Neurotrophic Effect of Magnesium Comenate in Normal and under Conditions of Oxidative Stress in Culture of Chicken Spinal Ganglia.

The neurotrophic properties of magnesium comenate were studied under standard conditions and under conditions of oxidative stress. It was found that magnesium comenate has a stimulating effect on the neurotrophic processes of the spinal ganglia under normal conditions and under conditions of oxidative stress. Under standard conditions, magnesium comenate exhibits neurotrophic activity at a concentration of 0.0001 mM, under conditions of oxidative stress, magnesium comenate exhibits neurotrophic activity at concentration 0.1 mM.

Neurotrophic Action of Comenic Acid and Its Derivatives Potassium Comenate and Calcium Comenate.

Using the model of cultured spinal ganglia, we demonstrated high neurotrophic activity of comenic acid and its derivatives potassium comenate and calcium comenate both under normal conditions and during oxidative stress. Calcium comenate in the norm as well as potassium and calcium comenates during oxidative stress demonstrate greater neurotrophic potency than comenic acid.

Antioxidant and Antiamnestic Effects of Potassium Comenate and Comenic Acid under Conditions of Normobaric Hypoxia with Hypercapnia.

Potassium comenate and comenic acid exhibit manifest and virtually identical antioxidant activity under conditions of hypoxia with hypercapnia. The effects of these drugs on conditioned reflex training with positive reinforcement differ significantly. Potassium comenate promotes retention of the learning capacity and memory in hypoxic rats at the level of intact control, that is, exhibits a pronounced protective antiamnestic effect, while comenic acid only facilitates training of the conditioned reflex with positive reinforcement.

Effect of Potassium Comenate on CNS Functional Status in Rodents Exposed to Combined Hypoxia and Hypercapnia in Comparison with Normally Ventilated Animals.

The effects of potassium comenate on functional state of CNS in mice and rats were studied in the open-field and hole-board tests under control conditions and after acute exposure to hypoxia-hypercapnia. The effects of potassium comenate on CNS were also studied in rodents subjected to propofol-induced sleep. Preliminary administration of 4 mg/kg potassium comenate for 3 days attenuated the posthypoxic changes in behavioral reactions (emotional anxiety/reactivity). The pronounced stress-protective effect of potassium comenate was observed both on days 1 and 14 after exposure to hypoxia-hypercapnia. Under normal conditions, potassium comenate moderated behavioral reactions and augmented somniferous effect of propofol. We hypothesized that the antihypoxic effect of potassium comenate is determined by its stress-protective and sedative potencies.

Comparative Analysis of Antioxidant Properties of Comenic Acid and Potassium Comenate in Modeled Immobilization Stress.

We studied antioxidant protective effect of comenic acid and potassium comenate in doses of 2, 4, and 8 mg/kg under conditions of oxidative stress in the brain of mice exposed to immobilization stress. Administration of potassium comenate and comenic acid in the above doses for 3 days before stress prevented the development of oxidative processes in the brain of stressed animals. The antioxidant effect of potassium comenate more pronounced.

Neuroprotective effect of potassium comenate against glutamate toxicity on the model of cultured rat cerebellar neurons.

The study demonstrated neuroprotective action of novel chemical agent, potassium salt of comenic acid, against the glutamate-induced cytotoxicity on the model of cultured cerebral neurons. Potassium comenate (0.001-1.0 mM) significantly decreased the rate of glutamateinduced neuronal death. The highest viability of the cultured neurons during postglutamate time was observed when potassium comenate was applied in a concentration of 0.1 mM.

[Influence of lithium salt of comenic acid on the state of oxidative processes in brain of stressed animals].

The influence of a new substance, lithium salt of comenic acid, on the oxidative processes in the brain of animals under the conditions of acute and long combined stress has been investigated. It is established that lithium comenate (1 and 2 mg/kg) exhibits pronounced dose-dependent antioxidative stress-protective effect, which is manifested in suppression of the hyperproduction of free radicals and depression of the content of malonic dialdehyde (a secondary product of lipid peroxidation) in the brain of stressed animals. The maximum antioxidative stress protection effect under the conditions of acute and long combined stress has been observed for lithium salt ofcomenic acid in a dose of 2 mg/kg.

[Influence of the peptides, isolated from kumys and a sour milk of Mechikov on some parameters of immunity in animals, who underwent an immobilization-cold stress].

The influence of peptide fractions, discharged of kumys (SK) and a sour milk of Mechnikov (PMS) on some parameters of immunity experimental animal, transferred a stress is investigated. The outcomes of examinations have shown, that SK promotes correction of immune violations called by action of a stress. So the introduction SK animal one day prior to a stress renders expressed immunoregulatory an operation on a content of neutrocytes and metabolism in them, depending from Oxygenium, promotes a raise of number of T lymphocytes in a blood animal transferred a stress. The peptide fraction, discharged of a sour milk of Mechnikov (PMS) does not render essential influence on the above-stated parameters.

[Various biochemical indices of wound healing during treatment with preparation SK]. / Nekotorye biokhimicheskie pokazateli ranevogo protsessa pri deistvii preparata SK.

There was investigated that the change of isoenzyme spectrum of laktatdegidrogenaze (LDG) in the dynamic of rats skin wounds healing, and also the activity of some antioxidative enzymes and the accumulation of one lipid peroxidation product (LJO). It was discovered that the enzyme spectrum LDG was reconstructed faster with treated animals by SK preparation. The phase of antioxidative enzymes activity and accumulation of some LFO products were recognized. SQ, the increasing of the katalaza activity in group, where the SK was used.

[Analysis of the immunomodulating action of SK in mice of various strains]. / Analiz immunomoduliruiushchego deistviia SK u mushei raznykh linii.

Peculiarities of immunomodulative action of different mice breeds' SK have been determined by changes of activity level of 5'-nucleotidase in macrophages from peritoneal exudate (MPE) and by changes of animals' sensitivity to staphylococcus infection. It has been established that the character of SK influence on activity level of 5'-nucleotidase in MPE and resistance depends on dose, type of administration and the mice breed. Revealed by hypodermic injection, dependence between the activity of 5'-nucleotidase in MPE and sensitivity to staphylococcus infection, whose character was determined by animals' genotype, had straight character in C57Bl/6 mice, and reverse character in CBA mice.

[Application of lytic enzymes for disruption of microbial cell walls to isolate DNA]. / Primenenie preparata liticheskikh fermentov dlia razrusheniia kletochiykh stenok mikroorganizmov s-tsel'iu vydeleniia iz nikh DNK.

The possibility of isolating DNA from yeast and actinomycete cells, previously exposed to enzymic disruption, was explored. The enzyme was produced from Actinomyces griseinus II. The highest yield of DNA from microbial cells was obtained as a result of detergent pretreatment of the yeast and actinomycetes and subsequent disruption of cell walls with the above enzyme.