A comparison of the effects of two protocols of concurrent resistance and aerobic training on physical fitness in middle school students.

Objective: This study aimed to compare the effects of two concurrent training (CT) protocols on the physical fitness of middle school students. Method: A 12-week quasi-experimental pre-test/post-test study was conducted with 157 middle school students (age = 12.48 ± 0.34, n = 90 females) divided into three groups: CT group A (CT-0h) received combined resistance training (RT) and aerobic training (AT) in each physical education session, CT group B (CT-48h) received RT and AT across two separate physical education classes 48 h apart, and a control group (Con) received no training. Training occurred twice a week. Test indicators included cardiorespiratory fitness (CRF) measured by estimated VO2max and 20 m shuttle run (laps), as well as muscle strength assessed through long jump, vertical jump, and handgrip strength. Results: The intervention groups exhibited significant increases in estimated VO2max and muscle strength compared to their baseline values (p < 0.05). Both CT-0h and CT-48h groups demonstrated significant improvements in 20 m shuttle run (laps) (mean difference: 8.88 laps, p < 0.01; mean difference: 4.81 laps, p < 0.01, respectively), standing long jump (mean difference: 6.20 cm, p < 0.01; mean difference: 3.68 cm, p < 0.01, respectively), vertical jump (mean difference: 4.95 cm, p < 0.01; mean difference: 4.04 cm, p < 0.01, respectively), and handgrip strength (mean difference: 11.17 kg, p < 0.01; mean difference: 6.99 kg, p < 0.01, respectively). CT-0h group exhibited significantly increased estimated VO2max (mean difference: 1.47 ml/kg/min, p < 0.01) compared to the CT-48h group. Conclusion: Both CT programs effectively improved adolescents' physical fitness indicators. However, the program that integrated RT and AT within the same physical education class demonstrated superior enhancement in adolescents' CRF.

Association of physical activity with socio-economic status and chronic disease in older adults in China: cross-sectional findings from the survey of CLASS 2020 after the outbreak of COVID-19.

BACKGROUND: In 2021, China had a population of 264·01 million individuals over the age of 60, indicating a high prevalence of chronic diseases. Among older adults, physical inactivity (PI) is a significant risk factor for chronic diseases. However, few studies have been conducted on the correlation of physical activity (PA) with the economic status, geography and chronic disease risks in Chinese elderly. The objectives of this study were to better understand the distribution of PA among older adults in China and its relationship with economic status, geography, and chronic disease risks. METHODS: This study utilized data from the China Longitudinal Aging Social Survey (CLASS) in 2020, post-COVID-19. The study employed a stratified, multistage, probabilistic sampling approach and included 11,396 adults over the age of 59 from 28 provinces in China. Data on demographics, the duration and intensity of PA, history of diseases and personalized factors influencing PA were collected via structured interviews by researchers. In this study, we conducted a comprehensive analysis, employing a range of statistical methods including descriptive analysis, Wilcoxon rank-sum tests, Bayesian networks, and chi-square tests. RESULTS: The prevalence of PI among older adults over 59 in China is 28·82%. Significant regional differences were observed in the duration of PA at different intensities. Older adults residing in more economically developed areas were more likely to engage in moderate-to-vigorous physical activity (MVPA) and exhibited longer sedentary behavior. Economic status and urban-rural disparities consistently emerged as direct influential factors across all intensity types. Chronic disease risks were significantly lower in active older adults compared to inactive ones. Lack of social guidance, family support, and personal inclination towards sedentary behavior were the main personalized factors affecting PA among older adults, and these factors could be relatively easily modified. CONCLUSIONS: Economic status, geography, and living areas (urban and rural) significantly influenced the distribution of physical activities in China. Particularly, economic status and living areas acted as direct factors. Older adults reaching the recommended standards for PA had significantly lower chronic disease risks, highlighting the importance of improving personalized factors which are crucial for promoting PA.

Chromosome-level assembly and gene annotation of Decapterus maruadsi genome using Nanopore and Hi-C technologies.

