KYNU Expression Promotes Cisplatin Resistance in Esophageal Cancer.

Background: Chemotherapy resistance is a barrier to effective cancer prognoses. Cisplatin (CDDP) resistance is a major challenge for esophageal cancer (EC) therapy. A deeper understanding of the fundamental mechanisms of cisplatin resistance and improved targeting strategies are required in clinical settings. This study was performed to identify and characterize a marker of cisplatin resistance in EC cells. Method: KYSE140 and Eca-109 cells were subjected to escalating concentrations of cisplatin, resulting in the development of cisplatin-resistant KYSE140/CDDP and Eca-109/CDDP cell lines, respectively. RNA Sequencing (RNA-seq) was utilized to screen for the genes exhibiting differential expression between cisplatin-resistant and parental cells. Reverse transcription quantitative PCR was conducted to assess gene expression, and western blotting was employed to analyze protein levels. A sphere-formation assay was performed to validate tumor cell stemness. Cell counting kit-8 (CCK-8) experiments were conducted to confirm the sensitivity of cells to cisplatin. We examined the relationship between target genes and the clinicopathological features of patients with EC. Furthermore, the expression of target genes in EC tissues was evaluated via western blotting and fluorescence probe in situ hybridization (FISH). Results: KYNU was upregulated in cisplatin-resistant EC cells (KYSE140/CDDP and Eca-109/CDDP cells) and in EC tissues compared to that in the respective parental cell lines (KYSE140 and Eca-109 cells) and non-carcinoma tissues. Downregulation of KYNU increased cell sensitivity to cisplatin and suppressed tumor stemness, whereas abnormal KYNU expression had the opposite effect. KYNU expression was correlated with the expression of tumor stemness-associated factors (SOX2, Nanog, and OCT4) and the tumor size. Conclusions: KYNU may promote drug resistance in EC by regulating cancer stemness, and could serve as a biomarker and therapeutic target for EC.

Bidirectional Longitudinal Associations between Parental Psychological Control and Peer Victimization among Chinese Adolescents: The Mediating Role of Basic Psychological Need Satisfaction.

Despite evidence indicating a connection between inappropriate parenting styles and peer victimization, the dynamic processes and mechanisms underlying this link and whether it is consistent across genders and different developmental stages have yet to be explored. To address these gaps, the current 2-year longitudinal study explored the potential bidirectional associations between parental psychological control and peer victimization, as well as the mediating role of adolescent basic psychological need satisfaction. A total of 4,990 adolescents (49.4% boys, Mage T1 = 12.21 years, SDage T1 = 2.60) across different developmental stages (early adolescents, N = 1,819, 49.2% boys, Mage T1 = 9.34 years, SDage T1 = 0.62; middle adolescents, N = 1,525, 50.75% boys, Mage T1 = 12.47 years, SDage T1 = 0.69; late adolescents, N = 1,646, 46.5% boys, Mage T1 = 15.26 years, SDage T1 = 0.50) participated in this three-wave longitudinal survey. The results revealed that parental psychological control was bidirectionally associated with peer victimization. Additionally, basic psychological need satisfaction played the meditating role in this vicious cycle. Further analysis demonstrated interesting developmental differences. Parental psychological control was directly associated with subsequent peer victimization at all three developmental stages, and peer victimization was only directly associated with subsequent parental psychological control in the next year among early adolescents and middle adolescents. The mutual mediating role of basic psychological need satisfaction between parental psychological control and peer victimization was observed exclusively in early adolescents. Both male and female adolescents could be equally affected by these dynamics. This research underscores the reciprocal dynamics inherent in parent-child interactions, intervening in either of these processes (i.e., family, peers, and adolescent basic psychological need satisfaction) may break this destructive cycle.

Development of beliefs in a just world among Chinese early adolescents and the predictive role of family factors: A three-wave longitudinal study.

