RESUMEN
The incorporation of cationic groups onto electron-poor compounds is a viable strategy for achieving potent electron acceptors, as evidenced by reports of air-stable radical forms of large aromatic diimides such as naphthalene and perylene diimides. These ions have also been observed to exhibit anion-π interaction tendencies of interest in molecular recognition applications. The benefits of phosphonium incorporation, however, have not yet been extended to the smallest benzene diimides. Here, we report that dibrominated pyromellitic diimide and mellophanic diimide both readily undergo substitution reactions with phosphine sources to yield bisphosphonium compounds. In the single crystalline form, these dications display anion-π interactions and, in the case of mellophanic diimide, the stabilization of a bromide-water H-bonding ring pattern. The reaction of these dications with chemical reductants readily provides the singly and doubly reduced redox states, which were characterized by UV-vis spectroscopy and found to exhibit intense absorptions extending into the near-IR region. Taken together, this work demonstrates that phosphonium incorporation onto congested aromatic diimide scaffolds is synthetically viable and produces unusual electron-poor compounds.
RESUMEN
Reactions of (tricyclohexylphosphane)copper(I) chloride with two equivalents of potassium anthracene (KAn) in tetrahydrofuran (THF) at 200â K provides air-sensitive but thermally stable (at 293â K) solutions from which yellow crystalline blocks of bis[bis(tetrahydrofuran-κO)potassium] bis(µ-anthracene-κ2C9:C10)dicopper, [K(THF)2]2[{Cu(9,10-η2-C14H10)}2] or [K(C4H8O)2]2[Cu2(C14H10)2], 1, were isolated in about 50% yield. Single-crystal X-ray crystallographic analysis of 1 confirmed the presence of the first known (arene)cuprate. Also, unlike all previously known homoleptic (anthracene)metallates of d-block elements, which contain metals coordinated only to terminal rings, the organocuprate unit in 1 contains copper bound to the 9,10-carbons of the central ring of anthracene. No other d- or f-block metal is known to afford an anthracene or other aromatic hydrocarbon complex having the architecture of organodicuprate 1.
RESUMEN
Two new compounds, 1,1,1-tri(thioacetyl)ethane and 1,1-di(thioacetyl)ethene, arose during reactions of acetyl methoxy(thiocarbonyl) sulfide with potassium methyl xanthate. Relevant mechanisms were elucidated, which in turn suggested novel streamlined routes to these same compounds. Several further transformations of the title compounds were demonstrated, suggesting their potential synthetic utility.
RESUMEN
The importance of electron deficient Tp ligands motivates the introduction of electron-withdrawing substituents into the scorpionate framework. Since perfluorophenyltris(pyrazol-1-yl)borate affects significant anodic shifts in half-cell potentials in their metal complexes relative those of phenyltris(pyrazol-1-yl)borate analogues, the tuning opportunities achieved using 3,4,5-trifluorophenyl- and 3,5-bis(trifluoromethyl)phenyl(pyrazol-1-yl)borates were explored. Bis(amino)boranes ((3,4,5-F)C6H2)B(NMe2)2 and ((3,5-CF3)C6H3)B(NMe2)2 are precursors to fluorinated tris(pyrazol-1-yl)phenylborates. Thallium salts of these scorpionates exhibit bridging asymmetric κ3-N,N,N coordination modes consistent with the reduced π-basicity of the fluorinated phenyl substituents relative those of other structurally characterized tris(pyrazol-1-yl)phenylborates. While a comparative analysis of the spectral and X-ray crystallographic data for classical Mo(0), Mo(II), Mn(I), Fe(II) and Cu(II) complexes of [((3,4,5-F)C6H2)Bpz3]- and [((3,5-CF3)C6H3)Bpz3]- could not differentiate these ligands with respect to their metal-based electronic impacts, cyclic voltammetry suggests that 3,4,5-trifluorophenyl- and 3,5-bis(trifluoromethyl)phenyl(pyrazol-1-yl)borates affect similar anodic shifts within their metal complexes, with coordination of [((3,5-CF3)C6H3)Bpz3]- rendering metal centers more difficult to oxidize, and sometimes even more difficult to oxidize than their [C6F5Bpz3]- analogues. These data suggest that the extent of phenyl substituent fluorination necessary to minimize metal center electron-richness in phenyltris(pyrazol-1-yl)borate complexes cannot be confidently predicted.
