Metagenomics-based insights into the microbial community dynamics and flavor development potentiality of artificial and natural pit mud.

Strong-flavor Baijiu (SFB) production has relied on pit mud (PM) as a starter culture. The maturation time of natural PM (NPM) is about 30 years, so artificial PM (APM) with a shorter maturation time has attracted widespread attention. This study reveals the microbial and functional dissimilarities of APM and NPM, and helps to elucidate the different metabolic roles of microbes during substrate degradation and flavor formation. Significant differences in the microbial community were observed between APM and NPM, manifesting as variations in the abundance of core microorganisms. Total of 187 high-quality metagenome-assembled genomes (MAGs) were obtained based on the metagenomic binning technology, mainly including Firmicutes (n = 106), Bacteroidota (n = 15) and Chloroflexota (n = 14). Furthermore, the relative concentration of flavor compounds in 4-year APM was similar to those in 30-year NPM, but different from those in 100-year NPMs. Methanosarcina, Methanobacterium, Methanoculleus, Anaerolineae bacterium and Aminobacterium were the key bacteria responsible for the flavor differences. From a functional perspective, amino acid and carbohydrate metabolism were key functions of PM microbial, and showed differences between APM and NPM. Finally, substrate degradation and flavor generation pathways were found to exist in multiple microorganisms. Combine the relative abundance of microorganisms with the absolute abundance of enzymes, Clostridium, Lactobacillus, Petrimonas, Methanoculleus, Prevotella, Methanobacterium, Methanosarcina, Methanothrix, Proteiniphilum, Bellilinea, Anaerolinea, Anaeromassilibacillus, Syntrophomonas and Brevefilum were identified as the key microorganisms in APM and NPM.

Caproiciproducens converts lactic acid into caproic acid during Chinese strong-flavor Baijiu brewing.

Strong-flavor Baijiu, a type of Chinese liquor, is produced through anaerobic solid-state fermentation in a sealed mud pit. Ethyl caproate, the characteristic flavor compound of strong-flavor Baijiu, is influenced by caproic acid-producing bacteria in the pit mud. To better understand the formation of caproic acid, this study investigated the microbial composition and physicochemical parameters of pit mud from different layers (top, middle, and bottom) in Hubei and Sichuan provinces, China. The results revealed that Caproiciproducens plays a key role in caproic acid production by using lactic acid as a substrate, with its abundance increasing with the depth of the pit mud. A strain Caproiciproducens sp. R1, isolated from the pit mud, was shown to produce caproic acid from lactic acid within an initial pH range of 5.5-9.0 and lactic acid concentrations of 1 %-5 % (m/v). In addition, inoculation of strain R1 into Huangshui (a lactic acid-rich liquid from Baijiu production) resulted in 40.72 mM caproic acid production. This study demonstrates that Caproiciproducens plays a crucial role in caproic acid production from lactic acid during the fermentation process of strong-flavor Baijiu.

Optimizing 'Red Fuji' apple quality: Auxin-mediated calcium distribution via fruit-stalk in bagging practices.

In apples, a bottleneck effect in calcium (Ca) transport within fruit stalk has been observed. To elucidate that how auxin affects Ca forms and distribution in the apple fruit stalk, we investigated the effects of different concentrations of auxin treatment (0, 10, 20, and 30 mg·L-1) on Ca content, forms, distribution, and fruit quality during later stages of fruit expansion. The results showed that auxin treatment led to a dramatic reduction in total Ca content in stalk, while an approximately 30 % increase in fruit. Furthermore, auxin treatment effectively enhanced the functionality of xylem vessels in vascular bundles of the stalk in bagged apples. Finally, TOPSIS method was used to assess fruit quality, with treatments ranked as follows: IAA20 > NAA20 > IAA30 > IAA10 > CK > NPA. The findings lay a foundation for further studies on the bottleneck in Ca transport within stalk, uneven distribution of Ca in fruit, and provide insights into Ca utilization efficiency in bagged apples.

Variance investigation on the microstructural characteristics of vessels among three lignocellulosic biomasses.