Decapterus maruadsi is one of the representative offshore fish in the Western Pacific. Since the last century, it has become a commercially valuable marine fishery species in the Western Pacific region. Despite its high economic value, there is still a lack of high-quality reference genome of D. maruadsi in germplasm resource evaluation research. Here we report a chromosome-level reference genome of D. maruadsi based on Nanopore sequencing and Hi-C technologies. The whole genome was assembled through 169 contigs with a total length of 723.69 Mb and a contig N50 length of 24.67 Mb. By chromosome scaffolding, 23 chromosomes with a total length of 713.58 Mb were constructed. In addition, a total of 199.49 Mb repetitive elements, 33,515 protein-coding genes, and 6,431 ncRNAs were annotated in the reference genome. This reference genome of D. maruadsi will provide a solid theoretical basis not only for the subsequent development of genomic resources of D. maruadsi but also for the formulation of policies related to the protection of D. maruadsi.

Bursicon regulates wing expansion via PKA in the oriental fruit fly, Bactrocera dorsalis.

BACKGROUND: Bursicon is a heterodimeric neuropeptide that is involved in many physiological activities such as cuticle tanning, wing expansion, reproduction and immunity in insects. In this study, the role of bursicon in the wing expansion was investigated in Bactrocera dorsalis, an important invasive insect pest in agriculture. RESULTS: The cDNA sequences and deduced amino acids of bursicon genes (named BdBurs-α and BdBurs-ß) were determined, and two proteins typically contained 11 cysteine residues in conserved positions that were highly conserved in other insect species. The spatiotemporal expressions of bursicon genes showed that higher expression occurred at the pupal, early adult stage and ovaries, and lower expression at the late larval stage and in wing tissue (8-day-old pupae). Dysfunction of bursicon genes by dsRNA microinjection into 5-day-old pupae reduced PKA (a downstream component of the bursicon pathway) activity and resulted in malformed adult wings. PKA inhibitor injection into 5-day-old pupae also resulted in similar phenotypes. Hematoxylin & eosin staining of the adult wing showed that RNAi and PKA inhibitor treatment reduced the thickness of the wing cuticle, which wing cuticle thickness were ≈50% thinner than in the control. Furthermore, the expression of hedgehog (Bdhh) (one of 10 tested genes related to wing development) was significantly upregulated after RNAi and PKA inhibitor application. CONCLUSION: The results indicate that bursicon plays a crucial role in the wing expansion of B. dorsalis, suggesting bursicon genes have potential to be the targets for B. dorsalis control. © 2023 Society of Chemical Industry.

Comparison of different prediction models for estimation of walking and running energy expenditure based on a wristwear three-axis accelerometer.

Objective: Objectively and efficiently measuring physical activity is a common issue facing the fields of medicine, public health, education, and sports worldwide. In response to the problem of low accuracy in predicting energy consumption during human motion using accelerometers, a prediction model for asynchronous energy consumption in the human body is established through various algorithms, and the accuracy of the model is evaluated. The optimal energy consumption prediction model is selected to provide theoretical reference for selecting reasonable algorithms to predict energy consumption during human motion. Methods: A total of 100 subjects aged 18-30 years participated in the study. Experimental data for all subjects are randomly divided into the modeling group (n = 70) and validation group (n = 30). Each participant wore a triaxial accelerometer, COSMED Quark pulmonary function tester (Quark PFT), and heart rate band at the same time, and completed the tasks of walking (speed range: 2 km/h, 3 km/h, 4 km/h, 5 km/h, and 6 km/h) and running (speed range: 7 km/h, 8 km/h, and 9 km/h) sequentially. The prediction models were built using accelerometer data as the independent variable and the metabolic equivalents (METs) as the dependent variable. To calculate the prediction accuracy of the models, root mean square error (RMSE) and bias were used, and the consistency of each prediction model was evaluated based on Bland-Altman analysis. Results: The linear equation, logarithmic equation, cubic equation, artificial neural network (ANN) model, and walking-and-running two-stage model were established. According to the validation results, our proposed walking-and-running two-stage model showed the smallest overall EE prediction error (RMSE = 0.76 METs, Bias = 0.02 METs) and the best performance in Bland-Altman analysis. Additionally, it had the lowest error in predicting EE during walking (RMSE = 0.66 METs, Bias = 0.03 METs) and running (RMSE = 0.90 METs, Bias < 0.01 METs) separately, as well as high accuracy in predicting EE at each single speed. Conclusion: The ANN-based walking-and-running two-stage model established by separating walking and running can better estimate the walking and running EE, the improvement of energy consumption prediction accuracy will be conducive to more accurate to monitor the energy consumption of PA.