RESUMEN
Four series of isostructural derivatives of 3-ring liquid crystalline derivatives containing p-carboranes (12-vertex A, and 10-vertex B), bicyclo[2.2.2]octane (C), or benzene (D) as the variable structural element were investigated for their mesogenic behavior and electronic interactions. Comparative studies demonstrated that the effectiveness of elements A-D in stabilization of the mesophase typically increases in the order: B
RESUMEN
Odd-electron bonds have unique electronic structures and are often encountered as transiently stable, homonuclear species. In this study, a pair of copper complexes supported by Group 13 metalloligands, M[N((o-C6H4)NCH2PiPr2)3] (M = Al or Ga), featuring two-center/one-electron (2c/1e) σ-bonds were synthesized by one-electron reduction of the corresponding Cu(i) ⢠M(III) counterparts. The copper bimetallic complexes were investigated by X-ray diffraction, cyclic voltammetry, electron paramagnetic spectroscopy, and density functional theory calculations. The combined experimental and theoretical data corroborate that the unpaired spin is delocalized across Cu, M, and ancillary atoms, and the singly occupied molecular orbital (SOMO) corresponds to a σ-(Cu-M) bond involving the Cu 4pz and M ns/npz atomic orbitals. Collectively, the data suggest the covalent nature of these interactions, which represent the first examples of odd-electron σ-bonds for the heavier Group 13 elements Al and Ga.
RESUMEN
Reduction of bis(naphthalene)vanadium(0) by potassium naphthalene (KNp) in tetrahydrofuran (THF) provides a highly reactive, thermolabile, and so far unisolable brown substance, which affords the first reported derivatives of bis(naphthalene)vanadates. From these solutions, thermally stable (298â K) and structurally characterized compounds have been obtained, including dark-red rods of catena-poly[bis(µ3-η4:η6:η4-naphthalene)tetrakis(tetrahydrofuran)dipotassiumvanadium], [K2V(C4H8O)4(C10H8)2]n or [K(THF)2]2[V(C10H8)2] (3), and red plates of (4,7,13,16,21,24-hexaoxa-1,10-diazabicyclo[8.8.8]hexacosane)potassium [1,2-bis(dimethylphosphanyl)ethane]bis(η4-naphthalene)vanadium tetrahydrofuran monosolvate, [K(C18H36N2O6)][V(C10H8)2(C6H16P2)]·C4H8O or [K([2.2.2]cryptand)][V(C10H8)2(dmpe)]·THF [dmpe is 1,2-bis(dimethylphosphanyl)ethane] (4b). Notably, [V(C10H8)2]2- is the only example of a structurally authenticated homoleptic bis(arene)metallate dianion and was obtained by further reduction of the brown material by KNp in THF, in the presence of trimethylphosphane (PMe3). Addition of anthracene (An) to the brown material in THF afforded deep-violet and paramagnetic crystalline (1,4,7,10,13,16-hexaoxacyclooctadecane)bis(tetrahydrofuran)potassium [(η4-anthracene)(tetrahydrofuran)vanadium]-µ-η4:η2-anthracene-[(1,4,7,10,13,16-hexaoxacyclooctadecane)potassium]-µ-η2:η4-anthracene-[(η4-anthracene)(tetrahydrofuran)vanadium] tetrahydrofuran disolvate, [K(C12H24O6)(C4H8O)2][KV2(C12H24O6)(C4H8O)2(C14H10)4]·2C4H8O or [K(18-crown-6)][K(18-crown-6)(THF)2][V(C14H10)2(THF)]2·2(THF) (5), which readily reacted with PMe3 and dmpe to give new vanadate salts. These