RESUMEN
Japanese encephalitis virus (JEV) is a viral zoonosis that can cause viral encephalitis, death and disability whose primary vector is the Culex mosquito. Viral infection induces a series of antimicrobial peptide responses in mosquitoes, and the effector defensin enhances JEV replication in mosquitoes. However, the underlying mechanisms by which defensin enhances JEV are not fully understood. Here, we found that mosquito defensin could downregulate the antiviral protein HSC70B and enhance virus infection in mosquitoes. The cell-surface protein HSC70B was significantly downregulated by JEV infection and defensin treatment. Low levels of HSC70B were beneficial to JEV infection in mosquitoes. Taken together, these findings show that defensin and HSC70B axis facilitates JEV infection in the mosquito.
Asunto(s)
Culex/virología , Defensinas/genética , Regulación hacia Abajo , Virus de la Encefalitis Japonesa (Especie)/patogenicidad , Proteínas HSP70 de Choque Térmico/metabolismo , Interacciones Huésped-Patógeno/genética , Proteínas de la Membrana/metabolismo , Animales , Antivirales/metabolismo , Células Cultivadas , Femenino , Mosquitos Vectores/virología , Internalización del VirusRESUMEN
Japanese encephalitis virus (JEV) is a viral zoonosis that can cause viral encephalitis, death, and disability. Although the Culex mosquito is the primary vector of JEV, little is known about JEV transmission by this kind of mosquito. Here, we found that mosquito defensin facilitated the adsorption of JEV on target cells via the defensin/lipoprotein receptor-related protein 2 (LRP2) axis. Mosquito defensin bound the ED III domain of the viral envelope (E) protein and directly mediated efficient virus adsorption on the target cell surface; the receptor LRP2, which is expressed on the cell surface, affected defensin-dependent adsorption. As a result, mosquito defensin enhanced JEV infection in the salivary gland, increasing the possibility of viral transmission by mosquitoes. These findings demonstrate the novel role of mosquito defensin in JEV infection and the mechanisms through which the virus exploits mosquito defensin for infection and transmission.IMPORTANCE In this study, we observed the complex roles of mosquito defensin in JEV infection; mosquito defensin exhibited a weak antiviral effect but strongly enhanced binding. In the latter, defensin directly binds the ED III domain of the viral E protein and promotes the adsorption of JEV to target cells by interacting with lipoprotein receptor-related protein 2 (LRP2), thus accelerating virus entry. Together, our results indicate that mosquito defensin plays an important role in facilitating JEV infection and potential transmission.
Asunto(s)
Culex/genética , Defensinas/genética , Virus de la Encefalitis Japonesa (Especie)/genética , Proteínas de Insectos/genética , Proteína 2 Relacionada con Receptor de Lipoproteína de Baja Densidad/genética , Mosquitos Vectores/genética , Proteínas del Envoltorio Viral/genética , Adsorción , Animales , Culex/virología , Defensinas/metabolismo , Virus de la Encefalitis Japonesa (Especie)/metabolismo , Encefalitis Japonesa/transmisión , Encefalitis Japonesa/virología , Regulación de la Expresión Génica , Interacciones Huésped-Patógeno/genética , Humanos , Proteínas de Insectos/metabolismo , Proteína 2 Relacionada con Receptor de Lipoproteína de Baja Densidad/metabolismo , Mosquitos Vectores/virología , Unión Proteica , Glándulas Salivales/metabolismo , Glándulas Salivales/virología , Proteínas del Envoltorio Viral/metabolismo , Internalización del VirusRESUMEN
Japanese Encephalitis virus (JEV) is a zoonotic flavivirus that represents the most significant etiology of childhood viral neurological infections throughout the Asia. During the last 20 years, JEV genotype dominance has shifted from genotype III (GIII) to genotype I (GI). To date, the exact mechanism of this displacement is still not known. Culex (Cx.) mosquitoes are the most common species in China and play an essential role in maintaining JEV enzootic transmission cycle. In this study, we used Cx. pipiens mosquitoes from China as an in vivo mosquito model to explore if mosquitoes played a potential role in JEV genotype shift. We exposed female Cx. pipiens mosquitoes orally to either GI or GIII JEV strains. Midgut, whole mosquitoes, secondary organs, and salivary glands of JEV-infected mosquitoes