Occurrence of Microplastics in Most Consumed Fruits and Vegetables from Turkey and Public Risk Assessment for Consumers.

Microplastics are transferred to humans through the food chain by consuming food contaminated with microplastics. However, the knowledge about the risks of dietary exposure for humans to these particles is very limited. Moreover, only a few studies on microplastic pollution in fruit and vegetables have been carried on. Thus, this study aims to investigate the presence of microplastics in some of the most consumed fruits and vegetables (pear (Pyrus communis), apple (Malus domestica), tomato (Solanum lycopersicum), onion (Allium cepa), potatoes (Solanum tuberosum), and cucumber (Cucumis sativus)) from Turkey and to evaluate the potential risk for consumers. Fruits and vegetable samples were purchased from different markets and fruiterer (two of each) in Mugla province, Southwest of Turkey. Microplastic extraction processes were carried out on the edible parts of the samples. According to the results obtained, a total of 210 particles (2.9 ± 1.6 particle g-1) were detected in all samples. Any significant difference occurred among the different markets. The maximum average amount of microplastic was determined in tomato samples (3.63 ± 1.39 particle g-1). The highest microplastic intake was with tomato (398,520 particles individual-1 year-1 for Estimated Annual Intake (EAI) and Estimated Daily Intake (EDI) for children 68.24 particles kg-1 day-1). The occurrence of microplastics of big size, that are not allowed to pass by plant xylem transport, suggests that fresh vegetables and fruits can be contaminated with plastic, especially during the production phase, during agricultural activities and during the marketing process (transport to the market and purchasing process).

Effects of inorganic arsenic species on the antioxidant enzyme system of the Amazon Sword Plant (Echinodorus amazonicus Rataj).

This study aims to examine the effects of Arsenite (As+3) and Arsenate (As+5) on the aquatic macrophyte Amazon Sword Plant (Echinodorus amazonicus Rataj). To this aim, different concentrations of As+3 and As+5 (0, 6, 18 and 54 µM) were analyzed. At the end of the trail, photosynthetic pigment contents, total protein amounts, the enzymatic antioxidants superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) activities and the amount of malondialdehyde (MDA) in the leaf samples of E. amazonicus were investigated. The antioxidant enzyme activities increased at low concentrations (32.13% for SOD, 185% for CAT and 201.5% for POX in the groups of 6 µM As+5), but decreased at high concentrations (64.98% for SOD, 21.64% for CAT and 21.29% for POX in the groups of 54 µM As+3). MDA increased in all the treatment groups. The highest MDA contents were observed as 96% for 54 µM As+3 and 71.50% for 54 µM As+5. Photosynthetic pigment contents and the amount of protein were decreased with higher concentrations. The most significant decreases in protein content were 65% for 54 µM As+3 and 34.9% for 54 µM As+5. As a result, the toxicity of As+3 was higher and the toxic effect increased at higher concentrations.

Investigation of microplastics in edible wild mussels from Izmir Bay (Aegean Sea, Western Turkey): A risk assessment for the consumers.

The presence of microplastics (MPs) was determined in Mediterranean mussel (Mytilus galloprovincialis Lamarck, 1819) and grooved carpet shell (Ruditapes decussatus Linnaeus, 1758) together with water samples from in Izmir Bay (Aegean Sea, Western Turkey). A total of 895 MPs from Karsiyaka (Station 1), and 787 MPs from Gülbahçe (Station 2) were detected in 60 mussel samples, giving a total content of 1682 MPs. In water samples, a total of 545 MPs were detected in samples from Station 1 and 1287 MPs in samples from Station 2 (1832 MPs total). The amount of MPs in water samples was less in Station 1 than in Station 2. This difference is probably caused by Çigli Wastewater Treatment Plant (WWTP) located near Station 1. Public health risk assessment on the consumption of the species M. galloprovincialis revealed that 70.82 items person-1 year-1 MPs through this species could enter consumers' digestive systems.

Heavy metals in processed seafood products from Turkey: risk assessment for the consumers.

In this study, concentrations of 6 different elements (copper (Cu), tin (Sn), zinc (Zn), iron (Fe), cadmium (Cd) and lead (Pb)) in 9 different processed seafood (marinated and smoked mackerel, smoked mackerel, marinated octopus tentacles, marinated octopus salad, marinated squid salad, salted and dried mackerel, marinated and smoked anchovy, smoked salmon and smoked bonito) randomly taken from markets in Mugla province (West Anatolia, Turkey) were determined with the method of inductively coupled plasma mass spectroscopy (ICP-MS) after microwave wet digestion process. In addition, risk assessment for consumer health was conducted by the determination of estimated daily intake (EDI), target hazard quotient (THQ), total target hazard quotient (TTHQ) and carcinogenic risk (CR). According to the obtained results, the seafood product samples with the highest concentrations of each metal were marinated and smoked anchovy for Fe (65.85 ± 16.03 mg kg-1), marinated and smoked anchovy for Zn (64.58 ± 25.16 mg kg-1), marinated octopus salad for Cu (26.33 ± 17.76 mg kg-1), smoked mackerel for Sn (0.42 ± 0.18 mg kg-1), smoked mackerel for Pb (0.28 ± 0.18 mg kg-1) and marinated squid salad for Cd (0.31 ± 0.12 mg kg-1). According to risk assessment for consumer health, it was found that EDI results did not exceed the tolerable daily intake (TDI) values. As a conclusion, any risk for consumer health in terms of CR, THQ and TTHQ values was not detected in the examined samples.

