética , Fibrosis Pulmonar/metabolismo , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/metabolismo , ARN Interferente Pequeño/farmacología , Factor de Crecimiento Transformador beta/análisisRESUMEN
ß-Conglycinin, a major component of seed storage protein in soybean, comprises three subunits: α, α' and ß. The expression of genes for these subunits is strictly controlled during embryogenesis. The proximal promoter region up to 245 bp upstream of the transcription start site of the α subunit gene sufficiently confers spatial and temporal control of transcription in embryos. Here, the binding profile of nuclear proteins in the proximal promoter region of the α subunit gene was analysed. DNase I footprinting analysis indicated binding of proteins to the RY element and DNA regions including box I, a region conserved in cognate gene promoters. An electrophoretic mobility shift assay (EMSA) using different portions of box I as a probe revealed that multiple portions of box I bind to nuclear proteins. In addition, an EMSA using nuclear proteins extracted from embryos at different developmental stages indicated that the levels of major DNA-protein complexes on box I increased during embryo maturation. These results are consistent with the notion that box I is important for the transcriptional control of seed storage protein genes. Furthermore, the present data suggest that nuclear proteins bind to novel motifs in box I including 5'-TCAATT-3' rather than to predicted cis-regulatory elements.
Asunto(s)
Antígenos de Plantas/genética , Regulación de la Expresión Génica de las Plantas , Globulinas/genética , Glycine max/genética , Proteínas de Almacenamiento de Semillas/genética , Proteínas de Soja/genética , Antígenos de Plantas/metabolismo , Secuencia de Bases , Cotiledón/metabolismo , Huella de ADN , Ensayo de Cambio de Movilidad Electroforética , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Globulinas/metabolismo , Glucuronidasa/metabolismo , Datos de Secuencia Molecular , Proteínas Nucleares , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas/genética , Unión Proteica , Subunidades de Proteína , Proteínas de Almacenamiento de Semillas/metabolismo , Análisis de Secuencia de ADN , Proteínas de Soja/metabolismo , Glycine max/embriología , Glycine max/metabolismoRESUMEN
A 12 × 12 pixel detector has been developed and used in a laboratory experiment for lost fast-ion diagnostics. With gamma rays in the MeV range originating from nuclear reactions (9)Be(α, nγ)(12)C, (9)Be(d, nγ)(12)C, and (12)C(d, pγ)(13)C, a high purity germanium (HPGe) detector measured a fine-energy-resolved spectrum of gamma rays. The HPGe detector enables the survey of background-gamma rays and Doppler-shifted photo peak shapes. In the experiments, the pixel detector produces a gamma-ray image reconstructed from the energy spectrum obtained from total photon counts of irradiation passing through the detector's lead collimator. From gamma-ray image, diagnostics are able to produce an analysis of the fast ion loss onto the first wall in principle.
RESUMEN
Reactive oxygen species (ROS) play a key role in neoplastic growth and tumor invasion is supported by various experimental data. In this study, we analyzed the participation of ROS in the RET tyrosine auto-phosphorylation. The NIH3T3 cell lines transfected with cRET, MEN2A, and MEN2B individually (designated NIH3T3cRET, NIH3T3 RET-MEN2A, and NIH3T3RET-MEN2B) showed the elevated levels of intracellular ROS, and concomitantly increased Rac1 expression, as well as down-regulation of Mn SOD and Cu/Zn SOD in comparison with the parental cell line expressing RET. H2O2 enhanced the constitutive tyrosine auto-phosphorylation of RET-MEN2A and RET-MEN2B proteins, and this increase was attenuated by treatment with the NOX inhibitor diphenyliodonium (DPI) or catalase. We also showed that DPI inhibited dimerization of RET-MEN2A. Elevated ROS derived from NOX1 activation and downregulation of SOD in NIH3T3RET-MEN2A and NIH3T3RET-MEN 2B cells may be involved in RET constitutive tyrosine auto-phosphorylation, and scavengers of ROS such as catalase and blocking NOX1 are useful for targeting RET tyrosine kinase activation in cancer.
