RESUMEN
Terrestrial orchids represent a symbiotic union between plants and mycorrhizal fungi. This study describes the occurrence and nature of viruses associated with one population of wild Pterostylis sanguinea orchids, including their fungal symbionts, over two consecutive years. A generic sequencing approach, which combined dsRNA-enrichment from plant and mycelial tissues, random amplification and high throughput shotgun sequencing was used to identify novel viruses. The majority of the virus-like sequences represent partial genomes, and their identification is based solely on de novo assembly of sequencing data. In orchid leaf tissues we found three isolates of a novel totivirus and an unclassified virus; both resemble fungus-infecting viruses. Two isolates of Ceratobasidium sp that were isolated from orchid underground stems contained at least 20 viruses, 16 of which were previously described as alphapartitiviruses and betapartitiviruses. A novel hypovirus and a mitovirus were genetically distant from existing members of the genera and did not readily fit into recognised subgroups.
Asunto(s)
Virus Fúngicos/genética , Micorrizas/virología , Orchidaceae/microbiología , Virus ARN/genética , Simbiosis/fisiología , Totivirus/genética , Virus no Clasificados/genética , Australia , Virus Fúngicos/clasificación , Virus Fúngicos/aislamiento & purificación , Secuenciación de Nucleótidos de Alto Rendimiento , Filogenia , Hojas de la Planta/microbiología , Virus ARN/clasificación , Virus ARN/aislamiento & purificación , ARN Bicatenario/genética , Análisis de Secuencia de ADN , Totivirus/clasificación , Totivirus/aislamiento & purificación , Virus no Clasificados/clasificación , Virus no Clasificados/aislamiento & purificaciónRESUMEN
The bipartite alpha- and betapartitiviruses are recorded from a wide range of fungi and plants. Using a combination of dsRNA-enrichment, high-throughput shotgun sequencing and informatics, we report the occurrence of multiple new partitiviruses associated with mycorrhizal Ceratobasidium fungi, themselves symbiotically associated with a small wild population of Pterostylis sanguinea orchids in Australia, over two consecutive years. Twenty-one partial or near-complete sequences representing 16 definitive alpha- and betapartitivirus species, and further possible species, were detected from two fungal isolates. The majority of partitiviruses occurred in fungal isolates from both years. Two of the partitiviruses represent phylogenetically divergent forms of Alphapartitivirus, suggesting that they may have evolved under long geographical isolation there. We address the challenge of pairing the two genomic segments of partitiviruses to identify species when multiple partitiviruses co-infect a single host.