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1.
Res Microbiol ; 171(5-6): 211-214, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32198075

RESUMEN

Mycobacterium abscessus (MAB) comprise rapidly growing, often multidrug-resistant (MDR), nontuberculous mycobacteria responsible for pulmonary and other infections in susceptible hosts. Antimicrobial peptides (APs) are natural and synthetic antimicrobials active against a range of microorganisms including mycobacteria. We evaluated APs activity against MAB reference and clinical strains. We observed minimal inhibitory concentrations of 1.6 to >50 µg/mL. Further work with the most active AP demonstrated protection of Acanthamoeba castellanii (AC) from killing by ingested MAB including MDR MAB strains. Antimicrobial peptides offer an attractive potential option for treatment of drug resistant treatment-refractory MAB.


Asunto(s)
Antibacterianos/farmacología , Mycobacterium abscessus/efectos de los fármacos , Proteínas Citotóxicas Formadoras de Poros/farmacología , Acanthamoeba castellanii/microbiología , Farmacorresistencia Bacteriana , Humanos , Pruebas de Sensibilidad Microbiana , Infecciones por Mycobacterium no Tuberculosas/microbiología
2.
Res Microbiol ; 169(1): 56-60, 2018 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-29056478

RESUMEN

We used an amoeba model to study the intracellular growth and cytotoxicity of clinical strains of Mycobacterium abscessus subsp. massiliense (Mabsm) isolated from 2 patients (one with cystic fibrosis, the other one with idiopathic bronchiectasis) during the early (smooth colonies) and late stage (rough colonies) of chronic pulmonary infection. Acanthamoeba castellanii were infected with Mabsm (MOI 100) and samples collected every 24 h for 72 h. Results showed Mabsm is able to survive in trophozoites and persist in cysts for at least 7 days. Late Mabsm demonstrated higher cytotoxicity toward A. castellanii when compared to early strains. A. castellanii is a useful in vitro host model to study infection of Mabsm clinical isolates.


Asunto(s)
Acanthamoeba castellanii/microbiología , Viabilidad Microbiana , Infecciones por Mycobacterium no Tuberculosas/microbiología , Mycobacterium abscessus/crecimiento & desarrollo , Acanthamoeba castellanii/fisiología , Humanos , Modelos Animales , Mycobacterium abscessus/fisiología
3.
Genome Announc ; 3(3)2015 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-25999579

RESUMEN

We report the whole-genome sequence (WGS) of an in vitro susceptible derivative revertant mutant from a bloodstream isolate involved in a nosocomial outbreak in Brazil. The WGS comprises 2.5 Mb with 2,500 protein-coding sequences, 16rRNA genes, and 60 tRNA genes.

4.
FEMS Microbiol Lett ; 344(2): 166-72, 2013 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-23651353

RESUMEN

Bacteriophage Recombineering of Electroporated DNA (BRED) has been described for construction of gene deletion and point mutations in mycobacteriophages. Using BRED, we inserted a Phsp60-egfp cassette (1143 bp) into the mycobacteriophage D29 genome to construct a new reporter phage, which was used for detection of mycobacterial cells. The cassette was successfully inserted and recombinant mycobacteriophage purified. DNA sequencing of the cassette did not show any mutations even after several phage generations. Mycobacterium smegmatis mc(2) 155 cells were infected with D29::Phsp60-egfp (MOI of 10) and evaluated for EGFP expression by microscopy. Fluorescence was observed at around 2 h after infection, but dissipated in later times because of cell lysis. We attempted to construct a lysis-defective mutant by deleting the lysA gene, although we were unable to purify the mutant to homogeneity even with complementation. These observations demonstrate the ability of BRED to insert c. 1 kbp-sized DNA segments into mycobacteriophage genomes as a strategy for constructing new diagnostic reporter phages.


Asunto(s)
Genes Reporteros , Proteínas Fluorescentes Verdes/genética , Micobacteriófagos/genética , Mycobacterium smegmatis/genética , Mycobacterium smegmatis/virología , Electroporación , Proteínas Fluorescentes Verdes/metabolismo , Lisogenia , Micobacteriófagos/fisiología , Mycobacterium smegmatis/metabolismo , Regiones Promotoras Genéticas , Eliminación de Secuencia
5.
Braz. j. microbiol ; 40(3): 547-549, Sept. 2009.
Artículo en Inglés | LILACS | ID: lil-522475

RESUMEN

Bacteriophages have been researched as a new alternative to antibiotics. These viruses inject their genetic material into bacteria and use their host machinery to multiply themselves. The research of bacteriophages in Brazil will certainly provide low-cost treatment of multidrug resistant bacteria, new microbiological diagnosis and advantages for the Brazilian food industry.


Bacteriófagos têm sido pesquisados como uma alternativa ao uso de antibióticos. Estes vírus infectam as bactérias e utilizam a maquinaria celular para multiplicar o próprio material genético. O estudo de bacteriófagos no Brasil levará ao desenvolvimento de tratamentos de baixo custo, novos testes diagnósticos e vantagens para a industria alimentícia.

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