Tailoring silk fibroin fibrous architecture by a high-yield electrospinning method for fast wound healing possibilities.

In this study, a novel array electrospinning collector was devised to generate two distinct regenerated silk fibroin (SF) fibrous membranes: ordered and disordered. Leveraging electrostatic forces during the electrospinning process allowed precise control over the orientation of SF fiber, resulting in the creation of membranes comprising both aligned and randomly arranged fiber layers. This innovative approach resulted in the development of large-area membranes featuring exceptional stability due to their alternating patterned structure, achievable through expansion using the collector, and improving the aligned fiber membrane mechanical properties. The study delved into exploring the potential of these membranes in augmenting wound healing efficiency. Conducting in vitro toxicity assays with adipose tissue-derived mesenchymal stem cells (AD-MSCs) and normal human dermal fibroblasts (NHDFs) confirmed the biocompatibility of the SF membranes. We use dual perspectives on exploring the effects of different conditioned mediums produced by cells and structural cues of materials on NHDFs migration. The nanofibers providing the microenvironment can directly guide NHDFs migration and also affect the AD-MSCs and NHDFs paracrine effects, which can improve the chemotaxis of NHDFs migration. The ordered membrane, in particular, exhibited pronounced effectiveness in guiding directional cell migration. This research underscores the revelation that customizable microenvironments facilitated by SF membranes optimize the paracrine products of mesenchymal stem cells and offer valuable physical cues, presenting novel prospects for enhancing wound healing efficiency.

Effects of magnesium hydroxide morphology on Pb(ii) removal from aqueous solutions.

In this study, magnesium hydroxide (MH) particles with distinct morphologies were obtained through direct precipitation and subsequent hydrothermal treatment with various magnesium salts. The synthesized products were systematically characterized and utilized for the removal of Pb(ii) ions from aqueous solutions. The adsorption process of Pb(ii) by two different MH structures, namely flower globular magnesium hydroxide (FGMH) and hexagonal plate magnesium hydroxide (HPMH), adhered to the Langmuir isotherm and pseudo-second-order model. FGMH exhibited higher Pb(ii) removal capacity (2612 mg g-1) than HPMH (1431 mg g-1), attributable to the unique three-dimensional layered structures of FGMH that provide a larger surface area and abundant active sites. Additionally, metallic Pb was obtained by recycling the adsorbed Pb(ii) through acid dissolution-electrolysis. Furthermore, Pb(ii) removal mechanisms were investigated by analyzing adsorption kinetics and isotherms, and the adsorbed products were characterized. Based on the findings, the removal process occurs in two key stages. First, Pb(ii) ions bind with OH- ions on the surface upon diffusing to the MH surface, resulting in Pb(OH)2 deposits in situ. Concurrently, Mg(ii) ions diffuse into the solution, substituting Pb(ii) ions in the MH lattice. Second, the resultant Pb(OH)2, which is unstable, reacts with CO2 dissolved in water to yield Pb3(CO3)2(OH)2. Therefore, owing to its outstanding Pb(ii) adsorption performance and simple preparation method, FGMH is a promising solution for Pb(ii) pollution.

An adaptive algorithm for generating 3D point clouds of the human body based on 4D millimeter-wave radar.

The traditional algorithms for generating 3D human point clouds often face challenges in dealing with issues such as phantom targets and target classification caused by electromagnetic multipath effects, resulting in a lack of accuracy in the generated point clouds and requiring manual labeling of the position of the human body. To address these problems, this paper proposes an adaptive method for generating 3D human point clouds based on 4D millimeter-wave radar (Self-Adaptive mPoint, SA-mPoint). This method estimates the rough human point cloud by considering micro-motion and respiration characteristics while combining the echo dynamic with static information. Furthermore, it enhances the density of point cloud generation. It reduces interference from multipath noise through multi-frame dynamic fusion and an adaptive density-based clustering algorithm based on the center points of humans. The effectiveness of the SA-mPoint algorithm is verified through experiments conducted using the TI Millimeter Wave Cascade Imaging Radar Radio Frequency Evaluation Module 77G 4D cascade radar to collect challenging raw data consisting of single-target and multi-target human poses in an open classroom setting. Experimental results demonstrate that the proposed algorithm achieves an average accuracy rate of 97.94% for generating point clouds. Compared to the popular TI-mPoint algorithm, it generates a higher number of point clouds on average (increased by 87.94%), improves the average accuracy rate for generating point clouds (increased by 78.3%), and reduces the running time on average (reduced by 11.41%). This approach exhibits high practicality and promising application prospects.

