RESUMEN
The role of low-grade inflammation in the development of postinfectious irritable bowel syndrome (PIIBS) has attracted increasing attention. Abnormal CD11c+ mononuclear phagocytes, such as dendritic cells (DCs), macrophages, and monocytes, are involved in the disruption of immune tolerance in organisms, which can lead to the development of chronic inflammatory diseases. The present study tested the hypothesis that CD11c+ lamina propria mononuclear phagocytes (CD11c+ LPMPs) contribute to increased mucosal permeability and visceral hypersensitivity in a PIIBS mouse model. CD11c+ LPMPs were isolated and purified via the digestion of intestinal tissues and magneticactivated cell sorting. We detected increased mucosal permeability, visceral hypersensitivity and intestinal inflammation during both the acute and chronic stages of Trichinella infection. Following the transfer of CD11c+ LPMPs from PIIBS mice into normal mice, lowgrade inflammation was detected, as demonstrated by increased IL4 expression in the ileum, as well as enhanced mucosal permeability, as indicated by decreased transepithelial electrical resistance and the pre-sence of ultrastructural alterations. More importantly, the mice that underwent adoptive transfer of CD11c+ LPMPs from the PIIBS mice also exhibited increased abdominal withdrawal reflex scores and a decreased threshold. Our data demonstrated that the CD11c+ LPMPs from this PIIBS mouse model were not only able to transfer enteric inflammation to the normal mice but also caused abnormal intestinal function, characterized by epithelial barrier disruption and visceral hyperalgesia.
Asunto(s)
Antígeno CD11c/inmunología , Hiperalgesia/patología , Mucosa Intestinal/patología , Síndrome del Colon Irritable/patología , Sistema Mononuclear Fagocítico/patología , Animales , Células Cultivadas , Hiperalgesia/inmunología , Hiperalgesia/parasitología , Inflamación/inmunología , Inflamación/parasitología , Inflamación/patología , Absorción Intestinal , Mucosa Intestinal/inmunología , Mucosa Intestinal/parasitología , Síndrome del Colon Irritable/inmunología , Síndrome del Colon Irritable/parasitología , Masculino , Ratones , Sistema Mononuclear Fagocítico/inmunología , Sistema Mononuclear Fagocítico/parasitología , Membrana Mucosa/citología , Membrana Mucosa/inmunología , Membrana Mucosa/parasitología , Membrana Mucosa/patología , Trichinella spiralis/inmunología , Triquinelosis/inmunología , Triquinelosis/parasitología , Triquinelosis/patología , Vísceras/inmunología , Vísceras/parasitología , Vísceras/patologíaRESUMEN
This study aimed to investigate the expression of ß-catenin in hepatocellular carcinoma (HCC) tissues and its relationship with α-fetoprotein (AFP) in HCC. Immunohistochemistry was used to determine the expression of ß-catenin in normal liver tissues (n=10), liver cirrhosis tissues (n=20), and primary HCC tissues (n=60). The relationship between ß-catenin expression and clinical parameters of HCC was investigated. Real-time PCR and Western blotting were used to detect the mRNA and protein expression levels of ß-catenin in the liver cancer cell line SMMC-7721 transfected with a plasmid encoding AFP, and also the mRNA and protein expression levels of ß-catenin were measured in the liver cancer cell line Huh7 before and after the transfection with AFP shRNA plasmids. The results showed that ß-catenin was only expressed on the cell membrane in normal liver tissues. Its localization to the cytoplasm and nucleus of cells was observed in a small proportion of cirrhotic tissues or adjacent HCC tissues, and such ectopic expression of ß-catenin was predominant in HCC tissues. The abnormal expression of ß-catenin was correlated with serum AFP levels, cancer cell differentiation and vascular invasion (P<0.05). Additionally, the increased expression of AFP resulted in the upregulation of ß-catenin mRNA and protein levels, while knockdown of AFP with AFP shRNA led to significantly decreased ß-catenin mRNA and protein levels (P<0.05). It was suggested that the abnormal expression of ß-catenin is implicated in hepatic carcinogenesis and development. AFP can lead to increased expression of ß-catenin, which may account for the poor prognosis of AFP-associated HCC patients.