Effects of poling camber angle on the biomechanics of cross-country sit-skiing.

Cross-country sit-skiers use double poling (DP) technique to drive the slide. The aim of this study is to analyze how poling camber angle affect the capacity of power output and biomechanical parameters of the DP process. Twenty-four non-disabled college students (24.67 ± 1.46 years old) were recruited to perform three successive 30-s maximal effort tests with different poling camber angles of 0°, 15°, 24° and 30° using a sit-skiing ergometer. The biomechanical parameters, output power and muscle activation of the subjects were analyzed. The results showed that DP output power increased with the increase of poling camber angle at 15° (597.78 ± 150.31 J), 24° (610.94 ± 158.96 J, P = 0.011) and 30° (629.10 ± 168.78 J, P < 0.001) compared with 0° (590.65 ± 148.95 J). However, effective output power decreased with the increase of camber angle. Poling with camber angle of 24° had the shortest cycle time 1.53 ± 0.17 s, compared with other abduction angle (0°, 1.57 ± 0.19 s, 15°, 1.55 ± 0.16 s, and 30°, 1.56 ± 0.19 s). Compared with 0° (1.02 ± 0.14 m), the cycle distance significantly increased at poling camber angles of 24° (1.07 ± 0.12 m, P = 0.029) and 30° (1.11 ± 0.13 m, P < 0.001). With the increase of poling camber angle, the shoulder and elbow joint range of motions and joint moments were significantly increased. This study found that poling with shoulder abducted increased the output power but decreased the efficiency. By analyzing the poling angle and poling force, we find that the optimal poling camber angle may depend on the terrain or the skiing speed. These results may guide the competition techniques and tactics in the matches, and may further influence the strength-training programs of cross-country sit-skiing athletes.

The Bidirectional Correlation between Fundamental Motor Skill and Moderate-to-Vigorous Physical Activities: A Systematic Review and Meta-Analysis.

BACKGROUND: Physical activity in early life has positive health effects, but few children meet the physical activity recommendations. Fundamental motor skills (FMS) are related to physical activity and according to the theory, physical activity drives them in the early years and vice versa. However, no study has conducted a meta-analysis of the association between moderate-to-vigorous intensity physical activity (MVPA) and early FMS. This meta-analysis examined the bidirectional correlation between MVPA and domain-specific FMS in preschool children. METHODS: We searched PubMed, Embase, and Cochrane Library databases for articles published up to August 2023. Cross-sectional and longitudinal studies were included if they targeted children (3-6 years old) as the study population. The association between objectively measured MVPA and FMS was evaluated. RESULTS: We found 445 titles and abstracts. A total of ten studies (eleven datasets) and 2514 children met the inclusion criteria, including eight cross-sectional studies and three longitudinal studies. When using MVPA as the exposure, no associations were found with locomotor skills (ß = 0.83, 95% CI: -0.08, 1.74, p = 0.07) and gross motor skills (ß = 2.72, 95% CI: -0.28, 5.72, p = 0.08), but an association with object management skills was found (ß = 0.18, 95% CI: 0.06, 0.30, p = 0.001). When MVPA was used as the outcome, no associations were observed between locomotor skills (ß = 0.06, 95% CI: -0.35, 0.47, p = 0.79), but associations with object management skills (ß = 0.15, 95% CI: 0.02, 0.27, p = 0.02) and gross motor skill were found (ß = 0.56, 95% CI: 0.38, 0.75, p = 0.001). The sensitivity analysis showed that the results must be treated with caution. CONCLUSION: We found that gross motor skill (exposure) was positively associated with MVPA (outcome) in preschoolers. Object management skills were positively associated with MVPA (exposure) and MVPA (outcome) in preschoolers. In contrast, MVPA as an exposure was not associated with locomotor skills and gross motor skills. The results may suggest that promoting FMS is important for preschool children's MVPA.

