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The printing and dyeing industry is currently accelerating toward a direction of high efficiency, energy conservation, environmental protection, and integration with digitalization. Disperse dye wash-free digital inkjet dyeing is a revolutionary breakthrough for cleaning and coloring polyester fabric. Based on the solubility parameters and the hot-melt dyeing characteristics of disperse dyes, soft, hard, and functional monomers of acrylate were used as the main body. Moreover, single-vinyl fluorinated polysiloxane and divinyl polysiloxane with low solubility parameters were used as modified monomers. A modified polyacrylate (PFSMA) adhesive containing silicon in the main chain and fluorine silicon in the side chain was prepared via miniemulsion polymerization. Using disperse digital inkjet dyeing of polyester fabric without washing can realize energy saving, emission reduction, and carbon reduction. Results showed that the optimum preparation conditions of PFSMA were as follows: DVFS molecular weight of 957 g/mol and DVFS content of 2.5 wt %. Compared with that of polyacrylate (PA), the glass-transition temperature of PFSMA film decreased, and its water resistance, toughness, and adhesion enhanced. When the PFSMA content in the wash-free disperse red ink was 8 wt %, the color yields of the front and back of the PFSMA jet-dyed polyester fabric were 18.86 and 13.28, respectively. Moreover, the color yield of the front of PFSMA jet-dyed polyester fabric was 39.9% higher than that of the pure liquid disperse red jet-dyed fabric. The simulated fixation rate was 87.9%, approximately 2.9 times higher than that of the PA wash-free jet-dyed fabric. The color fastness to dry rubbing reached level 4 and the color fastness to wet rubbing reached level 3-4, which was one level higher than that of pure liquid disperse red jet-dyed fabrics. The color fastness to soaping reached grade 5 and the color fastness to heat compression reached grades 4-5 and above. The fabric was a little firmer but smoother. The color properties, color fastness, and hand feeling of the PFSMA wash-free jet-dyed polyester fabric exceeded the levels of commercially available adhesives.
RESUMEN
BACKGROUND: To explore the neuroprotective mechanism of artemisinin against ischemic stroke from the perspective of NLRP3-mediated pyroptosis. METHODS: Serum metabolomics technology was used to analyze the serum samples of mice, and KEGG metabolic pathway was analyzed for the different metabolites in the samples. PIT model and OGD/R model were used to simulate ischemic stroke damage in vivo and in vitro. Hoechst 33342 staining, Annexin V-FITC/PI staining and TUNEL staining were used to detect the pyroptosis rate of cells. The contents of IL-1ß and IL-18 in PC12 cells and serum of mice were detected by ELISA. The expressions of NLRP3, ASC-1, Caspase-1 and TXNIP in PC12 cells and mouse brain tissue were detected by Western Blot. RESULTS: Serum metabolic profiles of animal models identified 234 different metabolites and 91 metabolic pathways. Compared with the Sham group and the Stroke+ART group, the KEGG pathway in the Stroke group was concentrated in the Necroptosis pathway associated with cell growth and death, and the NLRP3 inflammasome-mediated pyroptosis pathway was activated in the Necroptosis pathway after ischemic stroke. The results of in vivo and in vitro experiments showed that pretreatment with 10⯵M artemisinin reduced ROS production, decreased Δψm, reduced pyroptosis, maintained neuronal cell morphology, and down-regulated the contents of IL-1ß and IL-18 as well as the expression of key proteins of NLRP3, ASC-1, Caspase-1 and TXNIP(p<0.01). CONCLUSION: Artemisinin can reduce neuronal pyroptosis induced by ischemic stroke by inhibiting ROS/TXNIP/NLRP3/Caspase-1 signaling pathway.
