Yeast culture enhances long-term fermentation of corn straw by ruminal microbes for volatile fatty acid production: Performance and mechanism.

Ruminal microbes can efficiently ferment biomass waste to produce volatile fatty acids (VFAs). However, keeping long-term efficient VFA production efficiency has become a bottleneck. In this study, yeast culture (YC) was used to enhance the VFA production in long-term fermentation. Results showed that YC group improved the volatile solid removal and VFA concentration to 47.8% and 7.82 g/L, respectively, 18.6% and 16.1% higher than the control, mainly enhancing the acetic, propionic, and butyric acid production. YC addition reduced the bacterial diversity, changed the bacterial composition, and improved interactions among bacteria. The regulation mechanism of YC was to increase the abundance and activity of hydrolytic and acidogenic bacteria such as Prevotella and Treponema, improve bacterial interactions, and further promote expression of functional genes. Ultimately, a long-term efficient ruminal fermentation of corn straw into VFAs was achieved.

Recent advances in methane and hydrogen production from lignocellulosic degradation with anaerobic fungi.

Anaerobic fungi (AF) efficiently degrade lignocellulosic biomass with unique pseudoroot system and enzymatic properties that can remove polysaccharides and some lignified components from plant cell walls, further releasing acetate, lactate, ethanol, hydrogen (H2), etc. As research on AF for bioengineering has become a hot topic, a review of lignocellulosic conversion with AF for methane (CH4) and H2 production is needed. Efficient degradation of lignocellulose with AF mainly relies on multiple free carbohydrate-active enzymes and cellulosomes in the free and bound state. Meanwhile, co-cultivation of AF and methanogens significantly improves the lignocellulose degradation and CH4 production, and the maximum CH4 yield reached 315 mL/g. Bioaugmentation of AF in anaerobic digestion increases the maximum CH4 yield by 330 %. Also, AF show H2 production potential, however, H2 yield from anaerobic fungal fermentation of lignocellulose remains low. Therefore, anaerobic fungi have great potential in the conversion of lignocellulosic biomass to CH4 and H2.

A novel strategy for waste activated sludge treatment: Recovery of structural extracellular polymeric substances and fermentative production of volatile fatty acids.

Structural extracellular polymeric substances (SEPS) as valuable biopolymers, can be extracted from waste activated sludge (WAS). However, the extraction yield is typically low, and detailed information on SEPS characterizations, as well as proper treatment of the sludge after SEPS extraction, remains limited. This study aimed to optimize the conditions of heating-Na2CO3 extraction process to increase the yield of SEPS extracted from WAS. Subsequently, SEPS were characterized, and, for the first time, insights into their protein composition were uncovered by using proteomics. A maximum SEPS yield of 209 mg g-1 volatile solid (VS) was obtained under optimal conditions: temperature of 90 °C, heating time of 60 min, Na+ dosage of 8.0 mmol/g VS, and pH required to precipitation of 4.0, which was comparable to that from the aerobic granular sludge reported in literature. Proteomics analysis unveiled that the proteins in SEPS primarily originated from microorganisms involved in nitrogen fixation and organic matter degradation, including their intracellular and membrane-associated regions. These proteins exhibited various catalytic activities and played crucial roles in aggregation processes. Besides, the process of SEPS extraction significantly enhanced volatile fatty acid (VFA) production during the anaerobic fermentation of residual WAS after SEPS extraction. A maximum VFA yield of 420 ± 14 mg COD/g VSadded was observed in anaerobic fermentation of 10 d, which was 77.2 ± 0.1 % higher than that from raw sludge. Mechanism analysis revealed that SEPS extraction not only improved WAS disintegration and solubilization but also reduced the relative activity of methanogens during anaerobic fermentation. Moreover, SEPS extraction shifted the microbial population during anaerobic fermentation in the direction towards hydrolysis and acidification such as Fermentimonas sp. and Soehngenia sp. This study proposed a novel strategy based on SEPS extraction and VFA production for sludge treatment, offering potential benefits for resource recovery and improved process efficiency.

Genome-centric metagenomic analysis reveals mechanisms of quorum sensing promoting anaerobic digestion under sulfide stress: Syntrophic metabolism and microbial self-adaptation.