Use of Compressed Sensing Accelerated, Low-Velocity Encoded, Isotropic Resolution, Phase Contrast Magnetic Resonance Angiography for SEEG Electrode Implantation.

OBJECTIVE: We assessed the feasibility of using compressed sensing accelerated, low-velocity encoded, isotropic resolution phase contrast (CLIP) magnetic resonance angiography (MRA) for avascular trajectory planning of stereoelectroencephalography. METHODS: Ten healthy subjects (1 woman and 9 men; age, 33.6 ± 9.0 years) and 20 consecutive patients (12 female patients; age, 22 ± 13.6 years) were enrolled in the present study. The healthy subjects underwent CLIP-MRA, and 3 other phase contrast MRA protocols with conventional parallel imaging (PI) acceleration, including low resolution with twofold PI (PI2), high resolution (HR) with fivefold PI (PI5), and HR-PI2. The patients underwent CLIP-MRA and computed tomography angiography (CTA). The image qualities were evaluated. The numbers and locations of trajectory-vessel conflict detected using CLIP-MRA were noted. RESULTS: With similar scan durations, CLIP-MRA achieved higher spatial resolution compared with low resolution with PI2 and detected significantly more branches compared with HR-PI5. With the same spatial resolution, the signal/noise and contrast/noise ratios of CLIP-MRA were higher than those with HR-PI2 with a shorter scan duration. For the 12 adult patients (10 female patients; 28.8 ± 12.7 years), CLIP-MRA had better signal/noise and contrast/noise ratios than CTA. The trajectory had required modification for 14 of the 20 patients (70%), with a proportion of trajectory modification of 10.7% (23 of 215 electrodes). The middle meningeal artery, cortical vessel, and skull vessel were the main vessels with conflict (n = 11, n = 7, and n = 5, respectively). CONCLUSIONS: In the present study, CLIP-MRA provided a clear cortical vascular display noninvasively without intravascular contrast and radiation. The middle meningeal artery and diploic and emissary veins were the main conflict vessels and could be clearly displayed using CLIP-MRA but not CTA.

Facile fabrication of Fe3O4@Mg(OH)2 magnetic composites and their application in Cu(ii) ion removal.

In this study, we fabricated magnetic Fe3O4@Mg(OH)2 composites through the seed deposition technique to achieve Cu(ii) ion removal from aqueous solutions. As indicated by the characterization results, three-dimensional flower-like spheres composed of external Mg(OH)2 were formed, with nano-Fe3O4 particles uniformly embedded in the "flower petals" of the spheres. The efficacy of Fe3O4@Mg(OH)2-3 in Cu(ii) ion removal was examined through batch experiments. The impact of solution pH on removal efficiency was examined, and the pseudo-second-order model and the Langmuir model provided good fits to the adsorption kinetics and isotherm data, respectively. Remarkably, Fe3O4@Mg(OH)2-3 exhibited a significant removal capacity of 1051.65 mg g-1 for Cu(ii) ions. Additionally, the composite displayed a notable saturation magnetization value of 17.3 emu g-1, facilitating isolation from sample solutions through external magnetic fields after Cu(ii) ion absorption. At the solid-liquid interface, a mechanism involving ion exchange between Mg(ii) and Cu(ii) cations was realized as the mode of Cu(ii) ion removal. The composites' effective adsorption properties and rapid magnetic separation highlighted their suitability for use in treating copper-contaminated water.

Enhancing degradation of organic matter in microbial electrolytic cells coupled with anaerobic digestion (MEC-AD) systems by carbon-based materials.

Currently, little information is available on relative contributions among biochar (BC), activated carbon (AC), magnetic BC (MBC), and magnetic AC (MAC) to enhance the effectiveness of a microbial electrolytic cells coupled with anaerobic digestion (MEC-AD) system and the impact of carbon-based materials on microbial community. In this study, six anaerobic reactors were constructed to demonstrate the effects of different carbon-based materials on organic matter elimination in the MEC-AD system. Remarkably, the reactor containing MBC exhibited a significant increase in organic removal, achieving 95.0 % chemical oxygen demand (COD) eradication. Additionally, the MBC-added MEC-AD reactor yields acetic acid at a rate 2.9 times higher than that of the BC-added reactor. Electrical stimulation enriched electro-producing bacteria such as Pseudomonas (18.1 %) and Gordonia (6.8 %), which were further promoted by the addition of MBC, indicating that the microbial communities cultivated with the MBC could provide the necessary microbiome for the MEC.