Population structure and genome-wide evolutionary signatures reveal putative climate-driven habitat change and local adaptation in the large yellow croaker.

The large yellow croaker (Larimichthys crocea) is one of the most economically valuable marine fish in China and is a notable species in ecological studies owing to a serious collapse of wild germplasm in the past few decades. The stock division and species distribution, which have important implications for ecological protection, germplasm recovery, and fishery resource management, have been debated since the 1960s. However, it is still uncertain even how many stocks exist in this species. To address this, we evaluated the fine-scale genetic structure of large yellow croaker populations distributed along the eastern and southern Chinese coastline based on 7.64 million SNP markers. Compared with the widely accepted stock boundaries proposed in the 1960s, our results revealed that a climate-driven habitat change probably occurred between the Naozhou (Nanhai) Stock and the Ming-Yuedong (Mindong) Stock. The boundary between these two stocks might have shifted northwards from the Pearl River Estuary to the northern area of the Taiwan Strait, accompanied by highly asymmetric introgression. In addition, we found divergent landscapes of natural selection between the stocks inhabiting northern and southern areas. The northern population exhibited highly agminated signatures of strong natural selection in genes related to developmental processes, whereas moderate and interspersed selective signatures were detected in many immune-related genes in the southern populations. These findings establish the stock status and genome-wide evolutionary landscapes of large yellow croaker, providing a basis for conservation, fisheries management and further evolutionary biology studies. Supplementary Information: The online version contains supplementary material available at 10.1007/s42995-023-00165-2.

Single-cell sequencing of immune cells after marathon and symptom-limited cardiopulmonary exercise.

Vigorous-intensity leisure-time physical activity, such as marathon, has become increasingly popular, but its effect on immune functions and health is poorly understood. Here, we performed scRNA-seq analysis of peripheral blood mononuclear cells (PBMCs) after a bout of symptom-limited cardiopulmonary exercise (CPX) test or marathon. Time-series single-cell analysis revealed the detailed series of landscapes of immune cells in response to short and long vigorous-intensity activities. Reduction of effective T cells was observed with the cell migration and motility pathways enriched in circulation following marathon. Baseline values of PBMCs abundance were reached around 1 h after CPX and 24 h following marathon, but longer time was required for expression recovery of cytotoxicity genes. The ratio of effector/naive T cells was found to change uniformly among the participants and could serve as a better indicator for exercise intensity than the CD4+/CD8+ T cell ratio. Moreover, we identified time-dependent monocyte state transitions after marathon.

The adaptive evolution of Leuciscus waleckii in Lake Dali Nur and convergent evolution of Cypriniformes fishes inhabiting extremely alkaline environments.

Leuciscus waleckii is widely distributed in Northeast Asia and has high economic value. The population in Lake Dali Nur can adapt to extremely alkaline-saline water with bicarbonate over 50 mmol/L (pH 9.6), thus providing an exceptional model for exploring the mechanisms of adaptive evolution under extreme alkaline environments. Here, we assembled a high-quality chromosome-level reference genome for L. waleckii from Lake Dali Nur. Based on the resequencing of 85 individuals from divergent populations, the historical population size of L.waleckii in Lake Dali Nur dramatically expanded in a thousand years approximately 13,000 years ago, and experienced a cliff recession in the process of adapting to the alkaline environment of Lake Dali Nur approximately 6,000 years ago. Genome scans between freshwater and alkaline populations further revealed the significant selective sweep regions from Lake Dali Nur, which harbour a set of candidate genes involved in hypoxia tolerance, ion transport, acid-base regulation and nitrogen metabolism. 5 alkali population-specific nonsynonymous mutations were identified in CA15 gene copies. In addition, two sites with convergent amino acid mutation were detected in the RHCG-a gene among several alkali environment adapted Cypriniformes fish. Our findings provide comprehensive insight into the genomic mechanisms of L. waleckii and reveal their adaptative evolution under extreme alkaline environments.

