RESUMEN
Rabies is a prevalent endemic and zoonotic fatal disease, which is normally transmitted to humans by contact (scratches and bites) from infected animals. The present paper deals with the first documented evidence of rabies in the stone marten (Martes foina). Rabies symptoms were observed in a marten in Chaharmahal and Bakhtiari province. The animal with a strange demeanour approached people without fear, which was died after some while. Samples were taken from its brain (cerebellum, hippocampus, and hypothalamus), shortly after death. In this report, laboratory evidence of rabies by fluorescent antibody (FAT) was proved. The present work is valuable because of the environmental importance of the stone marten. Hence, sensitive surveillance and advanced reporting systems should be regularly monitored on suspected cases of rabies in animals and humans to control and prevent this deadly disease. This involves exposure history, clinical examinations, symptoms and experimental results. Rabies can be controlled by fast diagnostic tests and vaccination.
Asunto(s)
Mustelidae , Rabia , Humanos , Animales , Rabia/epidemiología , Rabia/veterinaria , Irán/epidemiología , ZoonosisRESUMEN
Infectious bronchitis virus (IBV) and avian influenza virus (AIV) are two major respiratory infections in chickens. The coinfection of these viruses can cause significant financial losses and severe complications in the poultry industry across the world. To examine transcriptome profile changes during the early stages of infection, differential transcriptional profiles in tracheal tissue of three infected groups (i.e., IBV, AIV, and coinfected) were compared with the control group. Specific-pathogen-free chickens were challenged with Iranian variant-2-like IBV (IS/1494), UT-Barin isolates of H9N2 (A/chicken/Mashhad/UT-Barin/2017), and IBV-AIV coinfection; then, RNA was extracted from tracheal tissue. The Illumina RNA-sequencing (RNA-seq) technique was employed to investigate changes in the Transcriptome. Up- and downregulated differentially expressed genes (DEGs) were detected in the trachea transcriptome of all groups. The Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology databases were examined to identify possible relationships between DEGs. In the experimental groups, upregulated genes were higher compared to downregulated genes. A more severe immune response was observed in the coinfected group; further, cytokine-cytokine receptor interaction, RIG-I-like receptor signaling, Toll-like receptor signaling, NOD-like receptor signaling, Janus kinase/signal transducer, and activator of transcription, and apoptotic pathways were important upregulated genes in this group. The findings of this paper may give a better understanding of transcriptome changes in the trachea during the early stages of infection with these viruses.