Asunto(s)
NADH NADPH Oxidorreductasas/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Proteínas Tirosina Quinasas Receptoras/metabolismo , Animales , Proliferación Celular , Humanos , Ratones , NADH NADPH Oxidorreductasas/genética , NADPH Oxidasa 1 , Fosforilación , Proteínas Tirosina Quinasas Receptoras/genética , Transducción de SeñalRESUMEN
Kenyon cells, intrinsic neurons of the insect mushroom body, have been assumed to be a site of conditioning stimulus (CS) and unconditioned stimulus (US) association in olfactory learning and memory. Acetylcholine (ACh) has been implicated to be a neurotransmitter mediating CS reception in Kenyon cells, causing rapid membrane depolarization via nicotinic ACh receptors. However, the long-term effects of ACh on the membrane excitability of Kenyon cells are not fully understood. In this study, we examined the effects of ACh on Na(+) dependent action potentials (Na(+) spikes) elicited by depolarizing current injection and on net membrane currents under the voltage clamp condition in Kenyon cells isolated from the mushroom body of the cricket Gryllus bimaculatus. Current-clamp studies using amphotericin B perforated-patch recordings showed that freshly dispersed cricket Kenyon cells could produce repetitive Na(+) spikes in response to prolonged depolarizing current injection. Bath application of ACh increased both the instantaneous frequency and the amplitudes of Na(+) spikes. This excitatory action of ACh on Kenyon cells is attenuated by the pre-treatment of the cells with the muscarinic receptor antagonists, atropine and scopolamine, but not by the nicotinic receptor antagonist mecamylamine. Voltage-clamp studies further showed that bath application of ACh caused an increase in net inward currents that are sensitive to TTX, whereas outward currents were decreased by this treatment. These results indicate that in order to mediate CS, ACh may modulate the firing properties of Na(+) spikes of Kenyon cells through muscarinic receptor activation, thus increasing Na conductance and decreasing K conductance.
Asunto(s)
Acetilcolina/farmacología , Gryllidae/fisiología , Cuerpos Pedunculados/fisiología , Neuronas/fisiología , Acetilcolina/antagonistas & inhibidores , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/fisiología , Anfotericina B/farmacología , Animales , Atropina/farmacología , Encéfalo/citología , Encéfalo/efectos de los fármacos , Antagonistas Colinérgicos/farmacología , Gryllidae/citología , Masculino , Mecamilamina/farmacología , Cuerpos Pedunculados/citología , Cuerpos Pedunculados/efectos de los fármacos , Neuronas/efectos de los fármacos , Antagonistas Nicotínicos/farmacología , Técnicas de Placa-Clamp , Escopolamina/farmacologíaAsunto(s)
Perforación del Esófago/cirugía , Tejido de Granulación , Stents , Anciano , Humanos , MasculinoRESUMEN
Octopamine plays an important role in mediating reward signals in olfactory learning and memory formation in insect. However, its target molecules and signaling pathways are still unknown. In this study, we investigated the effects of octopamine on the voltage-activated Ca2+ channels expressed in native Kenyon cells isolated from the mushroom body of the cricket (Gryllus bimaculatus) brain. The cell-attached patch clamp recordings with 100 mM Ba2+ outside showed the presence of dihydropyridine (DHP) sensitive L-type Ca2+ channels with a single channel conductance of approximately 21+/-2 pS (n=12). The open probability (NPo) of single Ca2+ channel currents decreased by about 29+/-7% (n=6) by bath application of 10 microM octopamine. Octopamine-induced decrease in Po was imitated by bath application of 8-Br-cAMP, a membrane-permeable cAMP analog. Pre-treatment of Kenyon cells with the octopamine receptor antagonist phentolamine blocked the inhibitory effect of octopamine on Ca2+ channels. Pre-treatment of Kenyon cells with H-89, a selective inhibitor of cAMP-dependent protein kinase (PKA) attenuated the inhibitory effect of bath applied octopamine on Ca2+ channels. These results indicate that DHP-sensitive L-type Ca2+ channel is a target protein for octopamine and its modulation is mediated via cAMP and PKA-dependent signaling pathways in freshly isolated Kenyon cell in the cricket G. bimaculatus.
Asunto(s)
Canales de Calcio/metabolismo , Gryllidae/fisiología , Activación del Canal Iónico/fisiología , Cuerpos Pedunculados/citología , Octopamina/metabolismo , Animales , Encéfalo/fisiología , GMP Cíclico/análogos & derivados , Electrofisiología , Cuerpos Pedunculados/fisiologíaAsunto(s)
Adenocarcinoma/patología , Adenocarcinoma/cirugía , Neoplasias Duodenales/patología , Neoplasias Duodenales/cirugía , Mucosa Intestinal/cirugía , Anciano , Biopsia con Aguja , Duodenoscopía , Endoscopía/métodos , Estudios de Seguimiento , Humanos , Inmunohistoquímica , Mucosa Intestinal/patología , Japón , Masculino , Procedimientos Quirúrgicos Mínimamente Invasivos/métodos , Estadificación de Neoplasias , Medición de Riesgo , Resultado del TratamientoRESUMEN
The canal network in cortical bone is an indispensable basis of bone vascularity, and its structure changes according to bone growth. Using monochromatic synchrotron radiation microCT (SRmicroCT), we evaluated the structural change of the canal network in growing rat tibiae and the response of this network to disuse. Tibiae were harvested from both hindlimbs of 9- and 14-week-old male Wistar rats subjected to unilateral sciatic neurectomy (SN) at 6 weeks of age (W9, n=8; W14, n=8) and from intact hindlimbs of 6-week-old rats (W6, n=8). Images of distal diaphyseal segments were reconstructed by SRmicroCT with a voxel size of 5.83 mum and then translated into local mineral densities using a calibrated relation between linear absorption coefficients and the concentration of K(2)HPO(4) solution. The canal network was segmented by simple thresholding at a bone mineral density of 0.82 g.cm(-3) and its structural properties were determined. In intact hindlimbs, the canal network showed a biphasic change with growth, as represented by increases followed by decreases in canal volume fraction (Ca.vol.f), the density of canals running longitudinally (Ca.num.d), and the density of canal connections (Ca.con.d): Ca.vol.f=2.2, 3.1, and 1.8%, Ca.num.d=77, 98, and 70 mm(-2), and Ca.con.d=18, 41, and 21 mm(-3) in W6, W9, and W14, respectively. In SN hindlimbs, bone growth deceleration was accompanied by a 16% smaller Ca.vol.f and a 22% smaller Ca.con.d in W9 and a 27% smaller Ca.vol.f, a 12% smaller Ca.num.d, and a 39% smaller Ca.con.d in W14 than those in intact hindlimbs. Furthermore, the canal branching structure became more treelike in SN hindlimbs. The effect of SN on the canal network appeared mainly in the periosteal sector of the anteriolateral cortex in W9 and spread throughout the cortex in W14. These findings will lead to a better understanding of microcirculation in cortical bone growth.
Asunto(s)
Desarrollo Óseo/fisiología , Resorción Ósea , Tibia , Animales , Masculino , Ratas , Ratas Wistar , Sincrotrones , Tibia/anatomía & histología , Tibia/fisiología , Tomografía Computarizada por Rayos X/métodosRESUMEN
Prooxidant properties of furanone compounds including 2,5-furanone (furaneol, 4-hydroxy-2,5-dimethyl-furan-3-one), 4,5-furanone (4,5-dimethyl-3-hydroxy-2(5H)-furanone) (sotolone) and cyclotene (2-hydroxy-3-methyl-2-cyclopenten-1-one) were analyzed in relation to the metal-reducing activity. Only 2.5-furanone known as a "strawberry or pineapple furanone" inactivated aconitase the most sensitive enzyme to active oxygen in the presence of ferrous sulfate, suggesting the furaneol/iron-mediated generation of reactive oxygen species. 2,5-Furanone caused strand scission of pBR322 DNA in the presence of copper. Treatment of calf thymus DNA with 2,5-furanone plus copper produced 8-hydroxy-2'-deoxyguanosine in DNA. 2,5-Furanone showed a potent copper-reducing activity, and thus, DNA strand breaks and the formation of 8-hydroxy-2'-deoxyguanosine by 2,5-furanone can be initiated by the production of superoxide radical through the reduction of cupric ion to cuprous ion, resulting in the conversion to hydrogen peroxide and hydroxyl radical. However, an isomer and analog of 2,5-furanone, 4,5-furanone and cyclotene, respectively, did not show an inactivation of aconitase, DNA injuries including strand breakage and the formation of 8-hydroxy-2'-deoxyguanosine, and copper-reducing activity. Cytotoxic effect of 2,5-furanone with hydroxyketone structure can be explained by its prooxidant properties: furaneol/transition metal complex generates reactive oxygen species causing the inactivation of aconitase and the formation of DNA base damage by hydroxyl radical.
