Anaerobic ammonium oxidation coupled with sulfate reduction links nitrogen with sulfur cycle.

Sulfate-dependent ammonium oxidation (Sulfammox) is a critical process linking nitrogen and sulfur cycles. However, the metabolic pathway of microbes driven Sulfammox is still in suspense. The study demonstrated that ammonium was not consumed with sulfate as the sole electron acceptor during long-term enrichment, probably due to inhibition from sulfide accumulation, while ammonium was removed at âˆ¼ 10 mg N/L/d with sulfate and nitrate as electron acceptors. Ammonium and sulfate were converted into nitrogen gas, sulfide, and elemental sulfur. Sulfammox was mainly performed by Candidatus Brocadia sapporoensis and Candidatus Brocadia fulgida, both of which encoded ammonium oxidation pathway and dissimilatory sulfate reduction pathway. Not sulfide-driven autotrophic denitrifiers but Candidatus Kuenenia stuttgartiensis converted nitrate to nitrite with sulfide. The results of this study reveal the specialized metabolism of Sulfammox bacteria (Candidatus Brocadia sapporoensis and Candidatus Brocadia fulgida) and provide insight into microbial relationships during the nitrogen and sulfur cycles.

Potential electron acceptors for ammonium oxidation in wastewater treatment system under anoxic condition: A review.

Anaerobic ammonium oxidation has been considered as an environmental-friendly and energy-efficient biological nitrogen removal (BNR) technology. Recently, new reaction pathway for ammonium oxidation under anaerobic condition had been discovered. In addition to nitrite, iron trivalent, sulfate, manganese and electrons from electrode might be potential electron acceptors for ammonium oxidation, which can be coupled to traditional BNR process for wastewater treatment. In this paper, the pathway and mechanism for ammonium oxidation with various electron acceptors under anaerobic condition is studied comprehensively, and the research progress of potentially functional microbes is summarized. The potential application of various electron acceptors for ammonium oxidation in wastewater is addressed, and the N2O emission during nitrogen removal is also discussed, which was important greenhouse gas for global climate change. The problems remained unclear for ammonium oxidation by multi-electron acceptors and potential interactions are also discussed in this review.

Ferric and sulfate coupled ammonium oxidation enhanced nitrogen removal in two-stage partial nitrification - Anammox/denitrification process for food waste liquid digestate treatment.

Liquid digestate of food waste is an ammonium-, ferric- and sulfate-laden leachate produced during digestate dewatering, where the carbon source is insufficient for nitrogen removal. A two-stage partial nitrification-anammox/denitrification process was established for nitrogen removal of liquid digestate without pre-treatment (>300 d), through which nitrogen (95 %), biodegradable organics (100 %), sulfate (78 %) and iron (100 %) were efficiently removed. Additional ammonium conversion (20 %N) might be coupled with ferric and sulfate reduction, while produced nitrite could be further converted to di-nitrogen gas through anammox (75 %) and denitrification (25 %). Notably, since increasingly contribution of hydroxylamine producing nitrous oxide, and up-regulated expression of electron transfer and cytochrome c protein, the enhanced ammonium oxidation was probably conducted through extracellular polymeric substances-mediated electron transfer between sulfate/ferric-reducers and aerobic ammonium oxidizers. Thus, the established partial nitrification-anammox/denitrification process might be a cost-efficient nitrogen removal technology for liquid digestate, benefitting to domestic waste recycling and carbon neutralization.

Simultaneous nitrogen and sulfur removal through synergy of sulfammox, anammox and sulfur-driven autotrophic denitrification in a modified bioreactor enhanced by activated carbon.

Anaerobic ammonium (NH4+ - N) oxidation coupled with sulfate (SO42-) reduction (sulfammox) is a new pathway for the autotrophic removal of nitrogen and sulfur from wastewater. Sulfammox was achieved in a modified up-flow anaerobic bioreactor filled with granular activated carbon. After 70 days of operation, the NH4+ - N removal efficiency almost reached 70%, with activated carbon adsorption and biological reaction accounting for 26% and 74%, respectively. Ammonium hydrosulfide (NH4SH) was found in sulfammox by X-ray diffraction analysis for the first time, which confirmed that hydrogen sulfide (H2S) was one of the sulfammox products. Microbial results indicated that NH4+ - N oxidation and SO42- reduction in sulfammox were carried out by Crenothrix and Desulfobacterota, respectively, in which activated carbon may operate as electron shuttle. In the 15NH4+ labeled experiment, 30N2 were produced at a rate of 34.14 µmol/(g sludge·h) and no 30N2 was detected in the chemical control group, proving that sulfammox was present and could only be induced by microorganisms. The 15NO3- labeled group produced 30N2 at a rate of 88.77 µmol/(g sludge·h), demonstrating the presence of sulfur-driven autotrophic denitrification. In the adding 14NH4+ and 15NO3- group, it was confirmed that NH4+ - N was removed by the synergy of sulfammox, anammox and sulfur-driven autotrophic denitrification, where the main product of sulfammox was nitrite (NO2-) and anammox was the main cause of nitrogen loss. The findings showed that SO42- as a non-polluting species to environment may substitute NO2- to create a new "anammox" process.

Sulfate-reducing ammonium oxidation: A promising novel process for nitrogen and sulfur removal.