Microplastic pollution at the intersection of the Aegean and Mediterranean Seas: A study of the Datça Peninsula (Turkey).

This study aims to reveal microplastic pollution. It is the first to be conducted in the South Aegean Region and one of the limited numbers of such studies in Turkey. To this aim, in September 2018 microplastic particles (MP's) pollution was evaluated on the sandy beaches along the coastline of the Datça Peninsula (Southern Aegean coast of Turkey) located at the intersection of the Aegean and Mediterranean Seas. MP's were analyzed using standard operating procedure along with some experimental techniques. Prior to the identification process, microplastics ranging from 1 to 5 mm were exposed to an experimental pre-treatment and efficient polymer scanning. The results showed that fragments (72%) were the dominant category from all the beaches exposed to dominant winds and surface currents and that MP contamination in the Datça Peninsula was higher than that reported in the literature from similar locations around the world (1154.4 ±â€¯700.3 particles kg-1 dw). More specifically the highest contamination was found in Aktur Beach (2073.3 ±â€¯648.6 particles kg-1 dw) which could be considered as a hotspot for the whole region in terms of MP pollution. As a result of the study, as sampling stations are remote from major cities and industrial zones, maritime activities increase during summer season and tar has been observed on particles during analyses, it can be asserted that the pollution may be caused by marine resources.

Heavy metal contamination in the muscle of Aegean chub (Squalius fellowesii) and potential risk assessment.

Especially after the industrial revolution, the amount of contaminants released in aquatic ecosystems has considerably increased. For this reason, the necessity to carry on research on the existence of contaminants, specifically heavy metals, has emerged. In this study, heavy metal concentrations in muscle tissues of Aegean chub, which was an endemic species of south western part of Turkey, gathered from Tersakan River were examined. Heavy metal concentrations of the samples were analyzed with ICP-MS. Estimated daily intakes (EDI), target hazard quotient (THQ), and carcinogenic risk (CR) of elements were calculated. The heavy metals detected in muscle tissues were Zn > Cu > Cr > Mn > Pb > Cd, consecutively. According to the results of the applied health risk assessment (EDI, THQ and CR) for heavy metal exposure from fish consumption in children and adults, it was determined that there was no any significant threat to human health.

Heavy metals in tissues of scorpionfish (Scorpaena porcus) caught from Black Sea (Turkey) and potential risks to human health.

Scorpionfish (Scorpaena porcus) is a demersal fish species commercially important for its of which meat is tough and delicious. The aim of this study was to determine heavy metal (Al, Cu, Ni, As, Cd, Hg, Pb, U) concentrations in this fish species which is traditionally consumed in the Black Sea Area and, to compare the concentrations of various toxic elements in different organs of the fish specimens (muscle, liver, gill, and skin). Within this scope, the mineralization was performed using microwave digestion system. Thirty-two scorpionfish caught from Sinop Inland Port during 2010 were analyzed. The heavy metal concentrations were determined with the method of inductively coupled plasma mass spectroscopy (ICP-MS). Verification of the method was demonstrated by analysis of standard reference material (NRCC-TORT-2 lobster hepatopancreas). After evaluation of the results, it was determined that the highest heavy metal accumulation was generally found in the liver. The maximum aluminum level and the minimum uranium level were found in the analyzed tissues. In terms of heavy metals, Al, Cu, Cd, and Hg showed a statistically significant difference between tissues (p < 0.05). It was determined that heavy metal concentrations obtained from the muscle tissues did not exceed the national and international recommended limits; and also it was found that daily intake amounts did not exceed tolerable daily intake amounts. Furthermore, in THQ based risk evaluation, the value 1 which is crucial for children and adults was not surpassed. In terms of public health, it was found out that there was not any risk in consumption of scorpionfish in the study area.

Heavy metal accumulation in the leaves, stem and root of the invasive submerged macrophyte Myriophyllum spicatum L. (Haloragaceae): an example of Kadin Creek (Mugla, Turkey)

In this study, the existence of heavy metals such as chromium (Cr), arsenic (As), cadmium (Cd), mercury (Hg) and lead (Pb) their distribution in the organs of plant and their bioaccumulation rates in water samples and Myriophyllum spicatum which were collected from the Kadin Creek in seasonal periods between 2011 and 2012 were analyzed. Heavy metal concentrations in the mineralized plant and water samples were determined with Inductive coupled plasma with mass spectroscopy (ICP-MS). Results showed the maximum heavy metal concentrations, in the root and minimum heavy metal concentrations, except chromium, in the stem. The distribution of heavy metals As, Cd, Hg and Pb was in the form of root > leaf > stem; the distribution of Cr was in the root > stem > leaf. There was a strong negative correlation between the suspended solid matter and heavy metal concentrations in the plant tissues. Heavy metal accumulation showed increase generally in fall. Heavy metals in the water were sorted as Pb > Cr > As > Hg > Cd by their mean concentrations. According to the factor data of bioconcentration, the order of heavy metal accumulation in the plant was As > Cr > Pb > Hg > Cd.