Especially, the processing and utilization of biomass-based material is closely related to the vessel, e.g. the flow of vapour and additive. It is conventional that vessels in most plants can influence on water and nutrients transport between adjacent cells, which could just infer to be important in the wood-based panel industries. In this work, a complete characterization of vessels and pits is presented for three conventional biomasses in wood-based panel: poplar (Populus deltoides) (P), moso bamboo (Phyllostachys edulis) (B), and the fruit shell of oil camellia (Camellia Oleifera) (FS_OC). Every material is analyzed by combining several techniques including: light microscopy, scanning electron microscopy and surveying calculations from resin casting. The results show that among the three biomass materials, B has a significantly larger vessel width (164.8 ± 6.0 µm for B, 2.2 ± 6.2 µm for P, 10.0 ± 0.8 µm for FS_OC) and smaller inclination angle of the perforation plates (6.8° for B, 44.7° for P), which is more conductive to improving moisture transfer between the vessels. The vessel length of P varies widely from 676.8 µm to 1025.2 µm, which is related to its seasonal growth. By resin casting analysis, more differences in the morphology and distribution of pits in the vessel walls were observed between the three species. Such as, For B, there are numerous pits between vessel cells, while very few to none between vessel and parenchyma cells or fiber. In addition to pits, B and FS_OC also have spiral thickening structures on their vessel walls. The pit membrane is an elliptical shape in P, while slit-like shape in FS_OC and a combination of both elliptical and slit-like shape in bamboo. The unique microstructural characteristics of vessels is related to the individual plant growth traits, which is the basis for biomass-based material processing and utilization.

Near-infrared photoimmunotherapy in cancer treatment: a bibliometric and visual analysis.

Background: Near-infrared photoimmunotherapy (NIR-PIT) is an emerging cancer treatment technology that combines the advantages of optical technology and immunotherapy to provide a highly effective, precise, and low side-effect treatment approach. The aim of this study is to visualize the scientific results and research trends of NIR-PIT based on bibliometric analysis methods. Methods: The Web of Science Core Collection (WoSCC) database was searched in August 2024 for relevant publications in the field of NIR-PIT. Data were analyzed using mainly CiteSpace and R software for bibliometric and visual analysis of the country/region, authors, journals, references and keywords of the publications in the field. Results: A total of 245 publications were retrieved, including articles (n = 173, 70.61%) and reviews (n = 72, 29.39%). The annual and cumulative number of publications increased every year. The highest number of publications was from the United States (149, 60.82%), followed by Japan (70, 28.57%) and China (33, 13.47%). The research institution with the highest number of publications was National Institutes of Health (NIH)-USA (114, 46.53%). Kobayashi H (109) was involved in the highest number of publications, Mitsunaga M (211) was the most frequently cited in total. CANCERS (17) was the most frequently published journal, and NAT MED (220) was the most frequently co-cited journal. The top 10 keywords include near-infrared photoimmunotherapy (166), photodynamic therapy (61), monoclonal antibody (58), in vivo (50), cancer (46), expression (31), breast cancer (27), enhanced permeability (24), antibody (23), growth factor receptor (16). Cluster analysis based on the co-occurrence of keywords resulted in 13 clusters, which identified the current research hotspots and future trends of NIR-PIT in cancer treatment. Conclusion: This study systematically investigated the research hotspots and development trends of NIR-PIT in cancer treatment through bibliometric and visual analysis. As an emerging strategy, the research on the application of NIR-PIT in cancer treatment has significantly increased in recent years, mainly focusing on the targeting, immune activation mechanism, and treatment efficacy in solid tumors has received extensive attention. Future studies may focus on improving the efficacy and safety of NIR-PIT in cancer treatment, as well as developing novel photosensitizers and combination therapeutic regimens, and exploring the efficacy of its application in a wide range of solid tumors, which will provide an important reference and guidance for the application of NIR-PIT in clinical translation.

A spatiotemporal correlation and attention-based model for pipeline deformation prediction in foundation pit engineering.

In foundation pit engineering, the deformation prediction of adjacent pipelines is crucial for construction safety. Existing approaches depend on constitutive models, grey correlation prediction, or traditional feedforward neural networks. Due to the complex hydrological and geological conditions, as well as the nonstationary and nonlinear characteristics of monitoring data, this problem remains a challenge. By formulating the deformation of monitoring points as multivariate time series, a deep learning-based prediction model is proposed, which utilizes the convolutional neural network to extract the spatial dependencies among various monitoring points, and leverages the bi-directional long-short memory unit network to extract temporal features. Notably, an attention mechanism is introduced to adjust the trainable weights of spatial-temporal features extracted in the prediction. The evaluation of a real-world subway project demonstrates that the proposed model has advantages compared with current models, particularly in long-term prediction. It improves the Adjusted R2 index averagely by from 19.4 to 61.6 % compared with existing models. The proposed model also exhibits a decrease in mean absolute error ranging from 51.5 to 70.3 % compared to others. Experiments and analyses verify that the spatial-temporal dependencies in time series and the attention learning for spatial-temporal features can improve the prediction of such engineering problems.

