The Nuances of Intimacy: Asexual Perspectives and Experiences with Dating and Relationships.

Asexuality studies are an emerging field in North America and Europe which lack large-scale, qualitative studies. Much existing research focuses on defining and categorizing asexuality, with researchers calling for more focus on the romantic and sexual relationships of asexual people. Drawing from an online survey with 349 participants, this paper describes the perceptions and experiences that asexual people have with dating and relationships. Participants answered 16 open-ended and 9 close-ended questions. After rounds of coding, three themes were selected for examination. These were dating, romantic and/or sexual relationships, and platonic relationships. These results showcase the challenges asexuals face with dating while also demonstrating how asexual people actively dismantle understandings of romantic, sexual, and platonic relationships through their own experiences and perspectives. Overall, this study provides greater legitimacy to the fluidity of asexuality and asexual relationships.

Transcriptomic basis of sex loss in the pea aphid.

BACKGROUND: Transitions from sexual to asexual reproduction are common in eukaryotes, but the underlying mechanisms remain poorly known. The pea aphid-Acyrthosiphon pisum-exhibits reproductive polymorphism, with cyclical parthenogenetic and obligate parthenogenetic lineages, offering an opportunity to decipher the genetic basis of sex loss. Previous work on this species identified a single 840 kb region controlling reproductive polymorphism and carrying 32 genes. With the aim of identifying the gene(s) responsible for sex loss and the resulting consequences on the genetic programs controlling sexual or asexual embryogenesis, we compared the transcriptomic response to photoperiod shortening-the main sex-inducing cue-of a sexual and an obligate asexual lineage of the pea aphid, focusing on heads (where the photoperiodic cue is detected) and embryos (the final target of the cue). RESULTS: Our analyses revealed that four genes (one expressed in the head, and three in the embryos) of the region responded differently to photoperiod in the two lineages. We also found that the downstream genetic programs expressed during embryonic development of a future sexual female encompass ∼1600 genes, among which miRNAs, piRNAs and histone modification pathways are overrepresented. These genes mainly co-localize in two genomic regions enriched in transposable elements (TEs). CONCLUSIONS: Our results suggest that the causal polymorphism(s) in the 840 kb region somehow impair downstream epigenetic and post-transcriptional regulations in obligate asexual lineages, thereby sustaining asexual reproduction.

Parthenogenomics: Insights on mutation rates and nucleotide diversity in parthenogenetic Panagrolaimus nematodes.

Asexual reproduction is assumed to lead to the accumulation of deleterious mutations, and reduced heterozygosity due to the absence of recombination. Panagrolaimid nematode species display different modes of reproduction. Sexual reproduction with distinct males and females, asexual reproduction through parthenogenesis in the genus Panagrolaimus, and hermaphroditism in Propanagrolaimus. Here, we compared genomic features of free-living nematodes in populations and species isolated from geographically distant regions to study diversity, and genome-wide differentiation under different modes of reproduction. We firstly estimated genome-wide spontaneous mutation rates in a triploid parthenogenetic Panagrolaimus, and a diploid hermaphroditic Propanagrolaimus via long-term mutation accumulation lines. Secondly, we calculated population genetic parameters including nucleotide diversity, and fixation index (F ST) between populations of asexually and sexually reproducing nematodes. Thirdly, we used phylogenetic network methods on sexually and asexually reproducing Panagrolaimus populations to understand evolutionary relationships between them. The estimated mutation rate was slightly lower for the asexual population, as expected for taxa with this reproductive mode. Natural polyploid asexual populations revealed higher nucleotide diversity. Despite their common ancestor, a gene network revealed a high level of genetic differentiation among asexual populations. The elevated heterozygosity found in the triploid parthenogens could be explained by the third genome copy. Given their tendentially lower mutation rates it can be hypothesized that this is part of the mechanism to evade Muller's ratchet. Our findings in parthenogenetic triploid nematode populations seem to challenge common expectations of evolution under asexuality.

Deepening Sexual Desire and Erotic Fantasies Research in the ACE Spectrum: Comparing the Experiences of Asexual, Demisexual, Gray-Asexual, and Questioning People.