Genome-wide evolutionary signatures of climate adaptation in spotted sea bass inhabiting different latitudinal regions.

Consideration of the thermal adaptation of species is essential in both evolutionary biology and climate-change biology because it frequently leads to latitudinal gradients of various phenotypes among populations. The spotted sea bass (Lateolabrax maculatus) has a broad latitudinal distribution range along the marginal seas of the Northwest Pacific and thus provides an excellent teleost model for population genetic and climate adaptation studies. We generated over 8.57 million SNP loci using whole-genome resequencing from 100 samples collected at 14 geographic sites (five or ten samples per site). We estimated the genetic structure of the sampled fish and clustered them into three highly differentiated populations. The genetic differentiation pattern estimated by multivariable models combining geographic distance and sea surface temperature differences suggests that isolation by distance and isolation by environment both have significant effects on this species. Further investigation of genome-wide evolutionary signatures of climate adaptation identified many genes related to growth, muscle contraction, and vision that are under positive natural selection. Moreover, the contrasting patterns of natural selection in high-latitude and low-latitude populations prompted different strategies of trade-offs between growth rate and other traits that may play an essential role in adaptation to different local climates. Our results offer an opportunity to better understand the genetic basis of the phenotypic variation in eurythermal fishes inhabiting different climatic regions.

Effects of running-based versus body-weight-based high-intensity interval training on physical fitness in healthy adolescents.

Objectives: High-intensity interval training improves aerobic endurance, but the effectiveness of different training protocols is unclear. This study compared the effects of running-based high-intensity interval training (R-HIIT) and body weight-based high-intensity interval training (B-HIIT) on physical fitness in adolescents. Methods: This was a pre-and post-test quasi-experimental design in which a seventh-grade natural class was randomly selected from three homogeneous middle schools, and then the three natural classes were randomly divided into three groups: the R-HIIT group (n = 54), the B-HIIT group (n = 55), and the control group (Con, n = 57). Both intervention groups exercised twice a week for 12 weeks with a 2:1 (1 min:30 s) load-interval ratio and exercise intensity controlled at 70%-85% maximum heart rate. R-HIIT was in the form of running, and B-HIIT was in the form of resistance exercises using the participants' body weight. The control group was instructed to continue their normal behavior. cardiorespiratory fitness, muscle strength and endurance, and speed were measured before and after the intervention. Statistical differences between and within groups were determined using repeated measures analysis of variance. Results: Compared to the baseline, both the R-HIIT and B-HIIT intervention groups significantly improved CRF, muscle strength, and speed (p < 0.05). The B-HIIT group was significantly better than R-HIIT in improving CRF (4.48 mL/kg/min vs 3.34 mL/kg/min, p < 0.05), and only the B-HIIT improved sit-up muscle endurance (ηp 2 = 0.30, p < 0.05). Conclusion: The B-HIIT protocol was significantly more effective than the R-HIIT protocol in developing CRF and improving muscle health indicators.

Acute arterial stiffness responses to on-ball balance exercises in young and middle-aged adults: Role of posture and cumulative effects.