Chromosome-level genome assembly of Chouioia cunea Yang, the parasitic wasp of the fall webworm.

Chouioia cunea Yang 1989 is a parasitic wasp of many lepidopteran insects during their pupal stage, and has been successfully used to control pests such as the fall webworm Hyphantria cunea. Here we reported the chromosome-level genome of C. cunea by using short (MGI-SEQ), long (Oxford Nanopore), chromatin-linked (Hi-C) sequencing reads and transcriptomic data, representing the first chromosome-level genome of parasitic wasps of the family Eulophidae. The total assembly length is 171.99 Mb, containing 6 pesudo-chromosomes with a GC content of 36.89% and the scaffold/contig N50 length of 31.70/26.52 Mb. The BUSCO completeness of the assembly was estimated to be 98.7%. A total of 12,258 protein-coding genes (PCGs), 10,547 3'-UTRs, and 10,671 5'-UTRs were annotated. This high-quality genome is an important step toward a better understanding of the genomes of the Eulophidae (Chalcidoidea), and will serve as a valuable resource for analyses of phylogenetic relationships and the evolution of Hymenoptera.

Evolutionary timescale of chalcidoid wasps inferred from over one hundred mitochondrial genomes.

Chalcidoidea is one of the most biologically diverse groups among Hymenoptera. Members are characterized by extraordinary parasitic lifestyles and extensive host ranges, among which several species attack plants or serve as pollinators. However, higher-level chalcidoid relationships remain controversial. Here, we performed mitochondrial phylogenomic analyses for major clades (18 out of 25 families) of Chalcidoidea based on 139 mitochondrial genomes. The compositional heterogeneity and conflicting backbone relationships in Chalcidoidea were assessed using various datasets and tree inferences. Our phylogenetic results supported the monophyly of 16 families and polyphyly of Aphelinidae and Pteromalidae. Our preferred topology recovered the relationship (Mymaridae+(Signiphoridae+Leucospidae)+(Chalcididae+((Perilampidae+Eucharitidae)+ remaining Chalcidoidea)))). The monophyly of Agaonidae and Sycophaginae was rejected, while the gall-associated ((Megastigmidae+Ormyridae)+(Ormocerinae+Eurytomidae)) relationship was supported in most results. A six-gene inversion may be a synapomorphy for most families, whereas other derived gene orders may introduce confusion in phylogenetic signals at deeper nodes. Dating estimates suggested that Chalcidoidea arose near the Jurassic/Cretaceous boundary and that two dynamic shifts in diversification occurred during the evolution of Chalcidoidea. We hypothesized that the potential codiversification between chalcidoids and their hosts may be crucial for accelerating the diversification of Chalcidoidea. Ancestral state reconstruction analyses supported the hypothesis that gall-inducers were mainly derived from parasitoids of gall-inducers, while other gall-inducers were derived from phytophagous groups. Taken together, these findings advance our understanding of mitochondrial genome evolution in the major interfamilial phylogeny of Chalcidoidea.

The Mitochondrial Genomes of Two Parasitoid Wasps Protapanteles immunis and Parapanteles hyposidrae (Hymenoptera: Braconidae) with Phylogenetic Implications and Novel Gene Rearrangements.

Parapanteles hypsidrae (Wilkinson, 1928) and Protapanteles immunis (Haliday, 1834) are the most important parasitic wasps of Ectropis grisescens Warren and Ectropis obliqua (Prout). We sequenced and annotated the mitochondrial genomes of Pa. hyposidrae and Pr. immunis, which are 17,063 bp and 16,397 bp in length, respectively, and possess 37 mitochondrial genes. We discovered two novel types of gene rearrangement, the local inversion of nad4L in Pa. hyposidrae and the remote inversion of the block cox3-nad3-nad5-nad4 in Pr. immunis, within the mitogenomes of Braconidae. The phylogenetic analysis supported the subfamily Microgastrinae is a monophyletic group, but the tribes Apantelini and Cotesiini within this subfamily are paraphyletic groups.

