Declining aqueous calcium and fish predation risk interactively modify the phenotypic plasticity in Daphnia pulex.

Aqueous calcium (Ca) decline is threatening freshwater ecosystems worldwide. There are great concerns about the possible ecological consequences of Ca limitation combined with biological pressures like predation. Here we investigated the interactions between Ca restriction and fish predation risk on the phenotypic plasticity in the keystone herbivore Daphnia, together with physiological responses underlying the plastic trait changes. Fish predation risk induced D. pulex to mature earlier and produce more but smaller offspring at adequate Ca. Declining Ca inhibited the expression of defensive traits, with the inhibitive degree showing a linear or threshold-limited dynamic. The presence of predation risk mitigated the negative effect of declining Ca on reducing body size but exacerbated the delay in maturity, indicating a life history trade-off for larger body size rather than the current reproduction in multi-stressed Daphnia. Actin 3-mediated cytoskeleton and AMPK ß-mediated energy metabolism were highly correlated with these plastic trait changes. Altered phenotypic plasticity in planktonic animals is expected to trigger many ecological impacts from individual fitness to community structure, thus providing new insights into the mechanisms underlying decreased Ca affecting lake ecosystems.

Physiological constraints of visual pathway lead to more efficient coding of information in retina.

Nowadays, numerous studies have investigated the modeling of efficient neural encoding processes in the retina of the eye to encode the sensory data. Retina, as the innermost coat of the eye, is the first and the most important area of the visual neural system of mammalians, which is responsible for neural processes. Retina encodes the information of light intensity into a sequence of spikes, and sends them to retinal ganglion cells (RGCs) for further processing. An appropriate retinal encoding model should be adapted to the real retina as much as possible by considering the physiological constraints of the visual pathway to transfer most of the information of the input signal to the brain without too much redundancy of the channel. In this paper, inspired from the existing linear models of retinal encoding process, which have employed input noise and the spatial locality of the RGCs receptive fields (RFs) in the calculation of the encoding matrix, two extra physiological constraints, adapted from the real retina are taken into account so as to achieve a more realistic model for themammalian retina. These new constraints that are the correlation between RGCs and the spatial locality of the photoreceptors' projective fields (PFs), are modeled in a mathematical form and analyzed in detail. To quantify fidelity of the proposed encoding matrix and prove its superiority over existing models, various parameters of the models are calculated and presented in this paper: mean square error between the original and reconstructed image (MSE), the redundancy of the channel, the amount of information transferred through the channel, and the amount of wasted capacity for carrying input noise, to name a few. The results of these calculations show that the proposed model transfers input information with less redundancy of the channel. In other words, it reduces a portion of channel capacity which is wasted for carrying the input noise in comparison to the existing models. Also, due to considering extra physiological constraints in the proposed model, it is acceptable to have a slightly higher amount of MSE value in order to become similar to the real retina.

Androgenic modulation of extraordinary muscle speed creates a performance trade-off with endurance.

Performance trade-offs can dramatically alter an organism's evolutionary trajectory by making certain phenotypic outcomes unattainable. Understanding how these trade-offs arise from an animal's design is therefore an important goal of biology. To explore this topic, we studied how androgenic hormones, which regulate skeletal muscle function, influence performance trade-offs relevant to different components of complex reproductive behaviour. We conducted this work in golden-collared manakins (Manacus vitellinus), a neotropical bird in which males court females by rapidly snapping their wings together above their back. Androgens help mediate this behavior by radically increasing the twitch speed of a dorsal wing muscle (scapulohumeralis caudalis, SH), which actuates the bird's wing-snap. Through hormone manipulations and in situ muscle recordings, we tested how these positive effects on SH speed influence trade-offs with endurance. Indeed, this latter trait impacts the display by shaping signal length. We found that androgen-dependent increases in SH speed incur a cost to endurance, particularly when this muscle performs at its functional limits. Moreover, when behavioural data were overlaid on our muscle recordings, displaying animals appeared to balance display speed with fatigue-induced muscle fusion (physiological tetanus) to generate the fastest possible signal while maintaining an appropriate signal duration. Our results point to androgen action as a functional trigger of trade-offs in sexual performance - these hormones enhance one element of a courtship display, but in doing so, impede another.

