RESUMEN
A new ester (1) and a terpenoid (2) were isolated from the dried whole plant of Disporopsis aspersa (HUA) ENGL. ex DIELS for the first time and their structures were elucidated, as well as their biological activities are described. The two compounds all showed good antifungal activities, especially furanone (2) exhibited better antifungal activity against Pseudoperonospora cubensis and Phytophthora infestans with EC50 value of 22.82, 18.90 µg/mL, respectively. Compound 1 exhibited a significant promotion on the neurite outgrowth in NGF-induced PC-12 cells, and moderate inhibition on the NO production induced by lipopolysaccharide (LPS) in BV-2 microglial cells.
Asunto(s)
Antiinflamatorios/aislamiento & purificación , Antifúngicos/aislamiento & purificación , Asparagaceae/química , Proyección Neuronal/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Antiinflamatorios/farmacología , Antifúngicos/farmacología , Ésteres/aislamiento & purificación , Ésteres/farmacología , Microglía/efectos de los fármacos , Óxido Nítrico/antagonistas & inhibidores , Células PC12/efectos de los fármacos , Células PC12/ultraestructura , Phytophthora infestans/efectos de los fármacos , Extractos Vegetales/química , Ratas , Terpenos/aislamiento & purificación , Terpenos/farmacologíaRESUMEN
Oomycetes are one type of the most highly destructive of the diseases that cause damage to some important crop plants, such as potato late blight, cucumber downy mildew, and grape downy mildew. As main approach of the ongoing search for new botanical fungicide from plant, the secondary metabolites of D. aspersa were investigated. Through efficient bioassay-guided isolation, two new (1 and 2) and 12 known compounds (3 - 14) were isolated, and their structures were determined via extensive NMR, HR-ESI-MS, and IR. They were isolated from this genus for the first time except for compounds 11 and 12. The biological properties of 1 - 14 were evaluated against Pseudoperonospora cubensis and Phytophthora infestans. Compounds 1 - 8 showed potent antifungal activity in vitro. Additionally, compound 3 has preferable control effect on cucumber downy mildew, showing dual effect of protection and treatment in vivo.
