Genomic Sequencing to Detect Cross-Breeding Quality in Dogs: An Example Studying Disorders in Sexual Development.

Disorders of sexual development (DSDs) in dogs, similar to humans, arise from genetic mutations, gonadal differentiation, or phenotypic sex development. The French Bulldog, a breed that has seen a surge in popularity and demand, has also shown a marked increase in DSD incidence. This study aims to characterize the genetic underpinnings of DSDs in a French Bulldog named Brutus, exhibiting ambiguous genitalia and internal sexual anatomy, and to explore the impact of breeding practices on genetic diversity within the breed. We utilized a comprehensive approach combining conventional cytogenetics, molecular techniques, and deep sequencing to investigate the genetic profile of Brutus. The sequence data were compared to three other male French Bulldogs' genome sequences with typical reproductive anatomy, including Brutus's father and the canine reference genome (CanFam6). We found a Robertsonian fusion involving chromosome 23 previously reported in dogs as a causative mutation responsible for sex reversal syndrome. Our findings revealed a 22% mosaicism (78,XX/77,XX), the absence of the sex-determining region (SRY) gene, and the presence of 43 unique Single Nucleotide Variants (SNVs) not inherited from the father. Notably, the run of homozygosity (ROH) analysis showed Brutus has a higher number of homozygous segments compared to other Bulldogs, with a total length of these fragments 50% greater than the average, strongly suggesting this dog is the product of the mating between siblings. Although no direct causative genes for the DSD phenotype were identified, four candidate loci warrant further investigation. Our study highlighted the need for a better annotated and curated reference dog genome to define genes causative of any specific phenotype, suggests a potential genetic basis for the DSD phenotype in dogs, and underscores the consequences of uncontrolled breeding practices in French Bulldogs. These findings highlight the importance of implementing strategic genetic management to preserve genetic health and diversity in canine populations.

A Respiratory Sensor-Based Study of the Relationship between Voluntary Breathing Patterns and Aerobic and Anerobic Exercise Capacity-An Exploratory Applied Study.

(1) Background: Exploring the relationship between spontaneous breathing patterns and aerobic and anerobic running exercise performance can greatly improve our understanding of optimizing physical fitness. Spontaneous breathing patterns refer to how the rhythm and depth of breathing affect performance and physical adaptation during exercise. (2) Methods: This study aimed to investigate this relationship by enrolling 240 college students (120 males and 120 females, aged 18-22). We evaluated their resting respiratory rate (RR), the combined total of abdominal and thoracic movements (AM+TM), the proportion of abdominal movement to the overall respiratory movement (AM/(AM+TM)), and the inhalation to exhalation ratio (I/E ratio). Additionally, their performance in a 50 m sprint (measuring anerobic capability), an 800- or 1000 m run (assessing mixed aerobic and anerobic capacity), and a 12 min distance run (evaluating aerobic capacity) was recorded. (3) Results: Our findings, through both correlational and comparative analyses, indicate that a larger AM+TM is predictive of a greater distance covered in the 12 min run, suggesting enhanced aerobic capacity. Interestingly, among female participants, a lower body mass index (BMI) coupled with a higher proportion of abdominal movement (AM/(AM+TM)) was linked to better performance in the 800 m run, indicative of superior mixed aerobic and anerobic capacities. These results imply that women with a larger tidal volume and those with a lower BMI but higher abdominal contribution to breathing at rest may exhibit better aerobic and mixed exercise capacities, respectively. (4) Conclusions: Based on these findings, we recommend that healthcare professionals and physical education instructors incorporate respiratory pattern assessments into their practices to potentially improve the physical health of their clients and students, with a particular emphasis on female populations. This study underscores the importance of understanding the intricate relationship between spontaneous respiratory patterns and exercise capabilities in enhancing overall physical fitness and health.

Ruin-of-the-rivers? A global review of run-of-the-river dams.