Generalized host-plant feeding can hide sterol-specialized foraging behaviors in bee-plant interactions.

Host-plant selection is a key factor driving the ecology and evolution of insects. While the majority of phytophagous insects is highly host specific, generalist behavior is quite widespread among bees and presumably involves physiological adaptations that remain largely unexplored. However, floral visitation patterns suggest that generalist bees do not forage randomly on all available resources. While resource availability and accessibility as well as nectar composition have been widely explored, pollen chemistry could also have an impact on the range of suitable host-plants. This study focuses on particular pollen nutrients that cannot be synthesized de novo by insects but are key compounds of cell membranes and the precursor for molting process: the sterols. We compared the sterol composition of pollen from the main host-plants of three generalist bees: Anthophora plumipes, Colletes cunicularius, and Osmia cornuta, as well as one specialist bee Andrena vaga. We also analyzed the sterols of their brood cell provisions, the tissues of larvae and nonemerged females to determine which sterols are used by the different species. Our results show that sterols are not used accordingly to foraging strategy: Both the specialist species A. vaga and the generalist species C. cunicularius might metabolize a rare C27 sterol, while the two generalist species A. plumipes and O. cornuta might rather use a very common C28 sterol. Our results suggest that shared sterolic compounds among plant species could facilitate the exploitation of multiple host-plants by A. plumipes and O. cornuta whereas the generalist C. cunicularius might be more constrained due to its physiological requirements of a more uncommon dietary sterol. Our findings suggest that a bee displaying a generalist foraging behavior may sometimes hide a sterol-specialized species. This evidence challenges the hypothesis that all generalist free-living bee species are all able to develop on a wide range of different pollen types.

Planning a sports training program using Adaptive Particle Swarm Optimization with emphasis on physiological constraints.

OBJECTIVE: An effective training plan is an important factor in sports training to enhance athletic performance. A poorly considered training plan may result in injury to the athlete, and overtraining. Good training plans normally require expert input, which may have a cost too great for many athletes, particularly amateur athletes. The objectives of this research were to create a practical cycling training plan that substantially improves athletic performance while satisfying essential physiological constraints. Adaptive Particle Swarm Optimization using ɛ-constraint methods were used to formulate such a plan and simulate the likely performance outcomes. The physiological constraints considered in this study were monotony, chronic training load ramp rate and daily training impulse. RESULTS: A comparison of results from our simulations against a training plan from British Cycling, which we used as our standard, showed that our training plan outperformed the benchmark in terms of both athletic performance and satisfying all physiological constraints.

Behavioural effects of temperature on ectothermic animals: unifying thermal physiology and behavioural plasticity.

Temperature imposes significant constraints on ectothermic animals, and these organisms have evolved numerous adaptations to respond to these constraints. While the impacts of temperature on the physiology of ectotherms have been extensively studied, there are currently no frameworks available that outline the multiple and often simultaneous pathways by which temperature can affect behaviour. Drawing from the literature on insects, we propose a unified framework that should apply to all ectothermic animals, generalizing temperature's behavioural effects into: (1) kinetic effects, resulting from temperature's bottom-up constraining influence on metabolism and neurophysiology over a range of timescales (from short to long term), and (2) integrated effects, where the top-down integration of thermal information intentionally initiates or modifies a behaviour (behavioural thermoregulation, thermal orientation, thermosensory behavioural adjustments). We discuss the difficulty in distinguishing adaptive behavioural changes from constraints when observing animals' behavioural responses to temperature. We then propose two complementary approaches to distinguish adaptations from constraints, and categorize behaviours according to our framework: (i) 'kinetic null modelling' of temperature's effects on behaviour; and (ii) behavioural ecology experiments using temperature-insensitive mutants. Our framework should help to guide future research on the complex relationship between temperature and behaviour in ectothermic animals.