Over the past 30 years, an increasing number of people have identified within the asexual (ACE) spectrum recognizing an absence/low/situational sexual attraction to individuals of any gender. The current study aims to deepen the knowledge of sexual desire, erotic fantasies, and related emotions within the ACE spectrum. A total of 1072 Italian volunteers were recruited to take part to the present study via social media. Data were collected from October 2021 to January 2022 using the Sexual Desire and Erotic Fantasies questionnaire and the Sexual Desire Inventory-2. Participants were divided into four groups: asexual, demisexual, gray-asexual, and questioning. Focusing on sexual desire, asexual people reported significantly lower scores than the other groups in all the dimensions except for "negative feelings to sexual desire," while demisexual participants showed the higher scores in all the domains except for "negative feelings to sexual desire." The questioning group reported the highest scores in the "negative feelings toward sexual desire" compared to the asexual and demisexual ones. The asexual group reported significantly lower scores than the other groups in fantasies frequency, fantasies importance, negative emotions, and sharing and experiencing. The demisexual group showed higher frequency of romantic fantasies than the asexual and gray-asexual ones. The results showed some specific patterns of desire and fantasies among the asexual, gray-asexual, demisexual, and questioning groups. These data may provide relevant material to clinicians working with asexual patients who need greater awareness about the diversity and heterogeneity of the sexual experience within the ACE spectrum.

A Comparison of Mate Preferences in Asexual and Allosexual Adults.

Asexuality is a sexual orientation characterized by a lack of sexual attraction. Sexual attraction is likely associated with preferences for certain characteristics in romantic partners, such as physical attractiveness. Preferred partner characteristics can be influenced by an individual's sexual orientation, gender, and age. Allosexual (N = 239; male = 48, female = 181, other = 4; Age M = 20.48 years) and asexual participants (N = 149; male = 36, female = 88, other = 23; Age M = 25.54 years) recruited from a pool of psychology students and through online asexual communities were presented with a survey in which a total of 388 participants rated 13 characteristics according to how desirable they were in a potential long-term romantic partner. Characteristics that are related to physical attractiveness were predicted to be rated lower by asexual participants than by allosexual participants. Asexual participants rated the desire to have children as being less desirable in a romantic partner than allosexual participants did. However, preferences for other traits, such as exciting personality, creative and artistic, and religious, were dependent on interactions of gender and attraction to men or women. Because asexual individuals report generally lower levels of sexual attraction, it will be important for future research to consider romantic attraction as a more nuanced measure than sexual orientation alone when considering sex differences in asexual and allosexual populations.

"I am not a Robot, I am Asexual": A Qualitative Critique of Allonormative Discourses of Ace and Aro Folks as Robots, Aliens, Monsters.

Despite exploring other queer topics, communication scholars and rhetoricians are lacking in offering academic inquiries involving asexuality. We engaged in a critical thematic analysis of posts and comments posted from asexual and/or aromantic folks on the online communities of Reddit and AVEN, the Asexuality Visibility & Education Network. The analysis focused on three cultural tropes prominently used when describing asexual and aromantic folks, namely robots, aliens, and monsters. Our discussion centers asexual and aromantic voices and invites communication and rhetorical scholars to take steps in combating the allonormativity of our fields by centering the voices and experiences of intersectional aces and/or aros in their own research. We conclude with a call for an asexual and aromantic communication studies.

Análisis de la actividad sexual en el adulto mayor del Policlínico Universitario Este