Glass ionomer fissure sealants versus fluoride varnish application on children's behaviour: a randomised controlled trial.

PURPOSE: Glass ionomer fissure sealant (GIS) and fluoride varnish (NaFV) are two preventive interventions applicable to pre-school children. However, their application effects on young children's behaviour are understudied. The study compared the effects of GIS and NaFV applications on dental anxiety (DA), cooperativeness and pain level in pre-school children when applied to primary second molars in outreach settings. METHODS: Four hundred and thirteen children were recruited for the study, out of which 228 were allocated to NaFV group whilstand 185 allocated to the GIS group. One calibrated examiner screened and randomly allocated the children into either group. Another calibrated examiner recorded the children's DA level and cooperativeness with Frankl Behaviour Rating Scale (FBRS) and Venham Behaviour Rating Scale (VBRS) throughout the treatment period. Subjects self-reported their pain levels using Wong-Baker Faces Scale (WBFS). RESULTS: Significant difference in postoperative anxiety was found using VBRS, with children in NaFV group having more positive scores post-treatment (p = 0.016). Only the type of preventive interventions significantly affected postoperative DA and patient cooperation (p = 0.032), whereas no other clinical findings and socio-demographic factors significantly influenced the children's behaviour postoperatively. CONCLUSION: NaFV application is associated with less DA and more cooperative behaviour in pre-school children compared to GIS application although similar pain levels were recorded.

Optic disc pit maculopathy: Covering or filling technique with human amniotic membrane patch to treat the optic disc pit maculopathy?

PURPOSE: To describe two patients affected by optic disk pit-maculopathy ODP-M surgically treated with pars-plana vitrectomy (PPV) and the apposition of a human Amniotic Membrane (hAM) patch. METHODS: Patients underwent a PPV with the apposition of a hAM patch to cover the ODP. In case 1, a 2nd surgery was necessary to push the hAM patch inside the pit, whereas in case 2 the patch was pulled inside the pit following the pressure gradient when infusion was turned on. RESULTS: After surgery the hAM patch filled the ODP and in both cases, during follow-up, there was a progressive reduction of macular OCT changes and a concomitant BCVA improvement. CONCLUSION: Our experience seems to support the idea that the dynamic fluctuation of pressure between intra-ocular and intra-cranial spaces can direct the movement of fluid through the anomalous communication created by the ODP. In this perspective, our cases seem to support the idea that the primary goal of any surgery should be to create a permanent barrier to fluid migration through the ODP to the intra-ocular space, and that hAM can be successfully used to fill the OPD and to create that permanent barrier to achieve the resolution of ODP-M.

Current understanding of the impact of United States military airborne hazards and burn pit exposures on respiratory health.

Millions of United States (U.S.) troops deployed to the Middle East and Southwest Asia were exposed to toxic airborne hazards and/or open-air burn pits. Burn pit emissions contain particulate matter combined with toxic gasses and heavy metals. Ongoing research has demonstrated that exposures to the airborne hazards from military burn pits have profound and lasting health and wellness consequences. Research on the long-term health consequences of exposure to open burn pits has been limited. Work continues to understand the scope of the health impacts and the underlying pathobiology following exposures and to establish care standards. The U.S. Sergeant First Class Heath Robinson Honoring our Promise to Address Comprehensive Toxics (PACT) Act was signed into law August 2022. This act expands the benefits and services to U.S. Veterans exposed to toxicants, requires the Veterans Health Administration to provide toxic exposure screening, and supports increased research, education, and treatment due to toxic occupational exposures. This review highlights the state of the science related to military burn pit exposures research with an emphasis on pulmonary health. Clinical data demonstrate areas of reduced or delayed pulmonary ventilation and lung pathologies such as small airways scarring, diffuse collagen deposition and focal areas of ossification. Identification and characterization of foreign matter deposition in lung tissues are reported, including particulate matter, silica, titanium oxides, and polycyclic aromatic hydrocarbons. These data are consistent with toxic exposures and with the symptoms reported by post-deployment Veterans despite near-normal non-invasive pulmonary evaluations. On-going work toward new methods for non-invasive pulmonary diagnoses and disease monitoring are described. We propose various studies and databases as resources for clinical and health outcomes research. Pre-clinical research using different burn pit modeling approaches are summarized, including oropharyngeal aspiration, intranasal inhalation, and whole-body exposure chamber inhalation. These studies focus on the impacts of specific toxic substances as well as the effects of short-term and sustained insults over time on the pulmonary systems.