Sulfide stress is a common inhibition factor in anaerobic digestion systems with sulfur-rich feedstocks. Quorum sensing (QS) signaling molecule N-acyl-homoserine lactones (AHLs) possess positive effect on promoting anaerobic digestion. However, the micro-biological mechanisms of AHLs affecting syntrophic metabolism and microbial self-adaptation have not yet been deciphered in anaerobic digestion under sulfide stress. In this study, the CH4 production increased by 21.34 % at 20 µM AHLs addition in anaerobic digestion under sulfide stress. AHLs contributed to establishing potential syntrophic relationship between acidifying bacteria (unclassified_o__Bacteroidales, Lentimicrobium, Acetoanaerobium, Longilinea, and Sphaerochaetaa) and Methanothrix. AHLs promoted syntrophic metabolism by boosting microbial metabolic activity and interspecies electron transfer (IET) process under sulfide stress. For microbial metabolic activity, AHLs promoted the key enzyme synthesis in acidogenesis and methanogenesis. For IET process, AHLs promoted the assembly and synthesis of conductive pili, and synthesis and secretion of riboflavin. Furthermore, AHLs promoted microbial self-adaptation including two component system, lipopolysaccharide biosynthesis, and DNA repair, which were important evidences that microbial resistance to sulfide stress was enhanced by AHLs. Microbial self-adaptation provided favorable foundation and safeguard for syntrophic metabolisms under sulfide stress. These findings deciphered the micro-biological mechanisms of AHLs enhancing anaerobic digestion under sulfide stress.

Life cycle assessment of n-caproic acid production via chain elongation from food waste: Comparison of shunting and staged technology.

n-Caproic acid is a widely used biochemical that can be produced from organic waste through chain elongation technology. This study aims to evaluate the environmental impacts of n-caproic acid production through chain elongation by two processes (i.e., shunting and staged technology). The Open-life cycle assessment (LCA) model was used to calculate the environmental impacts of both technologies based on experimental data. Results showed that the shunting technology had higher environmental impacts than the staged technology. Water and electricity made bigger contribution to the environmental impacts of both technologies. Reusing chain elongation effluent substituting for water and using electricity produced by wind power could reduce the environmental impacts of water and electricity effectively. Using ethanol from food waste had higher global warming potential than fossil ethanol, which suggested that a cradle-to-grave LCA is needed to be carried out for specific raw materials and chain elongation products in the future.

Insights into the influence of polystyrene microplastics on the bio-degradation behavior of tetrabromobisphenol A in soil.

Soil contamination by emerging pollutants tetrabromobisphenol A (TBBPA) and microplastics has become a global environmental issue in recent years. However, little is known about the effect of microplastics on degradation of TBBPA in soil, especially aged microplastics. In this study, the effect of aged polystyrene (PS) microplastics on the degradation of TBBPA in soil and the mechanisms were investigated. The results suggested that the aged microplastics exhibited a stronger inhibitory effect on the degradation of TBBPA in soil than the pristine microplastics, and the degradation efficiency of TBBPA decreased by 21.57% at the aged microplastic content of 1%. This might be related to the higher TBBPA adsorption capacity of aged microplastics compared to pristine microplastics. Aged microplastics strongly altered TBBPA-contaminated soil properties, reduced oxidoreductase activity and affected microbial community composition. The decrease in soil oxidoreductase activity and relative abundance of functional microorganisms (e.g., Bacillus, Pseudarthrobacter and Sphingomonas) caused by aged microplastics interfered with metabolic pathways of TBBPA. This study indicated the importance the risk assessment and soil remediation for TBBPA-contaminated soil with aged microplastics.

Effect of high corn straw loads on short-chain fatty acid production in semi-continuous rumen reactor.

Ruminal microorganisms can efficiently hydrolyze biomass waste for short-chain fatty acid (SCFA) production. However, the continuous SCFA production by ruminal microorganisms at high loads is unclear. In this study, the effectiveness of a rumen semi-continuous reactor at high load for SCFA production was explored. Results showed that SCFA concentration reached 13.3 g/L at 8 % (w/v) corn straw load. The higher the corn straw load, the lower the volatile solid removal. Rumen microbial community composition changed significantly with increasing corn straw load. A significant decrease in bacterial diversity and abundance was observed at 8 % corn straw load. Some core genera such as Prevotella, Saccharofermentans, and Ruminococcus significantly increased. As corn straw loads increased, the expression of functional genes related to hydrolysis and acidogenesis gradually increased. Thus, the 8.0 % load is suitable for SCFA production. These findings provide new insights into high load fermentation of ruminal microorganisms.

Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review.

The rumen of ruminants is a natural anaerobic fermentation system that efficiently degrades lignocellulosic biomass and mainly depends on synergistic interactions between multiple microbes and their secreted enzymes. Ruminal microbes have been employed as biomass waste converters and are receiving increasing attention because of their degradation performance. To explore the application of ruminal microbes and their secreted enzymes in biomass waste, a comprehensive understanding of these processes is required. Based on the degradation capacity and mechanism of ruminal microbes and their secreted lignocellulose enzymes, this review concentrates on elucidating the main enzymatic strategies that ruminal microbes use for lignocellulose degradation, focusing mainly on polysaccharide metabolism-related gene loci and cellulosomes. Hydrolysis, acidification, methanogenesis, interspecific H2 transfer, and urea cycling in ruminal metabolism are also discussed. Finally, we review the research progress on the conversion of biomass waste into biofuels (bioethanol, biohydrogen, and biomethane) and value-added chemicals (organic acids) by ruminal microbes. This review aims to provide new ideas and methods for ruminal microbe and enzyme applications, biomass waste conversion, and global energy shortage alleviation.

Bioconversion of volatile fatty acids from organic wastes to produce high-value products by photosynthetic bacteria: A review.

Anaerobic fermentation of organic waste to produce volatile fatty acids (VFAs) production is a relatively mature technology. VFAs can be used as a cheap and readily available carbon source by photosynthetic bacteria (PSB) to produce high value-added products, which are widely used in various applications. To better enhance the VFAs obtained from organic wastes for PSB to produce high value-added products, a comprehensive review is needed, which is currently not available. This review systematically summarizes the current status of microbial proteins, H2, poly-ß-hydroxybutyrate (PHB), coenzyme Q10 (CoQ10), and 5-aminolevulinic acid (ALA) production by PSB utilizing VFAs as a carbon resource. Meanwhile, the metabolic pathways involved in the H2, PHB, CoQ10, and 5-ALA production by PSB were deeply explored. In addition, a systematic resource utilization pathway for PSB utilizing VFAs from anaerobic fermentation of organic wastes to produce high value-added products was proposed. Finally, the current challenges and priorities for future research were presented, such as the screening of efficient PSB strains, conducting large-scale experiments, high-value product separation, recovery, and purification, and the mining of metabolic pathways for the VFA utilization to generate high value-added products by PSB.

Response of rumen microorganisms to pH during anaerobic hydrolysis and acidogenesis of lignocellulose biomass.

Rumen microorganisms can efficiently degrade lignocellulosic wastes to produce volatile fatty acids (VFAs). pH is a key factor in controlling the type and yield of VFAs by affecting the microorganisms involved in rumen fermentation. However, the effects of different pH on rumen microbial diversity, communities, and mechanisms are unclear. In this study, the hydrolysis and acidogenesis of corn straw and diversity, communities, and mechanisms of rumen microorganisms were explored at different initial pHs. Results showed that the highest hemicellulose, cellulose, and lignin degradation efficiency of corn straw was 55.2 %, 38.3 %, and 7.01 %, respectively, and VFA concentration was 10.2 g/L at pH 7.0. Low pH decreased the bacterial diversity and increased the fungal diversity. Rumen bacteria and fungi had different responses to initial pHs, and the community structure of bacteria and fungi had obviously differences at the genus level. The core genera Succiniclasticum, Treponema, and Neocallimastix relative abundance at initial pH 7.0 samples were significantly higher than that at lower initial pHs, reaching 6.01 %, 1.61 %, and 5.35 %, respectively. The bacterial network was more complex than that of fungi. pH, acetic acid, and propionic acid were the main factors influencing the bacterial and fungal community structure. Low pH inhibited the expression of functional genes related to hydrolysis and acidogenesis, explaining the lower hydrolysis and acidogenesis efficiency. These findings will provide a better understanding for rumen fermentation to produce VFAs.

Prediction of carbon emissions peak and carbon neutrality based on life cycle CO2 emissions in megacity building sector: Dynamic scenario simulations of Beijing.