were structurally characterized as (1,4,7,10,13,16-hexaoxacyclooctadecane)bis(tetrahydrofuran)potassium bis(η4-anthracene)(trimethylphosphane)vanadium tetrahydrofuran monosolvate, [K(C12H24O6)(C4H8O)2][V(C14H10)2(C3H9P)]·C4H8O or [K(18-crown-6)(THF)2][V(C14H10)2(PMe3)]·THF (6), and tetrakis(1,2-dimethoxyethane)potassium bis(η4-anthracene)[1,2-bis(dimethylphosphanyl)ethane]vanadium, [K(C4H10O2)4][V(C14H10)2(C6H16P2)] or [K(DME)4][V(C14H10)2(dmpe)] (DME is 1,2-dimethoxyethane) (7b). The last three structures contain the first known bis(anthracene)vanadates and are thereby derivatives of the unknown bis(anthracene)vanadium(0). Attempts to obtain the sodium salt analog of 5 in THF resulted instead in the formation of a unique substance, (µ3-η6:η6:η6-anthracene)(µ2-η6:η6-anthracene)tris(tetrahydrofuran)sodiumdivanadium, [NaV2(C14H10)2(C4H8O)3] or [Na(THF)3][V2(C14H10)2] (8), containing the first reported dimetallabis(anthracene) sandwich compound.
RESUMEN
Spin-vibronic coupling leads to spin relaxation in paramagnetic molecules, and an understanding of factors that contribute to this phenomenon is essential for designing next-generation spintronics technology, including single-molecule magnets and spin-based qubits, wherein long-lifetime magnetic ground states are desired. We report spectroscopic and magnetic characterization of the isoelectronic and isostructural series of homoleptic zerovalent transition metal triad M(CNDipp)6 (M = V, Nb, Ta; CNDipp = 2,6-diisopropylphenyl isocyanide) and show experimentally the significant increase in spin relaxation rate upon going from V to Nb to Ta. Correlated electronic calculations and first principle spin-phonon computations support the role of spin-orbit coupling in modulating spin-phonon relaxation. Our results provide experimental evidence that increasing magnetic anisotropy through spin-orbit coupling interactions leads to increased spin-vibronic relaxation, which is detrimental to long spin lifetime in paramagnetic molecules.
RESUMEN
In this study, a methyl group on the classic tetramethylcyclam (TMC) ligand framework is replaced with a benzylic group to form the metastable [FeIV (Osyn )(Bn3MC)]2+ (2-syn; Bn3MC=1-benzyl-4,8,11-trimethyl-1,4,8,11-tetraazacyclotetradecane) species at -40 °C. The decay of 2-syn with time at 25 °C allows the unprecedented monitoring of the steps involved in the intramolecular hydroxylation of the ligand phenyl ring to form the major FeIII -OAr product 3. At the same time, the FeII (Bn3MC)2+ (1) precursor to 2-syn is re-generated in a 1:2 molar ratio relative to 3, accounting for the first time for all the electrons involved and all the Fe species derived from 2-syn as shown in the following balanced equation: 3 [FeIV (O)(LPh )]2+ (2-syn)â2 [FeIII (LOAr )]2+ (3)+[FeII (LPh )]2+ (1)+H2 O. This system thus serves as a paradigm for aryl hydroxylation by FeIV =O oxidants described thus far. It is also observed that 2-syn can be intercepted by certain hydrocarbon substrates, thereby providing a means to assess the relative energetics of aliphatic and aromatic C-H hydroxylation in this system.
