Decreased ATF5 level contributes to improved mitochondrial function in oocytes exposed to vitrification stress.

Background: Mitochondrial unfolded protein response (mtUPR) plays an essential role in the response of mitochondria to stress-induced damage. Activating of transcription factor 5 (ATF5) can help to sustain mitochondrial function and regulate organelle recovery under mitochondrial stress. Vitrification is a stressor that disrupts mitochondrial activity and cell homeostasis. However, little is known about the function of ATF5 in response to the extreme biophysical and chemical stresses during oocyte vitrification. Methods: The expression of ATF5 and mtUPR biomarkers were measured in fresh and vitrified oocytes. Subsequently, oocytes with ATF5 deficiency were constructed by siRNA microinjection, and the function of ATF5 in mitochondrial function and oocyte development were analyzed in vitrified oocytes. Furthermore, transcriptome analysis was performed to uncover the molecular network regulated by ATF5 in response to oocyte vitrification. Results: In the present study, the mitochondrial membrane potential and ATP levels were decreased in ATF5 knockdown oocytes, in line with the phenotypes observed in vitrified oocytes. In addition, ATF5 knockdown resulted in decreased mitochondrial temperature, reduced unfolded protein levels, abnormal mitochondrial dynamics (fusion and fission), and increased autophagy. Subsequent experiments indicated that mtUPR was suppressed in oocytes with ATF5 knockdown. Interestingly, ATF5 was aberrantly upregulated in oocytes exposed to vitrification stress. Reduced ATF5 expression to a homeostatic level in vitrified oocytes led to accumulated unfolded protein levels and increased mitochondrial membrane potential. Moreover, increased mitochondrial dynamics and an increased germinal vesicle breakdown (GVBD) rate were detected after in vitro maturation. Transcriptome analysis revealed that ATF5 is involved in the vitrification stress response, and ATF5 regulated the in vitro maturation potential in vitrified oocytes through the cAMP-PKA and PI3K/AKT pathways. Discussion: Our findings indicate that mtUPR was initiated in response to vitrification stimuli, and downregulated ATF5 level to a homeostatic state contributes to improved mitochondrial function in oocytes exposed to vitrification stress. Our results highlight the crucial role of ATF5 in the regulation of mitochondrial function in vitrified oocytes through mediating mtUPR.

A metabolomic profile of biological aging in 250,341 individuals from the UK Biobank.

The metabolomic profile of aging is complex. Here, we analyse 325 nuclear magnetic resonance (NMR) biomarkers from 250,341 UK Biobank participants, identifying 54 representative aging-related biomarkers associated with all-cause mortality. We conduct genome-wide association studies (GWAS) for these 325 biomarkers using whole-genome sequencing (WGS) data from 95,372 individuals and perform multivariable Mendelian randomization (MVMR) analyses, discovering 439 candidate "biomarker - disease" causal pairs at the nominal significance level. We develop a metabolomic aging score that outperforms other aging metrics in predicting short-term mortality risk and exhibits strong potential for discriminating aging-accelerated populations and improving disease risk prediction. A longitudinal analysis of 13,263 individuals enables us to calculate a metabolomic aging rate which provides more refined aging assessments and to identify candidate anti-aging and pro-aging NMR biomarkers. Taken together, our study has presented a comprehensive aging-related metabolomic profile and highlighted its potential for personalized aging monitoring and early disease intervention.

Optical continuous pulse position modulation-based analog RoF via frequency-domain position estimation.

Analog radio-over-fiber (A-RoF) solutions for mobile fronthaul are regaining wide attention due to their high spectral efficiency and low complexity. However, the performance of A-RoF is usually limited by the fiber link fidelity. In this Letter, we propose and experimentally demonstrate an optical continuous pulse position modulation-based analog radio-over-fiber (OCPPM-RoF) scheme, in which the amplitudes of wireless waveforms are mapped to the time-domain positions of optical pulses to decouple the additive noise. The de-modulation of OCPPM-RoF signals is performed by a frequency-domain continuous position estimation (FD-CPE) algorithm including cross-power spectrum calculation and weighted least square for accurate delay estimation. In the experiment, by adopting appropriate pulse width factors, the recovered signal-to-noise ratio (SNR) can be flexibly adjusted within a wide range from 31.8 to 54.0 dB, supporting the transmission of various formats from 256-QAM to 65536-QAM. The results indicate that OCPPM-RoF can be a promising candidate for future fronthaul.

