Antiviral memory B cells exhibit enhanced innate immune response facilitated by epigenetic memory.

The long-lasting humoral immunity induced by viral infections or vaccinations depends on memory B cells with greatly increased affinity to viral antigens, which are evolved from germinal center (GC) responses. However, it is unclear whether antiviral memory B cells represent a distinct subset among the highly heterogeneous memory B cell population. Here, we examined memory B cells induced by a virus-mimicking antigen at both transcriptome and epigenetic levels and found unexpectedly that antiviral memory B cells exhibit an enhanced innate immune response, which appeared to be facilitated by the epigenetic memory that is established through the memory B cell development. In addition, T-bet is associated with the altered chromatin architecture and is required for the formation of the antiviral memory B cells. Thus, antiviral memory B cells are distinct from other GC-derived memory B cells in both physiological functions and epigenetic landmarks.

Temperature-insensitive optical fiber strain sensor fabricated by two parallel connection Fabry-Perot interferometers with air-bubbles.

A strain sensor formed by a parallel connection of two Fabry-Perot interferometers (FPI) is proposed. The femtosecond laser is used to process a micro groove on the end face of a single-mode fiber (SMF), and then, it is welded with another SMF to form a small air bubble at the fusion point, fabricating an FPI. When the axial strain acts on the air bubble, the transverse length of the air bubble will change, causing the air cavity of the FPI to be easily deformed, and FPI can obtain high strain sensitivity. Three FPIs were manufactured with the air bubble sizes of 63, 78, and 93 µm, respectively, and the strain sensitivities of the three FPIs are 2.9, 2.0, and 1.5 pm/µÎµ, respectively. The experimental results show that the smaller the air bubble, the higher the strain sensitivity of FPI. Since the free spectral ranges of the three FPIs are relatively similar, we, respectively, paralleled them to form two Vernier effect strain sensors, and their sensitivities are -14.9 and -14.5 pm/µÎµ, respectively. Their sensitivities are increased by 5.1 times and 7.3 times, respectively. In addition, because three FPIs are composed of air cavities, they have very low temperature sensitivities. When they are connected in parallel, their resonance peak wavelength moves in the same direction with an increase in temperature, forming a reduced Vernier effect, and the temperature sensitivity amplification is very small. Therefore, the temperature cross-sensitivity of the sensor is extremely low and can be ignored.

Exploring the photovoltaic properties of promising non-fullerene acceptors with different degrees of asymmetry due to halogenations of terminal groups.

Over the past few years, the strategy of asymmetric modification has become popular for designing new photovoltaic materials because it can effectively improve optoelectronic performance and morphology, therefore power conversion efficiency (PCE). However, how the halogenations (to further change asymmetry) of terminal groups (TGs) of an asymmetric small-molecule non-fullerene acceptor (Asy-SM-NFA) influence optoelectronic properties is still not very clear. In this work, we have selected a promising Asy-SM-NFA IDTBF (the OSC based on it has a PCE of 10.43 %), exacerbated the asymmetry through fluorinations of TGs, and finally designed six new molecules. Based on density functional theory (DFT) and time-dependent DFT calculations, we systematically examine how the changed asymmetry impacts the optoelectronic properties. We find that the halogenations of TGs may significantly affect the molecular planarity, dipole moment, electrostatic potential, exciton binding energy, energy loss, and absorption spectrum. And the results show that newly designed BR-F1 and IM-mF (m = 1,3, and 4) are potential Asy-SM-NFAs because they all have enhanced absorption spectra in the visible region. Therefore, we provide a meaningful direction for the design of asymmetric NFA.

Compact highly sensitive Fabry-Perot temperature and gas pressure sensing probe fabricated by a femtosecond laser and PDMS.

A high sensitivity optical fiber temperature and gas pressure sensor with integrated micro-cavity is proposed. First, a single-mode optical fiber (SMF) is spliced with a section of capillary, and then the sensitive material polydimethylsiloxane (PDMS) is filled into the capillary to form a Fabry-Perot interferometer (FPI). Finally, a femtosecond laser is used to ablate the fiber core of the SMF to form the third reflecting surface, constituting two cascaded FPIs. When two FPIs have a similar free spectral range, a Vernier effect is produced. The temperature and gas pressure sensitivity of the sensor reached 14.41 nm/°C and 113.82 nm/MPa, respectively, after using the sensitive material and Vernier effect double sensitization technology. In addition, a fiber Bragg grating is cascaded with the sensor, which can realize the simultaneous measurement of temperature and gas pressure and eliminate cross-sensitivity.