The classification of a hydropower scheme as run-of-the-river (or run-of-river; ROR) evokes an image of a low-impact installation; however, examination of eight case studies worldwide shows that substantial negative societal and ecological impacts are tied to them, albeit in somewhat different ways. We conclude that ROR dams not only potentially displace communities, disrupt livelihoods, and degrade environments in surrounding areas, but they also divert water from areas of need, impact aquatic ecology through habitat destruction and disruption of fish migrations, emit non-trivial amounts of greenhouse gases over the lifespan of the project, and disrupt streamflow in downstream river sections. While these negative impacts vary on a case-by-case basis, medium and large ROR dams consistently have multiple and cumulative impacts, even when not having appreciable reservoirs. We contend that many impactful dams do not qualify as low-impact ROR projects, despite being defined as such. Such mislabeling is facilitated in part by the ambiguous definition of the term, which risks the ROR concept being used by proponents of impactful structures to downplay their negative effects and thus mislead the public or gain status, including within the Clean Development Mechanism in relation to mitigating climate change.

Natural sorting of sediments in the wave run-up zone works for microplastics as well.

The distribution of plastic pollution in the marine environment is highly variable in time and space, making it difficult to assess pollution levels. This study shows that mixing and natural sorting of material in the wave run-up zone of a sandy beach results in a relatively stable abundance of microplastics in the size range 0.5-2 mm (S-MPs). Based on 175 samples collected over 14 months during 42 monitoring surveys at 6 stations along the shore of the Vistula Spit (Baltic Sea), the mean abundance of S-MPs was found to be 64 ± 36 items/kg DW (98.6 % fibers), with a coefficient of variation of only 56 % over more than one year. Statistical tests confirmed its independence from current wind speed, significant wave height, mean sediment grain size, sediment sorting, percentage of certain sand fractions, month, season, or location along the shore. It can therefore be used as a suitable indicator for long-term monitoring of increasing plastic pollution in the marine environment.

Identifying the reciprocal causation between hit-and-run behavior and crash injury severity.

OBJECTIVE: Hit-and-run behavior is believed to exacerbate the injury severity of traffic crashes due to the delayed emergency response for the victims. However, several previous studies indicated the opposite finding that hit-and-run crashes were associated with less severe injuries. The relevant studies mainly identified the statistical associations between hit-and-run behavior and injury severity without revealing causation between them. To this end, the study aims to explore the reciprocal causation between the two variables. METHOD: The two-stage probit model with endogenous regressors is employed to identify the reciprocal causation between hit-and-run behavior and crash injury severity for single- and two-vehicle crashes, respectively, with the use of crash data extracted from the Crash Report Sampling System and Fatality Analysis Reporting System (2016-2019). RESULTS: The results indicate that 1) for both single- and two-vehicle crashes, the fleeing behavior can significantly increase the injury severity of the victims in the crashes while the severe injury of the victims has a negative impact on the propensity of such behavior, 2) the propensity of hit-and-run behavior is influenced by various instrumental variables such as driver age, gender, alcohol involvement, weekday, area type, and light condition, and 3) crash injury severity is significantly related to the victim age, gender, and vehicle damage. CONCLUSIONS: There is a reciprocal causation between hit-and-run behavior and injury severity in traffic crashes. The analytical results can provide a reasonable explanation for the counterintuitive finding on hit-an-run crashes and help mitigate the injury severity.

Specializing When It Counts: Comparing the Dose-Time Effect of Distance Variety between Swimming and Track Running.

OBJECTIVE: To conduct a longitudinal retrospective analysis, explore the relationship between success at peak performance age and the number of different race distances athletes competed in each year (within-sport distance variety), and compare the dose-time effect of this distance variety throughout the development process between male swimmers and track runners. METHODOLOGY: Male swimmers (n = 6033) and track runners (n = 19,278) still competing at peak performance age were ranked, and the number of different race distances was extracted retrospectively for each year until early junior age (13-14-year-old category) from the databases of the European Aquatics and World Athletics federations. Firstly, correlation analysis determined the relationship between ranking at peak performance age and distance variety. Secondly, Poisson distribution provided the probability and dose-time effect of distance variety for becoming an international-class athlete at peak performance age. RESULTS: Generally, correlation analysis revealed low coefficients (r ≤ 0.22) but significant effects (p < 0.001) for larger distance variety and success at peak performance age. Poisson distribution revealed the highest probability of becoming an international-class swimmer when competing in 2-4 race distances at junior age, depending on the primary race distance. The dose-time effect indicated a gradual reduction in the number of race distances as athletes approached peak performance age, narrowing down to 1-2, 2-3, and 3-4 distances for sprint, middle-, and long-distance races, respectively. Track runners exhibited a lower distance variety than swimmers, with a consistent optimum of 1-2 race distances across the age groups. CONCLUSIONS: The present findings including data of the most combined race distances for each primary race distance and a comparison between swimming and track running provide new background information to challenge traditional training regimes and help establish new strategies for long-term athlete development.