High-fidelity sub-petabit-per-second self-homodyne fronthaul using broadband electro-optic combs.

With the exponential growth in data density and user ends of wireless networks, fronthaul is tasked with supporting aggregate bandwidths exceeding thousands of gigahertz while accommodating high-order modulation formats. However, it must address the bandwidth and noise limitations imposed by optical links and devices in a cost-efficient manner. Here we demonstrate a high-fidelity fronthaul system enabled by self-homodyne digital-analog radio-over-fiber superchannels, using a broadband electro-optic comb and uncoupled multicore fiber. This self-homodyne superchannel architecture not only offers capacity boosting but also supports carrier-recovery-free reception. Our approach achieves a record-breaking 15,000 GHz aggregated wireless bandwidth, corresponding to a 0.879 Pb/s common public radio interface (CPRI) equivalent data rate. Higher-order formats up to 1,048,576 quadrature-amplitude-modulated (QAM) are showcased at a 100 Tb/s class data rate. Furthermore, we employ a packaged on-chip electro-optic comb as the sole optical source to reduce the cost, supporting a data rate of 100.5 Tb/s with the 1024-QAM format. These demonstrations propel fronthaul into the era of Pb/s-level capacity and exhibit the promising potential of integrated-photonics implementation, pushing the boundaries to new heights in terms of capacity, fidelity, and cost.

Overcoming laser phase noise for low-cost coherent optical communication.

Artificial-intelligence-generated content has driven explosive data traffic growth in data-center interconnects. Traditional direct detection solutions struggle with limited spectral efficiency and distance, prompting the shift to coherent optics for cost-sensitive short-reach links. One specific challenge is integrating low-cost lasers while overcoming severe phase noise on high-order modulation formats. Here, we propose a residual carrier modulation scheme for precise and efficient carrier frequency and phase recovery. The residual optical carrier can continuously track phase fluctuations without redundancy compared with discrete time-domain pilots, and address the digital-to-analog convertor resolution reduction issue of frequency-domain digital pilots. In proof-of-concept experiments, we transmit a net 1-Tb/s probabilistic-shaped 256-ary quadrature amplitude modulated (PS-256-QAM) signal using a 3 MHz distributed feedback (DFB) laser. Our scheme improves bitrate by 41% compared to conventional time-domain pilots, achieving a record laser linewidth sum and symbol duration product of 6.89 × 10-5. This approach supports MHz linewidth DFB lasers in low-cost coherent optical communications.

The hidden threat: Comprehensive assessment of antibiotic and disinfectant resistance in commercial pig slaughterhouses.

The presence of antibiotic resistance genes (ARGs), disinfectant resistance genes (DRGs), and pathogens in animal food processing environments (FAPE) poses a significant risk to human health. However, knowledge of the contamination and risk profiles of a typical commercial pig slaughterhouse with periodic disinfectant applications is limited. By creating the overall metagenomics-based behavior and risk profiles of ARGs, DRGs, and microbiomes in a nine-section pig slaughterhouse, an important FAPE in China. A total of 454 ARGs and 84 DRGs were detected in the slaughterhouse with resistance genes for aminoglycosides and quaternary ammonium compounds, respectively. The entire slaughtering chain is a hotspot for pathogens, including 83 human pathogenic bacteria (HPB), with 47 core HPB. In addition, 68 high-risk ARGs were significantly correlated with 55 HPB, 30 of which were recognized as potential bacteria co-resistant to antibiotics and disinfectants, confirm a three-fold risk of ARGs, DRGs, and pathogens prevailing throughout the chain. Pre-slaughter pig house (PSPH) was the major risk source for ARGs, DRGs, and HPB. Moreover, 75 Escherichia coli and 47 Proteus mirabilis isolates showed sensitivity to potassium monopersulfate and sodium hypochlorite, suggesting that slaughterhouses should use such related disinfectants. By using whole genome multi-locus sequence typing and single nucleotide polymorphism analyses, genetically closely related bacteria were identified across distinct slaughter sections, suggesting bacterial transmission across the slaughter chain. Overall, this study underscores the critical role of the PSPH section as a major source of HPB, ARGs, and DRGs contamination in commercial pig slaughterhouses. Moreover, it highlights the importance of addressing clonal transmission and cross-contamination of antibiotic- and disinfectant-resistant bacteria within and between slaughter sections. These issues are primarily attributed to the microbial load carried by animals before slaughter, carcass handling, and content exposure during visceral treatment. Our findings provide valuable insights for One Health-oriented slaughterhouse management practices.