were collected at 7 and 14 days of post infection (dpi) and subjected to measure the infection rate, replication kinetics, dissemination rate and transmission potential of the infected JEV strains in Cx. pipiens mosquitoes by 50% tissue culture infective dose assay. We found that Cx. pipiens mosquito was competent vector for both GI and GIII JEV infection, with similar infection rates and growth kinetics. After the establishment of infection, Cx. pipiens mosquitoes disseminated both JEV genotypes to secondary organs at similar rates of dissemination. A few GI-infected mosquito salivary glands (16.2%) were positive for GI virus, whereas GIII virus was undetectable in GIII-infected mosquito salivary glands at 7 dpi. However, 29.4% (5/17) and 36.3% (8/22) were positive for GI- and GIII-infected mosquito salivary glands at 14 dpi, respectively, showing an increase in JEV positive rate. No statistical difference in the transmission rate between GI- and GIII-infected mosquitoes was detected. Our experiment data demonstrated that GI and GIII viruses have similar infectivity in Cx. pipiens mosquitoes, suggesting that Cx. pipiens mosquitoes from China may not play a critical role in JEV genotype shift. Although the current data were obtained solely from Cx. pipiens mosquitoes, it is likely that the conclusion drawn could be extrapolated to the role of mosquitoes in JEV genotype shift.
Asunto(s)
Culex/virología , Virus de la Encefalitis Japonesa (Especie)/genética , Animales , China , Virus de la Encefalitis Japonesa (Especie)/crecimiento & desarrollo , Encefalitis Japonesa/transmisión , Encefalitis Japonesa/virología , Femenino , Tracto Gastrointestinal/virología , Genotipo , Mosquitos Vectores/virología , Glándulas Salivales/virologíaRESUMEN
BACKGROUND: Acinetobacter baumannii is ubiquitous, facultative intracellular, and opportunistic bacterial pathogen. Its unique abilities allow it to survive in a diverse range of environments, including health care settings, leading to nosocomial infections. And its exceptional ability to develop resistance to multiple antibiotics leaves few drug options for treatment. It has been recognized as a leading cause of nosocomial pneumonia and bacteremia over the world. CASE PRESENTATION: In this case, a 73-year-old woman presented with a Neer Group VI proximal humeral fracture. Six hours after a successfully performed hemiarthroplasty, she developed continuous fever. Clinical examination revealed that the vitals were regular. Laboratory and radiographic examinations revealed only elevated procalcitonin levels. Blood culture revealed no bacterial or fungal growth. Cooling treatment and empirical broad-spectrum antibiotic therapy showed no apparent effect. CONCLUSIONS: We report a postoperative infection caused by Acinetobacter baumannii. The infectious pathogen was identified via molecular DNA sequencing and was initially misidentified as a free-living amoeba species upon microscopic examinations. The patient was mistreated with antiamebic combination therapy. Her symptoms persisted for over 4 months and were eventually followed by her death.
Asunto(s)
Infecciones por Acinetobacter/diagnóstico , Acinetobacter baumannii/aislamiento & purificación , Infecciones por Acinetobacter/microbiología , Anciano , Amebiasis/diagnóstico , Amebiasis/parasitología , Antiprotozoarios/uso terapéutico , Infección Hospitalaria , Errores Diagnósticos , Resultado Fatal , Femenino , Humanos , Análisis de Secuencia de ADN , Tubulinos/fisiologíaRESUMEN
Japanese encephalitis (JE) is a viral encephalitis disease caused by infection with the Japanese encephalitis virus (JEV). The virus can cross the blood-brain barrier and cause death or long-term sequela in infected humans or animals. In this study, we first investigated the distribution of JEV infection in brain and further analyzed the dynamic change in inflammation related genes, chemokines, as well as pathological characteristics. Results demonstrated that CCR2 and CCR5 antagonist could significantly inhibit the inflammation. The mice treated with CCR2 and CCR5 antagonists had a higher survival rate between 60% and 70%, respectively. In summary, our study thoroughly illustrated the characteristics of the dynamic change in inflammation related genes and chemokines induced by JEV infection. We further indicated that CCR5 and CCR2 are potential targets for treatment of JE.