Hydrological classification of mine pit lakes using modelling experiments.

With the growing global prevalence of open-pit mining activities, there is an increasing necessity for sustainable mine life cycle plans with an early outlook towards mine closure. A major consideration in mine closure planning is the potential formation of lakes in the mine void and how these "pit lakes" can be managed to minimise risks and, if possible, create benefits. Understanding the long-term interactions between pit lakes, groundwater, and surface water systems is essential for that purpose. While numerous site-specific studies have been undertaken, there have been no studies that aim to provide a general, broadly applicable understanding of how pit lake hydrology relates to geographical context, and no efforts to hydrologically classify pit lakes using geographical criteria. This research employs an integrated generic pit lake water balance model to examine mine pit lake interactions with the surrounding surface water and groundwater. Simulating 243 scenarios, the influence of five input factors (climate, hydraulic conductivity, regional groundwater level, catchment area and pit slope) on pit lake behaviour up to 6000 years beyond the closure of the mine was considered. The assessment focused on four pit lake hydrological attributes once the system reached equilibrium: water level, time to equilibrium, the fraction of days where the pit recharges groundwater, and the fraction of days where there is surface overflow from the lake. All scenarios were assigned to one of five hydrological classes based on the interactions between the pit lake and the surrounding surface water and groundwater. Our findings show that, in many contexts, general data on climate type and subsurface hydraulic conductivity can yield reliable predictions of a pit lake's long-term hydrological classification without having to develop a detailed, site-specific pit lake model. The classification needs improvements in non-arid climates where inter-annual variation in rainfall is pronounced. The pit lake classification is particularly valuable for first-pass risk assessments to determine whether site-specific modelling is required.

Exploring reported population differences in Norway lobster (Nephrops norvegicus) in the Pomo Pits region of the Adriatic Sea using genome-wide markers.

The Norway lobster (Nephrops norvegicus) is one of the most important decapod crustacean seafood species in the Adriatic Sea. Previous research has identified significant differences in growth rates and maturation timing of Nephrops in the Pomo/Jabuka Pits area compared to other subpopulations in Adriatic fishing grounds. Here, we use 1,623 genome-wide single nucleotide polymorphisms (SNPs) to investigate whether the Pomo Pits subpopulation is genetically different from other sites in the Adriatic and neighbouring seas. We found no genetic differentiation among all sampled Adriatic sites, suggesting high gene flow between Pomo Pits Nephrops and those of surrounding areas. We also found genetic homogeneity between the Adriatic sites and single-site samples from the Aegean and Tyrrhenian Seas. However, we detected distinct genetic differentiation between all Mediterranean sites and an Atlantic site in western Scotland, which provides evidence for a phylogenetic break between the Atlantic and the Mediterranean. Our results indicate that Pomo Pits Nephrops are not genetically different from others sampled in the Adriatic and that key biological parameters in Pomo Pits Nephrops could be driven by spatial variation in fishing pressure and/or environmental factors rather than geographic isolation.

Mode I Stress Intensity Factor Solutions for Cracks Emanating from a Semi-Ellipsoidal Pit.

In linear elastic fracture mechanics, the stress intensity factor describes the magnitude of the stress singularity near a crack tip caused by remote stress and is related to the rate of fatigue crack growth. The literature lacks SIF solutions for cracks emanating from a three-dimensional semi-ellipsoidal pit. This study undertakes a comprehensive parametric investigation of the Mode I stress intensity factor (KI) concerning cracks originating from a semi-ellipsoidal pit in a plate. This work utilizes finite element analysis, controlled by Python scripts, to conduct an extensive study on the effect of various pit dimensions and crack lengths on KI. Two cracks in the shape of a circular arc are introduced at the pit mouth perpendicular to the loading direction. The KI values are calculated using the displacement extrapolation method. The effect of normalized geometric parameters pit-depth-to-pit-width (a/2c), pit-depth-to-plate-thickness (a/t), and crack-radius-to-pit-depth (R/a) are investigated. The crack-radius-to-pit-depth (R/a) is found to be the dominating parameter based on correlation analysis. The data obtained from 216 FEA simulations are incorporated into a predictive model using a k-dimensional (k-d) tree and k-Nearest Neighbour (k-NN) algorithm.