The genus Eurymeros Bhat (Hymenoptera, Braconidae, Alysiinae) newly recorded from China.

Background: Alysiinae Leach is a species-rich subfamily in Braconidae, of which several species play an important role in biological control. The monotypic genus Eurymerostumespiraculum Bhat, 1980 was discovered in Tibet and Yunnan provinces for the first time, representing the first record of the genus Eurymeros Bhat, 1980 (Braconidae, Alysiinae) in China. New information: The rare genus Eurymeros Bhat, 1980 (Braconidae, Alysiinae) and its only known species, E.tumespiraculum Bhat, 1980, are newly recorded from China. The morphological variation of the Chinese specimens is described and illustrated.

Membrane Applications in Autologous Cell Therapy.

Stem cell and cell therapies, particularly autologous cell therapies, are becoming a common practice. However, in order for these technologies to achieve wide-scale clinical application, the prohibitively high cost associated with these therapies must be addressed through creative engineering. Membranes can be a disruptive technology to reshape the bioprocessing and manufacture of cellular products and significantly reduce the cost of autologous cell therapies. Examples of successful membrane applications include expansions of CAR-T cells, various human stem cells, and production of extracellular vesicles (EVs) using hollow fibre membrane bioreactors. Novel membranes with tailored functions and surface properties and novel membrane modules that can accommodate the changing needs for surface area and transport properties are to be developed to fulfil this key role.

An artificial LAMA2-GelMA hydrogel microenvironment for the development of pancreatic endocrine progenitors.

The biomimetic pancreatic microenvironment improves the differentiation efficiency and function of human embryonic stem cell-derived ß-cells (SC-ß cells). Thus, a laminin subunit alpha 2-gelatin methacrylate (LAMA2-GelMA) hybrid hydrogel as a bionics carrier for the formation and maturation of endocrine lineage was developed in our research, based on pancreas proteomics analysis of postnatal mice. Pancreatic endocrine cells cultured on the hybrid hydrogel in vitro, which was composed of 0.5 µg/mL LAMA2 protein and 4% GelMA, the expression of transcription factors (TFs), including NKX6.1, NKX6.2, and NEUROD1 were upregulated. Single-cell transcriptomics was performed after LAMA2 knockdown during the early differentiation of pancreatic progenitor (PP) cells, a marked decrease in the forkhead box protein A2 (FOXA2+)/GATA-binding factor 6 (GATA6+) cluster was detected. Also, we clarified that as a receptor of LAMA2, integrin subunit alpha 7 (ITGA7) participated in Integrin-AKT signaling transduction and influenced the protein levels of FOXA2 and PDX1. In vivo experiments showed that, PP cells encapsulated in the LAMA2-GelMA hydrogel exhibited higher serum C-peptide levels compared to the GelMA and Matrigel groups in nude mice and reversed hyperglycemia more quickly in STZ-induced diabetic nude mice. Taken together, our findings highlighted the feasibility of constructing a pancreas-specific microenvironment based on proteomics and tissue engineering for the treatment of diabetes.

Organic-Inorganic Modification of Magnesium Borate Rod by Layered Double Hydroxide and 3-Aminopropyltriethoxysilane and Its Effect on the Properties of Epoxy Resin.

To alleviate the safety hazards associated with the use of epoxy resin (EP), a multifunctional filler was designed. This study firstly combines the superior mechanical properties of magnesium borate rods (MBR) with the excellent smoke suppression and flame-retardant characteristics of layered double hydroxide (LDH). H2PO4- intercalated LDH (LDHP) was coated on the MBR surface to obtain inorganic composite particles MBR@LDHP. Subsequently, MBR@LDHP was modified with 3-aminopropyltriethoxysilane (APES) to obtain organic-inorganic composite particles MBR@LDHP-APES. Eventually, the hybrid particles were added to EP to prepare the composite materials. Thereafter, the morphology, composition, and structure of MBR@LDHP-APES were characterized utilizing scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD). The results indicated the successful preparation of MBR@LDHP-APES, after which we investigated the effects of MBR@LDHP-APES on the smoke suppression, flame retardancy, and mechanical characteristics of EP. As observed, the EP composites containing 7.5 wt% MBR@LDHP-APES exhibited superior smoke suppression and flame retardancy abilities. The limiting oxygen index reached 33.5%, which is 36.73% greater than pure EP, and the lowest values of total heat and smoke release were observed for the composite materials. In addition, the mechanical properties test revealed that MBR@LDHP-APES considerably enhanced the tensile strength as well as the flexural strength of the composites. Furthermore, mechanistic studies suggested that the barrier effect of MBR, endothermic decomposition of LDHP, and the synergistic effect of LDHP and APES contributed essentially to the smoke suppression and flame-retardant properties of the material. The findings of this research point to a potential method for enhancing the EP's ability to suppress smoke and flames while enhancing its mechanical properties.