Procyanidin B2 improves developmental capacity of bovine oocytes via promoting PPARγ/UCP1-mediated uncoupling lipid catabolism during in vitro maturation.

Metabolic balance is essential for oocyte maturation and acquisition of developmental capacity. Suboptimal conditions of in vitro cultures would lead to lipid accumulation and finally result in disrupted oocyte metabolism. However, the effect and mechanism underlying lipid catabolism in oocyte development remain elusive currently. In the present study, we observed enhanced developmental capacity in Procyanidin B2 (PCB2) treated oocytes during in vitro maturation. Meanwhile, reduced oxidative stress and declined apoptosis were found in oocytes after PCB2 treatment. Further studies confirmed that oocytes treated with PCB2 preferred to lipids catabolism, leading to a notable decrease in lipid accumulation. Subsequent analyses revealed that mitochondrial uncoupling was involved in lipid catabolism, and suppression of uncoupling protein 1 (UCP1) would abrogate the elevated lipid consumption mediated by PCB2. Notably, we identified peroxisome proliferator-activated receptor gamma (PPARγ) as a potential target of PCB2 by docking analysis. Subsequent mechanistic studies revealed that PCB2 improved oocyte development capacity and attenuated oxidative stress by activating PPARγ mediated mitochondrial uncoupling. Our findings identify that PCB2 intricately improves oocyte development capacity through targeted activation of the PPARγ/UCP1 pathway, fostering uncoupling lipid catabolism while concurrently mitigating oxidative stress.

Broadband ASE source-enabled self-homodyne DA-RoF fronthaul using cascaded SOAs and a multicore fiber.

Broadband amplified spontaneous emission (ASE) light sources are recognized for their cost-effective generation. However, their inherent high-intensity noise and the stringent requirement for time delay matching limits their widespread application in coherent optical telecommunication. Here we propose a broadband ASE source-enabled digital-analog radio-over-fiber (DA-RoF) mobile fronthaul architecture, leveraging semiconductor optical amplifiers (SOAs) and multicore fiber in tandem. Our proposed system uses SOAs to suppress the intensity noise of the ASE carrier and transmits the DA-RoF signal alongside an unmodulated carrier through distinct cores of an 8-core, 1-km fiber. This setup significantly enhances the signal-to-noise ratio (SNR) by 19.4 dB, boosts capacity, and enables self-homodyne detection at the receiver end. We achieve an aggregated bandwidth of 35 GHz (7 cores × 5 GHz), supporting a 2.05-Tb/s CPRI-equivalent data rate with 1024-ary quadrature-amplitude-modulated (1024-QAM) signals. Additionally, we analyze the impact of chromatic dispersion on signal-to-noise ratio for broadband source coherent detection systems. This innovative scheme offers a pragmatic solution for integrating low-cost broadband sources into cost-sensitive fronthaul systems, providing both high capacity and fidelity in massive deployment scenarios.

Gradient-descent noise whitening techniques for short-reach IM-DD optical interconnects with severe bandwidth limitation.

Bandwidth limitation in optoelectrical components and the chromatic dispersion-induced power fading phenomenon cause severe inter-symbol interference (ISI) in high-speed intensity modulation and direct detection (IM-DD) optical interconnects. While the equalizer implemented in the receiver's digital signal processing procedure can mitigate ISI, it also inevitably enhances the noise located in the decayed frequency region, known as equalization-enhanced colored noise (EECN). Additionally, the nonlinear impairments of the modulator and photodetector also deteriorate the performance of the IM-DD system, especially for high-order modulation formats. In this work, we propose a gradient-descent noise whitening (GD-NW) algorithm to address EECN and extend it by introducing nonlinear kernels to simultaneously mitigate EECN and nonlinear impairments. The proposed algorithms are compared with conventional counterparts in terms of the achievable baud rate and the receiver optical power sensitivity. As a proof-of-concept experiment, we validate the principles of the proposed algorithms by successfully transmitting 360-GBd on-off-keying (OOK) and 180-GBd 4-level pulse-amplitude-modulation (PAM-4) signals in the back-to-back case under a 62-GHz brick-wall bandwidth limitation. 280-GBd OOK and 150-GBd PAM-4 transmissions are also demonstrated over 1-km standard single-mode fiber with a bit error rate below 7% hard-decision forward error correction aided by the proposed approach.

Vitrification of Mammalian Oocytes: Recent Studies on Mitochondrial Dysfunction.