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Artemisininas , Proteínas Portadoras , Caspasa 1 , Accidente Cerebrovascular Isquémico , Ratones Endogámicos C57BL , Proteína con Dominio Pirina 3 de la Familia NLR , Piroptosis , Especies Reactivas de Oxígeno , Transducción de Señal , Animales , Piroptosis/efectos de los fármacos , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Caspasa 1/metabolismo , Transducción de Señal/efectos de los fármacos , Accidente Cerebrovascular Isquémico/tratamiento farmacológico , Accidente Cerebrovascular Isquémico/metabolismo , Accidente Cerebrovascular Isquémico/patología , Artemisininas/farmacología , Ratones , Proteínas Portadoras/metabolismo , Células PC12 , Especies Reactivas de Oxígeno/metabolismo , Masculino , Ratas , Proteínas de Ciclo Celular/metabolismo , Fármacos Neuroprotectores/farmacología , Inflamasomas/metabolismo , Inflamasomas/efectos de los fármacos , Modelos Animales de Enfermedad , TiorredoxinasRESUMEN
Programmed death ligand-1 (PD-L1)-expressing exosomes are considered a potential marker for diagnosis and classification of lung adenocarcinoma (LUAD). There is an urgent need to develop highly sensitive and accurate chemiluminescence (CL) immunosensors for the detection of PD-L1-expressing exosomes. Herein, N-(4-aminobutyl)-N-ethylisopropanol-functionalized nickel-cobalt hydroxide (NiCo-DH-AA) with a hollow nanoflower structure as a highly efficient CL nanoprobe was synthesized using gold nanoparticles as a "bridge". The resulting NiCo-DH-AA exhibited a strong and stable CL emission, which was ascribed to the exceptional catalytic capability and large specific surface area of NiCo-DH, along with the capacity of AuNPs to facilitate free radical generation. On this basis, an ultrasensitive sandwich CL immunosensor for the detection of PD-L1-expressing exosomes was constructed by using PD-L1 antibody-modified NiCo-DH-AA as an effective signal probe and rabbit anti-CD63 protein polyclonal antibody-modified carboxylated magnetic bead as a capture platform. The immunosensor demonstrated outstanding analytical performance with a wide detection range of 4.75 × 103-4.75 × 108 particles/mL and a low detection limit of 7.76 × 102 particles/mL, which was over 2 orders of magnitude lower than the reported CL method for detecting PD-L1-expressing exosomes. Importantly, it was able to differentiate well not only between healthy persons and LUAD patients (100% specificity and 87.5% sensitivity) but also between patients with minimally invasive adenocarcinoma and invasive adenocarcinoma (92.3% specificity and 52.6% sensitivity). Therefore, this study not only presents an ultrasensitive and accurate diagnostic method for LUAD but also offers a novel, simple, and noninvasive approach for the classification of LUAD.
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Adenocarcinoma del Pulmón , Antígeno B7-H1 , Cobalto , Exosomas , Neoplasias Pulmonares , Níquel , Humanos , Níquel/química , Cobalto/química , Antígeno B7-H1/análisis , Adenocarcinoma del Pulmón/diagnóstico , Adenocarcinoma del Pulmón/inmunología , Neoplasias Pulmonares/diagnóstico , Exosomas/química , Inmunoensayo/métodos , Hidróxidos/química , Nanopartículas del Metal/química , Técnicas Biosensibles/métodos , Oro/química , Mediciones Luminiscentes/métodos , Límite de DetecciónRESUMEN
It has become a growing trend for the development of antibacterial fabrics of high effectiveness and durability without affecting their intrinsic wearability. Herein, a new antibacterial agent (AgNPs@HTCS) was prepared by grafting of natural hinokitiol (HT) onto chitosan (CS) via Mannich reaction, and then coordination of nanosilver (AgNPs) via in-situ reduction. AgNPs@HTCS was applied for the construction of durable antibacterial fabrics. Results showed that the minimum inhibitory concentration values of AgNPs@HTCS against S. aureus and E. coli reached 1.74 µg/mL and 5.28 µg/mL, respectively. AgNPs@HTCS solution at very low concentration of 0.25 g/L could impart antibacterial ratio above 99% against S. aureus and E. coli for cotton, silk, linen, and polyester fabrics. After 25 repeated washing recycles, the antibacterial ratios of the treated fabrics still retained higher than 95%. Compared with the untreated fabric, the handle, whiteness, and breaking strength of the antibacterial fabric had little change, and even the hydrophilcity improved to certain extent. The antibacterial fabric treated by AgNPs@HTCS was safe and had great application potential.