Exploring the "led" in health professional student-led experiences: a scoping review.

To support a complex health system, students are expected to be competent leaders as well as competent clinicians. Intentional student leadership development is needed in health professional education programs. Student-led experiences such as student-run clinics and interprofessional training wards, are practice-based learning opportunities where learners provide leadership to clinical services and/or address a gap in the system. Given the absence of leadership definitions and concepts, this scoping review explored how student leadership is conceptualized and developed in student-led experiences. The review was conducted in accordance with best practices in scoping review methodology within the scope of relevant practice-based student-led experiences for health professional students. The research team screened 4659 abstracts, identified 315 articles for full-text review and selected 75 articles for data extraction and analysis. A thematic analysis produced themes related to leadership concepts/theories/models, objectives, facilitation/supervision, assessment and evaluation of curriculum. While responding to system gaps within health professional care, student-led experiences need to align explicit leadership theory/concepts/models with curricular objectives, pedagogy, and assessments to support health professional education. To support future student-led experiences, authors mapped five leadership student role profiles that were associated with student-led models and could be constructively aligned with theory and concepts. In addition to leveraging a student workforce to address system needs, student-led experiences must also be a force for learning through a reciprocal model of leadership and service to develop future health professionals and leaders.

Otolaryngology Diagnoses at a Student-Run Community Clinic.

Purpose Compared to insured individuals who have established pathways for primary and specialist care, uninsured patients often delay seeking medical attention and are more likely to rely on emergency departments or community clinics for low-cost services. This study aims to identify the most prevalent otolaryngology (ENT) diagnoses at a free, student-run community clinic for uninsured patients. Additionally, it seeks to elucidate their management needs in order to inform strategies for enhancing support and care for this vulnerable population. Methods The electronic medical records for a free, student-run community clinic (St. Vincent's Hope Student Clinic in Galveston, Texas) were reviewed. Patient age, sex, chief complaint, date of medical encounter, and encounter diagnoses from April 2021 to May 2024 were recorded; medical diagnoses were then manually stratified into system categories and diagnostic subgroups. Diagnoses unrelated to ENT were excluded and likely represented secondary encounter diagnoses. Results A total of 38 patients were evaluated across 51 total ENT encounters. The average number of encounters per patient per year was 1.34 (1.05-1.63, 95% CI). The most common ENT category was throat (37%), and the most common individual diagnosis was balance problems (11% of total diagnoses). Conclusion The most common ENT system was the throat, followed by ear, nose, and other. Balance problems, reflux, and allergic rhinitis were the most common individual ENT diagnoses. The analysis of data collected for this study may help direct future care and resource allocation at this clinic.

Muscle synergies during the walk-run and run-walk transitions.

Background: Muscular synergies could represent the patterns of muscular activation used by the central nervous system (CNS) to simplify the production of movement. Studies in walking-running transitions described up to nine synergy modules, and an earlier activation of flexor and extension ankle muscular groups compared to running or walking. Our project aims to study the behaviour of muscle synergies in different stance and swing variations of walking-running (WRT) and running-walking (RWT) transitions. Methods: Twenty-four trained men participated in this study. A variable speed protocol on a treadmill was developed to record the activity of 14 muscle during walking, running and relative transitions. The protocol was based on five ramps of 50 seconds each around ± 10 and 20% of the WRT speed. WRT and RWT were identified according to an abrupt change of the duty factor. Analysing surface electromyography using non-negative matrix factorization (NMF) we obtained synergy modules and temporal activation profiles. Alpha threshold for statistical tests set at 0.05. Results: We described four different transition strides, two for increasing speed transitions, and two for decreasing speed transitions. Four to six synergy modules were found in each condition. According to the maximum cosine similarity results, the two identified WRT conditions shared five modules, while the two RWT conditions shared four modules. WRT and RWT overall shared 4.33 ± 0.58 modules. The activation profiles and centres of activation revealed differences among conditions. Discussion: Transition occurred at step level, and transition strides were composed by walk-like and run-like steps. Compared with previous studies in running and walking, both transitions needed earlier activation of a comparable number of synergy modules. Synergies were affected by acceleration: during RWT the need to dissipate energy, to decrease the speed, was achieved by increasing the number of co-activating muscles. This was reflected in fewer synergy modules and different activation profiles compared to WRT. We believe that our results could be enforced in different applied fields, like clinical gait analysis, physiotherapy and rehabilitation, where plans including co-activation of specific muscular groups could be useful. Gait transitions are common in different sports, and therefore also application in training and sport science would be possible.