Asunto(s)
Antagonistas de los Receptores CCR5/farmacología , Quimiocinas/metabolismo , Encefalitis Japonesa/tratamiento farmacológico , Encefalitis Japonesa/metabolismo , Inflamación/tratamiento farmacológico , Inflamación/metabolismo , Receptores de Quimiocina/antagonistas & inhibidores , Animales , Barrera Hematoencefálica/metabolismo , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Línea Celular , Chlorocebus aethiops , Citocinas/metabolismo , Modelos Animales de Enfermedad , Virus de la Encefalitis Japonesa (Especie)/efectos de los fármacos , Femenino , Ratones , Ratones Endogámicos C57BL , Receptores CCR2/antagonistas & inhibidores , Receptores CCR5 , Células VeroRESUMEN
Peptidoglycan recognition proteins (PGRPs) and commensal microbes mediate pathogen infection outcomes in insect disease vectors. Although PGRP-LD is retained in multiple vectors, its role in host defense remains elusive. Here we report that Anopheles stephensi PGRP-LD protects the vector from malaria parasite infection by regulating gut homeostasis. Specifically, knock down of PGRP-LD (dsLD) increased susceptibility to Plasmodium berghei infection, decreased the abundance of gut microbiota and changed their spatial distribution. This outcome resulted from a change in the structural integrity of the peritrophic matrix (PM), which is a chitinous and proteinaceous barrier that lines the midgut lumen. Reduction of microbiota in dsLD mosquitoes due to the upregulation of immune effectors led to dysregulation of PM genes and PM fragmentation. Elimination of gut microbiota in antibiotic treated mosquitoes (Abx) led to PM loss and increased vectorial competence. Recolonization of Abx mosquitoes with indigenous Enterobacter sp. restored PM integrity and decreased mosquito vectorial capacity. Silencing PGRP-LD in mosquitoes without PM didn't influence their vector competence. Our results indicate that PGPR-LD protects the gut microbiota by preventing hyper-immunity, which in turn promotes PM structurally integrity. The intact PM plays a key role in limiting P. berghei infection.
Asunto(s)
Anopheles/parasitología , Proteínas Portadoras/fisiología , Quitina/metabolismo , Microbioma Gastrointestinal/fisiología , Interacciones Huésped-Parásitos , Malaria , Animales , Anopheles/genética , Proteínas Portadoras/genética , Técnicas de Silenciamiento del Gen , Predisposición Genética a la Enfermedad , Homeostasis/fisiología , Interacciones Huésped-Parásitos/genética , Insectos Vectores/parasitología , Malaria/genética , Malaria/microbiología , Malaria/parasitología , Malaria/transmisión , Ratones , Ratones Endogámicos BALB C , Plasmodium berghei/patogenicidad , Plasmodium berghei/fisiologíaRESUMEN
Japanese encephalitis virus (JEV) is an arthropod-borne flavivirus prevalent in Asia and the Western Pacific and is the leading cause of viral encephalitis. JEV is maintained in a transmission cycle between mosquitoes and vertebrate hosts, but the molecular mechanisms by which the mosquito vector participates in transmission are unclear. We investigated the expression of all C-type lectins during JEV infection in Aedes aegypti The C-type lectin mosquito galactose-specific C-type lectin 7 (mosGCTL-7) (VectorBase accession no. AAEL002524) was significantly upregulated by JEV infection and facilitated infection in vivo and in vitro mosGCTL-7 bound to the N-glycan at N154 on the JEV envelope protein. This recognition of viral N-glycan by mosGCTL-7 is required for JEV infection, and we found that this interaction was Ca2+ dependent. After mosGCTL-7 bound to the glycan, mosPTP-1 bound to mosGCTL-7, promoting JEV entry. The viral burden in vivo and in vitro was significantly decreased by mosPTP-1 double-stranded RNA (dsRNA) treatment, and infection was abolished by anti-mosGCTL-7 antibodies. Our results indicate that the mosGCTL-7/mosPTP-1 pathway plays a key role in JEV infection in mosquitoes. An improved understanding of the mechanisms underlying flavivirus infection in mosquitoes will provide further opportunities for developing new strategies to control viral dissemination in nature.IMPORTANCE Japanese encephalitis virus is a mosquito-borne flavivirus and is the primary cause of viral encephalitis in the Asia-Pacific region. Twenty-four countries in the WHO Southeast Asia and Western Pacific regions have endemic JEV transmission, which exposes >3 billion people to the risks of infection, although JEV primarily affects children. C-type lectins are host factors that play a role in flavivirus infection in humans, swine, and other mammals. In this study, we investigated C-type lectin functions in JEV-infected Aedes aegypti and Culex pipiens pallens mosquitoes and cultured cells. JEV infection changed the expression of almost all C-type lectins in vivo and in vitro, and mosGCTL-7 bound to the JEV envelope protein via an N-glycan at N154. Cell surface mosPTP-1 interacted with the mosGCTL-7-JEV complex to facilitate virus infection in vivo and in vitro Our findings provide further opportunities for developing new strategies to control arbovirus dissemination in nature.