Introducción: El contexto social en el cual se desenvuelve la mayoría de los adultos mayores los conduce a situaciones de riesgo, condiciones o problemas de salud con consecuencias físicas, psicológicas y sociales específicas que afectan también su sexualidad y que deben ser abordadas en la práctica. Objetivo: Analizar la actividad sexual en el adulto mayor del Policlínico Universitario Este, entre enero de 2021 y julio de 2023. Métodos: Se realizó un estudio observacional, analítico, transversal, en el Policlínico Universitario Este en la provincia Camagüey. El universo estuvo constituido por 9 936 pacientes según los criterios de inclusión y de exclusión. Se trabajó 154 pacientes seleccionados mediante el muestreo aleatorio simple. Los expedientes clínicos ambulatorios fueron la fuente secundaria de información. Se utilizó estadística descriptiva e inferencial. Los datos se presentaron en tablas y gráficos. Resultados: Los mayores de 74 años representaron el 13,6 %, de ellos; el 8,4 % mostraron actividad sexual inactiva. El 48,7 % eran del sexo masculino. Los que no tuvieron atracción sexual fueron el 9,7 %. De los pacientes estudiados con actividad sexual activa 33, para un 21,4 %, presentan actividad sexual quincenal, mientras que 24 (15,6 %) semanal. Conclusiones: Los trastornos neurológicos como enfermedades asociadas, así como los fármacos inhibidores de la excitación sexual acompañado del antecedente de disfunción sexual como factores de riesgo imposibilitaron el desarrollo normal de la actividad sexual en los longevos estudiados. El bajo apetito sexual y falta de atracción sexual, así como el estado de viudez se asociaron significativamente a la inactividad sexual.

Introduction: The social context in which the majority of older adults live leads them to risk situations, conditions or health problems with specific physical, psychological and social consequences that also affect their sexuality and that must be addressed in practice. Objective: To analyze sexual activity in older adults at the East University Polyclinic between January 2021 and July 2023. Methods: An observational, analytical, cross-sectional study was carried out in the East University Polyclinic. The universe consisted of 9936 patients according to the inclusion and exclusion criteria. It was worked with 103 patients selected through simple random sampling. Outpatient clinical records were the secondary source of information. Descriptive and inferential statistics were used. The data was presented in tables and graphs. Results: Those over 74 years of age represented 13.6 % of them; 8.4 % showed inactive sexual activity. 48.7 % were male. Those who did not have sexual attraction were 9.7 %. Of the patients studied with active sexual activity, 33, or 21.4 %, had sexual activity every two weeks, while 24 (15.6 %) had sexual activity weekly. Conclusions: Neurological disorders as associated diseases, as well as drugs that inhibit sexual arousal, accompanied by a history of sexual dysfunction as risk factors, prevented the normal development of sexual activity in the elderly studied. Low sexual appetite and lack of sexual attraction, as well as widowhood status, were significantly associated with sexual inactivity.

Contrasting Evolutionary Patterns Between Sexual and Asexual Lineages in a Genomic Region Linked to Reproductive Mode Variation in the pea aphid.

Although asexual lineages evolved from sexual lineages in many different taxa, the genetics of sex loss remains poorly understood. We addressed this issue in the pea aphid Acyrthosiphon pisum, whose natural populations encompass lineages performing cyclical parthenogenesis (CP) and producing one sexual generation per year, as well as obligate parthenogenetic (OP) lineages that can no longer produce sexual females but can still produce males. An SNP-based, whole-genome scan of CP and OP populations sequenced in pools (103 individuals from 6 populations) revealed that an X-linked region is associated with the variation in reproductive mode. This 840-kb region is highly divergent between CP and OP populations (FST = 34.9%), with >2,000 SNPs or short Indels showing a high degree of association with the phenotypic trait. In OP populations specifically, this region also shows reduced diversity and Tajima's D, consistent with the OP phenotype being a derived trait in aphids. Interestingly, the low genetic differentiation between CP and OP populations at the rest of the genome (FST = 2.5%) suggests gene flow between them. Males from OP lineages thus likely transmit their op allele to new genomic backgrounds. These genetic exchanges, combined with the selection of the OP and CP reproductive modes under different climates, probably contribute to the long-term persistence of the cp and op alleles.

Evidence for cryptic sex in parthenogenetic stick insects of the genus Timema.