Mechanisms of microbial diversity modulation of mineral black clay to achieve ecological restoration of open-pit mine dump.

The harsh climatic conditions and severe scarcity of surface soil present significant challenges to ecological restoration in open-pit mine dumps within China's type II plant cold resistance zone. To address the topsoil shortage, mineral black clay was used to create synthetic soil. This study explored the application of an ecological restoration bacteria (ERB) consortium to accelerate the ecological restoration of synthetic soil-covered areas by enhancing soil ecosystem construction. The results demonstrated that ERB significantly influenced the native bacterial community structure in mixed black clay. Specifically, ERB disrupted the inhibitory effects of the Actinobacterota phylum on the development of native bacterial diversity, leading to an increase in unclassified_o_Solirubrobacterales sp., norank_f_norank_o_norank_c_KD4-96 sp., Sphingomonas sp., Luteitalea sp., norank_f_Vicinamibacteraceae sp., and other aerobic and anaerobic bacteria. These alterations in soil microbial structure directly impacted soil composition and vegetation diversity. The plant diversity survey and metabolomics analysis revealed that the reduction of harmful substances, such as HPED, HODE, and HOME, in black clay soil improved the growth and distribution of Salsola collina Pall. and Medicago sativa L. This increase facilitated the cycling of key nutrients, such as nitrogen (N) and phosphorus (P), and promoted the establishment of symbiotic relationships between plants, microorganisms, and soil. Ultimately, the ecological remediation of the synthetic soil was achieved through the synergistic effects of ERB, which included the degradation of inhibitory soil components, enhanced nutrient consumption by microbiota and plants, and the overall promotion of ecosystem stability in the reclamation area.

Stability analysis of deep foundation pit with a double-row cast-in-place piles and diagonal steel lattice braces under sloped excavation conditions.

Existing deep foundation pit support structures are commonly composed of external earth-retaining structures, internal horizontal bracings, and vertical columns. A closed bracing system, often formed by a horizontal support through a bracket board, frequently impedes vertical excavation and soil removal operations in the foundation pit, and the processes of assembly and dismantling are complex and time-consuming. This study presents a combined support system and construction method consisting of cast-in-place piles and diagonal steel lattice braces. For sloped excavation, diagonal braces were constructed by slotting through the reserved soil, allowing the use of a single layer of support within the excavation depth. This approach significantly optimizes the construction process, reducing both project duration and overall cost. The field monitoring results indicated that the support method effectively controlled the lateral displacement of the pile bodies. Field monitoring results demonstrated that the proposed support system effectively controlled the lateral displacement of the pile bodies. The adoption of a support-first, excavation-second approach significantly controlled the settlement of the ground surface around the foundation pit, thereby preventing excessive increments in the axial force of the supports due to the large longitudinal depth excavation. The calculation results of the three-dimensional finite element model for foundation pit excavation and support indicate that the proposed support method results in a decreasing ratio of the maximum lateral deformation depth of the pile body, denoted as δh-m, to the excavation depth He as the excavation depth increases. This implied that the displacement of the pile body was strictly controlled. When the depth of the foundation pit excavation exceeded 10 m, the maximum lateral deformation occurred below 10 m along the pile shaft. The diagonal steel lattice braces transferred the load to the top of the cast-in-place piles at the bottom of the pit, where the stress concentration occurred. During construction, special attention must be paid to the strength of the connection between the pile top and the connecting beams.

Pilonidal granuloma formation after an incision and drainage procedure is associated with retained hair within the sinus - A case series.

INTRODUCTION AND IMPORTANCE: Pilonidal disease may present with a draining secondary sinus or granuloma, but the development of these findings is not well-characterized. CASE PRESENTATION: Two adolescent males presented with pilonidal disease. The first patient had a gluteal cleft abscess, and an incision and drainage procedure was performed. Although the abscess resolved, the incision site formed a granuloma with intermittent draining wound with granulation tissue. He underwent a pit-picking procedure along with excision of the granuloma. A large amount of hair was also removed from within the pilonidal sinus. The second patient underwent an incision and drainage procedure to treat the pilonidal abscess. The incision site evolved into a granuloma with recurring drainage. A pit-picking procedure was performed, and the granuloma was excised. During the excision, a moderate amount of hair was evacuated from the pilonidal sinus. CLINICAL DISCUSSION: Many pilonidal patients present with a granuloma or secondary sinus at the gluteal cleft, but there has been no documentation of the natural history of this development. The role of hair is central to pilonidal disease pathophysiology and is a known factor in foreign body granuloma formation - a cutaneous inflammatory response to endogenous or exogenous material in the dermis that is not broken down readily by macrophages. CONCLUSION: Even though the pilonidal abscess was drained with an incision, a granuloma was able to form with recurrent drainage when hair was retained within the pilonidal sinus.