Asunto(s)
Hidrocarburos Aromáticos/química , Compuestos de Hierro/síntesis química , Oxígeno/química , Hidroxilación , Compuestos de Hierro/química , Estructura MolecularRESUMEN
Levothyroxine sodium pentahydrate (LSP; C15H10I4NNaO4·5H2O) gradually loses one molecule of water of crystallization as the water vapor pressure is decreased from 90% to 15% RH (40 °C), a behavior characteristic of nonstoichiometric hydrates. LSP loses four molecules of water of crystallization to form levothyroxine sodium monohydrate (LSM; C15H10I4NNaO4·H2O) under realistic storage conditions (40 °C/0% RH for 3 h). The crystal structure of LSP was determined following which the specimen was partially dehydrated in situ to form LSM. The crystal structure of LSM provided insight into its potential for high reactivity. Thus, its presence in a drug product is undesirable. In LSP-oxalic acid mixtures stored in a hermetic container at 40 °C, there was moisture transfer from drug to excipient. Synchrotron X-ray diffractometry revealed dehydration of LSP resulting in LSM, while anhydrous oxalic acid transformed to its dihydrate. In formulations of LSP, chemical degradation of levothyroxine sodium may be preceded by its partial dehydration.
Asunto(s)
Desecación , Composición de Medicamentos/métodos , Tiroxina/química , Estabilidad de Medicamentos , Almacenaje de Medicamentos , Excipientes/química , Humedad , Hipotiroidismo/tratamiento farmacológico , Comprimidos , Tiroxina/uso terapéutico , Difracción de Rayos XRESUMEN
Halogen lamp irradiation of benzo[e][1,2,4]triazines 2[X] in CH2Cl2 solutions leads to planar ring-fused 1,4-dihydro[1,2,4]triazin-4-yl radicals 1 through a novel, potentially general, cyclization mechanism. The scope and efficiency of the method were established for unsubstituted and ortho-substituted (X = NH2, Br, NO2) phenoxy, naphthyloxy, and quinolinoxy derivatives 2[X]. The regioselectivity of 2[X] photocyclization was rationalized with DFT computational methods. Radicals 1 were characterized by spectroscopic (UV-vis, EPR), electrochemical, and XRD methods.
RESUMEN
The inaugural crystallographic characterization of chromium(i)tricarbonyltris(phosphine) radicals has been achieved. Oxidation of [PPN][Cr(CO)3(PhBP)] (PhBP = [PhB(CH2PPh2)3]-) and analogous Cr(0) complexes featuring 3,5-dimethylphenyl and 3,5-bis(trifluoromethyl)phenyl borate substituents affords charge-neutral Cr(CO)3(PhBP) zwitterions, containing the first fully characterized [Cr(CO)3P3]+ units. The stabilization affected by the intramolecular charge separation established by PhBP ligands dramatically increases the robustness of these seventeen-electron Cr(i) complexes. Previous attempts to isolate salts of mer/fac-[Cr(CO)3P3]+ were frustrated by the thermal instability of these cations. The EPR spectroscopic data of Cr(CO)3(PhBP) supports Rieger's hypothesized low temperature preparation of fac-[Cr(CO)3{CH3C(CH2PPh2)3}]+. The robust [Cr(CO)3P3]+ unit of Cr(CO)3(PhBP) motivated the preparation of structurally characterized Cr(0)/Cr(i) (Cr(CO)3{η6-(PhBP)Cr(CO)3}, Cr(CO)3{η6-(((3,5-CH3)C6H3)BP)Cr(CO)3}) and W(0)/Cr(i) (W(CO)3{η6-(((3,5-CH3)C6H3)BP)Cr(CO)3}) complexes. While these bimetallics feature classical κ3-phosphine and η6-arene metal-binding, they are noteworthy since all other reported mixed-valent Cr(0)/Cr(i) complexes exhibit (a) significant thermal instability that has precluded their isolation and (b) greater uncertainty regarding the presence of distinct Cr(i) and Cr(0) centers. This work illustrates the utility of tris(phosphino)borates for the stabilization of cationic metal fragments within zwitterions that are inaccessible or difficult to characterize independently.
RESUMEN
This work describes the unexpected formation of an unusual phosphonium ylide when attempting the synthesis of bisphosphonium pyromellitic diimides. Spectroscopic and crystallographic characterization reveals that a combination of π-π and CHâ â â O interactions leads to supramolecular homodimerization of the ylide both in solution and in the solid-state. Only strong acids are able to protonate the ylide, which is otherwise inert to Wittig and alkylation reactivity. Taken together, these observations indicate that this compound is one of the most highly stabilized phosphonium ylides discovered to date.