Obligately parthenogenetic species are expected to be short lived since the lack of sex and recombination should translate into a slower adaptation rate and increased accumulation of deleterious alleles. Some, however, are thought to have been reproducing without males for millions of years. It is not clear how these old parthenogens can escape the predicted long-term costs of parthenogenesis, but an obvious explanation is cryptic sex. In this study, we screen for signatures of cryptic sex in eight populations of four parthenogenetic species of Timema stick insects, some estimated to be older than 1 Myr. Low genotype diversity, homozygosity of individuals and high linkage disequilibrium (LD) unaffected by marker distances support exclusively parthenogenetic reproduction in six populations. However, in two populations (namely, of the species Timema douglasi and T. monikensis) we find strong evidence for cryptic sex, most likely mediated by rare males. These populations had comparatively high genotype diversities, lower LD, and a clear LD decay with genetic distance. Rare sex in species that are otherwise largely parthenogenetic could help explain the unusual success of parthenogenesis in the Timema genus and raises the question whether episodes of rare sex are in fact the simplest explanation for the persistence of many old parthenogens in nature.

Why do hybrids turn down sex?

Asexual reproduction is ancestral in prokaryotes; the switch to sexuality in eukaryotes is one of the major transitions in the history of life. The study of the maintenance of sex in eukaryotes has raised considerable interest for decades and is still one of evolutionary biology's most prominent question. The observation that many asexual species are of hybrid origin has led some to propose that asexuality in hybrids results from sexual processes being disturbed because of incompatibilities between the two parental species' genomes. However, in some cases, failure to produce asexual F1s in the lab may indicate that this mechanism is not the only road to asexuality in hybrid species. Here, we present a mathematical model and propose an alternative, adaptive route for the evolution of asexuality from previously sexual hybrids. Under some reproductive alterations, we show that asexuality can evolve to rescue hybrids' reproduction. Importantly, we highlight that when incompatibilities only affect the fusion of sperm and egg's genomes, the two traits that characterize asexuality, namely unreduced meiosis and the initiation of embryogenesis without the incorporation of the sperm's pronucleus, can evolve separately, greatly facilitating the overall evolutionary route. Taken together, our results provide an alternative, potentially complementary explanation for the link between asexuality and hybridization.

Beyond sexual deviance: Elevating the expansive intimacies of Chicana lesbian life in Chicana Lesbians: The Girls Our Mothers Warned Us About.

In this article, I expand popular readings of Chicana lesbianism focused on sexuality by tending more deeply to the affective terrains of love and kinship represented in the 1991 anthology Chicana Lesbians: The Girls Our Mothers Warned Us About edited by Carla Trujillo. Countering the (il)logics of white supremacy and Chicano nationalism which reduce Chicana lesbians to symbols of sexual deviance, I argue that Chicana Lesbians embodies an expansive matrix of intimacies that reconstruct the Chicana lesbian figure from a one-dimensional symbol of sexual deviance to a multi-faceted figure who redefines what it means to love one's people and culture beyond colonial paradigms that privilege heterosexuality. Drawing upon theories of decolonial love and queer asexuality, I examine the expansive inner lives and intimacies of Chicana lesbians to construct a more thorough portrait of how we love and relate to each other. While many studies foreground the sexual lives and politics of Chicana lesbians as subversive to the heteronormative status quo, I elevate the equally powerful forces of love and kinship in our struggle to transform the legacies of colonialism and Chicano nationalism.

The Transcriptomic Signature of Cyclical Parthenogenesis.

Cyclical parthenogenesis, where females can engage in sexual or asexual reproduction depending on environmental conditions, represents a novel reproductive phenotype that emerged during eukaryotic evolution. The fact that environmental conditions can trigger cyclical parthenogens to engage in distinct reproductive modes strongly suggests that gene expression plays a key role in the origin of cyclical parthenogenesis. However, the genetic basis underlying cyclical parthenogenesis remains understudied. In this study, we characterize the female transcriptomic signature of sexual versus asexual reproduction in the cyclically parthenogenetic microcrustacean Daphnia pulex and Daphnia pulicaria. Our analyses of differentially expressed genes (DEGs), pathway enrichment, and gene ontology (GO) term enrichment clearly show that compared with sexual reproduction, the asexual reproductive stage is characterized by both the underregulation of meiosis and cell cycle genes and the upregulation of metabolic genes. The consensus set of DEGs that this study identifies within the meiotic, cell cycle, and metabolic pathways serves as candidate genes for future studies investigating how the two reproductive cycles in cyclical parthenogenesis are mediated at a molecular level. Furthermore, our analyses identify some cases of divergent expression among gene family members (e.g., doublesex and NOTCH2) associated with asexual or sexual reproductive stage, suggesting potential functional divergence among gene family members.