In order to design an optimal carbon peak and carbon neutralization pathway for the high-density building sector, a dynamic prediction model is established using system-dynamics coupled building life cycle carbon emission model (SD-BLCA) with consideration of future evolutionary trajectory and time constraints. The model is applied in Beijing using the SD-BLCA combined with scenario analysis and Monte Carlo methods to explore optimal trajectory for its building sector under 30-year timeframe. The results indicate that by increasing the proportion of renewable energy generation by 7% and retrofitting 60 million m2 of existing buildings, these two mature measures can offset the growth of carbon emissions and achieve the peak target by 2025. However, achieving carbon neutrality necessitates a shift from isolated technologies to a comprehensive net-zero emissions strategy. The study proposes a time roadmap that integrates a zero-carbon energy supply system and the carbon reduction measures of the whole life cycle. This strategy primarily relies on renewable sources to provide heat, power, and hydrogen, resulting in estimated reductions of 29.8 Mt, 28.1 Mt, and 0.7 Mt, respectively. Zero energy buildings, green buildings, and renovated buildings can reduce carbon emissions through their own energy-saving measures by 8.4, 18.2, and 11.8 kg/m2, respectively.

Advances in understanding entire process of medium chain carboxylic acid production from organic wastes via chain elongation.

Chain elongation is an environmentally friendly biological technology capable of converting organic wastes into medium chain carboxylic acids (MCCAs). This review aims to offer a comprehensive analysis of MCCA production from organic wastes via chain elongation. Seven kinds of organic wastes are introduced and classified as easily degradable and hardly degradable. Among them, food waste, fruit and vegetable waste are the most potential organic wastes for MCCA production. Combined pretreatment technologies should be encouraged for the pretreatment of hardly degradable organic wastes. Furthermore, the mechanisms during MCCA production are analyzed, and the key influencing factors are evaluated, which affect the MCCA production and chain elongation efficiency indirectly. Extracting MCCA simultaneously is the most important way to improve MCCA production efficiency, and technologies for sequentially extracting different kinds of MCCAs are recommended. Finally, some perspectives for future chain elongation researches are proposed to promote the large-scale application of chain elongation.

Enhanced thyroxine removal from micro-polluted drinking water resources in a bio-electrochemical reactor amended with TiO2@GAC particles: Efficiency, mechanism and energy consumption.

A three-dimensional bioelectrochemical system (3D-BES) with both electrocatalytic and biodegradation functions was designed and developed to enhance iodine-containing hormone removal from micro-polluted oligotrophic drinking water sources and to reduce energy consumption. Thyroxine (T4) removal efficiency was 99.0% in the 3D-BES amendment with TiO2@GAC as the particle electrodes, which was 20.5% higher than the total efficiency of single biodegradation (28.7%) plus electrochemical decomposition (49.8%). The high T4 removal efficiency was a result of biochemical synergistic degradation, enhancement of electron transfer and utilization, enrichment of functional microorganisms, and the expression of dehalogenation functional genes. The electron transfer was increased by 1.63 times in 3D-BES compared to the 2D-BES, which contributed to: (i) ∼17.8% enhancement of dehalogenation, (ii) 2.35 times enhancement of the attenuation rate, and (iii) 60% reduction in energy consumption. Moreover, the aggregation of microorganisms and the hydrophobic T4 onto TiO2@GAC shortened the transfer distance of matter and energy, which induced the degradation steps to be shortened and the toxic decay to be accelerated from T4 and its metabolites. These comprehensive functions also enhanced the 31.8% ATPase activity, 7.3% abundance of the functional reductive dehalogenation genera, and 52.3% dehalogenation genes expression for Pseudomonas, Ancylobacter, and Dehalogenimonas, which contributed to an increase in T4 removal. This work provides an environmental-friendly biochemical synergistic method for the detoxification of T4 polluted water.

Adsorption behaviors and bioavailability of tetrabromobisphenol A in the presence of polystyrene microplastic in soil: Effect of microplastics aging.

Microplastics, a kind of emerging pollutant, have become a global environmental research hotspot in recent years due to its wide distribution in soil and its impact on soil ecosystems. However, little information is available on the interactions between microplastics and organic contaminants in soil, especially after microplastic aging. The impact of polystyrene (PS) microplastic aging on the sorption of tetrabromobisphenol A (TBBPA) in soil and the desorption characteristics of TBBPA-loaded microplastics in different environments were studied. The results showed a significant increase of 76.3% in adsorption capacity of TBBPA onto PS microplastics after aging for 96 h. Based on the results of characterization analysis and density functional theory (DFT) calculation, the mechanisms of TBBPA adsorption changed mainly from hydrophobic and π-π interactions on pristine PS microplastics to hydrogen bond and π-π interactions on aged PS microplastics. The presence of PS microplastics increased the TBBPA sorption capacity onto soil-PS microplastics system and significantly altered the distribution of TBBPA on soil particles and PS microplastics. The high TBBPA desorption over 50% from aged PS microplastics in simulated earthworm gut environment suggested that TBBPA contamination combined with PS microplastics might pose a higher risk to macroinvertebrates in soil. Overall, these findings contribute to the understanding of impact of PS microplastic aging in soil on the environmental behaviors of TBBPA, and provide valuable reference for evaluating the potential risk posed by the co-existence of microplastics with organic contaminants in soil ecosystems.

