The use of single phrenic axon recordings to assess diaphragm recovery after cervical spinal cord injury.

Electrophysiological recordings taken from the whole phrenic nerve have been utilized previously to describe the gradual increase in functional recovery of a hemidiaphragm paralyzed by ipsilateral C2 hemisection during the crossed phrenic phenomenon (CPP). Although the increase in activity has been temporally correlated with hemisection-induced morphological alterations of the phrenic nucleus, suggesting an association of the increased activity with the morphological alterations, whole phrenic nerve recordings during the CPP can provide only limited information. The purpose of the present study, therefore, was to use phrenic single-axon recording techniques to better understand the mechanisms underlying the recovery of respiratory activity during the expression of the CPP. Recordings from the whole phrenic nerve on the right side and from small fascicles of the phrenic nerve that show only the activity of single phrenic axons (units) on the left side were made in the neck before left spinal hemisection and during the CPP. The results indicated that there were two types of units firing before and during the CPP: an early- and a late-firing unit based on the time of their firing onset in relation to whole phrenic nerve activity. Ten early units and 25 late units were identified according to the shape of their spikes before hemisection as well as during the CPP. In addition to these units, 20 new units were recruited during CPP activity. These new units were mainly of the late-onset type. The results also indicated that there was a significant increase in the frequency of firing of both early and late units. The results specifically indicate therefore that the increase in respiratory activity recorded previously in the whole phrenic nerve during the CPP is most likely due to: (i) an increase in firing frequency for both early- and late-firing units and (ii) a recruitment of predominantly late-firing units into the CPP response. These results are important in understanding more completely the mechanisms that can facilitate recovery of the diaphragm after spinal cord injury.

Quantitative assessment of respiratory function following contusion injury of the cervical spinal cord.

In this study, we describe a new method for quantitative assessment of phrenic inspiratory motor activity in two models of cervical spinal cord contusion injury. Anesthetized rats received contusion injury either to the descending bulbospinal respiratory pathway on one side of the spinal cord alone (C2 lateralized contusion) or to both the descending pathway, as well as the phrenic motoneuron pool bilaterally (C4/C5 midline contusion). Following injury, respiratory-associated phrenic nerve motor activity was recorded under standardized and then asphyxic conditions. Phrenic nerve efferent activity was rectified, integrated, and quantitated by determining the mean area under the integrated neurograms. The mean integrated area of the four inspiratory bursts recorded just before turning off the ventilator (to induce asphyxia) was determined and divided by the integrated area under the single largest respiratory burst recorded during asphyxia. This latter value was taken as the maximal inspiratory motor response that the rat was capable of generating during respiratory stress. Thus, a percentage of the maximal inspiratory motor drive was established for breathing in control and injured rats under standardized conditions. The results indicate that noninjured rats use 52 +/- 1.8% of maximal inspiratory motor drive under standardized conditions. In C2-contused rats, the results showed that while the percentage of maximal inspiratory motor drive on the noncontused side was similar to the control (55 +/- 4.1%), it was increased on the contused side (78 +/- 2.6%). In C4/5 lesions, the results indicate that this percentage was increased on both sides (77 +/- 4.4%). The results show the feasibility for performing quantitative evaluation of respiratory dysfunction in an animal model of cervical contusion injury. These findings lend to further development of this model for investigations of neuroplasticity and/or therapeutic interventions directed at ameliorating respiratory compromise following cervical spinal cord trauma.

Mechanosensitive afferent units in the lumbar facet joint.

The purpose of this study was to characterize somatosensory units of the lumbar facet joint, which may play a central role in idiopathic low-back pain. A laminectomy was performed on the lumbar spine of adult male New Zealand White rabbits. Receptive fields of mechanosensitive afferent units were investigated in the lumbar facet joint and adjacent surrounding tissues, and electrophysiological recordings were obtained from filaments of the dorsal root. Twenty-four units were identified in the region of the facet joint: ten, in the capsule of the joint; twelve, in the border regions between capsule and muscle or tendon; and two, in the ligamentum flavum. Of these units, two had a conduction velocity that was slower than 2.5 meters per second (group IV), fifteen had a velocity ranging from 2.5 to twenty meters per second (group III), and seven had a velocity faster than twenty meters per second. Seven units had a von Frey threshold of more than 6.0 grams, thirteen had a threshold of less than 6.0 grams, and four were not examined. Seven units in the facet joint responded to movement of the joint. Fourteen other mechanosensitive units were found in the muscle, tendon, and interspinous ligament; seven had a conduction velocity of 2.5 to twenty meters per second, and seven had a velocity that was faster than twenty meters per second.

Experimental verification of facet load transmission by direct measurement of facet lamina contact pressure.

This paper presents results of a new measuring technique by means of which facet tip contact pressure data were obtained. The aim of the paper is to demonstrate by direct measurement the existence of a load transmission mechanism through the facet joint. Six lumbar spine motion segments were used in 21 tests. Simulated extensor muscle action was provided to overcome moments due to eccentric loads which represented body weight and an external hand-held load acting 340 mm anterior to the center of the disc. Facet pressure was measured in all cases when muscle load was applied to counteract body weight. This pressure increased when more muscle force was applied to balance the externally applied flexion moment. When the anterior load was released suddenly, there was a large increase in facet pressure with a concomitant decrease in disc pressure.