High sensitivity temperature sensor based on enhanced Vernier effect through two parallel Fabry-Perot cavities.

In this paper, an enhanced Vernier effect temperature sensor based on two parallel Fabry-Perot interferometers (FPIs) is proposed and demonstrated experimentally. Among them, F P I 1 is composed of a single-mode fiber (SMF), a quartz capillary, and AB glue filled in the capillary. F P I 2 is formed by filling a capillary with polyimide (PI) solution and inserting two-segment SMF from both sides of the capillary. Since AB glue and PI have good thermal sensitivity, F P I 1 and F P I 2 are highly sensitive to temperature. Due to their different structures, the temperature sensitivity of F P I 1 is negative, and that of F P I 2 is positive. When F P I 1 and F P I 2 with similar free spectral range are connected in parallel, they will act as reference cavities for each other, resulting in an enhanced Vernier effect, which enlarges the sensitivity of the sensor more. In the temperature range of 40°C-58°C, the temperature sensitivity of the sensor is as high as -13.09n m/∘ C, and the fitting coefficient is 0.9974. The experimental results show that in the enhanced Vernier effect sensor structure, only two FPIs with opposite temperature sensitivity are required, which does not increase the difficulty and cost of sensor manufacturing. In addition, the sensor has good stability and repeatability.

Effect of seabuckthorn seed protein and its arginine-enriched peptides on combating memory impairment in mice.

The current study characterized the combating memory impairment effect of seabuckthorn seed protein (SSP) and the arginine (Arg)-enriched peptides (SSPP) on d-galactose-induced brain aging in mice. The Arg content in SSP and SSPP were 10.11 and 17.82 g/100 g, respectively. Seven Arg peptides (Ile/Leu-Arg, Arg-Glu, Asp-Arg-Pro, Arg-Try-Ala, Glu-Arg-Ser, Val-Gly-Arg-Pro, and Lys-Thr-Glu-Arg) were identified from SSPP. The animal experiments of the Morris water maze and the step-down test indicated that the oral administration of SSP (0.25, 0.5, 1.0 mg/g·d) and SSPP (0.25, 0.5, 1.0 mg/g·d) significantly (p < 0.05) reversed the learning and memory impairment symptoms. The activation of endothelial nitric oxide (NO) synthase and neuronal NO synthase were increased, and inducible NO synthase decreased after SSP and SSPP in the hippocampus compared to the model group, with the SSPP being quite effective. Moreover, the treatment significantly exhibited the ability to normalize the serum inflammatory cytokine levels (NF-ĸB, TNF-α, IL-6) and suppress the Arg-inducible nitric oxide (Arg-iNO) pathway. Therefore, SSP and SSPP ingestion reversed the behavioral learning and memory impairment symptoms possibly associated with the anti-inflammation and Arg-iNO pathway. Consumption of SSP and SSPP diets can be beneficial to memory impairment.

Basic electrolyzed water coupled with ultrasonic treatment improves the functional properties and digestibility of Antarctic krill proteins.

This study firstly constructed a new method of basic electrolyzed water coupled with ultrasonic (USBEW) to creatively modify the Antarctic krill proteins (AKPs), and its effects on amino acid composition, structural and functional properties, as well as in vitro digestibility of AKPs were evaluated. Results showed that BEW inhibited the production of 1O2 and •OH radicals from ultrasonic treatment. In comparison with the deionized water coupled with ultrasonic (USDW) treatment, the USBEW treatment effectively reduced the oxidation of active groups (carbonyl and free sulfhydryl) in the side chain of amino acids of the AKPs, and it also obviously decreased the particle size (18.6 nm), improved the solubility (13.2 %), increased the molecules flexibility and the surface hydrophobicity of AKPs. All these advantageous changes contributed to the improved foam stability, foam capacity and emulsifying properties of AKPs. More importantly, the in vitro digestibility (84.6 %) of AKPs treated by USBEW was significantly higher than that any control samples. Our results prove that the USBEW treatment is a valid and promising method to exploit novel marine protein products with a high nutritional quality.

The anti-anxiety/depression effect of a combined complex of casein hydrolysate and γ-aminobutyric acid on C57BL/6 mice.