OBJECTIVE: This study explored how belief in a just world (BJW) develops among Chinese adolescents and the predictive role of family factors. BACKGROUND: The development of BJW in adolescence is an important but understudied topic, especially in non-Western contexts. METHOD: Using a three-wave longitudinal design, 1525 participants (48% girls; Mage = 12.47) were recruited to report their BJW, childhood SES, only-child or not, and parental psychological control in Wave 1 (Wave 2: N = 1262; Wave 3: N = 1124). RESULTS: The mean slope for personal BJW is positive and significant, but not significant for general BJW. Childhood SES predicted initial level of personal and general BJW and the rate of growth of personal BJW. Only-child predicted initial level and the growth rate of personal BJW. Parental psychological control negatively predicted personal and general BJW at three time points. CONCLUSION: Personal BJW increased during the observation period, whereas general BJW was stable. Individuals with lower levels of childhood SES had lower initial personal and general BJW but a higher growth rate in personal BJW than those with higher SES. Individuals having siblings had lower levels of initial personal BJW but a higher growth rate in personal BJW than those from only-child family. Parental psychological control may exert consistent and contemporaneous negative effect on BJW across time.

Research on Three-Dimensional Porous Composite Nano-Assembled α-MnO2/Reduced Graphene Oxides and Their Super-Capacitive Performance.

A series of three-dimensional porous composite α-MnO2/reduced graphene oxides (α-MnO2/RGO) were prepared by nano-assembly in a hydrothermal environment at pH 9.0-13.0 using graphene oxide as the precursor, KMnO4 and MnCl2 as the manganese sources and F- as the control agent of the α-MnO2 crystal form. The α-MnO2/RGO composites prepared at different hydrothermal pH levels presented porous network structures but there were significant differences in these structures. The special pore structure promoted the migration of ions in the electrolyte in the electrode material, and the larger specific surface area promoted the contact between the electrode material and the electrolyte ions. The introduction of graphene solved the problem of poor conductivity of MnO2, facilitated the rapid transfer of electrons, and significantly improved the electrochemical performance of materials. When the pH was 12.0, the specific surface area of the 3D porous composite material αMGs-12.0 was 264 m2·g-1, and it displayed the best super-capacitive performance; in Na2SO4 solution with 1.0 mol·L-1 electrolyte, the specific capacitance was 504 F·g-1 when the current density was 0.5 A·g-1 and the specific capacitance retention rate after 5000 cycles was 88.27%, showing that the composite had excellent electrochemical performance.

Cross-cultural variation in cooperation: A meta-analysis.

Impersonal cooperation among strangers enables societies to create valuable public goods, such as infrastructure, public services, and democracy. Several factors have been proposed to explain variation in impersonal cooperation across societies, referring to institutions (e.g., rule of law), religion (e.g., belief in God as a third-party punisher), cultural beliefs (e.g., trust) and values (e.g., collectivism), and ecology (e.g., relational mobility). We tested 17 preregistered hypotheses in a meta-analysis of 1,506 studies of impersonal cooperation in social dilemmas (e.g., the Public Goods Game) conducted across 70 societies (k = 2,271), where people make costly decisions to cooperate among strangers. After controlling for 10 study characteristics that can affect the outcome of studies, we found very little cross-societal variation in impersonal cooperation. Categorizing societies into cultural groups explained no variance in cooperation. Similarly, cultural, ancestral, and linguistic distance between societies explained little variance in cooperation. None of the cross-societal factors hypothesized to relate to impersonal cooperation explained variance in cooperation across societies. We replicated these conclusions when meta-analyzing 514 studies across 41 states and nine regions in the United States (k = 783). Thus, we observed that impersonal cooperation occurred in all societies-and to a similar degree across societies-suggesting that prior research may have overemphasized the magnitude of differences between modern societies in impersonal cooperation. We discuss the discrepancy between theory, past empirical research and the meta-analysis, address a limitation of experimental research on cooperation to study culture, and raise possible directions for future research. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

The Effect of Target's Power on Prosocial Behavior: A Cross-Cultural Study.