Asunto(s)
Cobre/farmacología , Roturas del ADN , Desoxiguanosina/análogos & derivados , Furanos/farmacología , Oxidantes/farmacología , Especies Reactivas de Oxígeno/metabolismo , 8-Hidroxi-2'-Desoxicoguanosina , Aconitato Hidratasa/antagonistas & inhibidores , Animales , Bovinos , Ciclopentanos/farmacología , Aductos de ADN/síntesis química , Desoxiguanosina/biosíntesis , Oxidación-Reducción/efectos de los fármacos , Especies Reactivas de Oxígeno/químicaRESUMEN
This study presents a technique developed for 3-D imaging and quantitative comparison of human dentitions and simulated bite marks. A sample of 42 study models and the corresponding bites, made by the same subjects in acrylic dental wax, were digitised by laser scanning. This technique allows image comparison of a 3-D dentition with a 3-D bite mark, eliminating distortion due to perspective as experienced in conventional photography. Cartesian co-ordinates of a series of landmarks were used to describe the dentitions and bite marks, and a matrix was created to compare all possible combinations of matches and non-matches using cross-validation techniques. An algorithm, which estimated the probability of a dentition matching its corresponding bite mark, was developed. A receiver operating characteristic graph illustrated the relationship between values for specificity and sensitivity. This graph also showed for this sample that 15% of non-matches could not be distinguished from the true match, translating to a 15% probability of falsely convicting an innocent person.
Asunto(s)
Mordeduras Humanas/patología , Dentición , Odontología Forense/métodos , Procesamiento de Imagen Asistido por Computador/métodos , Imagenología Tridimensional/métodos , Australia , Técnica de Impresión Dental , Humanos , Procesamiento de Imagen Asistido por Computador/instrumentación , Imagenología Tridimensional/instrumentación , Rayos Láser , Modelos Dentales , Odontometría , Curva ROC , Sensibilidad y EspecificidadRESUMEN
Protective role of NADP-isocitrate dehydrogenase in the oxidative inactivation of mitochondrial enzymes was analyzed. Administration of paraquat to the rat inactivated liver mitochondrial enzymes: the aconitase activity decreased to one quarter, and citrate synthase and fumarase to half, whereas cytosolic enzymes were not affected. Activities of heart mitochondrial and cytosolic enzymes were not at all changed in the rat treated with paraquat, but paraquat directly inactivated aconitase in the heart mitochondria isolated from the non-treated rats. The paraquat-dependent inactivation of aconitase was prevented by activating NADP-isocitrate dehydrogenase in the presence of oxidized glutathione. NADP-isocitrate dehydrogenase could regenerate glutathione in isolated heart mitochondria, indicating that paraquat-mediated inactivation depends on the glutathione-regenerating activity by enhanced NADPH supply. Lower NADP-isocitrate dehydrogenase activity in liver mitochondria cannot regenerate reduced glutathione for scavenging reactive oxygen species, resulting in the paraquat-induced oxidative inactivation of mitochondrial enzymes. However, higher activity of NADP-isocitrate dehydrogenase participates in the regeneration of reduced glutathione causing stabilization of enzymes in heart mitochondria.
RESUMEN
Stretch-activated channels (SACs) were investigated in myocytes isolated from the lateral oviduct in cricket Gryllus bimaculatus using the cell-attached or excised inside-out patch clamp technique. Application of both negative and positive pressure (10-100 cm H(2)O) into the patch pipettes induced the unitary channel current openings. The open probability (NPo) of the channel increased when negative pressure applied into the patch pipettes increased. The single channel conductance for this channel was approximately 20 pS with 140 mM Na(+), K(+), or Cs(+) in the patch pipettes and was approximately 13 pS with 100mM Ca(2+) or Ba(2+) in the patch pipettes. External application of Gd(3+), La(3+), Cd(2+) and Zn(2+)inhibited the channel with the IC(50) values of 14, 15, 28, and 18 microM respectively. Interestingly external application of TEA, a specific blocker of K(+) channel, also inhibited this channel with IC(50) value of 8.8mM. These results show for the first time the presence of stretch activated Ca(2+)-permeable nonselective cation channel in myocytes isolated from the cricket lateral oviduct. The physiological significance of this channel in oviposition behavior is discussed.