Effects of the Amyotrophic Lateral Sclerosis-related Q108P Mutation on the Structural Ensemble Characteristics of CHCHD10.

INTRODUCTION: The Q108P pathological variant of the mitochondrial Coiled-Coil-Helix-- Coiled-Coil-Helix Domain-Containing Protein 10 (CHCHD10) has been implicated in amyotrophic lateral sclerosis (ALS). Both the wild-type and CHCHD10Q108P proteins exhibit intrinsically disordered regions, posing challenges for structural studies with conventional experimental tools. METHOD: This study presents the foundational characterization of the structural features of CHCHD10Q108P and compares them with those of the wild-type counterpart. We conducted multiple run molecular dynamics simulations and bioinformatics analyses. RESULT: Our findings reveal distinct differences in structural properties, free energy surfaces, and the outputs of principal component analysis between these two proteins. These results contribute significantly to the comprehension of CHCHD10 and its Q108P variant in terms of pathology, biochemistry, and structural biology. CONCLUSION: The reported structural properties hold promise for informing the development of more effective treatments for ALS.

Run-up velocity and jumping ground reaction force of Chinese female gymnasts.

The run-up velocity and jumping ground reaction force directly influence gymnasts' ability to perform more difficult manoeuvres and win competitions on vault. However, the variables influencing them among elite female gymnasts remain inadequately understood and warrant detailed investigation. This study aimed to analyse critical variables including run-up velocity and jumping ground reaction force, and to compare these factors across different vault types while further exploring the correlations among them. The study hypothesized that there are significant differences in run-up velocity and jumping ground reaction force across vaulting types, and that these differences will affect athletes' performance. To test this hypothesis, the study analyzed data from performance tests on 16 elite Chinese female gymnasts, focusing on vaulting run-up velocity and jumping ground reaction force. Descriptive statistics were used to summarize the data, and binary logistic regression analysis was employed to investigate the relationships between the variables. The study found significant differences in interval run-up velocities between the Front handspring vaults and the Tsukahara vaults, particularly before the last 10 m (p = 0.04) and the last 5 m (p = 0.03). Specifically, the Front handspring vaults had higher velocities before the last 10 m, while the Tsukahara vaults had higher velocities within the last 5 m. Differences were also noted in the lengths of the penultimate (p = 0.03), last (p = 0.01), and hurdle steps (p = 0.04). The jumping ground reaction force showed a strong negative correlation with run-up velocity before the last 10 m (ρ = -0.469, p < 0.01) and the last 5 m (ρ = - 0.604, p < 0.01). However, no significant associations were found with the 25 m run-up velocity (p = 0.346). Significant differences were observed in the hip joint angle (p = 0.001) and the trunk-to-ground angle (p = 0.001), but not in the landing angle (p = 0.435) and take-off angle (p = 0.594) during the take-off phase. These findings suggest that revising the run-up rhythm and improving pedaling techniques could contribute to the successful performance of more difficult vaulting maneuvers for female elite gymnasts in China.

Selected somatic parameters and body composition as predictors of cardiorespiratory fitness among Polish adolescents aged 11-14.