Social power predicts numerous important life outcomes and social orientations. Thus far, the research literature has mainly examined how an individual's own power shapes interactions with others, whereas whether a target's power affects social interactions has been relatively neglected. In particular, does a target's power have an effect on the agent's prosocial behavior? Furthermore, could culture along with the power distance dimension alter the effect of a target's power on prosocial behavior? To explore these two research questions, we investigated the effect of a target's power (power unspecified targets vs. powerful targets) on prosocial behavior in both China and the United States. Questionnaires measuring prosocial behavior toward power unspecified or powerful targets were distributed to Chinese and American emerging adults (n in total = 893). According to the results, both Chinese and Americans were less likely to help powerful targets compared with power unspecified targets. Moreover, the Chinese were less prosocial toward both power unspecified and powerful targets in comparison to the Americans. These findings highlight the key roles of a target's power and culture in shaping an individual's prosocial behavior.

Do male and female soccer players differ in helping? A study on prosocial behavior among young players.

Acting prosocially can be quite challenging in one of the most salient intergroup contexts in contemporary society: Soccer. When winning is the ultimate goal, balancing self-interest with helping a fellow player in distress can be a tough decision; yet it happens. To date, we know little about what motivates soccer players to offer such help in the heat of the game. We propose that sex and what is at stake will matter in such prosocial dilemma situations. A pilot study (N = 107) indicated that female players may be more likely to help than male players, but this difference was only observed when the players are close to scoring position rather than far away from the goal (midfield). The main study (N = 366) finds that young soccer players show elevated inclinations to help in low-stakes situations, for example when their team is winning or when the outcome of the game seems pretty much decided. Contrariwise, helping intentions decline in high-stakes situations, for example when one's own team is losing, when one is close to a scoring position in the offense (rather than at the midfield), or when the outcome of the game is still uncertain. Furthermore, female players show somewhat greater inclinations to help than their male counterparts. The current data point at some differences for male and female soccer players, albeit small in effect size. In contrast, we conclude that especially quick cost-benefit judgments regarding the stakes can play a major role in decisions to help or not to help another player on the soccer field.

Preparation of Graphite Oxide Containing Different Oxygen-Containing Functional Groups and the Study of Ammonia Gas Sensitivity.

A series of graphite oxide samples were prepared using the modified Hummers method. Flake graphite was used as the raw material and the reaction temperature of the aqueous solution was changed (0 °C, 30 °C, 50 °C, 60 °C, 70 °C, 80 °C, and 100 °C). X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectral analysis, X-ray photoelectron spectroscopy, and contact angle tests were performed to characterize the structure, chemical bonding, type, and content of oxygen-containing functional groups of the graphite oxide samples. The results showed that the type and content of each oxygen-containing functional group could be controlled by changing the reaction temperature with the addition of water. As the temperature of the system increased, the degree of oxidation of the graphite oxide samples first increased and then decreased. Too high a temperature (100 °C) of the system led to the formation of epoxy groups by the decomposition of some hydroxyl groups in the samples, causing the reduction of oxygen-containing functional groups between the graphite layers, poor hydrophilic properties, and low moisture content. When the system temperature was 50 °C, the interlayer spacing of the graphite oxide samples was at its highest, the graphite was completely oxidized (C/O = 1.85), and the oxygen-containing functional groups were mainly composed of hydroxyl groups (accounting for approximately 28.88% of the total oxygen-containing functional groups). The high content of hydroxyl and carboxyl groups had good hydrophilic ability and showed the highest moisture content. The sample at 50 °C had better sensitivity to ammonia because of its high hydroxyl group and carboxyl group content, with the sample showing an excellent profile when the ammonia concentration was 20⁻60 ppm.

Novel caged luciferin derivatives can prolong bioluminescence imaging in vitro and in vivo.

