Application of a Spiral Symmetric Stream Anaerobic Bioreactor for treating saline heparin sodium pharmaceutical wastewater: Reactor operating characteristics, organics degradation pathway and salt tolerance mechanism.

In this study, a Spiral Symmetry Stream Anaerobic Bioreactor (SSSAB) was adopted for treating actual saline heparin sodium pharmaceutical wastewater (HSPW). After adaptation, under the influent COD of 8731 mg/L, OLR of 6.98 kg COD/(m³â€¢d) and salinity of 3.57 wt%, the COD removal reached up to 82%. This value is much higher than the reported for the other reactors at similar salinity. Benzenes are the major organic compounds in HSPW. The main rate-limiting steps are the degradations of phenol and p-cresol. In addition, the degradation pathways of typical benzenes in HSPW were analyzed. After adaptation, the soluble salt content in the granular sludge increased, and the bacterial extracellular polymers (EPS), especially tightly-bound EPS also significantly increased. 16S rRNA analysis revealed that the microbial community in the anaerobic granular sludge (AGS) had become adapted to the HSPW treatment since Mesotoga (12.4%), Anaerophaga (9.0%), Oceanotoga (6.1%) and Aminobacterium (4.1%) increased from previously below 1.0% values. The relative abundance of Methanosarcina in the upper layer of the reactor (68.7%) is significantly higher than that at the bottom (3.8%). This proves the superiority of the SSSAB structure. Finally, a model for salt-tolerant microorganisms is given, which proposes a mechanism for this study and provides reference for other anaerobic biological treatments of high-salt containing wastewater.

Anaerobic co-digestion of textile dyeing sludge: Digestion efficiency and heavy metal stability.

Anaerobic co-digestion (AcoD) has become an important mean for the stabilization and recycling of textile dyeing sludge (TDS). Using the soybean okara byproduct (SOB) as a co-digestion substrate, the effects on AcoD performance and heavy metal stability were studied. The results indicated that the optimal mixing ratio was 1:1 (calculated by total sloid). Under this condition, the SCOD removal efficiency was 64% (that of TDS alone and SOB alone were 47% and 48%, respectively) and the cumulative methane production field was 503 L CH4/kg VS (that of TDS alone and SOB alone were 435 L CH4/kg VS and 408 L CH4/kg VS, respectively). At the same time, the addition of SOB could also enhance the stability of heavy metals (Zn, Cu, Cr and Ni) in TDS. Remarkably, that could increase the steady state content nickel from 47.98% to 57.21%, while anaerobic digestion of TDS caused no increase but a decrease (only 42.13%). According to the risk assessment code analyses, the AcoD of TDS by SOB can significantly reduce the ecotoxicity risk caused by Ni, Zn and Cr.

Treatment of polyvinyl alcohol containing wastewater in two stage spiral symmetrical stream anaerobic bioreactors coupled a sequencing batch reactor.

This work aimed to study the treatment of polyvinyl alcohol containing wastewater (PVA-containing wastewater) discharged from textile industry. The batch experiment verified the feasibility of anaerobic treatment and determined that the optimal substrate COD was around 3000 mg/L. The single spiral symmetrical stream anaerobic bioreactor (SSSAB) was used for treating PVA-containing wastewater, which shows the stability of SSSAB and the improvement of biodegradability of wastewater. Finally, two stage SSSABs coupled SBR was proposed. By this scheme, under the influent COD of 3014 mg/L and PVA of 413 mg/L, the COD and PVA removal reached 89.4% and 90.7%, respectively, which were higher than the values obtained by other schemes. Contribution rates of reactors show that each reactor plays an essential role, and SEM images show the unique of microbial flora in each SSSAB. The SSSAB-SSSAB-SBR process can provide an alternative to the chemical methods for treating PVA-containing wastewater.

Mendelian randomization integrating GWAS and eQTL data revealed genes pleiotropically associated with major depressive disorder.

Previous genome-wide association studies (GWAS) have identified potential genetic variants associated with the risk of major depressive disorder (MDD), but the underlying biological interpretation remains largely unknown. We aimed to prioritize genes that were pleiotropically or potentially causally associated with MDD. We applied the summary data-based Mendelian randomization (SMR) method integrating GWAS and gene expression quantitative trait loci (eQTL) data in 13 brain regions to identify genes that were pleiotropically associated with MDD. In addition, we repeated the analysis by using the meta-analyzed version of the eQTL summary data in the brain (brain-eMeta). We identified multiple significant genes across different brain regions that may be involved in the pathogenesis of MDD. The prime-specific gene BTN3A2 (corresponding probe: ENSG00000186470.9) was the top hit showing pleiotropic association with MDD in 9 of the 13 brain regions and in brain-eMeta, after correction for multiple testing. Many of the identified genes are located in the human major histocompatibility complex (MHC) region on chromosome 6 and are mainly involved in the immune response. Our SMR analysis indicated that multiple genes showed pleiotropic association with MDD across the brain regions. These findings provided important leads to a better understanding of the mechanism of MDD and revealed potential therapeutic targets for the prevention and effective treatment of MDD.