Asunto(s)
Canales de Calcio/fisiología , Calcio/metabolismo , Gryllidae/fisiología , Miocitos del Músculo Liso/fisiología , Oviductos/fisiología , Animales , Transporte Biológico Activo , Femenino , Oviductos/efectos de los fármacos , PresiónRESUMEN
The single Ca2+ channel activity was obtained from cell-attached patch recordings with the use of pipettes filled with 100 mM Ba2+ as the charge carrier in myocytes isolated from the lateral oviduct of cricket Gryllus bimaculatus. The following results were obtained. (1) The channel had a unitary conductance of 18 pS. (2) The open time histogram of the channel could be fitted with a single exponential while the closed time histogram could be fitted with the sum of two exponentials, suggesting that there are at least one open state and two closed states for this channel. (3) The open probability of the channel increased with increasing membrane depolarization. (4) The mean current reconstructed by averaging individual current trace responses inactivated slowly and the current-voltage relationship for the peak mean current showed a bell-shaped relation. (5) The dihydropyridine (DHP) Ca2+ antagonist, nifedipine, reduced the mean current by increasing the proportion of "blank" sweeps. On the other hand, the DHP Ca2+ agonist, Bay K 8644, increased the mean current by increasing the mean open-times of the channel. These results confirm a presence of DHP-sensitive L-type Ca2+ channel in myocytes isolated from the lateral oviduct of cricket G. bimaculatus.
Asunto(s)
Ácido 3-piridinacarboxílico, 1,4-dihidro-2,6-dimetil-5-nitro-4-(2-(trifluorometil)fenil)-, Éster Metílico/farmacología , Agonistas de los Canales de Calcio/farmacología , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio Tipo L/efectos de los fármacos , Células Musculares/metabolismo , Nifedipino/farmacología , Oviductos/metabolismo , Animales , Calcio/antagonistas & inhibidores , Calcio/farmacología , Canales de Calcio Tipo L/química , Canales de Calcio Tipo L/metabolismo , Femenino , Gryllidae , Técnicas In Vitro , Células Musculares/química , Oviductos/química , Técnicas de Placa-ClampRESUMEN
Competitive PCR assays were established for the mitochondrial DNA hypervariable region I and the human amelogenin locus. Using these assays, the copy numbers of DNA participating in PCR (amplifiable DNA) were quantified in tissues exposed to different environments. Human ribs, skin and nails were left in three exposure conditions (in the open air, in soil and in water). The amounts of amplifiable DNA in these tissues were quantified during a time period of up to two months. The amount of amplifiable DNA was well preserved in hard tissues (ribs and nails) regardless of the exposure conditions, whereas the soft tissues immersed in water showed a rapid decrease in amplifiable DNA. Strong PCR inhibition was observed in the DNA extracts obtained from buried bones. This phenomenon was clearly identified from an amplification failure of the internal standards in the competitive PCR. A preliminary examination to identify the PCR inhibitor suggested that the soil itself contributed to the inhibition. In addition, the amounts of amplifiable DNA in case samples were also investigated.
Asunto(s)
ADN/análisis , Ciencias Forenses/métodos , Reacción en Cadena de la Polimerasa/métodos , ADN Mitocondrial/análisis , Humanos , Técnicas de Amplificación de Ácido Nucleico/métodos , Especificidad de Órganos , Suelo , Factores de Tiempo , Conservación de Tejido/métodosRESUMEN
We have previously reported that the epidermal growth factor (EGF) family growth factor, epiregulin, is expressed in rat ovarian granulosa cells by induction with pregnant mare serum gonadotropin (PMSG). In this study, we report that amphiregulin, another member of the EGF family, was also induced in the rat ovary by gonadotropin treatment. Northern blot analysis revealed that PMSG treatment induced the expression of both epiregulin and amphiregulin mRNA after 24 h, but the expression then decreased 48 h after treatment. Further treatment with human chorionic gonadotropin (hCG) rapidly induced the expression of both epiregulin and amphiregulin genes and maximal levels were reached 4 h after hCG treatment. A marginal increase in amphiregulin mRNA levels was also observed 6 h after PMSG treatment. In situ hybridization revealed that epiregulin and amphiregulin mRNAs were localized in the granulosa cells of large antral follicles. These spatio-temporal expression patterns were similar to those of cyclo-oxygenase-2 (COX-2) and progesterone receptor (PR). In adult cycling rats, epiregulin and amphiregulin were strongly induced at 1800 and 2000 h on proestrus coinciding with the preovulatory LH surge. An in situ hybridization study also showed that epiregulin and amphiregulin mRNAs were detectable in the granulosa cells of preovulatory ovarian follicles at 2000 h on proestrus, where transcripts of COX-2 and PR were co-localized with those of epiregulin and amphiregulin. These observations suggested that the EGF family members, epiregulin and amphiregulin, may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats.