A Mountain or a Plateau? Hematological Traits Vary Nonlinearly with Altitude in a Highland Lizard.

High-altitude organisms exhibit hematological adaptations to augment blood transport of oxygen. One common mechanism is through increased values of blood traits such as erythrocyte count, hematocrit, and hemoglobin concentration. However, a positive relationship between altitude and blood traits is not observed in all high-altitude systems. To understand how organisms adapt to high altitudes, it is important to document physiological patterns related to hypoxia gradients from a greater variety of species. Here, we present an extensive hematological description for three populations of Sceloporus grammicus living at 2,500, 3,400, and 4,300 m. We did not find a linear increase with altitude for any of the blood traits we measured. Instead, we found nonlinear relationships between altitude and the blood traits erythrocyte number, erythrocyte size, hematocrit, and hemoglobin concentration. Erythrocyte number and hematocrit leveled off as altitude increased, whereas hemoglobin concentration and erythrocyte size were highest at intermediate altitude. Additionally, lizards from our three study populations are similar in blood pH, serum electrolytes, glucose, and lactate. Given that the highest-altitude population did not show the highest levels of the variables we measured, we suggest these lizards may be using different adaptations to cope with hypoxia than lizards at low or intermediate altitudes. We discuss future directions that research could take to investigate such potential adaptations.

Effect of diet quality and ambient temperature on the use of torpor by two species of neotropical nectar-feeding bats.

Neotropical bats use torpor as a strategy to save energy when they experience a low energy intake and/or low ambient temperature (Ta). Digestive physiology limits the energy intake of several glossophaginid bats, and could play an important role in the onset of torpor in these tropical animals. We measured the effect that diet quality and Ta had on the use of torpor by the nectar-feeding bats Glossophaga soricina and Leptonycteris yerbabuenae Captive bats were fed with 5% (low) or 35% (high) sucrose solutions while exposed to two different Ta (17.7 and 23.2°C; low Ta and high Ta) in four different treatments: (1) high sucrose:high Ta, (2) high sucrose:low Ta, (3) low sucrose:high Ta and (4) low sucrose:low Ta We measured their energy intake, changes in body mass (ΔMb) and skin temperature (Tskin) as response variables. Energy intake (in 10 h) was limited when both species fed on 5% sucrose, but body mass gain was only affected in G. soricina. Energy intake and Ta had a negative effect on the minimum Tskin of both species, and ΔMb affected the time that G. soricina used torpor. Both species remained normothermic on the high sucrose:high Ta treatment, but used torpor on the other three treatments. Bats used torpor during their resting and activity periods. Leptonycteris yerbabuenae spent more time in torpor in the low sucrose:high Ta treatment, while G. soricina used this strategy for longer periods of time in the high sucrose:low Ta treatment. We found that diet quality and Ta played an important role in the use of torpor by nectar-feeding bats.

Coping with shorter days: do phenology shifts constrain aphid fitness?

Climate change can alter the phenology of organisms. It may thus lead seasonal organisms to face different day lengths than in the past, and the fitness consequences of these changes are as yet unclear. To study such effects, we used the pea aphid Acyrthosiphon pisum as a model organism, as it has obligately asexual clones which can be used to study day length effects without eliciting a seasonal response. We recorded life-history traits under short and long days, both with two realistic temperature cycles with means differing by 2 °C. In addition, we measured the population growth of aphids on their host plant Pisum sativum. We show that short days reduce fecundity and the length of the reproductive period of aphids. Nevertheless, this does not translate into differences at the population level because the observed fitness costs only become apparent late in the individual's life. As expected, warm temperature shortens the development time by 0.7 days/°C, leading to faster generation times. We found no interaction of temperature and day length. We conclude that day length changes cause only relatively mild costs, which may not decelerate the increase in pest status due to climate change.