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1.
Immun Inflamm Dis ; 9(4): 1428-1438, 2021 12.
Artículo en Inglés | MEDLINE | ID: mdl-34647429

RESUMEN

INTRODUCTION: Diabetic foot ulcer infection (DFI) is an infectious disease of the skin and soft tissue in diabetics notorious for making rapid progress and being hard to cure. Staphylococcus aureus (S. aureus), most frequently detected in DFI, recently was suggested as an intracellular pathogen that can invade and survive within mammalian host cells. Autophagy in macrophages plays a vital immune role in combating intracellular pathogens through bacterial destruction, but there is a lack of empirical research about the infection characteristics and autophagy in diabetic skin infection. METHODS: Here, we used streptozotocin-induced Sprague Dawley rats as a diabetic skin wound model to examine the S. aureus clearance ability and wound healing in vitro. Western blot and immunofluorescence staining were used to evaluate the autophagic flux of the macrophages in diabetic rats dermis, even with S. aureus infection. RESULTS: We demonstrated that infections in diabetic rats appeared more severe and more invasive with weakened pathogen clearance ability of the host immune system, which coincided with the suppressed autophagic flux in dermal macrophages, featured by a significant increase in endogenous LC3II/I and in p62. CONCLUSIONS: Our results first provided convincing evidence that autophagy of macrophages was dysfunctional in diabetes, especially after being infected by S. aureus, which weakens the intracellular killing of S. aureus, potentially worsens the infections, and accelerates the infection spread in the diabetic rat model. Further understanding of the special immune crosstalk between diabetes host and S. aureus infection through autophagic factors will help to explain the complex clinical phenomenon and guarantee the development of effective therapies for diabetic foot infections.


Asunto(s)
Diabetes Mellitus Experimental , Infecciones Estafilocócicas , Animales , Autofagia , Macrófagos , Ratas , Ratas Sprague-Dawley , Staphylococcus aureus , Estreptozocina/toxicidad
2.
J Cancer ; 11(21): 6445-6453, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33033528

RESUMEN

The main diagnostic indicators of ovarian cancer (OC), including carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4), show good sensitivity and poor specificity or vice versa. This study investigated changes in CA125 and HE4 expression and their correlation in serum-derived exosomes of 55 patients with OC (OC group), 33 patients with malignant tumors (non-OC group), and 55 normal controls (NC group). We compared serum and exosomal CA125 and HE4 levels to determine whether their contents in exosomes were elevated. We also compared the diagnostic efficacy of serum HE4, serum CA125, exosomal CA125, and serum HE4+exosomal CA125 in OC using the receiver operating characteristic (ROC) curve. CA125 levels in serum-derived exosomes in all groups significantly increased (P < 0.0001) compared with serum CA125 levels. HE4 was undetected in exosomes. The ROC curve showed the following values: serum CA125: 0.9093 (area), 87.27% (sensitivity), and 90.91% (specificity); serum HE4: 0.9302, 83.64%, and 94.55%; exosomal CA125: 0.9755, 94.55%, and 92.73%; and serum HE4+exosomal CA125: 0.9861, 96.36%, and 92.73%. In conclusion, CA125 can be detected at higher levels in exosomes than in serum, significantly improving OC diagnosis sensitivity. The serum HE4+exosomal CA125 combination significantly improves OC diagnostic efficiency.

3.
Clin Biochem ; 49(1-2): 32-40, 2016 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-26285075

RESUMEN

OBJECTIVES: The purpose of this study was to evaluate the performance of human epididymis protein 4 (HE4) and the Risk of Ovarian Malignancy Algorithm (ROMA) in early stage epithelial ovarian cancer (EOC) detection in patients in southern China. Additionally, this study proposes a possible ideal cut-off value for each marker to its own population in South China. DESIGN AND METHODS: Serum HE4 and CA125 were measured in 756 patients with pelvic masses (275 malignancies, 53 borderline tumors and 428 benign diseases), and their ROMA values were calculated. Areas under the receiver operator characteristic (ROC) curves (AUC) were assessed for HE4, CA125, ROMA and combinations of these biomarkers. RESULTS: Both HE4 and ROMA performed better diagnostically than CA125 alone for early stage EOC, with AUCs ranging from 0.714 for HE4, 0.699 for ROMA, and 0.463 for CA125 in premenopausal subjects, and 0.902 for ROMA, 0.880 for HE4, and 0.256 for CA125 in postmenopausal subjects. CONCLUSIONS: HE4 and ROMA alone were found to be better than CA125 for detecting borderline tumors and early-stage EOC. The optimal cut-off values (HE4: 70pmol/l for all; CA125: 60U/ml for pre- and 35U/ml for postmenopausal women) could notably improve diagnostic performance in EOC detection in patients in southern China.