Vitrification of reproductive cells is definitely essential and integral in animal breeding, as well as in assisted reproduction. However, issues accompanied with this technology such as decreased oocyte competency and relatively low embryo survival rates appear to be a tough conundrum that has long perplexed us. As significant organelles in cell metabolism, mitochondria play pivotal roles in numerous pathways. Nonetheless, extensive evidence has demonstrated that vitrification can seriously impair mitochondrial function in mammalian oocytes. Thus, in this article, we summarize the current progress in oocyte vitrification and particularly outline the common mitochondrial abnormalities alongside subsequent injury cascades seen in mammalian oocytes following vitrification. Based on existing literature, we tentatively come up with the potential mechanisms related to mitochondrial dysfunction and generalize efficacious ways which have been recommended to restore mitochondrial function.

Antimicrobial resistance characteristics and phylogenetic relationships of pleuromutilin-resistant Enterococcus isolates from different environmental samples along a laying hen production chain.

Antimicrobial resistance in the laying hen production industry has become a serious public health problem. The antimicrobial resistance and phylogenetic relationships of the common conditional pathogen Enterococcus along the laying hen production chain have not been systematically clarified. 105 Enterococcus isolates were obtained from 115 environmental samples (air, dust, feces, flies, sewage, and soil) collected along the laying hen production chain (breeding chicken, chick, young chicken, and commercial laying hen). These Enterococcus isolates exhibited resistance to some clinically relevant antibiotics, such as tetracycline (92.4%), streptomycin (92.4%), and erythromycin (91.4%), and all strains had multidrug resistance phenotypes. Whole genome sequencing characterized 29 acquired antibiotic resistance genes (ARGs) that conferred resistance to 11 classes of antibiotics in 51 pleuromutilin-resistant Enterococcus isolates, and lsa(E), which mediates resistance to pleuromutilins, always co-occurred with lnu(B). Alignments with the Mobile Genetic Elements database identified four transposons (Tn554, Tn558, Tn6261, and Tn6674) with several ARGs (erm(A), ant(9)-la, fex(A), and optrA) that mediated resistance to many clinically important antibiotics. Moreover, we identified two new transposons that carried ARGs in the Tn554 family designated as Tn7508 and Tn7492. A complementary approach based on conventional multi-locus sequence typing and whole genome single nucleotide polymorphism analysis showed that phylogenetically related pleuromutilin-resistant Enterococcus isolates were widely distributed in various environments on different production farms. Our results indicate that environmental contamination by antimicrobial-resistant Enterococcus requires greater attention, and they highlight the risk of pleuromutilin-resistant Enterococcus and ARGs disseminating along the laying hen production chain, thereby warranting effective disinfection.

LncRNA MIR200CHG inhibits EMT in gastric cancer by stabilizing miR-200c from target-directed miRNA degradation.

Gastric cancer (GC) is a heterogeneous disease, threatening millions of lives worldwide, yet the functional roles of long non-coding RNAs (lncRNAs) in different GC subtypes remain poorly characterized. Microsatellite stable (MSS)/epithelial-mesenchymal transition (EMT) GC is the most aggressive subtype associated with a poor prognosis. Here, we apply integrated network analysis to uncover lncRNA heterogeneity between GC subtypes, and identify MIR200CHG as a master regulator mediating EMT specifically in MSS/EMT GC. The expression of MIR200CHG is silenced in MSS/EMT GC by promoter hypermethylation, associated with poor prognosis. MIR200CHG reverses the mesenchymal identity of GC cells in vitro and inhibits metastasis in vivo. Mechanistically, MIR200CHG not only facilitates the biogenesis of its intronic miRNAs miR-200c and miR-141, but also protects miR-200c from target-directed miRNA degradation (TDMD) through direct binding to miR-200c. Our studies reveal a landscape of a subtype-specific lncRNA regulatory network, providing clinically relevant biological insights towards MSS/EMT GC.

Computationally efficient composite triple beat cancellation for DML-based DA-RoF fronthaul.

