[The correlation between functional magnetic resonance imaging features and expression of Her-2 in breast cancer].

Objective: To explore the correlation between dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), magnetic resonance diffusion-weighted imaging (DWI) images of breast cancer and human epidermal growth factor receptor-2 (Her-2) expression. Methods: A retrospective analysis of 84 patients with breast cancer confirmed by biopsy from January 2012 to December 2016 in Zhejiang University Lishui Hospital was conducted. The data of DCE-MRI, DWI scanning were collected before surgery and Her-2 was detected by immunohistochemistry (IHC). Then, all the patients were divided into Her-2 positive group and Her-2 negative group according to Her-2 expression. There were 44 cases in the Her-2 positive group and 40 cases in the Her-2 negative group. The differences in the characteristics of the lesion morphology, time-signal intensity curve (TIC), early enhancement rate and apparent diffusion coefficient (ADC) were compared, and their correlation with Her-2 expression was analyzed. Results: Of the 84 lesions, 12 were orthotopic ductal carcinoma, 6 were lobular carcinoma, and 66 were invasive ductal carcinoma. There were significant differences in lesion morphology (P=0.012) and TIC curve morphology (P=0.038) between Her-2 positive group and negative group. At the same time, the early enhancement rate (P=0.012) and ADC value (P=0.038) of Her-2 positive group were significantly higher than those of negative group. Except morphology of segmental lesions and TIC curve morphology, other characteristic parameters were correlated to Her-2 (all P<0.05). The correlation coefficients between early enhancement rate, ADC value and Her-2 expression were as high as 0.758 and 0.809 (all P<0.05). Conclusions: The morphology of breast cancer lesions, time-signal intensity curve, early enhancement rate and ADC value are all correlated with Her-2 expression.The early enhancement rate and ADC value are significantly positively correlated with Her-2 expression.

The ability of the Coincidence Doppler Broadening Spectroscopy to characterize polymers containing different chemical elements.

Hydrocarbon polymers, O-containing, F-containing and Cl-containing polymers are comprehensively studied by Coincidence Doppler Broadening Spectroscopy (CDBS). It is shown that for polymers with different chemical structure, CDBS results can effectively distinguish polar groups CO, CCl, and CF. For polymers with similar chemical structure, the intensity of the element-specific peak in the CDBS ratio curve is dependent not only on the fraction of free positrons, but also on the content of characteristic atom in polymer repeated unit, and the polarity of the polymer molecule. For polymers containing several different polar groups, such as PCTFE (CF & CCl) and PFA (CF & CO), whether the element-specific peak appears or not depends on the amount of the polar groups and its positron capture ability. This work may provide insights into potential applications of CDBS for studying complex polymer systems.

Probing head-to-toe deformation law assessment for abdominal tumor through respiratory movement simulation and CTVision radiation research.

This paper aims to explore head-to-toe deformation law for abdominal tumor with CTVision and selfdesigned respiratory movement simulation mold and meanwhile verify the accuracy and correctness of the treatment. In experimental group, a self-designed respiratory movement mold was used. The image was scanned out by CT scanning based on the movement state and then sent to the planning system to compare the location variation of tumor and formulating the treatment plan accordingly, followed by verification and verified derivation values observation. A total of 21 cases of abdominal tumor were included in the case group. Their breathing movement was detected under a simulated locator and then the data was recorded. The image was scanned and sent to the planning system to compare the location variation of the tumor, the patients then underwent 3D conformal therapy (3D-CRT) and we performed verification and observed verified derivation values. Finally, the results of the case group and the experimental group were compared. The mean of the verified derivation values was smaller than respiratory motion values in experimental group (t=-10.78, P=0, P < 0.05); the mean of verified derivation values of the patients was smaller than respiratory motion values in group f, g, h, i, j, l, n, o, p, q, r, s t, u in the case group (P < 0.05); no remarkable difference was found between the two values in group a, b, c, k and m (P < 0.05); group e was unable to undergo the statistical test since its standard deviation was 0; the mean of the verified derivation values was higher than respiratory motion values in group d (P < 0.05). In conclusion, radiation therapy applied with CTVision proved to be accurate and convincing in the treatment of abdominal tumor.

Pancreatic schwannoma: CT findings.

Schwannomas of the pancreas are rare tumors. We report a case of solitary schwannoma of the pancreas without von Recklinghausen disease. A 72-year-old man underwent routine abdominal ultrasound before cholecystectomy because of chronic cholecystitis and multiple gallstones, and ultrasound showed a small solitary tumor in the pancreas. Spiral computed tomography showed a 1.0-cm, well-defined homogeneous hypodense lesion with mild inhomogeneous enhancement and peripheral enhancement at the junction between the head and body of the pancreas. These computed tomographic findings are different from those in previous reports.

[Isolation, purification and renaturation of recombinant-DNA-derived porcine somatotropin].

Large scale abstraction and isolation of bacterially synthesized, recombinant-DNA-derived, porcine growth hormone (r-pST) is described. The r-pGH is found in genetic engineering E. coli as the form of inclusion bodies. Pellet fraction which were mainly inclusion bodies, after cell breakage and centrifugation, were collected. Cell envelope components, such as protein, lipid, endotoxin and nucleic acids are selectively removed from the pellet fraction by an EDTA/lysozyme/deoxycholate extraction. Inclusion bodies were dissolved using 6 mol/L guanidine/HCl and air oxidation is then carried out in the presence of the guanidine/HCl. The Guanidine/HCl protein mixture were diluted by renaturation solution. Guanidine/HCl were removed by dialysis and then correctly refolded, oxidized r-pGH were obtained. Injection experiment of hypophysectomized rats proved r-pST with high native bioactivity was obtained.

Improved Procedure for the Estimation of Nanogram Quantities of Indole-3-acetic Acid in Plant Extracts using the Indolo-alpha-pyrone Fluorescence Method.

The indolo-alpha-pyrone fluorescence method of determining indole-3-acetic acid (IAA) is improved by adding butylated hydroxytoluene (BHT), an antioxidant, to samples: addition of BHT increases the fluorescence intensities and decreases their variability so that amounts of IAA as small as 0.1 to 1 nanogram become measurable. Interfering compounds, 4-chloroindole-3-acetic acid and 5-hydroxyindole-3-acetic acid, can be separated from IAA by thin-layer chromatography using polyamide as the solid support, and benzene-ethyl acetate-acetic acid (70:25:5, v/v) as the developing solvent. Polyamide thin-layer chromatography is also superior in purifying IAA without significant loss or decomposition.A serious problem in extracting IAA from plant tissues is the considerable loss. Losses can result from decomposition of IAA when its solutions in organic solvents are evaporated to dryness, apparently due to some impurity in the solvents. Decomposition can be eliminated by simple treatments of the solvents, i.e. addition of BHT, washing with water, and passage through cotton wool. The latter is particularly effective in the case of ethyl acetate and ether. Loss of IAA by photodecomposition is also substantial even in dried samples. Ways to minimize the conversion of indole-3-pyruvic acid to IAA during extraction procedure are also described.A revised procedure of IAA extraction and determination is proposed. Applied to etiolated shoots of maize seedlings, it enables content to be estimated (18 nanograms per gram fresh weight) with over 90% recovery in terms of [(14)C]IAA added at the beginning of the extraction.