Asunto(s)
Coartación Aórtica/diagnóstico , Enfermedad de Moyamoya/diagnóstico , Angioplastia Coronaria con Balón , Anticoagulantes/uso terapéutico , Coartación Aórtica/cirugía , Aspirina/uso terapéutico , Bloqueadores de los Canales de Calcio/uso terapéutico , Niño , Angiografía Coronaria , Dexametasona/uso terapéutico , Diagnóstico Diferencial , Quimioterapia Combinada , Femenino , Glucocorticoides/uso terapéutico , Humanos , Angiografía por Resonancia Magnética , Enfermedad de Moyamoya/tratamiento farmacológico , Nimodipina/uso terapéutico , RecurrenciaRESUMEN
The complete D-loop region of mitochondrial DNA from 206 individuals in 16 Chinese indigenous cattle breeds was sequenced and analyzed to detect variability of D-loop region of mitochondria DNA for those breeds. The results showed as follows: 101 variations and 99 haplotypes were found, in which 73 haplotypes were of Bos taurus and the other 26 haplotypes were of Bos indicus, and the average number of nucleotide differences was 22.6920, haplotype diversity was 0.9320, and nucleotide diversity was 0.0227, indicating high genetic diversity in Chinese indigenous cattle breeds. According to the NJ phylogenetic tree, 16 cattle breeds were divided into two clades, Bos taurus and Bos indicus. Based on the Network graphics, the 73 haplotypes of Bos taurus were classified into 3 groups and the 26 haplotypes of Bos indicus were classified into 5 groups. It was inferred that cattle breeds of Bos indicus in China had experienced at least 4 population expansions during their movement. There was only 16% of H3 haplotype sequences similar to the sequence of Nellore, and 84% of those sequences had purine C variation in Chinese indigenous cattle breeds through the analysis on their common H3 haplotypes. It was concluded that those purine C decrease was possibly originated in Chinese Bos indicus.
Asunto(s)
Cruzamiento , Bovinos/genética , ADN Mitocondrial/química , Variación Genética , Origen de Réplica/genética , Animales , Secuencia de Bases , China , ADN Mitocondrial/análisis , Haplotipos , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Polimorfismo Genético , Alineación de SecuenciaRESUMEN
The genetic variations of microsatellites in intron 2 of the porcine adipocyte fatty acid-binding protein (A-FABP) genes were investigated in 420 pigs including Wuzhishan pig, Yimeng black pig, Hanjiang black pig, Laiwu pig, Beijing black pig, Min pig, Chenghua pig, Neijiang pig, Erhualian pig, Bama xiang pig and Large White pig. The results suggested as follows: (i)PIC of the Wuzhishan pig breed is the highest (0.7904) and 11 alleles were detected. Compared with Large White pig,Chinese pig breeds showed a great polymorphism of A-FABP microsatellites except Beijing black pig in which only 2 alleles were detected. (ii) Only Min pig, Bama xiang pig, Beijing black pig and Large White pig were in Hardy-Weinberg equilibrium. (iii) The analysis of genetic differentiation showed that the average value of the A-FABP gene differentiation of 10 Chinese pigs is about 40.83%.
Asunto(s)
Proteínas de Unión a Ácidos Grasos/genética , Repeticiones de Microsatélite , Porcinos/genética , Alelos , Animales , Cruzamiento , China , Frecuencia de los Genes , Heterocigoto , Intrones , Polimorfismo Genético , Porcinos/clasificaciónRESUMEN
Three variant restriction sites of porcine H-FABP gene,including HinfI-RFLP in 5'-upstream, HaeIII-RFLP and HinfI*-RFLP in intron 2, were confirmed by PCR-RFLP method. The polymorphic fragments were cloned and sequenced. The results revealed a single nucleotide substitution of T-->C at position 1324 for HinfI-RFLP,C-->G at position 1811 for HaeIII-RFLP and T-->C at position 1970 for HinfI*-RFLP, respectively.