The digital-analog radio-over-fiber (DA-RoF) scheme offers a high-fidelity and spectrally efficient solution for future mobile fronthaul. However, to be implemented in the low-cost directly modulated laser with direct detection (DML-DD) link, both the digital and analog parts in DA-RoF modulation would suffer from the composite second-order (CSO) and composite triple beat (CTB) caused by the chirp-dispersion interaction. In this Letter, we propose and experimentally demonstrate a computationally efficient composite triple beat cancellation (CTB-C) algorithm for DA-RoF fronthaul in the dispersion-uncompensated C-band DML-DD link. The CSO and CTB are suppressed at the receiver-side DSP based on the theoretical model of these nonlinear distortions. In the proof-of-concept experiment, a 1.2-dB improvement in the recovered signal-to-noise ratio (SNR) is obtained with 5.5-GHz 1024-QAM orthogonal frequency division multiplexing (OFDM) signal after 10-km standard single-mode fiber (SSMF) transmission. The proposed CTB-C technique does not require the training process and performs close to the Volterra-based feed-forward equalizer (VFE) under the complexity constraint.

Fast polarization state tracking for dual-polarization direct detection with Jones-space field recovery.

Direct detection system is expected to possess the phase and polarization diversity in order to achieve high spectral efficiency and fiber impairment compensation such as chromatic dispersion and polarization rotation. In this Letter, we theoretically extend the concept of the proposed Jones-space field recovery (JSFR) to include a dynamic polarization rotation matrix and experimentally demonstrate the rapid polarization state tracking ability of the JSFR receiver based on a 3 × 3 optical coupler. Under a rotation of the state of polarization at a rate of 1 Mrad/s, we successfully transmit 59-GBd dual-polarization 16-ary quadrature-amplitude-modulation signals over an 80-km standard single-mode fiber based on a decision-directed least mean square (DD-LMS) or a recursive least square (DD-RLS), with a bit-error rate below the 14% hard-decision forward error correction threshold of 1 × 10-2. The experimental results indicate that the legacy polarization tracking algorithms designed for coherent optical communication are also applicable for this direct detection scheme. To our best knowledge, this work demonstrates the first polarization rotation-tolerant direct detection system with phase and polarization diversity, providing a low-cost and high-speed solution for short-reach communications.

Digital pre-distortion using a Gauss-Newton-based direct learning architecture for coherent optical transmitters.

Digital pre-distortion (DPD) is a powerful technique to mitigate transmitter nonlinear distortion in optical transmissions. In this Letter, the identification of DPD coefficients based on the direct learning architecture (DLA) using the Gauss-Newton (GN) method is applied in optical communications for the first time. To the best of our knowledge, this is the first time that the DLA has been realized without training an auxiliary neural network to mitigate optical transmitter nonlinear distortion. We describe the principle of the DLA using the GN method and compare the DLA with the indirect learning architecture (ILA) that uses the least-square (LS) method. Extensive numerical and experimental results indicate that the GN-based DLA is superior to the LS-based ILA, especially in a low signal-to-noise ratio scenario.

Deciphering risks of resistomes and pathogens in intensive laying hen production chain.

Antimicrobial resistance (AMR) and pathogens derived from food animals and their associated environments have emerged as challenging threats to humans from a health perspective, but our understanding of these risks and their key prevention and control points in the current intensive breeding industry remains poor. By creating an integral composition and risk profile of the resistome and microbiome through metagenomics in feces, flies, dust, sewage, and soil along the four-stage laying hen production chain, we found that the whole production chain is a hotspot for antimicrobial resistance genes (ARGs) with 374 known subtypes and pathogens, including 157 human pathogenic bacteria (HPB). Feces and flies were identified as major risk sources for these contaminations. Also, we confirmed a twin-risk of AMR and pathogenicity prevailing throughout the chain, but with different frequencies in each stage; thus, high-risk ARGs in the young chicken stage and highly prioritized HPB in the chick stage contributed 37.33 % to the total AMR risk and 36.36 % to the pathogenic risks, respectively, thus rendering the two stages to be the key prevention points. Moreover, the prevalence of 112 binned ARG supercarriers (for example, Klebsiella pneumoniae harboring 20 ARGs) was unraveled along the production chain, especially in feces, flies, and dust, and 87 potential hosts exhibited high pathogenic risk, high-risk AMR, or both, with 262 ARGs and 816 virulence factor genes. Overall, this study provides first-hand comprehensive data on high-risk ARGs and their pathogenic hosts in the intensive laying hen production chain, and thus is fundamentally important for developing new measures to help control the global AMR crisis induced through the animal-environment-human pathway.

Coherent digital-analog radio-over-fiber (DA-RoF) system with a CPRI-equivalent data rate beyond 1 Tb/s for fronthaul.