Multilayered Inverted Internal Limiting Membrane Flap Technique in Optic Disc Pit Maculopathy.

Objectives: To evaluate the anatomical and visual outcomes of the multilayered inverted internal limiting membrane (ML-ILM) flap technique in the treatment of optic disc pit maculopathy (ODPM). Materials and Methods: In this retrospective interventional case series, medical records and macular spectral-domain optical coherence tomography images of patients who underwent combined pars plana vitrectomy with ML-ILM flap surgery for ODPM were analyzed. Best-corrected visual acuity (BCVA) and central macular thickness (CMT) at postoperative 6 months were compared with baseline findings. Intraoperative and postoperative complications, fluid resolution time, and recurrence during follow-up were recorded. Results: Five eyes of 5 patients with ODPM were included in the study. According to the preoperative macular fluid characteristics, 2 patients had only intraretinal fluid, while 3 patients had intraretinal and subretinal fluid. The preoperative median BCVA was 1.0 logarithm of the minimum angle of resolution (logMAR) (range, 1.0-1.3 logMAR), and the CMT was 560 µm (range, 452-667 µm). At the 6-month postoperative follow-up, the median BCVA was 0.40 logMAR (range, 0.1-0.7 logMAR), and CMT was 315 µm (range, 265-326 µm) (p=0.042 and p=0.043, respectively). During the 6-month follow-up period, no recurrence or full-thickness macular hole formation was observed. Conclusion: The ML-ILM flap technique is a preferable surgical option to achieve both high anatomical and functional success and flap stabilization.

Electrochemical Machining of Micro-Pit Arrays on a GH4169 Alloy with a Roll-Print Mask Using a C6H5Na3O7-Containing NaNO3 Mixed Electrolyte.

GH4169 alloy, a nickel-based superalloy known for its excellent high temperature resistance, corrosion resistance, mechanical properties, and high-temperature tribological properties, is widely used in industrial applications, such as in gas turbines for space shuttles and rocket engines. This study addresses the issue of electrolyte product residue in the electrochemical machining process of a GH4169 alloy by utilizing a C6H5Na3O7-containing NaNO3 new mixed electrolyte. Comparative investigations of the electrochemical behavior and electrolyte product removal mechanisms at different concentrations of C6H5Na3O7 additive in NaNO3 solutions were conducted. The effects of additives, applied voltage, and the rotating speed of the cathode tool on the processing performance of micro-pit arrays on a GH4169 alloy were analyzed. The results indicate that the mixed solution containing C6H5Na3O7 significantly improves the localization and geometric morphology of the micro-pits compared to a single NaNO3 solution. The optimal electrochemical machining parameters were identified as 0.5 wt% C6H5Na3O7 + 10 wt% NaNO3 mixed electrolyte, 12 V applied voltage, and 0.1 r/min rotating speed of the cathode tool. Under these conditions, high-quality micro-pit arrays with an average diameter of 405.85 µm, an average depth of 87.5 µm, and an etch factor (EF) of 1.67 were successfully fabricated, exhibiting excellent morphology, localization, and consistency.

The impact of the door-to-antivenom time on the resolution of coagulopathy caused by green pit viper bite-a retrospective cohort study.