No recombination suppression in asexually produced males of Daphnia pulex.

Obligate parthenogenesis (OP) is often thought to evolve by disruption of reductional meiosis and suppression of crossover recombination. In the crustacean Daphnia pulex, OP lineages, which have evolved from cyclical parthenogenetic (CP) ancestors, occasionally produce males that are capable of reductional meiosis. Here, by constructing high-density linkage maps, we find that these males show only slightly and nonsignificantly reduced recombination rates compared to CP males and females. Both meiosis disruption and recombination suppression are therefore sex-limited (or partly so), which speaks against the evolution of OP by disruption of a gene that is essential for meiosis or recombination in both sexes. The findings may be explained by female-limited action of genes that suppress recombination, but previously identified candidate genes are known to be expressed in both sexes. Alternatively, and equally consistent with the data, OP might have evolved through a reuse of the parthenogenesis pathways already present in CP and through their extension to all events of oogenesis. The causal mutations for the CP to OP transition may therefore include mutations in genes involved in oogenesis regulation and may not necessarily be restricted to genes of the "meiosis toolkit." More generally, our study emphasizes that there are many ways to achieve asexuality, and elucidating the possible mechanisms is key to ultimately identify the genes and traits involved.

Beliefs About the Inevitability of Sexual Attraction Predict Stereotypes About Asexuality.

Similar to other sexual minorities, asexual individuals often face prejudice and stereotyping. However, the source of these attitudes and beliefs is not well understood. We hypothesized that asexual stereotypes stem from the belief that sexual attraction is an inevitable part of human development. This attraction inevitability assumption can lead to the deduction that people who identify as asexual do so because they are going through a transitory stage or excusing socially avoidant tendencies. To test this stereotype deduction account, we examined whether specific asexual stereotypes (immaturity and non-sociality) were associated with adherence to the attraction inevitability assumption. Heterosexual participants (N = 322; 201 women, 114 men; mean age 34.6 yrs.) from the UK and the US read vignettes describing a target character that was either asexual or heterosexual. People who assumed that attraction is inevitable were more likely to evaluate asexual targets (but not heterosexual targets) as immature and non-social. The impact of the sexual inevitability assumption was present even when social dominance orientation, an attitude closely related to negativity toward all sexual minorities, was accounted for. Participants who adhered to the attraction inevitability assumption also showed a reduced inclination to befriend asexual individuals. These findings suggest that generalized negativity toward sexual minorities does not fully explain stereotypes and prejudice against asexual people. Instead, the current study highlights how perceived deviation from the shared understanding of sexuality uniquely contributes to anti-asexual bias.

Population Genomic Analyses Suggest a Hybrid Origin, Cryptic Sexuality, and Decay of Genes Regulating Seed Development for the Putatively Strictly Asexual Kingdonia uniflora (Circaeasteraceae, Ranunculales).

Asexual lineages are perceived to be short-lived on evolutionary timescales. Hence, reports for exceptional cases of putative 'ancient asexuals' usually raise questions about the persistence of such species. So far, there have been few studies to solve the mystery in plants. The monotypic Kingdonia dating to the early Eocene, contains only K. uniflora that has no known definitive evidence for sexual reproduction nor records for having congeneric sexual species, raising the possibility that the species has persisted under strict asexuality for a long period of time. Here, we analyze whole genome polymorphism and divergence in K. uniflora. Our results show that K. uniflora is characterized by high allelic heterozygosity and elevated πN/πS ratio, in line with theoretical expectations under asexual evolution. Allele frequency spectrum analysis reveals the origin of asexuality in K. uniflora occurred prior to lineage differentiation of the species. Although divergence within K. uniflora individuals exceeds that between populations, the topologies of the two haplotype trees, however, fail to match each other, indicating long-term asexuality is unlikely to account for the high allele divergence and K. uniflora may have a recent hybrid origin. Phi-test shows a statistical probability of recombination for the conflicting phylogenetic signals revealed by the split network, suggesting K. uniflora engages in undetected sexual reproduction. Detection of elevated genetic differentiation and premature stop codons (in some populations) in genes regulating seed development indicates mutational degradation of sexuality-specific genes in K. uniflora. This study unfolds the origin and persistence mechanism of a plant lineage that has been known to reproduce asexually and presents the genomic consequences of lack of sexuality.