The aim of the study was to verify whether selected somatic parameters and components of body composition were significant predictors of cardiorespiratory fitness (CRF) among a potentially healthy Polish population of adolescents aged 11-14 years. The cross-sectional study was conducted on a group of 375 subjects (164 girls, and 211 boys). A 20 m shuttle run test (20 m SRT) was used to assess CRF. The total number of rounds was taken into account. Basic somatic parameters were measured: body mass (BM), body height (BH), waist circumference (WC), hip circumference (HC), body mass index (BMI), waist-to-hip ratio (WHR) and waist-to-height ratio (WHtR), and body composition components: body fat percentage (FM%), fat mass (FM kg), total body water (TBW), fat-free mass (FFM). Statistical analyses included basic statistical measures (mean and standard deviation) and Spearman rank correlation coefficient. Multiple linear regression analysis was performed to detect significant predictors of CRF. In each proposed model, the dependent variable was the number of rounds, and the independent variables were selected somatic parameters and components of body composition. More than half (65%) of the subjects had an average or lower level of CRF, and 35% of the population presented a good or above good level of CRF. The study showed a statistically significant negative correlation between BM, FM%, FM kg, HC, WC, BMI, WHR, WHtR and the number of laps in the total sample. The strongest correlation in the group of girls was noted for age (r = 0.34) and in the group of boys for FM% (r = - 0.52 ). Each regression model presented proved to be statistically significant, and the significant predictors of CRF in the group of girls were age ( R 2 = 16%) and FM% ( R 2 = 6%). In the group of boys, the significant predictors of CRF were WHtR ( R 2 = 8%) and age ( R 2 = 2%). Estimating body fat distribution is useful in assessing cardiorespiratory fitness, and this in turn indicates its usefulness in preventive screening of school-aged adolescents.

New observations on rare and threatened saproxylic hoverflies in North-Western Italy with ecological notes.

Background: The knowledge on the distribution of saproxylic hoverflies in the Piedmont region has always been incomplete, despite the large wooded areas present in the territory. We know of a few scattered specimens collected in the 19th century, often with a generic or incomplete locality on the label such as "surrounding Turin" or "Alps". The recent publication of the first European Red List on hoverflies highlights some threatened species including some saproxylics. To fill this knowledge gap we report here unpublished data and establish new ecological note on threatened or nationally scarce saproxylic species actually present in the region. New information: In this study, we present new findings concerning rare saproxylic hoverflies. These include the rediscovery of Hammerschmidtiaferruginea (Fallen), 1817, marking its first recorded occurrence in the Italian fauna since the middle of 19th century. Additionally, we report novel observations of the "Chimera fly" Dorosdestillatorius Mik, 1885, along with further observations on endangered or vulnerable species such as Callicera spp. and Brachypalpuschrysites Egger 1859. Moreover, we document the rediscovery of Criorhinapachymera Egger, 1858, within the Piedmont region, and the first record of Brachyopagrunewaldensis Kassebeer, 2000, within Italy. Comprehensive data detailing these observations are provided herein.

Adolescents' health literacy is directly associated with their physical activity but indirectly with their body composition and cardiorespiratory fitness: mediation analysis of the Slovak HBSC study data.

BACKGROUND: Health literacy is a core public health issue in relation to children and adolescents associated with multiple health behaviours and health outcomes. The aim of the study is to test the direct associations between health literacy, physical activity behaviour, health outcomes of body composition and cardiorespiratory fitness among Slovak adolescents and possible indirect effect of health literacy on health outcomes of body composition and cardiorespiratory fitness mediated by adolescents' physical activity behaviour. METHODS: Data from the Slovak Health Behaviour in School-aged Children (HBSC) study conducted in 2022 were used. For the purposes of this study, a subsample of the adolescents (n = 508; mean age = 14.50; SD = 0.82; 54.3% boys) which provided HBSC questionnaire data on health literacy, moderate-to-vigorous physical activity and vigorous physical activity and participated in body composition (InBody 230) and cardiorespiratory fitness (20-m shuttle run test) measurements. Data were analysed using linear regression analysis. RESULTS: The findings showed that higher health literacy of the adolescents was directly associated with higher frequency of physical activity represented by moderate-to-vigorous physical activity and vigorous physical activity and only with the visceral fat area in the crude model. Furthermore, there was an indirect effect of health literacy on cardiorespiratory fitness and most of the body composition variables (except the Body Mass Index) which was mediated by physical activity of the respondents. CONCLUSIONS: Health literacy is indirectly associated to body composition and cardiorespiratory fitness through higher frequency of physical activity. It seems that health literacy as cognitive and social competencies need behavioural components to be involved in the proposed causal pathway between health literacy and health outcomes. Our findings may contribute to the process of creating a framework for future health literacy interventions in adolescents.