Trimeresurus albolabris (green pit viper) accounts for 95% of human venomous snakebites in Hong Kong and the Green Pit Viper antivenin has become the only antivenom available. Little is known about the impact of early antivenom administration on the duration of venom-induced coagulopathy. This retrospective study aimed to evaluate the impact of the door-to-antivenom time (DTAT) on the duration of such coagulopathy. Consecutive patients with green pit viper bite reported to the Hong Kong Poison Control Centre between 1 January 2012 and 31 December 2022 were included. Electronic medical records were reviewed, and the time and dose of antivenom administration were examined. The level of coagulopathy before and after antivenom was graded using the modified Snakebite Severity Scale. The primary outcome was the duration of venom-induced coagulopathy. Univariate and multivariable generalized linear regression analyses were used to evaluate the association between DTAT and the duration of coagulopathy. In total, 82 adult cases (median age 56 years, 51.2% men) were analyzed. The median DTAT was 4.2 h. DTAT was correlated with the duration of coagulopathy (Spearman r 0.426, p < 0.001), which was correlated with the hospital length of stay (Spearman r 0.357, p = 0.001). However, DTAT was not correlated with the hospital length of stay (Spearman r 0.105, p = 0.346). After adjusting for confounding factors, DTAT (adjusted regression coefficient [ß] 1.73, 95% confidence interval [CI] 0.38 to 3.08, p = 0.012), pre-antivenom level of coagulopathy (adjusted ß 17.08, 95% CI 3.00 to 31.16, p = 0.017), platelet transfusion (adjusted ß 217.11, 95% CI 70.43 to 363.80, p = 0.004), and transfusion of fresh frozen plasma (adjusted ß -175.34, 95% CI 330.90 to -19.77, p = 0.027) were significantly associated with the duration of coagulopathy. These findings suggest that prompt administration of antivenom may shorten the duration of coagulopathy.

Determinants of environmental changes in human-modified ecosystems: Effects of plastics on moisture gradients, nutrients, and clay properties.

Plastic pollution poses a significant threat to ecosystem health worldwide. This study examines the determinants of environmental changes in human-modified ecosystems through a quantitative-qualitative system dynamics modeling approach: field experiments conducted on a 310 m2 unsaturated clay-rich bed and a 2.5 m2 clay-rich shore of a plastic-impacted pond in Shenzhen, China, and a 1.17 ha plastic-impacted clay pit in Musanze, Rwanda; laboratory experiments involving Modified Proctor (MP) and California Bearing Ratio (CBR) tests on natural clay reinforced with polyethylene terephthalate (PET) microplastics, with diameters ranging from 0.25 to 5 mm and at concentrations of 1.25 %, 2.5 %, 3.75 %, 5 %, and 10 % by weight of clay; and plastic dynamic flows analyzed by modeling the life cycle of PET. Field experiments showed that mulch type and thickness were critical factors influencing crack distribution in a plastic-impacted pond bed. Specifically, cracks were dominant in areas with pronounced desiccation and lacking filamentous green algae and PET-dominated plastic waste. Along the 2.5 m moisture gradient in a plastic-impacted pond bed, temperature and moisture significantly influenced nutrients, particularly in pronounced desiccation zones. Laboratory experiments showed that microplastics altered the structural properties of natural clay, decreasing moisture content while increasing dry density and load-bearing capacity. The plastic life cycle underscored the roles of industrial and consumer practices, environmental conditions, and waste management and recycling inefficiencies in driving environmental changes in human-modified ecosystems. The findings underscore the need for effective plastic waste management and recycling to mitigate the ecological impacts of plastic pollution in ecosystems.

Passage efficiency through fishways of species of the family Cyprinidae and their management implications for fragmented rivers.

The contemporary management of fragmented river systems is in a trade-off between the societal benefits of instream barriers (e.g. hydropower, flood risk management) and the ecological harms of their adverse impacts on fish populations. The consequent fragmentation can be mitigated through fishway construction, with mitigation performance measured using species-specific passage rates and efficiencies. There is, however, a bias in passage efficiency studies towards diadromous fishes and, although fish of the Cyprinidae family play a significant role in the fish assemblages of rivers worldwide, their passage efficiencies are poorly understood. Here, systematic review and meta-analyses assessed the passage efficiencies of cyprinid fishes through fishways that have been measured using telemetry methods. Passive integrated transponder (PIT) telemetry was the most common evaluation method of passage efficiency due to their high read rates and relatively low costs versus alternative telemetry methods. These methods revealed cyprinid passage efficiencies were highest through vertical slot fishways and lowest through nature-like constructions, with overall passage rates comparing favourably to anadromous salmonid fishes. Fish were most active during spring and summer, with passage and associated movements often related to spawning. Passage rates of non-native fishes were also higher than for native fishes. Despite the growing acknowledgment of how fishways influence potamodromous fish dispersal and distribution in rivers, passage data remain scarce, preventing managers and policy-makers from making informed decisions on optimal passage solutions for multiple fish species in highly fragmented rivers.