Objective: To examine the acute arterial stiffness changes after maintaining one bout of balance on Swiss ball using different postures in young and middle-aged adults, and to evaluate the cumulative exposure effects on arterial stiffness after multiple exercise bouts in middle-aged adults. Methods: Using crossover design, we first enrolled 22 young adults (24.0 ± 1.1 years) and randomized them to non-exercise control (CON), on-ball balance exercise trial lasting 1 × 5 min in kneeling posture (K1) and sitting posture (S1). In a following crossover experiment, 19 middle-aged adults (53.0 ± 4.7 years) were randomized to non-exercise control (CON), on-ball balance exercise trial lasting 1 × 5 min in kneeling posture (K1) and in sitting posture (S1), and on-ball balance exercise trial lasting 2 × 5 min in kneeling posture (K2) and in sitting posture (S2). Cardio-ankle vascular index (CAVI), an indicator of systemic arterial stiffness, was measured at baseline (BL), immediately after (0 min), and every 10 min after exercise. CAVI changes from BL in the same trial (⊿CAVI) were used for analysis. Results: In K1 trial, ⊿CAVI decreased significantly at 0 min (p < 0.05) in both young and middle-aged adults; however in S1 trial, ⊿CAVI at 0 min increased significantly in young adults (p < 0.05), with ⊿CAVI tending to increase in middle-aged adults. Bonferroni post-test revealed that at 0 min, ⊿CAVI of K1 in both young and middle-aged adults, and ⊿CAVI of S1 in young adults differed significantly from that of CON (p < 0.05). In middle-aged adults, ⊿CAVI decreased significantly at 10 min compared to BL in K2 trial (p < 0.05), and increased at 0 min compared to BL in S2 trial (p < 0.05); however, difference compared to CON was not significant. Conclusion: Single on-ball balance bout in kneeling posture improved arterial stiffness transiently in both young and middle-aged adults; however, sitting posture elicited opposite changes, and this happened only in young adults. Multiple balance bouts resulted in no significant change in arterial stiffness in middle-aged adults.

Association between Air Pollution and Physical Activity and Sedentary Behavior among Adults Aged 60 Years or Older in China: A Cross-Sectional Study.

BACKGROUND: Exposure to air pollution is associated with an increased risk of all-cause mortality in older adults. Promoting physical activity (PA) and avoiding sedentary behavior (SB) serve as key strategies to maintain and improve human health. However, ambient air pollution can adversely affect PA and SB, increasing the risks of health problems. This study aimed to visualize national spatial patterns of average AQI concentration, PA, and SB distributions and to examine the associations between air pollution and PA and SB in a national sample of Chinese older adults aged 60 years or older. METHODS: We analyzed the data of the China Longitudinal Aging Social Survey 2020 (CLASS 2020), which sampled 11,399 older men and women from 30 cities in China. Moderate, vigorous, and light PA and SB were measured using the Chinese version of the International Physical Activity Questionnaire-Short Form (IPAQ-C). The environmental measures included the average hourly air quality index (AQI), PM2.5, PM10, and NO2 (µg/m3). The data were analyzed using multivariable linear regression. RESULTS: Increases in the standard deviations (±SD) of AQI, PM2.5, PM10, and NO2 concentrations were associated with decreases in MVPA per week of -2.34 (95%CI = -3.36, -1.32), -2.58 (95%CI = -3.61, -1.55), -1.96 (95%CI = -3.05, -0.08), and -1.19 (95%CI = -2.06, -0.31) and decreases in LPA per week of -6.06 (95%CI = -7.15, -4.97), -4.86 (95%CI = -5.88, -3.85), -4.78 (95%CI = -5.89, -3.68), and -4.59 (95%CI = -5.57, -3.61) h/week, respectively. Increases in one SD of AQI, PM2.5, PM10, and NO2 were associated with increases in SB per week of 1.32 (95%CI = 0.77, 1.88), 0.62 (95%CI = 0.09, 1.14), 1.03 (95%CI = 0.48, 1.59), and 0.98 (95%CI = 0.46, 1.49) h/week, respectively. CONCLUSIONS: The spatial distributions of the average AQI concentration, MVPA, LPA, and SB are useful and allow environmental and health policymakers to identify the areas with the highest priority air pollution environmental equality concerns. AQI was positively associated with MVPA and LPA, and it was negatively associated with SB among older adults. AQI, PM2.5, PM10, and NO2 were hardly associated with women's average time spent engaged in MVPA. Region-specific and multi-level health policy options are needed to reduce ambient air pollution by taking different types of pollutants into account in order to avoid changes in PA and SB in this population, especially in locations with high air pollution concentrations.

Chromosome-level haplotype-resolved genome assembly for Takifugu ocellatus using PacBio and Hi-C technologies.