How does water diversion affect land use change and ecosystem service: A case study of Baiyangdian wetland, China.

Baiyangdian wetland is the biggest plant-dominated shallow freshwater wetland in Huabei Plain, providing a wide range of ecosystem services. In the past few decades, the water scarcity and eco-environmental problems resulted from climate changes and human activities have become more and more serious. To relieve the pressure of water scarcity and ecological degradation, the government has implemented ecological water diversion projects (EWDPs) since 1992. In this study, land use and land cover change (LUCC) caused by EWDPs over three decades was analyzed to quantitatively assess the impact of EWDPs on ecosystem services. Coefficients of ecosystem service value (ESV) calculation were improved for regional ESV evaluation. The results showed that the area of construction, farmland and water increased by 6171, 2827, 1393 ha, respectively, and the total ESV increased by 8.04 × 108 CNY primarily due to the increase of regulating service with water area expansion. Redundancy analysis and socio-economic comprehensive analysis showed that EWDPs impacted water area and ESV with threshold and time effect. When the water diversion exceeded the threshold, the EWDPs affected the ESV through influencing LUCC; otherwise, the EWDPs affected the ESV through influencing net primary productivity or social-economic benefits. However, the impact of EWDPs on ESV gradually weakened as time passed, which could not keep sustainability. With the establishment of Xiong'an New Area in China and implementation of carbon neutrality policy, rational EWDPs will become crucial to achieve goals of ecological restoration.

Advanced treatment and valorization of food waste through staged fermentation and chain elongation.

A novel valorization approach of food waste via staged fermentation and chain elongation was proposed. Food waste was moderately saccharified, saccharification effluent was fermented to produce ethanol and saccharification residue was hydrolyzed and acidified to produce VFAs. The yeast fermentation effluent and hydrolytic acidification effluent were sequentially performed for chain elongation. Ethanol and volatile fatty acids from staged fermentation were suitable for direct chain elongation and the n-caproate production was 184.69 mg COD/g VS when yeast fermentation effluent to hydrolytic acidification effluent ratio was 2:1. Food waste was deeply utilized with an organic conversion of 80%. The relative abundance of Clostridium sensu stricto increased during chain elongation, which might be responsible for the improvement of n-caproate production. A profit of 10.65 USD/t was estimated for chain elongation of food waste staged fermentation effluent. This study provided a new technology to achieve advanced treatment and high-valued utilization of food waste.

Metagenomic analysis reveals the efficient digestion mechanism of corn stover in Angus bull rumen: Microbial community succession, CAZyme composition and functional gene expression.

Ruminant rumen is a biological fermentation system that can efficiently degrade lignocellulosic biomass. The knowledge about mechanisms of efficient lignocellulose degradation with rumen microorganisms is still limited. In this study, composition and succession of bacteria and fungi, carbohydrate-active enzymes (CAZymes), and functional genes involved in hydrolysis and acidogenesis were revealed during fermentation in Angus bull rumen via metagenomic sequencing. Results showed that degradation efficiency of hemicellulose and cellulose reached 61.2% and 50.4% at 72 h fermentation, respectively. Main bacterial genera were composed of Prevotella, Butyrivibrio, Ruminococcus, Eubacterium, and Fibrobacter, and main fungal genera were composed of Piromyces, Neocallimastix, Anaeromyces, Aspergillus, and Orpinomyces. Principal coordinates analysis indicated that community structure of bacteria and fungi dynamically changed during 72 h fermentation. Bacterial networks with higher complexity had stronger stability than fungal networks. Most CAZyme families showed a significant decrease trend after 48 h fermentation. Functional genes related to hydrolysis decreased at 72 h, while functional genes involved in acidogenesis did not change significantly. These findings provide a in-depth understanding of mechanisms of lignocellulose degradation in Angus bull rumen, and may guide the construction and enrichment of rumen microorganisms in anaerobic fermentation of waste biomass.