RESUMEN
Understanding H2 binding and activation is important in the context of designing transition metal catalysts for many processes, including hydrogenation and the interconversion of H2 with protons and electrons. This work reports the first thermodynamic and kinetic H2 binding studies for an isostructural series of first-row metal complexes: NiML, where M = Al (1), Ga (2), and In (3), and L = [N(o-(NCH2PiPr2)C6H4)3]3-. Thermodynamic free energies (ΔG°) and free energies of activation (ΔG ) for binding equilibria were obtained via variable-temperature 31P NMR studies and lineshape analysis. The supporting metal exerts a large influence on the thermodynamic favorability of both H2 and N2 binding to Ni, with ΔG° values for H2 binding found to span nearly the entire range of previous reports. The non-classical H2 adduct, (η2-H2)NiInL (3-H2), was structurally characterized by single-crystal neutron diffraction-the first such study for a Ni(η2-H2) complex or any d10 M(η2-H2) complex. UV-Vis studies and TD-DFT calculations identified specific electronic structure perturbations of the supporting metal which poise NiML complexes for small-molecule binding. ETS-NOCV calculations indicate that H2 binding primarily occurs via H-H σ-donation to the Ni 4p z -based LUMO, which is proposed to become energetically accessible as the Ni(0)âM(iii) dative interaction increases for the larger M(iii) ions. Linear free-energy relationships are discussed, with the activation barrier for H2 binding (ΔG ) found to decrease proportionally for more thermodynamically favorable equilibria. The ΔG° values for H2 and N2 binding to NiML complexes were also found to be more exergonic for the larger M(iii) ions.
RESUMEN
Treatment of bis(mesitylene)niobium(0) with 6-7 equivalents of 2,6-dimethylphenyl isocyanide (CNXyl) affords two products with the empirical formula Nb(CNXyl)n (n = 7 or 6), which have been shown to be the diamagnetic dimers bis[µ-N,N',N'',N'''-tetrakis(2,6-dimethylphenyl)squaramidinato(2-)]bis[pentakis(2,6-dimethylphenyl isocyanide)niobium(I)], [Nb2(C9H9N)10(C36H36N4)] or [Nb(CNXyl)5]2[µ-C4(NXyl)4]·xSolvent, 1, and bis[µ-N,N',N'',N'''-tetrakis(2,6-dimethylphenyl)squaramidinato(2-)]bis[tetrakis(2,6-dimethylphenyl isocyanide)niobium(I)] tetrahydrofuran trisolvate, [Nb2(C9H9N)8(C36H36N4)]·3C4H8O or [Nb(CNXyl)4]2[µ-C4(NXyl)4]·3THF (THF = tetrahydrofuran), 2. Each contains NbI bound to either five or four terminal isocyanides, respectively, and to an unprecedented bridging tetraarylsquaramidinate(2-) unit, coordinated as a bidentate ligand to each niobium center, symmetrically due to the crystallographic inversion center that coincides with the centroid of the central C4 unit. Thus, in the presence of CNXyl, the bis(mesitylene)niobium(0) is oxidized to niobium(I), resulting in the facile loss of both mesitylene groups and the reductive coupling of two CNXyl groups per niobium to provide the first examples of tetraarylsquaramidinate(2-) ligands, [cyclo-C4N4Ar4]2-, coordinated to metals. In contrast, bis(mesitylene)niobium(0) reacts with the more crowded 2,6-diisopropylphenyl isocyanide (CNDipp) to afford the paramagnetic monomer hexakis(2,6-diisopropylphenyl isocyanide)niobium(0), [Nb(C13H17N)6] or Nb(CNDipp)6, 3, the first zero-valent niobium isocyanide analog of the highly unstable Nb(CO)6, which is presently only known to exist in an argon matrix at 4.2â K.