Investigating Runner's High: Changes in Mood and Endocannabinoid Concentrations after a 60 min Outdoor Run Considering Sex, Running Frequency, and Age.

Runner's high is a euphoric emotional state occurring during and post-physical exercise. Although previous data indicate endocannabinoids' involvement in animal runner's high, their role in human runner's high remains to be established. We investigated runner's high in healthy humans assessing mood and plasma endocannabinoid concentration changes pre- and post a 60 min outdoor run, considering sex (8 females/8 males), running frequency (4 occasional/12 regular runners) and age (median split 36 years). Mood, AEA, and 2-AG concentrations were significantly increased post-run considering all participants (p < 0.0001, p < 0.0001, p < 0.01, respectively), with 2-AG varying more than AEA concentrations. Concentrations of both endocannabinoids increased pre- to post-run in women (p < 0.01) but the AEA concentration increase was higher in females than in males (p < 0.05). Post-run concentration increase appeared to be more pronounced in occasional than in regular runners for 2-AG but not for AEA. However, regular runners experienced stronger mood increases and better post-run mood than occasional runners. Post-run endocannabinoid concentrations were increased regardless of age. AEA concentrations and their post-run changes were less affected by running frequency and age than those of 2-AG. These findings provide insights into the interplay of physical exercise, physiological/psychological factors and demographics, laying a valuable foundation for future research.

Improved motor imagery training for subject's self-modulation in EEG-based brain-computer interface.

For the electroencephalogram- (EEG-) based motor imagery (MI) brain-computer interface (BCI) system, more attention has been paid to the advanced machine learning algorithms rather than the effective MI training protocols over past two decades. However, it is crucial to assist the subjects in modulating their active brains to fulfill the endogenous MI tasks during the calibration process, which will facilitate signal processing using various machine learning algorithms. Therefore, we propose a trial-feedback paradigm to improve MI training and introduce a non-feedback paradigm for comparison. Each paradigm corresponds to one session. Two paradigms are applied to the calibration runs of corresponding sessions. And their effectiveness is verified in the subsequent testing runs of respective sessions. Different from the non-feedback paradigm, the trial-feedback paradigm presents a topographic map and its qualitative evaluation in real time after each MI training trial, so the subjects can timely realize whether the current trial successfully induces the event-related desynchronization/event-related synchronization (ERD/ERS) phenomenon, and then they can adjust their brain rhythm in the next MI trial. Moreover, after each calibration run of the trial-feedback session, a feature distribution is visualized and quantified to show the subjects' abilities to distinguish different MI tasks and promote their self-modulation in the next calibration run. Additionally, if the subjects feel distracted during the training processes of the non-feedback and trial-feedback sessions, they can execute the blinking movement which will be captured by the electrooculogram (EOG) signals, and the corresponding MI training trial will be abandoned. Ten healthy participants sequentially performed the non-feedback and trial-feedback sessions on the different days. The experiment results showed that the trial-feedback session had better spatial filter visualization, more beneficiaries, higher average off-line and on-line classification accuracies than the non-feedback session, suggesting the trial-feedback paradigm's usefulness in subject's self-modulation and good ability to perform MI tasks.

Genomic and Conventional Inbreeding Coefficient Estimation Using Different Estimator Models in Korean Duroc, Landrace, and Yorkshire Breeds Using 70K Porcine SNP BeadChip.