In view of a series of adverse side effects of drugs for anxiety/depression on the market at present, it is imminent to extract and develop novel anti-anxiety and depression drugs from plants and proteins (like casein hydrolysate) as adjuncts or substitutes for existing anti-anxiety and depression drugs. Consequently, this study investigated the improvement of the anxiety/depression function by the compound of casein hydrolysate and γ-aminobutyric acid (GABA) (casein hydrolysate: GABA = 4:1; CCHAA) on mice induced by chronic restraint stress-corticosterone injection. Animal experiments revealed that oral gavage administration of CCHAA significantly reversed the anxiety/depression-like behaviors. Compared to the model control group, body weights were increased after treatment with CCHAA groups [1.5, 0.75 mg/(g⋅d)]. As a diagnostic index of anxiety and depression, we assessed GABA and 5-HT levels in response to CCHAA ingestion. The GABA and 5-HT levels were increasingly enhanced by the CCHAA diet. In addition, histopathological changes in the hippocampus CA3 region of the anxious/depressed mice were also alleviated after the treatment with the CCHAA. Thus, the casein hydrolysate and GABA formula diets may induce beneficial effects on the mice with anxiety/depression.

Highly sensitive gas pressure sensor based on the enhanced Vernier effect through a cascaded Fabry-Perot and Mach-Zehnder interferometer.

A high sensitivity optical fiber gas pressure sensor based on the enhanced Vernier effect is proposed. The sensor is composed of a fiber Fabry-Perot interferometer (FPI) and Mach-Zehnder interferometer (MZI). Since the interference fringes of FPI and MZI drift in the opposite direction with the change of gas pressure, when their free spectral ranges are similar, the enhanced Vernier effect is formed after their cascading. Compared with the traditional Vernier effect gas pressure sensor, the enhanced Vernier effect gas pressure sensor realizes much higher sensitivity gas pressure measurement without complex manufacturing process or desensitized reference interferometer. The experimental results show that the sensitivity of the enhanced Vernier effect sensor is 241.87 nm/MPa. In the two traditional Vernier effect gas pressure sensors formed by cascading FPI and MZI, the sensitivity of sensor is 63.02 nm/MPa and 171.26 nm/MPa, respectively. Compared with the two traditional Vernier effect sensors, the sensitivity of the enhanced Vernier effect sensor is increased by 3.8 times and 1.4 times, respectively. The proposed sensor also has the advantages of good repeatability and stability, fast response, low cost and easy manufacture. Our structure also provides a new design scheme for a high sensitivity optical fiber gas pressure sensor.

High sensitivity gas pressure sensor based on different inner diameter quartz capillary cascading and Vernier effect.

In this paper, a highly sensitive optical fiber gas pressure sensor is proposed and experimentally verified. The sensor is composed of two Fabry-Pérot (F-P) cavities, and two F-P cavities are fabricated by a single-mode fiber and two quartz capillaries with different inner diameters splicing. Among them, the small inner diameter capillary is used as a gas channel connecting the large inner diameter capillary and the external environment. The manufacturing process of the sensor only involves capillary cleaver and splicing and does not involve other complex manufacturing technologies. By correctly adjusting the length of the two quartz capillaries, when the free spectral range of the two F-P cavities is very close, the optical Vernier effect will be observed and used as a sensitive probe for detecting gas pressure. The experimental results show that, in the pressure range of 0-0.8 MPa, the gas pressure sensitivity of the sensor reaches -81.73 nm/MPa with a linearity of 99.7%, and the temperature cross-sensitivity is only 1.82 kPa/°C. Due to its easy manufacture, high sensitivity, compact structure, and small volume, the sensor has become one of the preferred structures for large-scale use in the field of gas sensing.

Development and validation of a novel nomogram for predicting systemic inflammatory response syndrome's occurrence in patients undertaking flexible ureteroscopy.