Asunto(s)
Algoritmos , Antígeno Ca-125/sangre , Neoplasias Glandulares y Epiteliales/diagnóstico , Neoplasias Ováricas/diagnóstico , Proteínas/análisis , Adulto , Carcinoma Epitelial de Ovario , Estudios de Casos y Controles , China , Diagnóstico Precoz , Femenino , Humanos , Persona de Mediana Edad , Sensibilidad y Especificidad , Proteína 2 de Dominio del Núcleo de Cuatro Disulfuros WAP
4.
BMC Infect Dis ; 15: 312, 2015 Aug 05.
Artículo en Inglés | MEDLINE | ID: mdl-26243258

RESUMEN

BACKGROUND: Streptococcus pneumoniae infections are a major cause of global morbidity and mortality, and the emergence of antibiotic-resistant Streptococcus pneumoniae strains has been increasingly reported. This study provides up-to-date information on bacterial serotype distribution and drug resistance from S. pneumoniae clinical isolates that could guide prevention and treatment strategies for pneumococcal disease in China. METHODS: A total of 94 S. pneumoniae isolates were collected from outpatients and inpatients at one Chinese hospital from 2011-2013. Drug susceptibility and resistance was determined by minimum inhibitory concentrations (MICs). Capsular serotypes were identified by the quellung reaction test and multiplex polymerase chain reaction. RESULTS: Fifteen serotypes were identified among the 94 S. pneumoniae clinical isolates that were collected. Prevalent serotypes were 19F (42.6 %), 19A (8.5 %), 3 (8.5 %), and 6B (7.4 %). Potential immunization coverage rates for the 7-, 10- and 13-valent pneumococcal polysaccharide conjugate vaccines were 59.6, 62.6, and 79.6 %, respectively. Resistance rates to tetracycline, erythromycin, and trimethoprim/sulfamethoxazole were 91.2, 80.2 and 63.8 %, respectively. Resistance rates to penicillin, amoxicillin, ceftriaxone, and cefotaxime were 47.3, 34.1, 19.8, and 18.7 %, respectively. In almost all cases, antimicrobial resistance of the S. pneumoniae isolates in patients five years or younger was higher than isolates collected from patients aged 51 years or older. CONCLUSION: Prevalent serotypes among the 94 S. pneumoniae clinical isolates were 19F, 19A, 3, and 6B. The 13-valent pneumococcal polysaccharide conjugate vaccine covered the majority of the serotypes identified in this sample. Drug resistance varied among different serotypes and age groups. Clinical precautions should be taken to avoid the development of multidrug resistance in this potential human pathogen.


Asunto(s)
Infecciones Neumocócicas/diagnóstico , Streptococcus pneumoniae/genética , Adolescente , Adulto , Anciano , Antibacterianos , Niño , China/epidemiología , ADN Bacteriano/análisis , ADN Bacteriano/metabolismo , Farmacorresistencia Bacteriana Múltiple/efectos de los fármacos , Femenino , Hospitales , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa Multiplex , Infecciones Neumocócicas/epidemiología , Infecciones Neumocócicas/microbiología , Vacunas Neumococicas/inmunología , Prevalencia , Serotipificación , Streptococcus pneumoniae/inmunología , Streptococcus pneumoniae/aislamiento & purificación , Vacunas Conjugadas/inmunología , Adulto Joven
5.
Zhonghua Liu Xing Bing Xue Za Zhi ; 32(9): 924-6, 2011 Sep.
Artículo en Chino | MEDLINE | ID: mdl-22340884

RESUMEN

OBJECTIVE: To evaluate the application of the multiple tumor markers's protein chip (C12 chip) in the screen program of the elderly. METHODS: The C12 chip included alpha-fetoprotein (AFP), neuron-specific enolase (NSE), prostate special antigen (PSA), free-PSA (f-PSA), carcinoembryonic antigen (CEA), carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153), carbohydrate antigen 199 (CA199), carbohydrate antigen 242 (CA242), human chorionic gonadotropin-beta (ß-HCG), human growth hormone (HGH)and ferritin. The sera of the 399 healthy elderly under the screening program and 1791 adults were detected by the C12 chip. Definition of positive was set as: one or more tumor markers higher than or equal to the reference value. RESULTS: The positive rates of AFP, PSA, f-PSA, CEA, CA125, CA153 and CA199 between the elderly group and the adult group were significantly different (P < 0.05). The normal test value of AFP, PSA, f-PSA, CEA, CA125, CA199, CA242 and ferritin between the elderly group and the adult group were significantly different (P < 0.05). CONCLUSION: AFP, PSA, f-PSA, CEA, CA125, CA153 and CA199 of the C12 chip were useful in the screening program of the elderly to discover the sign of tumor at an early stage.


Asunto(s)
Antígenos de Neoplasias/sangre , Biomarcadores de Tumor/sangre , Tamizaje Multifásico/métodos , Análisis por Matrices de Proteínas , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Antígenos de Carbohidratos Asociados a Tumores/sangre , Antígeno Ca-125/sangre , Antígeno CA-19-9/sangre , Antígeno Carcinoembrionario/sangre , Humanos , Masculino , Persona de Mediana Edad , Fosfopiruvato Hidratasa/sangre , Adulto Joven , alfa-Fetoproteínas
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