The Dual Functions of a Bracovirus C-Type Lectin in Caterpillar Immune Response Manipulation.

Parasitoids are widespread in natural ecosystems and normally equipped with diverse viral factors to defeat host immune responses. On the other hand, parasitoids can enhance the antibacterial abilities and improve the hypoimmunity traits of parasitized hosts that may encounter pathogenic infections. These adaptive strategies guarantee the survival of parasitoid offspring, yet their underlying mechanisms are poorly understood. Here, we focused on Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella, and found that C. vestalis parasitization decreases the number of host hemocytes, leading to disruption of the encapsulation reaction. We further found that one bracovirus C-type lectin gene, CvBV_28-1, is highly expressed in the hemocytes of parasitized hosts and participates in suppressing the proliferation rate of host hemocytes, which in turn reduces their population and represses the process of encapsulation. Moreover, CvBV_28-1 presents a classical bacterial clearance ability via the agglutination response in a Ca2+-dependent manner in response to gram-positive bacteria. Our study provides insights into the innovative strategy of a parasitoid-derived viral gene that has dual functions to manipulate host immunity for a successful parasitism.

Preparation and Biocompatibility Study of Contrast-Enhanced Hernia Mesh Material.

BACKGROUND: Meshes play a crucial role in hernia repair. However, the displacement of mesh inevitably leads to various associated complications. This process is difficult to be traced by conventional imaging means. The purpose of this study is to create a contrast-enhanced material with high-density property that can be detected by computed tomography (CT). METHODS: The contrast-enhanced monofilament was manufactured from barium sulfate nanoparticles and medical polypropylene (PP/Ba). To characterize the composite, stress tensile tests and scanning electron microscopy (SEM) was performed. Toxicity and biocompatibility of PP/Ba materials was verified by in vitro cellular assays. Meanwhile, the inflammatory response was tested by protein adsorption assay. In addition, an animal model was established to demonstrate the long-term radiographic effect of the composite material in vivo. Subsequent pathological tests confirmed its in vivo compatibility. RESULTS: The SEM revealed that the main component of the monofilament is carbon. In vitro cell experiments demonstrated that novel material does not affect cell activity and proliferation. Protein adsorption assays indicated that the contrast-enhanced material does not cause additional inflammatory responses. In addition, in vivo experiments illustrated that PP/Ba mesh can be detected by CT and has good in vivo compatibility. CONCLUSION: These results highlight the excellent biocompatibility of the contrast-enhanced material, which is suitable for human abdominal wall tissue engineering.

Gender Differences in Diagnostic Radiology Practice: An Observational Study.

PURPOSE: To investigate gender differences in diagnostic radiology practice, specifically, the differences in scope of practice, the frequency of consultations to other colleagues, and the error rates. MATERIAL AND METHODS: A retrospective observational study was performed including radiologists working for a European teleradiology provider between 2013 and 2019. Main outcome measures included the adjusted odds ratio of female gender for reporting cases in more than one subspecialty, the adjusted incidence rate ratio (IRR) of female gender for the count of second opinion requests to other colleagues, and the adjusted IRR of female gender for the count of radiologic errors. Multivariable adjustment was performed for covariates associated with experience, type of cases reported, part- or full-time employment, and reporting speed. RESULTS: A total of 213 radiologists (36% female) were included in the analysis of gender differences in scope of practice. Female gender was associated with a lower odds of reporting cases in more than one subspecialty with an odds ratio of 0.46 (95% confidence interval, 0.22-0.96). A total of 204 radiologists (36% female) were included in the analysis of gender differences in the count of second opinion requests to colleagues. There was a trend toward an association between female gender and higher odds of requesting a second opinion with an adjusted IRR of 1.6 compared with male gender, but it was not statistically significant (P = .08). A total of 199 radiologists were included (37% female) in the analysis of gender differences in the number of radiologic errors. Female gender was associated with a decrease in the odds of error with an IRR of 0.8 (95% confidence interval, 0.64-0.995). CONCLUSIONS: Female radiologists tend to have a narrower scope of practice and make fewer mistakes than their male counterparts, even after detailed adjustment for factors that might explain gender differences in scope of practice and errors.