Background: The occurrence of systemic inflammatory response syndrome (SIRS) is an early alert for sepsis after flexible ureteroscopy (fURS). Once sepsis occurs, it often leads to severe or fatal consequences. We aimed to identify SIRS patients preoperatively by developing and validating a feasible prognostic nomogram model based on retrospective cohort analysis. Methods: A total of 311 patients who underwent fURS in Dongguan Kanghua Hospital (Dongguan, China) between 2016 and 2020 were included and randomly divided into a primary cohort (n=219) and validation cohort (n=92). Single factor regression analysis was used to identify the primary cohort's meaningful characters between SIRS and non-SIRS groups. Factors of the primary cohort were then identified by least absolute shrinkage and selection operator (LASSO) regression analysis, and a nomogram was built to execute the subsequent analysis using these factors. Finally, we analyzed and drew the calibration curve, receiver operating characteristic (ROC) curve, and decision curve analysis (DCA) curve to validate the prognostic value of the nomogram in calibration and discrimination. Results: Review of the single regression analysis of characters in the primary cohort showed gender, stone burden, diabetes, neutrophil (N), lymphocyte (L), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), lymphocyte-to-monocytes ratio (LMR), urine-WBC, nitrite (Nit), urine culture, and surgery time as significant factors between the SIRS and non-SIRS groups (P<0.05). The LASSO regression analysis suggested NLR, PLR, and urine culture were substantial factors in predicting SIRS postoperatively, lambda.min and lambda.1se (standard error, SE) were 0.01491 and 0.0796. A nomogram built with the three factors showed good calibration and discrimination, with the Brier values 0.064 and 0.034 and the area under curve (AUC) values 0.897 (95% CI: 0.837-0.957) and 0.976 (95% CI: 0.947-1.000) in the primary and validation cohort, respectively. DCA demonstrated the nomogram was clinically useful, and the predict probability of SIRS's occurrence was very close to the actual rate as the risk threshold increased by higher than 60% in clinical impact curve analysis. Conclusions: NLR, PLR, and urine culture were significantly related to the occurrence of SIRS's after fURS. The nomogram with these three factors showed excellent calibration, discrimination, and clinical usefulness.

Preparation of ß-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish.

This study aimed to obtain three Trp-containing peptides from ß-lactoglobulin and study their effects on anxiety-like behaviors in zebrafish. Three Trp-containing peptides were prepared from ß-lactoglobulin by selective enzymatic hydrolysis and identified by UPLC-Q-TOF MS/MS. The anxiety-like behaviors of zebrafish were reduced after two weeks of administrated of ß-lactoglobulin Trp peptides (LAWP), VAGTWY, VAGTW and G TW(concentration of 56 µg/mL or 500 µg/mL). As an index of serotonergic activity, we assessed the enhancing abilities of 5-HT synthesis. The treatment remarkably enhanced the 5-HT synthesis by upregulation of Trp concentration and Trp hydroxylase activation. In addition, this study further validated the anti-anxiety effects of whey protein hydrolysate with a high Trp index in animal and the experimental results were consistent with those reported in previous studies. Our results showed that ß-lactoglobulin Trp peptides ingestion has a significant anti-anxiety effect as evidenced by the increasing Trp concentration, TPH activation and 5-HT level compared to the control group, with the VAGTW being the more effective.

Hypoglycemic Effect of Hydrophobic BCAA Peptides Is Associated with Altered PI3K/Akt Protein Expression.

The hypoglycemic activities of the hydrophobic branched-chain amino acid (BCAA) peptides from seabuckthorn seed protein were preliminarily characterized in type 2 diabetic db/db mice. Four novel BCAA peptides (18.27 ± 0.26% (w/w): Leu/Ile-Pro-Glu-Asp-Pro, Asp-Leu/Ile-Val-Gly-Glu, Leu/Ile-Pro, and Leu/Ile-Pro-Leu/Ile) were identified in seabuckthorn seed protein. The protein content in seabuckthorn seed protein hydrolysate, obtained using 80% ethanol, was 78.8 ± 1.4% (w/w). Animal experiments revealed that oral administration of BCAA peptides (all four) significantly reversed the diabetic symptoms. Compared to the db/db group (control), body weight and insulin resistance were ameliorated after treatment with BCAA peptides (0.5, 1.0, 2.0 mg/(g d)). Also, the treatment remarkably reduced the fasting blood glucose (FBG) levels by upregulation of glucose transporter 4 (GULT4). Moreover, BCAA peptides significantly increased the muscle glycogen content (22.6 ± 0.9 nmol/mg) via the downregulation of protein kinase B (AKT) and glycogen synthase kinase-3ß (GSK-3ß) while increasing the activity of glycogen synthase (GS). BCAA peptides also significantly upregulated the protein levels of phosphatidylinositol 3-kinase (PI3K). We show that BCAA peptides alleviated insulin resistance associated with altered PI3K/Akt protein expression in the skeletal muscle of db/db mice.

Comparative study on the novel umami-active peptides of the whole soybeans and the defatted soybeans fermented soy sauce.