Takifugu species serve as a model system for evolutionary studies due to their compact genomes and diverse phenotypes. The ocellated puffer (Takifugu ocellatus), characterized by special colouration, is a scarce anadromous species in the genus Takifugu. As an ornamental and tasty fish species, T. ocellatus has moderate economic value. However, the available genomic resources for this pufferfish are still limited. Here, a chromosome-level reference genome, as well as two haploid genomes, was constructed by PacBio HiFi long sequencing and Hi-C technologies. The total length of the reference genome was 375.62 Mb with a contig N50 of 11.55 Mb. The assembled sequences were anchored to 22 chromosomes with an integration efficiency of 93.78%. Furthermore, 28,808 protein-coding genes were predicted. The haplotype-resolved reference genome of T. ocellatus provides a crucial resource for investigating the explosive speciation of the Takifugu genus, such as elucidating evolutionary histories, determining the genetic basis of trait evolution, and supporting future conservation efforts.

Bilateral upper extremity trunk model for cross-country sit-skiing double poling propulsion: model development and validation.

The subacromial impingement syndrome is a high-incidence injury for cross-country sit-skiing skier, which is often accompanied by muscle imbalance. However, at present, no musculoskeletal model has been identified for this sport. Thus, this research aimed to establish a bilateral upper extremity trunk (BUET) musculoskeletal model suitable for cross-country sit-skiing based on OpenSim software and verify the function of the model. By splicing three existing OpenSim models, an upper limb model with 17 segments, 35 degrees of freedom, and 472 musculotendon actuators was established. The clavicle and scapula were modeled as individual bodies and then connected to the torso through a three-degrees-of-freedom rotational joint and to the clavicle through a weld joint, respectively. The five lumbar vertebrae were established separately and coupled into a three-degree-of-freedom joint. Kinematics, kinetic, and EMG signal data of five 15-s maximal effort interval tests were obtained by using seven cameras, ergometers, and surface EMG synchronous collection. Based on the resulting rotator cuff muscle geometry of the model, simulated muscle activation patterns were comparable to experimental data, and muscle-driven ability was proven. The model will be available online ( https://simtk.org/projects/bit ) for researchers to study the muscle activation of shoulder joint movement.

Pif1 family helicases promote mutation avoidance during DNA replication.

Pif1 family 5' → 3' DNA helicases are important for replication fork progression and genome stability. The budding yeast Saccharomyces cerevisiae encodes two Pif1 family helicases, Rrm3 and Pif1, both of which are multi-functional. Here we describe novel functions for Rrm3 in promoting mutation avoidance during DNA replication. We show that loss of RRM3 results in elevated spontaneous mutations made by DNA polymerases Pols ϵ and δ, which are subject to DNA mismatch repair. The absence of RRM3 also causes higher mutagenesis by the fourth B-family DNA polymerase Pol ζ. By genome-wide analysis, we show that the mutational consequences due to loss of RRM3 vary depending on the genomic locus. Rrm3 promotes the accuracy of DNA replication by Pols ϵ and δ across the genome, and it is particularly important for preventing Pol ζ-dependent mutagenesis at tRNA genes. In addition, mutation avoidance by Rrm3 depends on its helicase activity, and Pif1 serves as a backup for Rrm3 in suppressing mutagenesis. We present evidence that the sole human Pif1 family helicase in human cells likely also promotes replication fidelity, suggesting that a role for Pif1 family helicases in mutation avoidance may be evolutionarily conserved, a possible underlying mechanism for its potential tumor-suppressor function.

Evaluating the Effects of Cu2+ on the Development and Reproduction of Spodoptera litura (Lepidoptera: Noctuidae) Based on the Age-Stage, Two-Sex Life Table.