Volatile fatty acid production in anaerobic fermentation of food waste saccharified residue: Effect of substrate concentration.

In this study, food waste saccharified residue was used to produce volatile fatty acids (VFAs), and the effects of substrate concentration on VFA production, VFA composition, acidogenic efficiency, microbial community, and carbon transfer were investigated. Interestingly, chain elongation from acetate to n-butyrate played an important role with a substrate concentration of 200 g/L in the acidogenesis process. Results showed that 200 g/L was a suitable substrate concentration for both VFA and n-butyrate production, the highest VFA production, and n-butyrate composition were 280.87 mg COD/g vS and more than 90.00 %, respectively, and VFA/SCOD reached 82.39 %. Microbial analysis showed that Clostridium_Sensu_Stricto_12 promoted n-butyrate production by chain elongation. Carbon transfer analysis indicated that chain elongation made a contribution of 43.93 % to n-butyrate production. Totally 38.47 % of organic matter in food waste saccharified residue was further utilized. This study provides a new way for n-butyrate production with waste recycling and low cost.

Detoxification of fluoroglucocorticoid by Acinetobacter pittii C3 via a novel defluorination pathway with hydrolysis, oxidation and reduction: Performance, genomic characteristics, and mechanism.

Biological dehalogenation degradation was an important detoxification method for the ecotoxicity and teratogenic toxicity of fluorocorticosteroids (FGCs). The functional strain Acinetobacter pittii C3 can effectively biodegrade and defluorinate to 1 mg/L Triamcinolone acetonide (TA), a representative FGCs, with 86 % and 79 % removal proportion in 168 h with the biodegradation and detoxification kinetic constant of 0.031/h and 0.016/h. The dehalogenation and degradation ability of strain C3 was related to its dehalogenation genomic characteristics, which manifested in the functional gene expression of dehalogenation, degradation, and toxicity tolerance. Three detoxification mechanisms were positively correlated with defluorination pathways through hydrolysis, oxidation, and reduction, which were regulated by the expression of the haloacid dehalogenase (HAD) gene (mupP, yrfG, and gph), oxygenase gene (dmpA and catA), and reductase gene (nrdAB and TgnAB). Hydrolysis defluorination was the most critical way for TA detoxification metabolism, which could rapidly generate low-toxicity metabolites and reduce toxic bioaccumulation due to hydrolytic dehalogenase-induced defluorination. The mechanism of hydrolytic defluorination was that the active pocket of hydrolytic dehalogenase was matched well with the spatial structure of TA under the adjustment of the hydrogen bond, and thus induced molecular recognition to promote the catalytic hydrolytic degradation of various amino acid residues. This work provided an effective bioremediation method and mechanism for improving defluorination and detoxification performance.

Quorum quenching enhanced methane production in anaerobic systems - performance and mechanisms.

In our previous study, quorum quenching (QQ) bacteria can effectively enhance methane production in an anaerobic membrane bioreactor (AnMBR) while mitigating membrane biofouling. However, the mechanism of such enhancement is unclear. In this study, we analyzed the potential effects from separated hydrolysis, acidogenesis, acetogenesis and methanogenesis steps. The cumulative methane production improved by 26.13%, 22.54%, 48.70% and 44.93% at QQ bacteria dosage of 0.5, 1, 5 and 10 mg strain/g beads, respectively. It was found that the presence of QQ bacteria enhanced acidogenesis step resulting in higher volatile fatty acids (VFA) production, while it had no obvious influence on hydrolysis, acetogenesis and methanogenesis steps. The substrate (glucose) conversion efficiency in acidogenesis step was also accelerated (1.45 folds vs control within first eight hours). The abundance of hydrolytic fermentation gram-positive bacteria and several acidogenic bacteria, such as Hungateiclostridiaceae, was promoted in QQ amended culture, which enhanced VFA production and accumulation. Although the abundance of acetoclastic methanogen Methanosaeta reduced by 54.2% on the 1st day of QQ beads addition, the overall performance of methane production was not affected. This study revealed that QQ had a greater impact on the acidogenesis step in the anaerobic digestion process, though the microbial community in acetogenesis and methanogenesis steps was altered. This work can provide a theoretical basis for using QQ technology to slow down the rate of membrane biofouling in anaerobic membrane bioreactors while increasing methane production and maximizing economic benefits.