Asunto(s)
Factor de Crecimiento Epidérmico/genética , Glicoproteínas/genética , Péptidos y Proteínas de Señalización Intercelular/genética , Ovario/metabolismo , Anfirregulina , Animales , Northern Blotting , Familia de Proteínas EGF , Epirregulina , Femenino , Hibridación in Situ , Hormona Luteinizante/metabolismo , ARN Mensajero/genética , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Quantitative analysis of facial volume is important in the assessment of facial swelling. Previous research has demonstrated that the Fiore trade mark light-structured 3-D range camera is a rapid, non-invasive, and safe imaging technology for the quantitative analysis of facial morphology. The development of the Rugle-3D trade mark volumetric measurement software programme has allowed for the development of a powerful imaging and measurement system. This study aimed to determine the accuracy and reproducibility of Fiore trade mark by measuring simulated facial swelling. A model involving 20 human subjects was devised to simulate facial swelling for image acquisition and measurement in order to assess the accuracy and reproducibility of the device. A systematic error of 1.25% and reproducibility of 3.27% was determined for the imaging system. It is concluded that Fiore trade mark is an accurate and reproducible technology that is sensitive and would be applicable to clinical studies of facial swelling.
Asunto(s)
Edema/patología , Cara/patología , Imagenología Tridimensional/instrumentación , Fotografía Dental/instrumentación , Humanos , Maniquíes , Reproducibilidad de los Resultados , Procesamiento de Señales Asistido por ComputadorRESUMEN
The Ca2+-conducting pathway of myocytes isolated from the cricket lateral oviduct was investigated by means of the whole-cell patch clamp technique. In voltage-clamp configuration, two types of whole cell inward currents were identified. One was voltage-dependent, initially activated at -40 mV and reaching a maximum at 10 mV with the use of 140 mM Cs2+-aspartate in the patch pipette and normal saline in the bath solution. Replacement of the external Ca2+ with Ba2+ slowed the current decay. Increasing the external Ca2+ or Ba2+ concentration increased the amplitude of the inward current and the current-voltage (I-V) relationship was shifted as expected from a screening effect on negative surface charges. The inward current could be carried by Na+ in the absence of extracellular Ca2+. Current carried by Na+ (INa) was almost completely blocked by the dihydropyridine Ca2+ channel antagonist, nifedipine, suggesting that the INa is through voltage-dependent L-type Ca2+ channels. The other inward current is voltage-independent and its I-V relationship was linear between -100 mV to 0 mV with a slight inward rectification at more hyperpolarizing membrane potentials when 140 mM Cs+-aspartate and 140 mM Na+-gluconate were used in the patch pipette and in the bath solution, respectively. A similar current was observed even when the external Na+ was replaced with an equimolar amount of K+ or Cs+, or 50 mM Ca2+ or Ba2+. When the osmolarity of the bath solution was reduced by removing mannitol from the bath solution, the inward current became larger at negative potentials. The I-V relationship for the current evoked by the hypotonic solution also showed a linear relationship between -100 mV to 0 mV. Bath application of Gd3+ (10 microM) decreased the inward current activated by membrane hyperpolarization. These results clearly indicate that the majority of current activated by a membrane hyperpolarization is through a stretch-activated Ca2+-permeable nonselective cation channel (NSCC). Here, for the first time, we have identified voltage-dependent L-type Ca2+ channel and stretch-activated Ca2+-permeable NSCCs from enzymatically isolated muscle cells of the cricket using the whole-cell patch clamp recording technique.