Experiences of minority stress and their impact on suicidality among asexual individuals.

INTRODUCTION: While the asexual community has become increasingly visible, the pathologization and denial of asexuality remain strong, which may be linked to a higher prevalence of suicidality in this population. The present study examined experiences of minority stress and their relationship with suicidality among asexual individuals. METHODS: This study included a global sample of 12,449 individuals on the asexual spectrum. They completed an online survey assessing minority stress (including verbal aggression, victimization, and health care discrimination), impairment in different aspects of life, and suicidality. RESULTS: The results showed that 64.8 % of asexual individuals had experienced minority stress based on sexual and/or romantic orientation. Approximately 32.2 % reported suicidal ideation, 10.6 % had suicide plans, and 2.7 % had attempted suicide in the past 12 months. Suicidality was more prevalent among asexual men and non-binary individuals than among asexual women. Asexual individuals who were younger, identified as transgender, and lived in lower-income countries were more likely to report suicidality. Among the different dimensions of minority stress, victimization was most significantly associated with suicidal ideation, plans, and attempts. The positive association between minority stress and suicidality was mediated by impairment in health. LIMITATIONS: The cross-sectional nature of the data might prevent causal inferences from being drawn. Validated measures of asexual-specific minority stress were not available. CONCLUSION: The findings have implications for asexuality-affirming mental health practices supporting asexual individuals in coping with minority stress experiences. Public education efforts are needed to raise awareness of asexuality as a valid sexual orientation and to dispel misconceptions about asexuality.

Beyond sex: A review of recent literature on asexuality.

Asexuality has gained increasing attention in academic research and literature over the last two decades. This review discusses findings from 28 relevant studies published in academic journals between January 2020 and July 2022. In this time, academic literature on asexuality has focused primarily on 1) development of asexual identity, 2) sex and romantic relationships for asexual individuals, 3) intersections between asexuality and other aspects of identity, and 4) the need for increased awareness and understanding of asexuality. Future research should expand the scope of asexuality research to include more intersectional perspectives and more experiences of those on the ace spectrum who do not identify as asexual (e.g. graysexual, demisexual).

Understanding Attraction, Behavior, and Identity in the Asexual Community.

Models of sexuality have evolved substantially in the past several decades through the inclusion of new aspects which were previously overlooked. Components such as romantic attraction and behavior have also traditionally been included in models of sexuality. However, romantic and sexual orientations do not coincide for all individuals. A population for which this is true and one that has developed a robust language for discussing romantic orientation is the asexual community. The current study aimed to explore romantic and sexual orientation through patterns found within the factors of attraction, behavior, and identity in the asexual community. The current sample composed of individuals who identified as asexual (N = 306, Mage = 27.1) was 61% female, 13% non-binary, and 10% self-described or used multiple labels. Within this sample, aspects of sexual and romantic orientations and experiences were measured, including fluidity, the quantity and type of self-identified labels, desire for romance or sex, and the role of contextual influences on these experiences. These aspects were used as the primary characteristics to construct participant profiles, both complete profiles and factor specific (attraction, behavior, identity). t-distributed stochastic neighbor embedding (tSNE) was used to find patterns of similarity between individual participant profiles. Overall, it appeared that attraction was the factor most closely associated with overall experiences; however, substantial variability existed between participants. These findings provide a mechanism for better understanding of some nuances of romantic and sexual orientation and may be a useful first step toward future inquiry and hypothesis generation.