Asunto(s)
Aedes/química , Aedes/virología , Culex/química , Culex/virología , Virus de la Encefalitis Japonesa (Especie)/fisiología , Lectinas Tipo C/genética , Lectinas Tipo C/metabolismo , Animales , Línea Celular , Encefalitis Japonesa/fisiopatología , Encefalitis Japonesa/transmisión , Encefalitis Japonesa/virología , Interacciones Huésped-Patógeno , Lectinas Tipo C/química , ARN Bicatenario/farmacología , Proteínas del Envoltorio Viral/metabolismo , Carga Viral , Internalización del VirusRESUMEN
ß-carbonic anhydrases (ß-CAs) are ubiquitous metalloenzymes which active site contains a zinc ion (Zn²âº), and they could catalyze the hydration of carbon dioxide to bicarbonate and protons efficiently and are involved in many biological processes, such as respiration, pH and CO2 homeostasis, biosynthetic reactions, virulence regulation and so on, and may play a critical role in the life activity of many organisms which contain these enzymes. ß-CAs are widely distributed in fungi, bacteria, algae, plants and a small number of protozoan and metazoan except vertebrates. Therefore, as potential drug targets for designing and developing antibacterial and anti-parasitic drugs, ß-CAs promise a broad application prospect. This paper focuses on the distribution, physiological function and the progress of researches on ß-CAs in parasites and their vectors.
Asunto(s)
Antiparasitarios/farmacología , Inhibidores de Anhidrasa Carbónica/farmacología , Anhidrasas Carbónicas/fisiología , Animales , Diseño de Fármacos , HumanosRESUMEN
Splenic ameboma (amebic granuloma) is rare. A 38-year-old man with a severe pneumonia due to inhalation of swimming pool water 18 months ago complained of night sweat, tiredness, and anorexia for 1 month. Abdominal ultrasound showed multiple nodules in the spleen. These nodules showed slight enhancement on enhanced CT and intense FDG uptake on PET/CT. Splenectomy was performed. Multiple splenic amebomas were confirmed by pathology. Free-living amoebae were also detected in samples of pleural effusion and blood. Splenic ameboma, although rare, should be included in the differential diagnosis with isolated focally increased FDG activity in the spleen.
Asunto(s)
Amebiasis/diagnóstico por imagen , Granuloma/diagnóstico por imagen , Tomografía Computarizada por Tomografía de Emisión de Positrones , Bazo/diagnóstico por imagen , Adulto , Diagnóstico Diferencial , Fluorodesoxiglucosa F18 , Granuloma/parasitología , Humanos , Masculino , Radiofármacos , Bazo/patologíaRESUMEN
Objective: To identify the species of a morphologically Acanthamoeba-like pathogen in sputum from a patient with repeated cough. Methods: Protozoa were isolated from the sputum and cultured for morphological observation of the trophozoites and cysts. DNA was extracted from the cultivated sample, and PCR was performed using primers as follows: 18S universal primers for amoeba familyï¼Ami6F1 and Ami9Rï¼ and for amoeba genusï¼JDP1 and JDP2ï¼, and primers for 18S full-length sequence of S-7 ATCC reference strain of Acanthamoeba griffini ï¼AacGF and AscGRï¼. The 18S rRNA was sequenced, followed by homology analysis. The maximum likelihood method was used to construct phylogenetic tree. Results: Microscopic examination showed that the trophozites had spine and irregular-shape pseudopodia bulge. The cysts were encapsulated by double membrane layer with the inner membrane having star-like processes. As expected, PCR amplification resulted in bands of 830, 479 and 1 957 bp, respectively, which were blasted to be 99%, 99% and 100% homologous to those of A. griffiniï¼U07412.1ï¼. Phylogenetic tree indicated that this acanthamobe in the patient's sample was 91.4%, 99.6%, 94.5% and 91.8% homologous to keratitis-associated A. castellanii, A. polyphage, A. cullbertsoni and A. rhysodes. Conclusion: The parasite in sputum of the patient with respiratory tract infection is Acanthamoeba griffini.
Asunto(s)
Acanthamoeba , Filogenia , Animales , Secuencia de Bases , Cartilla de ADN , ADN Protozoario , Humanos , Reacción en Cadena de la Polimerasa , ARN Ribosómico 18S , Infecciones del Sistema RespiratorioRESUMEN
An imported case previously misdiagnosed as vivax malaria was reviewed. The epidemiological data and blood sample were collected. The detection was conducted by microscopy, rapid diagnostic test (RDT) and nested PCR. The case was finally comfirmed as the first imported case of Plasmodium ovale infection in Nanping.
Asunto(s)
Malaria/diagnóstico , Plasmodium ovale , China , Humanos , Malaria Vivax , Microscopía , Reacción en Cadena de la PolimerasaRESUMEN
The incidence of opportunistic parasitic infections is increasing as a result of the growing population with immune deficiency. Currently, studies on opportunistic parasitic infections are limited by the lack of animal models, due to the limited biological knowledge on the opportunistic parasitic pathogens as well as the small number of studies on species identification and typing, epidemiologic status, as well as the source and route of infection. The prevalence of HIV has promoted the research and understanding of opportunistic parasitic infections, which, in turn, has greatly reduced the morbidity and mortality of opportunistic infections. However, there still exists a bottleneck for the control and prevention of infectious diseases, i.e., the lack of sensitive and specific diagnostic methods and effective therapy, due to the complicated clinical manifestations and insufficient notification by clinical physicians. Supported by accumulating basic research, the discovery of diagnostic and therapeutic molecular targets is the key to overcome these problems.
Asunto(s)
Infecciones/parasitología , Animales , Humanos , PrevalenciaRESUMEN
Staphylococcus aureus protein A (SpA) and protein G of groups C and G streptococci (SpG) are two well-defined bacterial immunoglobulin (Ig)-binding proteins (IBPs) with high affinity for specific sites on IgG from mammalian hosts. Both SpA and SpG contain several highly-homologous IgG-binding domains, each of which possesses similar binding characteristic of the whole corresponding proteins. Whether specific combinations of these domains could generate a molecule with novel IgG-binding properties remained unknown. We constructed a combinatorial phage library displaying randomly-rearranged A, B, C, D and E domains of SpA as well as the B2 (G2) and B3 (G3) domains of SpG. In vitro molecular evolution directed by human, rabbit, bovine, or goat polyclonal IgGs and four subclasses of mouse monoclonal IgGs generated one common combination, D-C-G3. A series of assays demonstrated that D-C-G3 exhibited a potential novel IgG binding property that was obviously different from those of both parent proteins. This study provides an example of successful protein engineering through in vitro molecular evolution and useful approaches for structure and function studies of IBPs.