Anaerobic biodegradation of soybean-process wastewater: Operation strategy and sludge bed characteristics of a high-performance Spiral Symmetric Stream Anaerobic Bioreactor.

A 300m3/d demonstration project of soybean-process wastewater has been established recently with a Spiral Symmetric Stream Anaerobic Bioreactor (SSSAB) as the core. In order to obtain the optimal operation strategy for a full-scale SSSAB and to make it run efficiently and stably in a demonstration project, a Pilot-scale SSSAB (P-SSSAB, effective volume 100 L) was performed for the treatment of soybean-process wastewater over 216 days. The volumetric load rate (VLR) range of the P-SSSAB was 0.32~27.17 kg COD/(m3·d), where the highest VLR [27.17 kg COD/(m3·d)] was 2.01 times to the highest value [13.5 kg COD/(m3·d)] reported. The pH and VFA/ALK of the effluent from the P-SSSAB were in the range of 6.9 up to 9.2 and 0.03 up to 0.17, respectively. The methane yield of the P-SSSAB increased from 0.03 m3/kg COD to 0.47 m3/kg COD, which was 3.36 times to the maximum value (0.14 m3/kg COD) reported. To meet the influent requirement of the aerobic biological treatment in demonstration project (influent COD ≤ 1.5 g/L), the maximum VLR of SSSAB was optimal at about 22 kg COD/(m3·d). By analyzing the sludge bed characteristics of the P-SSSAB, it was obvious that zone I (the bottom of the bed) was the major contributor of the COD removal, while zone III (the upper part of the bed) was the major contributor for the NH4+-N increase. The anaerobic granular sludge (AGS) in the bed showed a good granulation. The average MLVSS/MLSS value in sludge bed was about 0.7, and PN/PS in TB-EPS (zone I, II and III) increased to 6.830, 4.257, and 3.747, respectively. SMA and coenzyme F420 values of zone III were the maximum [666.35 ml CH4/(g VSS·d) and 0.690 mol/g VSS, respectively]. According to the analysis obtained from the 16S rRNA high-throughput sequencing, the microbial community in the AGS had been more specific to the soybean-process wastewater since the bacteria Firmicutes were increased. The relative abundance of microbe which perform direct interspecies electron transfer (DIET) for the syntrophic degradation of VFAs and production of the methane has been increased significantly, such as the bacteria Syntrophomonas and archaea Methanosaeta.

Simulating the synergy of electron donors and different redox mediators on the anaerobic decolorization of azo dyes: Can AQDS-chitosan globules replace the traditional redox mediators?

During anaerobic treatment of azo dye wastewater, the decolorization efficiency is low and dissolved redox mediators (RMs) added to the system are easy lost. In order to solve these issues, immobilized RMs have been a hot area of research. In this study a novel immobilized RM material, disodium anthraquinone-2,6-disulfonate (AQDS)-chitosan globules, which is natural, highly efficient and environmentally friendly, was prepared. Compared with natural immobilized RMs (activated carbon) and dissolved RMs (AQDS), it can be considered that it has a significant strengthening effect on the anaerobic biological degradation and decolorization of azo dye wastewater. An electron donor (ED, glucose) or RM (AQDS solution) was dosed into an anaerobic reactor to determine the enhancing effect and appropriate concentration for the decolorization treatment. The results indicate that a certain concentration of ED or RM [300 mg/L (1.667 mmol/L) glucose or 200 µmol/L AQDS solution] can improve effectively the anaerobic biological degradation and decolorization effect of azo dye wastewater. While by adding both 300 mg/L (1.667 mmol/L) glucose and 300 µmol/L AQDS (the concentrations were the initial reactive concentrations) together the decolorization efficiency was improved further. At the same time, the synergy of ED (glucose) and RM (AQDS solution) on the anaerobic decolorization of azo dye was simulated by the central combination design. A mathematical model for the decolorization efficiency has been established. According to this model, the hydraulic retention time of the best decolorization speed and efficiency has been obtained.