METTL3-mediated N6-methyladenosine modification and HDAC5/YY1 promote IFFO1 downregulation in tumor development and chemo-resistance.

Ovarian cancer (OC) is a malignant tumor that seriously threatens women's health. Due to the difficulty of early diagnosis, most patients exhibit advanced disease or peritoneal metastasis at diagnosis. We discovered that IFFO1 is a novel tumor suppressor, but its role in tumorigenesis, development and chemoresistance is unknown. In this study, IFFO1 levels were downregulated across cancers, leading to the acceleration of tumor development, metastasis and/or cisplatin resistance. Overexpression of IFFO1 inhibited the translocation of ß-catenin to the nucleus and decreased tumor metastasis and cisplatin resistance. Furthermore, we demonstrated that IFFO1 was regulated at both the transcriptional and posttranscriptional levels. At the transcriptional level, the recruitment of HDAC5 inhibited IFFO1 expression, which is mediated by the transcription factor YY1, and the METTL3/YTHDF2 axis regulated the mRNA stability of IFFO1 in an m6A-dependent manner. Mice injected with IFFO1-overexpressing cells had lower ascites volumes and tumor weights throughout the peritoneal cavity than those injected with parental cells expressing the vector control. In conclusion, we demonstrated that IFFO1 is a novel tumor suppressor that inhibits tumor metastasis and reverses drug resistance in ovarian cancer. IFFO1 was downregulated at both the transcriptional level and posttranscriptional level by histone deacetylase and RNA methylation, respectively, and the IFFO1 signaling pathway was identified as a potential therapeutic target for cancer.

A Rare Case of Craniocervical Penetrating Injury by a Steel Bar.

RATIONALE: Non-missile penetrating injuries caused by foreign bodies, such as knives or sharp wood, are infrequent. We report a 49-year-old male suffering from severe craniocervical penetrating injury by a steel bar was successfully treated by surgery. CHIEF COMPLAINT: The male patient was a 49-year-old builder. Although working on the construction site, an approximately 60 cm steel bar penetrated the patient's brain vertically through the left top of the head presenting with unconsciousness and intermittent irritability. DIAGNOSIS: Computed tomography of the head showed the entrance and exit of the skull damaged by the steel bar. Three-dimensional reconstruction showed that the steel bar entered the skull from the posterior left coronal suture and penetrated the ipsilateral occipital bone, about 5 cm into the neck soft tissue. INTERVENTION: We successfully performed the operation and removed the steel bar. OUTCOMES: The patient was followed up for 5 years; muscle strength returned to normal. LESSONS: Penetrating injuries caused by steel bars are rare, which always cause severe intracranial injury combined with peripheral tissue injury, by sharing our experience in the treatment of this rare case, we hope to provide a reference for similar injuries in the future.

Multifunctional Wearable Sensing Devices Based on Functionalized Graphene Films for Simultaneous Monitoring of Physiological Signals and Volatile Organic Compound Biomarkers.

In this study, a multifunctional wearable sensing device based on two different graphene films is fabricated and can achieve the simultaneous detection of physiological signals and volatile organic compound (VOC) biomarkers without mutual signal interference. The wearable device was designed with two sensing components: on the upper layer of the device, four kinds of porphyrin-modified reduced graphene oxide (rGO) films were prepared and used for a sensor array that could sufficiently react with VOC vapors to achieve highly sensitive detection. A porous rGO film was designed on the underlayer of the device and used as a strain-sensing matrix, which could be closely attached to the skin to achieve a highly sensitive detection of the physiological signal. A polyimide film between the two sensing components was used not only as a flexible substrate, but also as a protective layer to avoid the porous rGO film's response to VOC molecules. Investigation of the detection ability showed that the porous rGO strain-sensing matrix can achieve a higher gauge factor (282.28) than the unstructured rGO counterpart (8.96) and is more desirable for the detection of physiological motion. In contrast, the porphyrin-modified rGO sensor array displayed a superior response to VOC vapors, and eight different VOC biomarkers could be detected and discriminated using the as-prepared sensor array together with a pattern recognition approach. The multifunctional sensing devices displayed excellent ability for the detection of a variety of human physiological signals, such as pulse and respiration rates. Simultaneous analysis of simulated diabetic breath samples, simulated nephrotic breath samples, and breath samples exhaled by healthy individuals using our wearable device exhibited clear identification and discrimination. Our study provides new insights into fabrication and design of multifunctional sensing devices without signal interference, and the application of the proposed devices are promising in preventive medicine and health care.

A multifunctional wearable sensor based on a graphene/inverse opal cellulose film for simultaneous, in situ monitoring of human motion and sweat.

A multifunctional, wearable sensor based on a reduced oxide graphene (rGO) film onto a porous inverse opal acetylcellulose (IOAC) film has been developed and can perform simultaneous, in situ monitoring of various human motions and ion concentrations in sweat. The rGO film is used as a strain-sensing layer for monitoring human motion via its resistance change, whereas the porous IOAC film is used as a flexible microstructured substrate not only for high sensitive motion sensing, but also for collection and analysis of ion concentrations in sweat by its simple colorimetric changes or reflection-peak shifts. Studies on humans demonstrated that the devices have excellent capability for monitoring various human motions, such as finger bending motion, wrist bending motion, head rotation motion and various small-scale motions of the throat. Simultaneous, in situ analysis of the ion concentration in sweat during these motions shows that the IOAC substrate can detect a wide range of NaCl concentrations in sweat from normal 30 to 680 mM under the conditions of severe dehydration. This investigation provides new horizons toward the design and fabrication of multifunctional, wearable health monitoring devices and the proposed wearable sensor shows promising applications in healthcare and preventive medicine.