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JOURNAL/nrgr/04.03/01300535-202503000-00027/figure1/v/2024-06-17T092413Z/r/image-tiff Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus, leading to long-term cognitive impairment. However, the mechanism underlying this neurogenesis impairment remains unknown. In this study, we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain injury. Our results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development, delayed neuronal maturation, and reduced the complexity of neuronal dendrites and spines. Mice with repetitive traumatic brain injuryalso showed deficits in spatial memory retrieval. Moreover, following repetitive traumatic brain injury, neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased, C1q binding protein levels were decreased, and canonical Wnt/ß-catenin signaling was downregulated. An inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological function. These findings suggest that repetitive traumatic brain injury-induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.
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The inhibitory neurons in the brain play an essential role in neural network firing patterns by releasing γ-aminobutyric acid (GABA) as the neurotransmitter. In the mouse brain, based on the protein molecular markers, inhibitory neurons are usually to be divided into three non-overlapping groups: parvalbumin (PV), neuropeptide somatostatin (SST), and vasoactive intestinal peptide (VIP)-expressing neurons. Each neuronal group exhibited unique properties in molecule, electrophysiology, circuitry, and function. Calbindin 1 (Calb1), a ubiquitous calcium-binding protein, often acts as a "divider" in excitatory neuronal classification. Based on Calb1 expression, the excitatory neurons from the same brain region can be classified into two subgroups with distinct properties. Besides excitatory neurons, Calb1 also expresses in part of inhibitory neurons. But, to date, little research focused on the intersectional relationship between inhibitory neuronal subtypes and Calb1. In this study, we genetically targeted Calb1-expression (Calb1+) and Calb1-lacking (Calb1-) subgroups of PV and SST neurons throughout the mouse brain by flexibly crossing transgenic mice relying on multi-recombinant systems, and the distribution patterns and electrophysiological properties of each subgroup were further demonstrated. Thus, this study provided novel insights and strategies into inhibitory neuronal classification.
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Encéfalo , Redes Neurales de la Computación , Animales , Ratones , Calbindina 1 , Ratones Transgénicos , Neuronas , ParvalbúminasRESUMEN
Biochar is a solid material enriched with carbon produced by the thermal transformation of organic raw materials under anoxic or anaerobic conditions. It not only has various environmental benefits including reducing greenhouse gas emissions, improving soil fertility, and sequestering atmospheric carbon, but also has the advantages of abundant precursors, low cost, and wide potential applications, thus gaining widespread attention. In recent years, researchers have been exploring new biomass precursors, improving and developing new preparation methods, and searching for more high-value and meaningful applications. Biochar has been extensively researched and utilized in many fields, and recently, it has also shown good industrial application prospects and potential application value in the printing field. In such a context, this article summarizes the typical preparation and modification methods of biochar, and also reviews its application in the printing field, to provide a reference for future work.
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In this study, 7 Pseudomonas strains were isolated from a wastewater treatment plant, and the alginate production of Pseudomonas strains under different environmental conditions was evaluated. Subsequently, alginate-biomass hydrogel beads were prepared using alginate and biomass of Pseudomonas, and their adsorption performances and mechanism to Pb2+ and Cd2+ were analyzed. The results show that weakly acidic pH and 37 °C is favorable for alginate synthesis of Pseudomonas strains, and P. alcaligenes YLS18 have the highest alginate yield (29.4 mg/g). The adsorption processes of Pb2+ and Cd2+ by hydrogel beads are well described by Langmuir model, indicating that the adsorption process is monolayer. Among the biomass of these strains, P. nitroreducens YLB32 shows the highest biosorption capacities, reaching 110.7 mg/g for Pb2+ and 54.3 mg/g for Cd2+ at pH 5. Alginate-biomass hydrogel beads obtain higher adsorption capacity to Pb2+ (184.0 mg/g) and Cd2+ (92.4 mg/g), and exhibit good reusability. The adsorption mechanism of Pb2+ and Cd2+ by hydrogel beads involves physical tapping of ions, electrostatic interactions, complexation, cation exchange and precipitation. These results provide strong support for promoting alginate recovery from activated sludge and for treating heavy metal wastewater.