RESUMEN
Structures and spectroscopic characterization of the divalent complexes cis-dibromidotetrakis(2,6-dimethylphenyl isocyanide)iron(II) dichloromethane 0.771-solvate, [FeBr2(C9H9N)4]·0.771CH2Cl2 or cis-FeBr2(CNXyl)4·0.771CH2Cl2 (Xyl = 2,6-dimethylphenyl), trans-dibromidotetrakis(2,6-dimethylphenyl isocyanide)iron(II), [FeBr2(C9H9N)4] or trans-FeBr2(CNXyl)4, trans-dibromidotetrakis(2,6-dimethylphenyl isocyanide)cobalt(II), [CoBr2(C9H9N)4] or trans-CoBr2(CNXyl)4, and trans-dibromidobis(2,6-dimethylphenyl isocyanide)nickel(II), [NiBr2(C9H9N)2] or trans-NiBr2(CNXyl)2, are presented. Additionally, crystals grown from a cold diethyl ether solution of zero-valent Fe(CNXyl)5 produced a structure containing a cocrystallization of mononuclear Fe(CNXyl)5 and the previously unknown dinuclear [Fe(CNXyl)3]2(µ2-CNXyl)3, namely pentakis(2,6-dimethylphenyl isocyanide)iron(0) tris(µ2-2,6-dimethylphenyl isocyanide)bis[tris(2,6-dimethylphenyl isocyanide)iron(0)], [Fe(C9H9N)5][Fe2(C9H9N)9]. The (M)C-N-C(Xyl) angles of the isocyanide ligand are nearly linear for the metals in the +2 oxidation state, for which the ligands function essentially as pure donors. The νCN stretching frequencies for these divalent metal isocyanides are at or above that of the free ligand. Relative to FeII, in the structure containing iron in the formally zero-valent oxidation state, the Fe-C bond lengths have shortened, the C[triple-bond]N bond lengths have elongated, the (M)C-N-C(Xyl) angles of the terminal CNXyl ligands are more bent, and the νCN stretching frequencies have shifted to lower energies, all indicative of substantial M(dπ)âπ* backbonding.
RESUMEN
The crystal structures of chelates formed between each stable paramagnetic lanthanide ion and the octadentate polyamino carboxylate ligand DOTMA are described. A total of 23 individual chelates structures were obtained; in each chelate the coordination geometry around the metal ion is best described as a twisted square antiprism (torsion angle -25.0°--31.4°). Despite the uniformity of the general coordination geometry provided by the DOTMA ligand, there is a considerable variation in the hydration state of each chelate. The early Ln3+ chelates are associated with a single inner sphere water molecule; the Ln-OH2 interaction is remarkable for being very long. After a clear break at gadolinium, the number of chelates in the unit cell that have a water molecule interacting with the Ln3+ decreases linearly until at Tm3+ no water is found to interact with the metal ion. The Ln-OH2 distance observed in the chelates of the later Ln3+ ions are also extremely long and increase as the ions contract (2.550-2.732â Å). No clear break between hydrated and dehydrated chelates is observed; rather this series of chelates appear to represent a continuum of hydration states in which the ligand gradually closes around the metal ion as its ionic radius decreases (with decreased hydration) and the metal drops down into the coordination cage.