Bracoviruses recruit host integrases for their integration into caterpillar's genome.

Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential "8+5" nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar's genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts.

Pancreatic Extracellular Matrix/Alginate Hydrogels Provide a Supportive Microenvironment for Insulin-Producing Cells.

Type 1 diabetes mellitus (T1DM), as an autoimmune deficiency disease, is associated with an absolute deficiency of insulin subject to islet ß-cell destruction. Insulin-producing cells (IPCs) differentiated from induced pluripotent stem cells are an ideal replacement origin of ß-cells, which can be applied for cell transplantation therapies in T1DM. At present, more strategies focus on inducing and differentiating to obtain IPCs; however, the unsatisfactory differentiation efficiency and the lack of ideal carriers for in vivo transplantation limited their application. It is necessary to consider the cell microenvironment by constructing a biomimetic niche to improve the differentiation and transplantation efficiency. The main components of the extracellular matrix derived from pancreatic (the niche of ß-cells) decellularization were retained, which could provide the ideal extracellular microenvironment for IPCs. In this research, a hydrogel prepared with alginate (Alg) and the pancreatic extracellular matrix (pECM) was assessed for the beneficial outcomes on encapsulated IPCs. The results showed that pECM/Alg improved the differentiation efficiency and promoted insulin secretion and the expression of insulin-related genes as well. Besides, pECM/Alg-encapsulated IPCs exhibited obvious biocompatibility in vivo, which can prolong the transplantation effect and hypoglycemic function by reducing the inflammatory reaction. RNA-seq indicated that the PI3K/Akt pathway may be related to the improvement of the differentiation efficiency and function of IPCs. In general, the pECM/Alg hydrogel provides an ideal biomimetic microenvironment for IPCs and is suitable for in vivo transplantation.

Endovascular community response to mortality data in use of paclitaxel devices for peripheral vascular disease.

OBJECTIVE: We examined the endovascular community response to data demonstrating increased mortality in paclitaxel devices for the treatment of peripheral arterial disease in femoropopliteal lesions. METHODS: A retrospective observational study using the Vascular Quality Initiative Peripheral Vascular Intervention registry dataset was performed to examine paclitaxel device use for peripheral arterial disease in femoropopliteal arteries treated from 2017 to 2019. A total of 41,707 patients and 52,208 procedures were analyzed during the study period. A post hoc analysis was performed to examine paclitaxel device use during selected periods in 2019. RESULTS: The total number of femoropopliteal procedures in 2017, 2018, and 2019 were 17,458, 21,140, and 21,322, respectively. Paclitaxel devices were used for 8852 arteries in 2017, 10,691 in 2018, and 6732 in 2019, which was significantly reduced in 2019 compared with 2017 or 2018 (P < .0001) and 2019 compared with the 2018 and 2017 volumes combined (P < .0001). Post hoc analysis of selected periods in 2019 demonstrated variable use throughout 2019. CONCLUSIONS: After the report of data with concerns of mortality associated with paclitaxel device use in 2018, a rapid reduction in overall paclitaxel device use was observed in 2019.

The mitochondrial genome of Telenomus remus (Hymenoptera: Platygastridae).

Telenomus remus Nixon, 1937 is an important parasitoid of lepidopterans. We sequenced the mitochondrial genome of T. remus, 15,500 bp in size, and possessed all 37 typical mitochondrial genes. A few tRNAs show gene arrangements compared with the ancestral gene order, mainly involving in the four tRNA clusters (E-C-Y-Q-I-A, D-K, N-F-S1-R, and M-V). The nucleotide sequences of 13 protein-coding genes of this sequence and another seven species from Platygastridae were used for phylogenetic analysis by MrBayes, with two species from Cynipoidea as an outgroup. The topology demonstrated that T. remus was most closely related to Telenomus sp.