BACKGROUND: Two kinds of soy sauce produced via fermentation of the whole soybeans and the defatted soybeans (soy sauce termed 'SSS' and 'SSD', respectively) were subjected to the treatment using aqueous ethanol solutions with high concentrations. Then tasty peptides were separated from SSS and SSD by sensory guided fractionation, using macroporous resin and reverse-phase high-performance liquid chromatography (RP-HPLC), and identified by ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). RESULTS: The result showed that umami-active fractions and ≤ 3 kDa peptides were mainly concentrated in the supernatants resulted from the treatment with 60% ethanol and the precipitates resulted from the treatment with 80% ethanol. The contents of ammonia nitrogen, non-salt solids, bitter amino acids, amino acids in peptides and the proportion of ≤ 3 kDa peptides in SSS were higher than those in SSD. Sixteen and fourteen tasty peptides were separated from SSS and SSD, among them five dipeptides (γ-Glu-Glu, Glu-Glu, γ-Glu-Cys, γ-Glu-Leu, Glu-Leu and Ile-Glu) with a glutamic acid residue were identified both in SSS and SSD, which have been reported as umami/kokumi-active peptides in soy sauce. Several peptides identified from SSS (Thr-Gly-Cys, Gly-Leu-Glu, Val-Glu-Ala-Leu and Gly-Gly-Gly-Glu) and SSD (Asp-Arg, Asp-Ala-Glu, Glu-Val-Cys and Gly-Gly-Gly-Glu) are tasty and/or umami-active peptides but have not been reported as tasty peptides in the past. CONCLUSION: Most of the peptides separated from SSD and SSS could impart an umami-enhancing effect on soy sauce, and the marginally more kokumi and bitterness hydrophobic peptides were found in SSS than SSD. © 2020 Society of Chemical Industry.

The effects of Sea buckthorn seed protein on glucose metabolism in streptozotocin-induced diabetic ICR mice.

In this paper, we investigated the effects of protein from sea buckthorn seed on the expression of genes involved in liver glucose metabolism and on the activation of the AMPK/SIRT1 pathway in streptozotocin (STZ)-induced diabetic ICR mice. The investigated factors included oral glucose tolerance test, insulin resistance, insulin sensitivity index, AMPK and SIRT1 activity and the expression of liver glucose metabolism genes. Seabuckthorn seed protein (SSP) improved the oral glucose tolerance and insulin sensitivity, reduced insulin resistance, suppressed expression of liver glucose metabolism genes and upregulated activation of the AMPK/SIRT1 pathway. Therefore, the results demonstrate that SSP can improve insulin resistance, suppress expression of these genes and upregulate activation on the AMPK /SIRT1 pathway in STZ-induced diabetic ICR mice.

γ-[Glu]n-Trp ameliorates anxiety/depression-like behaviors and its anti-inflammatory effect in an animal model of anxiety/depression.

This study compared the abilities of γ-[Glu]n-Trp (EW) and whey protein hydrolysate (WPH) with a high ratio of Trp : 5 large neutral amino acids (5LNAAs) to reverse chronic restraint stress-corticosterone injection induced anxiety/depression-like behaviors in C57BL/6 male mice. EW was synthesized using l-glutaminase from Bacillus amyloliquefaciens. Acid protease, trypsin, pancreatin or flavorzyme was used to produce WPHs. The WPH with the highest Trp/5LNAAs ratio (17.38%; by trypsin) was selected for animal trials. EW (dose 2.0, 5.0 or 10.0 mg kg-1 d-1) and WPH (dose 0.5, 1.0 or 2.0 mg g-1 d-1) reversed behavioral dysfunctions, suppressed serum inflammatory cytokines (TNF-α, IL-6, IL-1ß and IFN-γ), and reduced the activity of indoleamine 2,3-dioxygenase (key rate-limiting enzyme of the kynurenine pathway) while increasing the activity of tryptophan hydroxylase (key rate-limiting enzyme of the serotonin pathway) in the hypothalamus, hippocampus and prefrontal cortex, with EW acting more effectively. EW could also increase body weight gain and might act more effectively via the kynurenine pathway. These findings are of significance to promote the future practical application of kokumi γ-[Glu]n-Trp peptides.

B Cells Are the Dominant Antigen-Presenting Cells that Activate Naive CD4+ T Cells upon Immunization with a Virus-Derived Nanoparticle Antigen.

B cells can present antigens to CD4+ T cells, but it is thought that dendritic cells (DCs) are the primary initiators of naive CD4+ T cell responses. Nanoparticles, including virus-like particles (VLPs), are attractive candidates as carriers for vaccines and drug delivery. Using RNA phage Qß-derived VLP (Qß-VLP) as a model antigen, we found that antigen-specific B cells were the dominant antigen-presenting cells that initiated naive CD4+ T cell activation. B cells were sufficient to induce T follicular helper cell development in the absence of DCs. Qß-specific B cells promoted CD4+ T cell proliferation and differentiation via cognate interactions and through Toll-like receptor signaling-mediated cytokine production. Antigen-specific B cells were also involved in initiating CD4+ T cell responses during immunization with inactivated influenza virus. These findings have implications for the rational design of nanoparticles as vaccine candidates, particularly for therapeutic vaccines that aim to break immune tolerance.