RESUMEN
Treatment of tetraethylammonium hexacarbonyltantalate, [Et4N][Ta(CO)6], with 1.1 equivalents of molecular iodine (I2) in tetrahydrofuran (THF) at 200â K, followed by the addition of 6.0 equivalents of 2,6-diisopropylphenyl isocyanide (CNDipp) and slow warming to 293â K over a 24â h period gave the tantalum(I) iodide derivative hexakis(2,6-diisopropylphenyl isocyanide-κC)iodidotantalum(I), [TaI(C13H17N)6] or TaI(CNDipp)6, 1. Recrystallization of this substance from pentane provided deep-red nearly black parallelepipeds of the product, which was characterized by single-crystal X-ray diffraction. Addition of 1 in THF at 200â K to a suspension of an excess (5.8 equivalents) of caesium graphite (CsC8), followed by warming, filtration, and solvent removal, afforded a dark-green oily solid of unknown composition, from which several red-brown rhombohedral plates of the ditantalum salt heptakis(2,6-diisopropylphenyl isocyanide-κC)tantalum hexakis(2,6-diisopropylphenyl isocyanide-κC)tantalate, [Ta(C13H17N)7][Ta(C13H17N)6] or [Ta(CNDipp)7][Ta(CNDipp)6], 2, were harvested. Salt 2 is a unique substance, as it is the only known example of a salt containing a homoleptic cation, [MLx]+, and a homoleptic anion, [MLy]-, with the same transition metal and π-acceptor ligand L. In solution, 2 undergoes full comproportionation to afford the recently reported 17-electron paramagnetic zerovalent tantalum complex Ta(CNDipp)6, the only known isolable TaL6 complex of Ta0.
RESUMEN
The synthesis, absolute stereochemical configuration, complete biological characterization, mechanism of action and resistance, and pharmacokinetic properties of ( S)-(-)-acidomycin are described. Acidomycin possesses promising antitubercular activity against a series of contemporary drug susceptible and drug-resistant M. tuberculosis strains (minimum inhibitory concentrations (MICs) = 0.096-6.2 µM) but is inactive against nontuberculosis mycobacteria and Gram-positive and Gram-negative pathogens (MICs > 1000 µM). Complementation studies with biotin biosynthetic pathway intermediates and subsequent biochemical studies confirmed acidomycin inhibits biotin synthesis with a Ki of approximately 1 µM through the competitive inhibition of biotin synthase (BioB) and also stimulates unproductive cleavage of S-adenosyl-l-methionine (SAM) to generate the toxic metabolite 5'-deoxyadenosine. Cell studies demonstrate acidomycin selectively accumulates in M. tuberculosis providing a mechanistic basis for the observed antibacterial activity. The development of spontaneous resistance by M. tuberculosis to acidomycin was difficult, and only low-level resistance to acidomycin was observed by overexpression of BioB. Collectively, the results provide a foundation to advance acidomycin and highlight BioB as a promising target.
Asunto(s)
Antituberculosos/farmacología , Proteínas Bacterianas/antagonistas & inhibidores , Mycobacterium tuberculosis/efectos de los fármacos , Mycobacterium tuberculosis/enzimología , Sulfurtransferasas/antagonistas & inhibidores , Tiazolidinas/farmacología , Tuberculosis/microbiología , Animales , Antituberculosos/síntesis química , Antituberculosos/química , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Productos Biológicos/síntesis química , Productos Biológicos/química , Productos Biológicos/farmacología , Biotina/biosíntesis , Caproatos/síntesis química , Caproatos/química , Caproatos/farmacología , Farmacorresistencia Bacteriana , Humanos , Cinética , Ratones , Pruebas de Sensibilidad Microbiana , Mycobacterium tuberculosis/química , Mycobacterium tuberculosis/genética , Sulfurtransferasas/química , Sulfurtransferasas/genética , Sulfurtransferasas/metabolismo , Tiazolidinas/síntesis química , Tiazolidinas/química , Tuberculosis/tratamiento farmacológicoRESUMEN
Two synthetic methods were developed for the synthesis of PDE3A inhibitor ORG9935. The first one proceeds in six steps and 34% overall yield and the second one in five steps and an overall yield of 69% starting from commercially available starting material 5,6-dimethoxybenzo[b]thiophene-2-carboxylic acid (6). The enantiomers of ORG9935 were separated by chiral column chromatography and the absolute stereochemistry of the (+)-enantiomer, ORG20865 was determined by X-ray crystallography to possess the (S)-configuration. The (-)-enantiomer, ORG20864, was therefore assigned the (R)-stereochemistry. The biologically less active (+)-isomer ORG20865 was converted to racemic ORG9935 under basic conditions, which then can be separated again into the enantiomers. The crystal structure of ORG20865 is notable for having the highest Z' for any known pharmaceutical substance.