Sulfate-reducing ammonium oxidation (sulfammox), a novel and promising process that has emerged in recent years, is essential to nitrogen and sulfur cycles and offers significant potential for the elimination of ammonium and sulfate. This review discussed the development of sulfammox process, the mechanism, characteristics of microbes, potential influencing factors, applicable bioreactors, and proposed the research needs and future perspective. The sulfammox process could be affected by many factors, such as the NH4+/SO42- ratio, carbon source, pH, and temperature. However, these potential influencing factors were only obtained based on what has been seen in papers studying related processes such as denitrification, sulfate-reduction, etc., and have to be further tested in bioreactors carrying out the sulfammox process in the future. Currently, sulfammox is predominantly used in granular activated carbon anaerobic fluidized beds, up-flow anaerobic sludge blanket reactors, anaerobic expanded granular bed reactors, rotating biological contact reactors, and moving bed biofilm reactors. In the future, the operating parameters of sulfammox should be further optimized to improve the processing performance, and the system can be further scaled up for actual wastewater treatment. In addition, the isolation, identification, and characterization of key functional microbes and the analysis of microbial interrelationships will also be focused on in future studies to enable an in-depth analysis of the sulfammox mechanism.

Sulfammox forwarding thiosulfate-driven denitrification and anammox process for nitrogen removal.

The coupled process of thiosulfate-driven denitrification (NO3-→NO2-) and Anammox (TDDA) was a promising process for the treatment of wastewater containing NH4+-N and NO3--N. However, the high concentration of SO42- production limited its application, which needs to be alleviated by an economical and effective way to promote the application of TDDA process. In this study, TDDA process was started in a relatively short time by stepwise replacing nitrite with nitrate and operated continuously for 146 days. Results presented that the average total nitrogen removal efficiency of 82.18% can be acquired at a high loading rate of 1.98 kg N/(m3·d) with maximum nitrogen removal efficiency up to 87.04%. It was observed that the increase of S/N ratio improved the denitrification efficiency and slightly inhibit the Anammox process. Batch tests showed that Sulfammox process appeared in TDDA process under certain conditions, further contributing 2.59% nitrogen removal and 10.46% sulfur removal (14.42 mg/L NH4+-N and 37.68 mg/L SO42--S were removed). This finding was mainly attributed to the reduction of sulfate in TDDA system to elemental S0 or HS-, which subsequently was used as an electron donor to realize the recycling of sulfate (SO42--S) pollutants and promote the sulfur-nitrogen (S-N) cycle. High-throughput analysis displayed that Anammox bacteria (Candidatus_Kuenenia), Sulfur-oxidizing bacteria (Thiobacillus) with relatively high abundance of 5.37%, 7.74%, respectively, guaranteeing the excellent nitrogen and sulfate removal performance in the reactor. The enrichment of phyla Chloroflexi (31.79%), Proteobacteria (31.82%), class Ignavibacteriales (10.55%), genus Planctomycetes (13.57%) further verified the exitence of Sulfammox process in the TDDA reactor. This study provides a new perspective for the practical application of TDDA in terms of reducing the production of high concentration SO42- and saving operational cost and strengthening deeply nitrogen removal.

Novel biotechnologies for nitrogen removal and their coupling with gas emissions abatement in wastewater treatment facilities.

Wastewaters contaminated with nitrogenous pollutants, derived from anthropogenic activities, have exacerbated our ecosystems sparking environmental problems, such as eutrophication and acidification of water reservoirs, emission of greenhouse gases, death of aquatic organisms, among others. Wastewater treatment facilities (WWTF) combining nitrification and denitrification, and lately partial nitrification coupled to anaerobic ammonium oxidation (anammox), have traditionally been applied for the removal of nitrogen from wastewaters. The present work provides a comprehensive review of the recent biotechnologies developed in which nitrogen-removing processes are relevant for the treatment of both wastewaters and gas emissions. These novel processes include the anammox process with alternative electron acceptors, such as sulfate (sulfammox), ferric iron (feammox), and anodes in microbial electrolysis cells (anodic anammox). New technologies that couple nitrate/nitrite reduction with the oxidation of methane, H2S, volatile methyl siloxanes, and other volatile organic compounds are also described. The potential of these processes for (i) minimizing greenhouse gas emissions from WWTF, (ii) biogas purification, and (iii) air pollution control is critically discussed considering the factors that might trigger N2O release during nitrate/nitrite reduction. Moreover, this review provides a discussion on the main challenges to tackle towards the consolidation of these novel biotechnologies.

Sulfate dependent ammonium oxidation: A microbial process linked nitrogen with sulfur cycle and potential application.

Sulfate dependent ammonium oxidation (Sulfammox) is a potential microbial process coupling ammonium oxidation with sulfate reduction under anaerobic conditions, which provides a novel link between nitrogen and sulfur cycle. Recently, Sulfammox was detected in wastewater treatments and was confirmed to occur in natural environments, especially in marine sediments. However, knowledge gaps in the mechanism of Sulfammox, functional bacteria, and their metabolic pathway, make it challenging to estimate its environmental significance and potential applications. This review provides an overview of recent advances in Sulfammox, including possible mechanisms, functional bacteria, and main influential factors, and discusses future challenges and opportunities. Future perspectives are outlined and discussed, such as exploration of microbial community structure and metabolic pathways, possible interactions with other microbes, environmental significance, and potential applications for nitrogen and sulfate removal, to inspire more researches on the Sulfammox process.