We propose and experimentally demonstrate a coherent digital-analog radio-over-fiber (DA-RoF) system and achieve the transmission of Tb/s common public radio interface (CPRI)-equivalent data rate for fronthaul. The proposed coherent DA-RoF system includes DA-RoF modulation, demodulation and DA-RoF compatible coherent digital signal processing (DSP) blocks. A theoretical analysis of the DA-RoF scheme together with parameter optimization is accomplished as well. In the experiment, a 25 Gbaud DA-RoF signal with 1 Tb/s CPRI-equivalent data rate is transmitted in the system, satisfying the error vector magnitude (EVM) requirement for 256-quadrature amplitude modulation (QAM) signal transmission. With the symbol rate reduced to 10 Gbaud, an EVM below 2.5% is achieved, which meets the requirement for 1024-QAM transmission. The experimental results show that the coherent DA-RoF system is a promising solution for future fronthaul.

Air-gap Fabry-Pérot cavity filtered 30 nm broadband electro-optic frequency combs for high-order coherent communications.

Broadband electro-optic (EO) frequency combs, which have flexible and high repetition frequencies, are prospective light sources for dense-wavelength-division-multiplexed coherent optical communications. In most cases, nonlinear spectral broadening and amplification procedures are needed to achieve broadband and high-power EO frequency combs. This leads to a low optical carrier-to-noise ratio (OCNR) for comb lines, limiting the transmission capacity. Here, we propose to use an air-gap Fabry-Pérot (FP) cavity to improve the OCNR for all the comb lines covering a 30 nm broadband spectrum. A 12 dB OCNR (0.1 nm bandwidth) improvement is obtained experimentally via using an FP cavity with ∼790 MHz bandwidth. We apply a 150-channel filtered EO comb with 25 GHz channel spacing and load 20 GBaud signals on each comb line to demonstrate the effect of OCNR improvement. The 137/150 channels have a bit error rate below the threshold of soft-decision forward error correction when using the 128 quadrature amplitude modulation (QAM) format. However, none of these channels can support this modulation format without cavity filtering. We also investigate dispersion tolerance and the long-term stability when using an air-gap FP cavity, highlighting its advantages. Our results show a practical solution to boost the transmission capacity when applying broadband EO combs in optical communications.

Cascaded digital-analog radio-over-fiber for efficient SNR scaling at >10†dB per extra bandwidth.

Efficient signal-to-noise ratio (SNR) and spectral efficiency (SE) trade-off can offer fundamental guiding law for future large-capacity and high-fidelity mobile fronthaul. In this Letter, we propose and experimentally demonstrate a cascaded digital-analog radio-over-fiber (CDA-RoF) scheme, which transmits digitized and analog segments using time-division-multiplexing aggregation. Specifically, multiple digital parts generated by a rounding function describe the main features of the original waveform, while the residual error is magnified and delivered as analog RoF with SE advantage. Based on a 20-GHz O-band directly modulated laser (DML), an SNR enhancement of >10 dB is observed for each extra bandwidth. Up to 70.4-dB SNR is also achieved for a 3.5-GHz 1048576-ary quadrature amplitude modulation (1048576-QAM) signal after 20-km standard single-mode fiber (SSMF) transmission.

Direct detection transmission of a PAM signal with power fading mitigation based on Alamouti coding and dual-drive MZM.

Power-fading impairment induced by fiber dispersion and photodiode detection imposes a fundamental limitation on the intensity-modulation direct-detection (IM-DD) transmission systems. In this work, we propose a cost-effective pulse-amplitude modulation (PAM) signal transmission scheme with power-fading mitigation enabled by Alamouti coding and dual-drive Mach-Zehnder modulator (DDMZM). By interleaving the symbol blocks in the time domain for upper- and lower-arm of the DDMZM, flat end-to-end frequency response can be obtained without spectral nulls after combining the photocurrents at odd and even time slots. For single channel demonstration, we experimentally transmit up to 160Gb/s PAM-4, 140Gb/s PAM-6, and 108Gb/s PAM-8 signals over 80 km standard single-mode fiber (SSMF) with bit-error rates (BERs) below the 20% soft-decision forward error correction (SD-FEC) threshold of 2.0×10-2. For wavelength division multiplexing (WDM) transmission, 8λ×150Gb/s PAM-4 signals spacing at 100 GHz can also achieve 80 km reach. Moreover, we compare the optical signal-to-noise ratio (OSNR) sensitivity with single sideband (SSB) scheme and evaluate the tolerance of bias deviation in numerical simulation. The proposed Alamouti coding-based scheme provides a hardware-efficient and dispersion-tolerant candidate for high-speed inter-data center interconnect (DCI) applications.