Asunto(s)
Anticuerpos Monoclonales de Origen Murino/aislamiento & purificación , Proteínas Bacterianas/genética , Evolución Molecular Dirigida , Inmunoglobulina G/química , Proteína Estafilocócica A/genética , Secuencia de Aminoácidos , Proteínas Bacterianas/biosíntesis , Cromatografía de Afinidad , Expresión Génica , Inmunoglobulina G/aislamiento & purificación , Datos de Secuencia Molecular , Biblioteca de Péptidos , Unión Proteica , Proteína Estafilocócica A/biosíntesisRESUMEN
Based on the variable part of mtDNA CO I gene sequence, a multiplex PCR method was developed for the identification of the three common sandflies (Phlebotomus longiductus, Ph. wui, and Ph. alexandri) in southern Xinjiang. The results demonstrated that this multiplex PCR method was reliable, and could be used to identify the three Phlebotomus species. The PCR product of CO I gene from Ph. longiductus, Ph. wui and Ph. alexandri was 248, 632, and 395 bp, respectively.
Asunto(s)
Phlebotomus/genética , Animales , ADN Mitocondrial , Reacción en Cadena de la Polimerasa Multiplex , Reacción en Cadena de la PolimerasaRESUMEN
AL (SpA A domain-PpL B3 domain), LD5 (PpL B3 domain-SpA D domain-PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L-D-L) and LD3 (PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L) are novel evolved Ig binding molecules (NEIBMs) derived from the in vitro molecular evolution of combinatorial phage libraries displaying randomly rearranged Ig-binding domains of protein A and protein L. These molecules all showed novel Ig-binding properties of double-site binding to the VH3 and Vκ regions of human Ig Fab and high affinity for human IgM, which enhanced IgM detection in the anti-HCV ELISA assay. In this double-site binding, the A domain binds to the VH3 chain with low affinity. Whether the appropriate mutations in the A domain could improve this binding remains unknown. In this study, four combinatorial phage libraries displaying AL mutants with random mutations at different amino acid positions in the A domain were constructed. Seven AL mutant phages with significantly improved Ig binding activity were obtained from the phage library displaying AL mutants randomly mutated at positions 27 and 34 through human IgM-directed in vitro evolution. Two of the seven prokaryotically expressed AL mutants, AL (VV) and AL (KA), exhibited IgM and IgG binding activities equivalent to those of wild-type AL, whereas other mutants showed attenuated binding. However, after labeling with HRP, AL (VV) and AL (KA) showed improved IgM and IgG binding activity, which significantly improved the detection in the anti-HCV assay. Thus, the present study demonstrates that the binding properties of AL were successfully improved through phage-based molecular evolution, which could substantially contribute to the use of AL in antibody detection, and provides an example of successful protein engineering through in vitro molecular evolution.
Asunto(s)
Evolución Molecular Dirigida , Anticuerpos contra la Hepatitis C/aislamiento & purificación , Inmunoglobulina G/inmunología , Inmunoglobulina M/genética , Bacteriófagos/genética , Anticuerpos contra la Hepatitis C/genética , Anticuerpos contra la Hepatitis C/inmunología , Humanos , Inmunoglobulina G/genética , Inmunoglobulina M/inmunología , Región Variable de Inmunoglobulina/genética , Región Variable de Inmunoglobulina/inmunología , Unión Proteica/genética , Unión Proteica/inmunología , Ingeniería de Proteínas , Proteína Estafilocócica A/genética , Proteína Estafilocócica A/inmunologíaRESUMEN
Culexpipiens quinquefasciatus (C. quinquefasciatus) is an important vector that can transmit human diseases such as West Nile virus, lymphatic filariasis, Japanese encephalitis and St. Louis encephalitis. However, very limited research concerning the humoral and cellular immune defenses of C. quinquefasciatus has been done. Here we present the research on hemocyte identification and plasma including hemocyte prophenoloxidase from C. quinquefasciatus at all developmental stages in order to obtain a complete picture of C. quinquefasciatus innate immunity. We identified hemocytes into four types: prohemocytes, oenocytoids, plasmatocytes and granulocytes. Prophenoloxidase (PPO) is an essential enzyme to induce melanization after encapsulation. PPO-positive hemocytes and plasma PPO were observed at all developmental stages. As for specific hemocyte types, prophenoloxidase was found in the plasmatocytes at larval stage alone and in the smallest prohemocytes during almost all developmental stages. Moreover, the granulocytes were PPO-positive from blood-fed female mosquitoes and oenocytoids were observed PPO-positive in pupae and in adult females after blood-feeding. As for plasma, there were different patterns of PPO in C. quinquefasciatus at different developmental stages. These results are forming a basis for further studies on the function of C. quinquefasciatus hemocytes and prophenoloxidase as well as their involvement in fighting against mosquito-borne pathogens.