The purpose of this study was to estimate the homozygosity distribution and compute genomic and conventional inbreeding coefficients in three genetically diverse pig breed populations. The genomic and pedigree data of Duroc (1586), Landrace (2256), and Yorkshire (3646) were analyzed. We estimated and compared various genomic and pedigree inbreeding coefficients using different models and approaches. A total of 709,384 ROH segments in Duroc, 816,898 in Landrace, and 1,401,781 in Yorkshire, with average lengths of 53.59 Mb, 56.21 Mb, and 53.46 Mb, respectively, were identified. Relatively, the Yorkshire breed had the shortest ROH segments, whereas the Landrace breed had the longest mean ROH segments. Sus scrofa chromosome 1 (SSC1) had the highest chromosomal coverage by ROH across all breeds. Across breeds, an absolute correlation (1.0) was seen between FROH total and FROH1-2Mb, showing that short ROH were the primary contributors to overall FROH values. The overall association between genomic and conventional inbreeding was weak, with values ranging from 0.058 to 0.140. In contrast, total genomic inbreeding (FROH) and ROH classes showed a strong association, ranging from 0.663 to 1.00, across the genotypes. The results of genomic and conventional inbreeding estimates improve our understanding of the genetic diversity among genotypes.

Is the Social Safety Net a Long-Term Investment? Large-Scale Evidence From the Food Stamps Program.

We use novel, large-scale data on 17.5 million Americans to study how a policy-driven increase in economic resources affects children's long-term outcomes. Using the 2000 Census and 2001-13 American Community Survey linked to the Social Security Administration's NUMIDENT, we leverage the county-level rollout of the Food Stamps program between 1961 and 1975. We find that children with access to greater economic resources before age five have better outcomes as adults. The treatment-on-the-treated effects show a 6% of a standard deviation improvement in human capital, 3% of a standard deviation increase in economic self-sufficiency, 8% of a standard deviation increase in the quality of neighbourhood of residence, a 1.2-year increase in life expectancy, and a 0.5 percentage-point decrease in likelihood of being incarcerated. These estimates suggest that Food Stamps' transfer of resources to families is a highly cost-effective investment in young children, yielding a marginal value of public funds of approximately sixty-two.

Consuming Beetroot Juice Improves Slalom Performance and Reduces Muscle Soreness in Alpine Skiers under Hypoxic Conditions.

Background: Beetroot juice (BRJ) supplementation has been shown to increase sports performance under hypoxic conditions and to improve athletes' recovery. Objectives: In the present study, we aimed to investigate the effect of acute BRJ supplementation on slalom (SL) run performance and muscle soreness (MS) in Alpine skiers at moderate to high altitudes. Methods: Ten male Alpine skiers received 220 mL of BRJ (8.9 mmol/L nitrate) or placebo (PLA) in 2 sessions with a 7-d wash out interval in a randomized, crossover, PLA-controlled, double-blind study. The 90-s box jump (BJ90), agility hexagonal obstacle jump (Hex Jump), and wall-sit tests were measured before on-hill SL runs in both sessions. After the functional tests, SL run performance was measured by time to complete 2 runs on the SL course; immediately after each SL run, the rating of perceived exertion (RPE) was recorded. In addition, perceived MS was recorded using the visual analog scale at 12, 24, and 48 h after the SL runs. Results: The data were meticulously analyzed using 2-way repeated measures analysis of variance and paired t tests with significance set at P < 0.05. The findings were significant, indicating that compared with PLA, BRJ notably improved wall-sit and BJ90 performances (P < 0.05), while a substantial reduction was observed in RPE, Hex Jump, and MS (P < 0.05). A 1.74% shorter time to complete SL runs was observed in the BRJ group compared with the PLA group; however, there were no significant differences between the PLA and BRJ groups (P > 0.05). Conclusions: These results underscore the potential of BRJ supplementation to enhance sports performance and reduce MS in Alpine skiers under hypoxic conditions.

The Patient Population of a No-Cost, Student-Run LGBTQ+ Mental Health Clinic: A Case for Equitable and Trauma-Informed Care.

Introduction: LGBTQ+ individuals experience disproportionately high rates of mental health disorders. Subpopulations of this community experience unique risk factors and barriers to accessing care. Method: This study analyzes chart review data of patients (n=49) of an LGBTQ+-specific, student-run, free mental health clinic in NYC between March 2019 and July 2021. Result: Most common diagnoses were mood disorders (55%) and anxiety disorders (53%). 88% of patients reported experiencing lifetime traumatic events; 20% of patients met criteria for PTSD. Conclusion: Further research is needed to characterize vulnerable subpopulations to create equitable, accessible, and competent mental health care resources for the LGBTQ+ community.