Effects of dual modified resistant indica rice starch on azoxymethane-induced incipient colon cancer in mice.

In this study, the effects of different doses of dual modification-treated (DMT) indica rice resistant starch (IR-RS) on azoxymethane (AOM)-induced early colon cancer in mice were investigated. The investigated factors included body weight, gastrointestinal emptying rate, the number and morphology of aberrant crypt foci (ACFs) and the specific expressions of adenomatous polyposis coli (APC), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax) and cytochrome c genes. The results demonstrated that DMT IR-RS controlled the increase in the body weights of the mice, increased the gastrointestinal emptying rates and reduced the numbers of ACFs and aberrant crypts. Reverse transcription-polymerase chain reaction revealed that DMT IR-RS promoted the expression of APC, Bax and cytochrome c and inhibited the expression of Bcl-2. These results demonstrate that a DMT IR-RS diet may induce apoptosis and has beneficial health effects in AOM-induced early colon cancer in mice.

Effects of a treatment with Se-rich rice flour high in resistant starch on enteric dysbiosis and chronic inflammation in diabetic ICR mice.

BACKGROUND: Enteric dysbiosis is associated with chronic inflammation and interacts with obesity and insulin resistance. Obesity and diabetes are induced in ICR (Institute of Cancer Research) mice fed a high-fat diet and administered a streptozocin injection. These mice were treated with normal rice (NR), normal rice with a high resistant starch content (NRRS) or Se-rich rice (selenium-enriched rice) with a high resistant starch content (SRRS). RESULTS: Faecal cell counts of Bifidobacterium, Lactobacillus and Enterococcus were significantly higher in SRRS-treated mice than in diabetic controls, while Enterobacter cloacae were lower. Similar results were also found in NRRS-treated mice. In contrast, no significant difference was found between NR-treated and diabetic control groups. The treatments with SRRS and NRRS reduced the faecal pH values of the diabetic mice. Regarding the inflammatory factor levels, lower levels of serum C-reactive protein (CRP), tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), nuclear factor-k-gene binding (NF-κB) and leptin (LEP) and higher adiponutrin (ADPN) levels were found in the SRRS and NRRS-treated mice compared with the diabetic and NR-treated mice. In addition, the CRP, IL-6 and NF-κB levels in the SRRS-treated mice were significantly reduced compared with those observed in the NRRS-treated mice. The reverse transcription-PCR (RT-PCR) results showed that the SRRS and NRRS-treated mice presented higher expression levels of orphan G protein-coupled receptor 41 (GPR41) and orphan G protein-coupled receptor 43 (GPR43) proteins compared with diabetic mice and NR-treated mice. CONCLUSION: These results indicate that treatments with rice high in RS exert beneficial effects by improving enteric dysbiosis and chronic inflammation. In addition, selenium and RS may exert synergistic effects on chronic inflammation. © 2016 Society of Chemical Industry.

[The evaluation of curative effect of radiofrequency thermocoagulation on semilunar ganglion of aged patients with recurrent trigeminal neuralgia].

OBJECTIVE: To investigate curative effect and importance of radiofrequency thermocoagulation onsemilunar ganglion of aged patients with recurrent trigeminal neuralgia. METHOD: 112 cases with recurrent trigeminal neuralgia were performed with radiofrequency thermocoagulation on semilunar ganglion by puncture through skin under local anesthesia. RESULT: The maintaining effective duration in 112 cases was 7.3 ± 1.3 years after primary therapy. After the first thermocoagulation, 89.28% (100/112) patients got the relief from pain immediately, 8 (7.14%) cases also recieved the same effect after twice treatment. However two cases (2.6%) had no effects. The total effective rate was 98.21%. During five follow-up years, VAS scores, dose of carbamazepine and satisfaction were better than those before the treatment. The effective rate was still over 57.5% within three years after the treatment. The recurrent risk increased obviously from 40 months after the treatment by Kaplan-Meier method. CONCLUSION: Semilunar ganglion of radiofrequency thermocoagulation was an ideal option for old-age patients with trigeminal neuralgia. It can be performed repeatedly.