Acetyl CoA synthase 2 potentiates ATG5-induced autophagy against neuronal apoptosis after subarachnoid hemorrhage.

ATG5-induced autophagy is triggered in the early stages after SAH, which plays a vital role in subarachnoid hemorrhage (SAH). Acyl-CoA synthetase short-chain family 2 (ACSS2) is not just involved in energy metabolism but also binds to TEFB to form a complex translocated to related autophagy genes to regulate the expression of autophagy-related genes. However, the contribution of ACSS2 to the activation of autophagy in early brain injury (EBI) after SAH has barely been discussed. The purpose of this study was to investigate the alterations of ACSS2 and its neuroprotective effects following SAH. We first evaluated the expression of ACSS2 at different time points (6, 12, 24, and 72 h after SAH) in vivo and primary cortical neurons stimulated by oxyhemoglobin (OxyHb). Subsequently, adeno-associated virus and lentivirus were used to regulate ACSS2 expression to investigate the effect of ACSS2 after SAH. The results showed that the ACSS2 level decreased significantly in the early stages of SAH and was minimized at 24 h post-SAH. After artificial intervention to overexpress ACSS2, ATG5-induced autophagy was further enhanced in EBI after SAH, and neuronal apoptosis was alleviated to protect brain injury. In addition, brain edema and neurological function scores were improved. These results suggest that ACSS2 plays an important role in the neuroprotection against EBI after SAH by increasing ATG5-induce autophagy and inhibiting apoptosis.

Correlation Between Vascular Geometry Changes and Long-Term Outcomes After Enterprise Stent Deployment for Intracranial Aneurysms Located on Small Arteries.

BACKGROUND: Enterprise stents are widely used for intracranial aneurysms located on small arteries (<2.5 mm in diameter) and change the geometry of parent arteries. The purpose of this study was to investigate the correlation between vascular geometry changes and long-term outcomes. METHODS: Between May 2013 and 2018, 1065 consecutive intracranial aneurysms were treated with Enterprise stents at our institution. After inclusion and exclusion criteria were applied, 377 aneurysms with >6 months of digital subtraction angiography follow-up were evaluated. The cohort comprised 101 aneurysms located on small parent arteries. After stent-assisted coiling, the vascular geometry parameters of small parent arteries were compared to explore their correlation with procedural complications, delayed stent migration, and recanalization. RESULTS: The rate of delayed aneurysm occlusion in patients with initial efferent artery diameter (De) <2.5 mm was significantly higher than in patients with De >2.5 mm (62.2% vs. 40.2%; P = 0.032). At follow-up, vascular geometry parameters significantly increased (P < 0.001). In multivariate analyses, larger aneurysms and initial parent artery angle (α) <90° were independent predictors of procedural complications and discrepancy in vessel size (ΔD) >0.5 mm was an independent predictor of delayed stent migration. Larger aneurysms and follow-up angle change (ΔAngle) <30° were independent predictors for recanalization of aneurysms located on small arteries. CONCLUSIONS: Enterprise stent-assisted coiling of intracranial aneurysms located on small arteries is safe and effective. Our study found that Enterprise deployment in small arteries had a low procedural complication rate and high stent tolerance. Vascular geometry changes play an important role in aneurysm recanalization.

Electrochemical study of enhanced nitrate removal in wastewater treatment using biofilm electrode.

Bioelectrochemical enhanced nitrate removal in wastewater with high total nitrogen and low organic carbon was electrochemically investigated focusing on the relationship between biochemical and electrochemical nitrogen cycles. Under optimized external voltage of -0.6 V, apparent nitrate removal rate of bioelectrochemical denitrification was 76% higher than normal biofilm denitrification. And with the introduction of biofilm on the electrode, new reduction peak of N2O, much larger current density, and 0.4 V positively shift of on-set potential of nitrate reduction reaction were observed, suggesting a synergy of electrochemical reaction and biological reaction through enhanced electrochemical reduction of intermediate products from biological process. Oxygen reduction reaction could not be avoided during nitrogen electrochemical reduction reaction since their similar reduction potential. But it led to decrease of oxygen concentration and therefore contribute to biological denitrification. Bacteria community tests also supported a dominant bacteria which could denitrify and use external electron.