Based on N-cyclobutylaminoluciferin (cybLuc), a set of high and efficient caged bioluminescent derivatives (Clucs) as firefly luciferase pro-substrates has been developed herein. After careful examination, these molecules exhibited low cytotoxicity and prolonged bioluminescence imaging up to 6 h in vitro and in vivo. Importantly, these caged luciferin derivatives have the potential to serve as long-term tracking tools to explore some biological process by using bioluminescent imaging.

Prolonged bioluminescence imaging in living cells and mice using novel pro-substrates for Renilla luciferase.

The prodrug or caged-luciferin strategy affords an excellent platform for persistent bioluminescence imaging. In the current work, we designed and synthesized ten novel pro-substrates for Renilla luciferase by introducing ester protecting groups of different sizes into the carbonyl group of the free luciferin 1. Taking advantage of intracellular esterases, lipases, and nucleophilic substances, the ester protecting groups were hydrolyzed, resulting in the release of a free luciferin and a bioluminescence signal turn-on. Among the tested pro-substrates, the butyryloxymethyl luciferin 7 exhibited low cytotoxicity and a prolonged luminescence signal both in cellulo and in vivo. Therefore, the butyryloxymethyl luciferin 7 can act as a promising substrate for noninvasive extended imaging in diagnostic and therapeutic fields.

Effect of p53ß on human gastric cancer cells treated with recombinant mutated human TNF and cisplatin.

The present study aimed to investigate the role of tumour protein 53 isoform b (p53ß) on human gastric cancer (GC) cell lines treated with recombinant mutated human tumour necrosis factor (rmhTNF) and cisplatin. The Cell Counting Kit­8 assay was used to assess growth in the GC cell lines MKN45 and SGC7901, following treatment with rmhTNF in the presence or absence of cisplatin. Levels of p53ß and bcl­2 apoptosis regulator (bcl­2) mRNA were assessed using reverse transcription­polymerase chain reaction. The results demonstrated that growth was significantly inhibited by either cisplatin or rmhTNF treatments alone in MKN45 cells, and combination treatment with cisplatin and rmhTNF had a synergistic effect on growth inhibition of MKN45 cells. Notably, these observations were not evident in SGC7901 cells, where a mutant form of p53 is present. Treatment of MKN45 cells with rmhTNF did not affect bcl­2 or p53ß mRNA expression levels. However, treatment of MKN45 cells with cisplatin induced upregulation of p53ß and downregulation of bcl-2 mRNA expression levels, and these effects were enhanced by combination treatment with rmhTNF. Pearson correlation analysis revealed a negative correlation between the expression of p53ß and bcl­2 mRNA, and a negative correlation between bcl-2 mRNA expression and the inhibition of cell growth. In conclusion, the inhibitory effect of cisplatin on the growth of MKN45 GC cells was enhanced by rmhTNF via unknown mechanisms that involved p53ß, indicating that p53ß may be an appropriate therapeutic target for the treatment of GC.

A novel coelenterate luciferin-based luminescent probe for selective and sensitive detection of thiophenols.

The first dual bioluminescent and chemiluminescent sensor for detecting highly toxic thiophenols has been developed. Such a probe was designed by using a coelenterazine analogue as the luminophore and dinitrophenyl ether as the recognition moiety. It should be noted that this probe displayed good sensitivity and selectivity toward thiophenols, and has been effectively applied for the quantitative detection of thiophenols in aqueous media and complex biological samples.

Novel bioluminescent coelenterazine derivatives with imidazopyrazinone C-6 extended substitution for Renilla luciferase.

Two series of novel coelenterazine analogues (alkynes and triazoles) with imidazopyrazinone C-6 extended substitution have been designed and synthesized successfully for the extension of bioluminescent substrates. After extensive evaluation, some compounds display excellent bioluminescence properties compared with DeepBlueC in cellulo, thus becoming potential molecules for bioluminescence techniques.