Asunto(s)
Catecol Oxidasa/análisis , Culex/citología , Culex/enzimología , Precursores Enzimáticos/análisis , Hemocitos/clasificación , Insectos Vectores/citología , Insectos Vectores/enzimología , Animales , Recuento de Células , Culex/crecimiento & desarrollo , Electroforesis en Gel de Poliacrilamida , Femenino , Hemocitos/citología , Hemocitos/enzimología , Insectos Vectores/crecimiento & desarrollo , Larva/citología , Larva/enzimología , Masculino , Monofenol Monooxigenasa/análisis , Pupa/citología , Pupa/enzimologíaRESUMEN
This paper reports the rectification results of the tribe aedini mosquitoes formerly recorded in China, using the classification system proposed by Reinert during the recent years. Among all the 171 species of Chinese aedini mosquitoes examined, 160 species could be included in the new classification system. The other 11 species were listed in traditional taxonomic status for further study. The proposed new classification system of the Chinese aedini mosquitoes contained 29 genera, i.e. Aedes, Armigeres, Ayurakitia, Bothaella*, Bruceharrisonius*, Christophersiomyia*, Collessius*, Danielsia*, Downsiomyia*, Edwardsaedes*, Finlaya*, Fredwardsius*, Gilesius*, Heizmannia, Himalaius*, Hopkinsius*, Hulecoeteomyia*, Jihlienius*, Kenknightia*, Luius*, Mucidus*, Neomelaniconion*, Ochlerotatus, Phagomyia*, Scutomyia*, Stegomyia*, Tanakaius*, Udaya, and Verrallina. Among them, 22 genera (*) were new records in China. Besides, the authors made a significant revision to the following 4 species recorded formerly in "Fauna Sinica, Insecta Vol. 8, Diptera: Culicidae": Ae. (Edw.) antuensis as the synonym of Ed. pingpaensis, while Ae. (Sin.) occidentayunnanus, Ae. (Och.) flavidorsalis, and Ae. (Fin.) subsimilis should be rectified as Hz. (Mat.) occidentayunnana, Oc. albineus, and Ud. subsimilis, respectively.
Asunto(s)
Aedes/clasificación , Animales , ChinaRESUMEN
The isolation and culture of pathogenic free-living amoebae are useful in the diagnosis and research. This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.
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Acanthamoeba/crecimiento & desarrollo , Acanthamoeba/aislamiento & purificación , Acanthamoeba/patogenicidad , Amoeba/crecimiento & desarrollo , Amoeba/aislamiento & purificación , Amoeba/patogenicidad , Animales , HumanosRESUMEN
Large-scale hydroprojects have a propensity for incurring schistosomiasis epidemics by altering the environments of their vicinities. As the construction of the Three Gorges Dam, one of the world's largest hydroprojects to date, draws near its conclusion, an assessment of the dam's capacity in causing schistosomiasis becomes more urgent and pressing. This article reviews recent investigations into the possible effects of the dam on schistosomiasis in the Three Gorges region and areas along the Yangtze downstream from the dam. Data used in this article were extracted from peer-reviewed papers found in PubMed, Chinese Journal of Parasitology and Parasitic Diseases, and Chinese Journal of Schistosomiasis Control. Results indicate that the Three Gorges Dam is capable of inducing a wide variety of environmental and ecological changes both within the Three Gorges region and in downstream areas. These changes, however, carry ambivalent implications for the reproduction of Oncomelania snails and the spreading of schistosome infections. Furthermore, major changes in the demographics and agricultural practices of the Three Gorges and downstream Yangtze areas caused by the dam could also exert significant influence on the transmission of schistosomiasis in these regions. Major conclusions of this review include the need for further ecological simulations of the Three Gorges Dam and the need for deploying monitoring and intervention systems to provide successful prophylaxis of the Three Gorges Dam-associated schistosomiasis emergence.