Copper (Cu2+) is a micronutrient that promotes the development and reproduction of organisms. However, with the rapid expansion of modern industry and agriculture, Cu2+ concentrations are increasing, which might have negative impacts on biological and ecological safety. Spodoptera litura is not only an intermittent outbreak pest but also can be used as a model organism to assess environmental and ecological risks. Therefore, the effects of the life history and population parameters of S. litura fed on artificial diets with different Cu2+ concentrations were analyzed using the age-stage, two-sex life table. Our results showed that not only the preadult survival rate but also the intrinsic rate of increase (r) and the finite rate of increase (λ) were significantly increased under exposure to low Cu2+ concentrations (2, 4, and 8 mg/kg). In addition, the population growth of S. litura was significantly faster, indicating that S. litura can adapt well to low concentrations and is likely to undergo outbreaks of damage. Whereas, in addition to a significant reduction in preadult survival rate, population growth rate, pupal weight, pupal length, adult body weight, and oviposition were also significantly reduced under exposure to high Cu2+ concentration (32 mg/kg). And when the concentration reached 64 mg/kg, the survival rate of adults was extremely low, suggesting a decrease in the adaptation of S. litura. These results can help to understand the population dynamics of S. litura and predict potential ecological risks.

Acute effects of one-leg standing on arterial stiffness in older women: Role of the vision condition and standing dose.

Objective: One-leg standing has been used exclusively for static balance testing and training purposes. We investigated the acute effects of one-leg standing with open or closed eyes on arterial stiffness in older women and explored the role of standing dose in arterial stiffness regulation. Methods: Eighteen older women (60 ± 2 years) underwent non-intervention control (CON), one-leg standing with open eyes for 2 × 3 min (SO2), and one-leg standing with closed eyes for 1 × 3 min (SC1), 2 × 3 min (SC2), and 3 × 3 min trials (SC3) in a randomized self-controlled crossover fashion. Arterial stiffness in the cardio-ankle vascular index (CAVI) was measured at baseline (BL), immediately (0 min), and 10 and 20 min after standing. CAVI changes from BL in the same trial (⊿CAVI) were used for analysis. Results: ⊿CAVI of the non-standing and standing side did not change with time in CON and SO2 trials. In SC1, SC2, and SC3 trials, ⊿CAVI of the standing side decreased significantly at 0 min compared to their corresponding BL (p < 0.01) and reverted gradually to the BL level afterward, with ⊿CAVI of the non-standing side undergoing no changes. At the time point of 0 min, only in the SC2 trial, ⊿CAVI of the standing side was significantly lower than that of CON (p < 0.01). Conclusion: One-leg standing with closed eyes, but not with open eyes, resulted in transient arterial stiffness improvement in older women. The improvement was restricted to standing leg, and the moderate standing dose had maximal benefit on arterial stiffness.

An Intervention Pattern of Family Parent-Child Physical Activity Based on a Smartphone App for Preschool Children during COVID-19.

Objective: Children's lifestyles, behaviors, and educational activities were affected by COVID-19. The preschool children struggled with the challenge of learning at home and avoiding a rapid decrease in physical activity (PA). This study tested the effectiveness of a family-based intervention that integrated the family and preschool based on a smartphone app to improve the moderate-to-vigorous PA (MVPA) and physical fitness of preschool children during COVID-19. Methods: This 8-week study was conducted using a quasiexperimental pre- and posttest design with a comparison group. A total of 66 pairs of preschool children (30 boys) and their parents and 44 preschool children (24 boys) and their parents composed the experimental group (EG) and the control group (CG), respectively. PA was measured using a GENEActiv waveform triaxial accelerometer. Children's physical fitness was assessed using a battery test from the Chinese National Measurement Standards on People's Physical Fitness for preschool children. Results: Preschool children and their parents in the EG participated in less sedentary (p < 0.01) and more light PA (p < 0.01) and MVPA (p < 0.01) compared with those in the CG at the late PA assessment. The EG significantly improved the mean performance of tennis ball throw (p < 0.05), balance beam walk (p < 0.01), and continuous jumping on both feet (p < 0.01) compared to the CG. Conclusions: The family parent-child PA intervention based on a smartphone app can effectively increase the MVPA of preschool children and their parents, reduce sedentary time, and improve preschool children's physical fitness. Overall, the family parent-child PA intervention model based on a smartphone app for preschool children designed in this study is feasible and effective.