1.
Appl Environ Microbiol
; 70(3): 1869-73, 2004 Mar.
Artículo
en Inglés
| MEDLINE
| ID: mdl-15006820
RESUMEN
Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase.