Novel, cold-adapted D-laminaribiose- and D-glucose-releasing GH16 endo-ß-1,3-glucanase from Hymenobacter siberiensis PAMC 29290, a psychrotolerant bacterium from Arctic marine sediment.

Endo-ß-1,3-glucanase is a glycoside hydrolase (GH) that plays an essential role in the mineralization of ß-glucan polysaccharides. In this study, the novel gene encoding an extracellular, non-modular GH16 endo-ß-1,3-glucanase (GluH) from Hymenobacter siberiensis PAMC 29290 isolated from Arctic marine sediment was discovered through an in silico analysis of its whole genome sequence and subsequently overexpressed in Escherichia coli BL21. The 870-bp GluH gene encoded a protein featuring a single catalytic GH16 domain that shared over 61% sequence identity with uncharacterized endo-ß-1,3-glucanases from diverse Hymenobacter species, as recorded in the National Center for Biotechnology Information database. The purified recombinant endo-ß-1,3-glucanase (rGluH: 31.0 kDa) demonstrated peak activity on laminarin at pH 5.5 and 40°C, maintaining over 40% of its maximum endo-ß-1,3-glucanase activity even at 25°C. rGluH preferentially hydrolyzed D-laminarioligosaccharides and ß-1,3-linked polysaccharides, but did not degrade D-laminaribiose or structurally unrelated substrates, confirming its specificity as a true endo-ß-1,3-glucanase without ancillary GH activities. The biodegradability of various substrate polymers by the enzyme was evaluated in the following sequence: laminarin > barley ß-glucan > carboxymethyl-curdlan > curdlan > pachyman. Notably, the specific activity (253.1 U mg-1) and catalytic efficiency (k cat /K m : 105.72 mg-1 s-1 mL) of rGluH for laminarin closely matched its specific activity (250.2 U mg-1) and k cat /K m value (104.88 mg-1 s-1 mL) toward barley ß-glucan. However, the k cat /K m value (9.86 mg-1 s-1 mL) of rGluH for insoluble curdlan was only about 9.3% of the value for laminarin, which correlates well with the observation that rGluH displayed weak binding affinity (< 40%) to the insoluble polymer. The biocatalytic hydrolysis of D-laminarioligosaccharides with a degree of polymerization between 3 and 6 and laminarin generally resulted in the formation of D-laminaribiose as the predominant product and D-glucose as the secondary product, with a ratio of approximately 4:1. These findings suggest that highly active rGluH is an acidic, cold-adapted D-laminaribiose- and D-glucose-releasing GH16 endo-ß-1,3-glucanase, which can be exploited as a valuable biocatalyst for facilitating low temperature preservation of foods.

Use of a Glaciogene Marine Clay (Ilulissat, Greenland) in a Pilot Production of Red Bricks.

Uplifted occurrences of fine-grained glaciogene marine sediments are found throughout the northern hemisphere. These sediments could be used to produce local construction materials, to rely less on imported construction materials from southern regions. In this study, a representative occurrence from Ilulissat, West Greenland, was investigated as a potential resource for local brick production. The study comprised three parts: (1) raw material characterization based on grain size distribution, major element chemistry, including total carbon, sulfur, and chloride concentrations, mineralogy, morphology, and Atterberg limits; (2) the production of test bricks at a Danish brickwork; and (3) testing of the bricks based on total shrinkage, water absorption, hygroscopic adsorption, open porosity, bulk density, compression strength, and mineralogy. The bricks produced proved to have excellent compression strength, low open porosity, and low water absorption. The shrinkage could be reduced by adding 10% chamotte to the marine sediment. Based on the investigated properties, this indicates that this type of clay is highly suitable as a resource for bricks.

Distribution of total mercury in coastal sediments of Honda Bay, Palawan Island, the Philippines.

Honda Bay is considered as one of the mercury hotspots in the world due to its proximity to the abandoned Palawan Quicksilver Mine. In this study, a detailed sediment sampling conducted in between 2021 and 2022 where a total of 166 sediment samples were collected along the coast and analyzed for total mercury (THg) concentration. The study assessed mercury toxicity using the geoaccumulation index and compared Hg levels to sediment quality guidelines. The findings revealed a wide range of THg concentrations, from 0.0040 to 11.4702 mg/kg, with hotspots identified at the Honda Bay wharf and Tagburos River mouth. Mercury spreads to a large coastal area brought by tidal currents and the wave energy actions. The geoaccumulation index indicated moderate to strong Hg contamination in the vicinity of the hotspots and around 24.7-36.1 % of samples exceeded the sediment quality guidelines suggesting adverse biological effects in aquatic biota will frequently occur.

Critical reassessment of microplastic abundances in the marine environment.

Microplastics (MPs) pose a growing concern in the marine environment, but their global prevalence remains largely unknown due to the absence of precise and standardized detection methods. This review critically evaluates existing techniques for quantifying MP abundances in marine field studies, addressing inaccuracies resulting from the exclusion of particle sizes, polymer types, or limitations in identification methods. These traced inaccuracies were considered to recalculate MP abundances for particle sizes from 10 to 5000 µm, providing the first corrected global overview of MP distribution that enables quality assessment and reliable comparisons between adjusted data. The recalculations indicate that MP abundances are up to 15 times higher in marine waters (average (1.5 ± 36.2) × 105 items m-3) and up to 11 times higher in the marine sediments (average (2.7 ± 117.9) × 105 items kg-1) than previously reported in the literature. The Australasian Mediterranean Sea (average (1.2 ± 10.6) × 106 items m-3) and the North Atlantic (average (2.1 ± 37.6) × 105 items kg-1) emerged as the most polluted regions in marine waters and sediments, respectively, with primary contributors being the coasts of Southeast Asia and East America. This review demonstrates that previous field studies, global estimates, and models have significantly underestimated MP levels in marine environments in many cases, which could result in misinterpretations of both local and global pollution levels. This work highlights the critical need for precise handling of microplastic samples and urges future researchers to adopt standardized protocols for MP analysis to avoid inaccurate and misleading outcomes.

Comparative analysis of microplastics detection methods applied to marine sediments: A case study in the Bay of Marseille.

An intercomparison exercise on "microplastics in sediment" was carried out by five laboratories using samples collected in the Bay of Marseille in September 2021. The results from different extraction and identification methods varied depending on the type and size classes of MPs, and was better than 80 % for the size class >300 µm and for the fragments. The variability in recovery rates can be attributed to the choice of reagents and extraction protocols. Recovery rates per laboratory were between 47 % and 113 % and the use of ZnCl2 and NaI increased recovery rates by an average of 70 %. The lowest recovery rates (47 and 53 %) were attributed to the reference methods (FTIR and LDIR), conversely the highest (80 and 87 %) were attributed to identification by Nile Red. The average ranged between 23 and 53 items /50 g d.w. with decreases offshore and at greater depth.

Determining the abundance, composition and spatial distribution of organohalogens in marine sediments using combustion-ion chromatography.

Understanding the distribution of halogenated organic compounds (HOCs) in marine sediments is essential for understanding the marine carbon and halogen cycling, and also important for assessing the ecosystem health. In this study, a method based on combustion-ion chromatography was developed for determination of the composition and abundance of HOCs in marine sediments. The method showed high accuracy, precision and reproducibility in determining the content of adsorbable organic halogens (AOX), including fluorine, chlorine and bromine (AOF, AOCl, AOBr) and the corresponding insoluble organic halogens (IOF, IOCl, IOBr, IOX), as well as total organic halogen contents (TOX). Application of the method in coastal and deep-sea sediments revealed high ratios of organic halogens in the organic carbon pool of marine sediments, suggesting that organic halogen compounds represent an important yet previously overlooked stock of carbon and energy in marine sediments. Both the TOX and the proportion of organohalogens in organic carbon (X:C ratio) showed an increasing trend from the coast to the deep-sea sediments, indicating an increased significance of HOCs in deep-sea environments. The developed method and the findings of this study lay the foundation for further studies on biogeochemical cycling of HOCs in the ocean.

Six novel species of the genus Methanoculleus isolated from various environments in Taiwan.

Taiwan is situated in the subtropical region and its geographical location and topographical features contribute to a rich ecological diversity and scenic landscapes. We investigated the diversity of methanogens in different environments of Taiwan using a culture-dependent method. This report presents the characterization and taxonomy of six hydrogenotrophic methanogens obtained from cold seep sediments (strain FWC-SCC1T and FWC-SCC3T), marine sediments (strain CWC-02T and YWC-01T), estuarine sediments (strain Afa-1T), and a hot spring well (strain Wushi-C6T) in Taiwan. The proposed names of the six novel species are Methanoculleus frigidifontis (type strain FWC-SCC1T=BCRC AR10056T=NBRC 113993T), Methanoculleus oceani (CWC-02T=BCRC AR10055T=NBRC 113992T), Methanoculleus methanifontis (FWC-SCC3T=BCRC AR10057T=NBRC 113994T), Methanoculleus nereidis (YWC-01T=BCRC AR10060T=NBRC 114597T), Methanoculleus formosensis (Afa-1T=BCRC AR10054T=NBRC 113995T), and Methanoculleus caldifontis (Wushi-06T=BCRC AR10059T= NBRC 114596T).

A new certified reference material IAEA-465 for radionuclides in Baltic Sea sediment.

Certified reference material (CRM) for natural (40K,210Pb,210Po,226Ra,228Ra,228Th,230Th,232Th,234U,235U, and238U) and anthropogenic (137Cs,239+240Pu, and241Am) radionuclides in marine sediment from the Baltic Sea (IAEA-465) has been developed. Information values are given for 238Pu,239Pu and240Pu. Altogether 27 laboratories participated in this exercise. Radiometric (alpha-spectrometry, gamma-spectrometry and beta counting, as well as mass spectrometry (ICP-MS and AMS) techniques were applied in measurements. The CRM is intended to be used for Quality Assurance/Quality Control of radionuclide analyses, for the development and validation of analytical methods, for the development of reference methods and for training purposes.

Nationwide monitoring of legacy and emerging persistent organic pollutants and polycyclic aromatic hydrocarbons along the Korean coast.

Comprehensive studies simultaneously investigating the occurrence of chemicals of concern are limited. In this study, sediments and bivalves were collected from 24 locations along the Korean coast to evaluate the relative distribution, contamination characteristics, and ecological risks of legacy/emerging persistent organic pollutants (POPs) and polycyclic aromatic hydrocarbons (PAHs). Our findings reveal that the concentrations of these contaminants were comparable to or lower than historical levels in the same Korean coast and other Asian countries. Notably, PAHs exhibited the highest distribution in sediments (84 %), whereas short-chain chlorinated paraffins (SCCPs) were dominant in bivalves (91 %). This study highlighted significant correlations in the sediment levels of each legacy pollutants, suggesting similar sources and geochemical behaviors. However, SCCPs displayed unique contamination patterns. Ecologically, PAHs and SCCPs presented low risks in sediments compared to Canadian Sediment Quality Guidelines, however 100 % and 33 % of bivalves, respectively, exceeded US EPA/Canadian Fish Tissue Guidelines.

Assessment of microplastic presence in coastal environments and organisms of Da Nang, Vietnam.

This study investigates the presence of microplastics (MPs) in seawater, sediments, and organisms along the coastal areas of Da Nang, Vietnam. The results obtained revealed MP concentrations ranging from 111 to 304 MPs/L in seawater and 2267 to 4600 MPs/kg in sediment. In organisms such as oysters, mussels, crabs, snails, and fish, MP levels ranged from 1.8 to 17.3 MPs/g (wet weight). Fiber MPs were found to be predominant across seawater, sediment, and organisms. The study identified eight, ten, and eleven types of MPs in seawater, sediment, and organisms, respectively, with Nylon, Polytetrafluoroethylene (PTFE), and Ethylene vinyl alcohol (EVOH) being the most prevalent. Notably, MP concentrations were significantly higher in benthic organisms such as oysters, mussels, and crabs compared to fish (t-test, p < 0.05), suggesting habitat dependency. Similar concentrations, shapes, and types of MPs in seawater, sediments, and organisms demonstrate a tendency for MP accumulation in aquatic organisms within the marine environment.

Microbial communities as indicators of marine ecosystem health: Insights from coastal sediments in the eastern Adriatic Sea.

Considering the adaptability and responsiveness of microorganisms to environmental changes, their indicator potential is still not acknowledged in European directives. This comprehensive study examined the changes of microbial communities in sediments and a range of geochemical parameters from pristine and anthropogenically impacted coastal areas in the eastern Adriatic Sea. Various analytical methods found evidence of sediment contamination (high toxicity level, enrichments of metals, tributyltin) in certain areas, leading to the categorization of sediments based on the level of anthropogenic disturbance. Prokaryotes were identified as the most promising group of microbes for further research, with specific bacterial families (Rhodobacteraceae, Ectothiorhodospiraceae, Cyclobacteriaceae) and genera (Boseongicola, B2M28, Subgroup 23, Sva0485, Thiogranum) proposed as potential indicators of environmental status. Finally, predictive models were developed to identify key indicator variables for assessing anthropogenic impact in sediments. This research represents an essential step toward incorporating microbial communities into assessments of benthic environmental health.

The Antibacterial Efficacy of a Compound Extracted from Marine Sediment Bacterium Enterococcus Lactis (S-2): A Comparative Analysis Through In-Vitro and In-Silico Assessments.

BACKGROUND: Marine sediment bacteria have been generating considerable attention lately due to their potential as valuable reservoirs of novel antimicrobial agents. AIM: In vitro and in silico antibacterial activities of antibacterial compounds isolated from the marine sediment bacterium Enterococcus Lactis (S-2). METHODS: Coastal sediment samples were collected from Rameswaram, Ramnathapuram District, Tamil Nadu, India. Bacteria were isolated using the crowded plate method, and their phenotypic and genotypic characteristics were studied. Purified bacteria were cultured in large volumes, secondary metabolites were extracted, and novel antibacterial agents were isolated from the aqueous extract. Novel compound antibacterial activity was studied through in-silico and invitro. The mechanism activity of antibacterial activity was confirmed by a high-resolution transmission electron microscope. RESULTS: Genotypic analysis confirmed that the isolated S-2 bacteria were Enterococcus lactis, and the aqueous extract showed antibacterial activity against Staphylococcus aureus (17 mm zone of inhibition) and Proteus mirabilis (12 mm zone of inhibition). A bioactive molecule, 13- hydroxy-9-(1-hydroxyethyl)-11-methoxy-2,4dioxapentacyclo[10.7.1.0³,4.05,²¹.0¹³,¹6]icosa- 1(20),5,7,12,14(19), 16-hexane-18-one, was isolated from aqueous extracts of the S-2 bacterium. Chromatography and spectroscopic analysis confirmed the identity of the isolated compound. Novel compound potential antibacterial activity showing against S. aureus (18 mm zone of inhibition) and MIC 250 µg/mL, which was confirmed by tetrazolium staining. The antibacterial activity mechanism was confirmed by transmission electron microscopy. Molecular docking studies show good binding (-9.9 kcal/mol) of the compound with 3U2D, while molecular dynamic simulation studies confirm the conformationally stable structure of the complex between 3U2D and 13-hydroxy-9-(1-hydroxyethyl)-11-methoxy-2,4-dioxapentacyclo [10.7.1.0³,4.05,²¹.0¹³,¹6]icosa-1(20),5,7,12,14(19), 16-hexane-18-one. It has been observed from the docking study of 3U2D with standard drug ciprofloxacin that the lower affinity is compared to the test ligand, which has a docking score of 7.3 kcal/mol. Out of interacting residues of protein 3U2D residue, Thr173 and Ile86 formed conventional hydrogen bonds. CONCLUSION: Marine bacterium E. lactis produces a novel antibacterial compound (13-hydroxy- 9-(1-hydroxyethyl)-11-methoxy-2,4-dioxapentacyclo[10.7.1.0³,4.05,²¹.0¹³,¹6]icosa- 1(20),5,7,12,14(19),16-hexane-18-one), which shows antibacterial activity against clinical S. aureus, confirmed by in vitro and in silico analysis. This molecule can used as a lead molecule for antibacterial activity.

Enhanced sinks of polystyrene nanoplastics (PSNPs) in marine sediment compared to freshwater sediment: Influencing factors and mechanisms.

The difference in the transport behaviors of nanoplastics consistently assistant with their toxicities to benthic and other aquatic organisms is still unclear between freshwater and marine sediments. Here, the mobilities of polystyrene nanoplastics (PSNPs) and key environmental factors including salinity and humic acid (HA) were systematically studied. In the sand column experiments, both tested PSNPs in the freshwater system (100 nm NPs (100NPs): 90.15 %; 500 nm NPs (500NPs): 54.22 %) presented much higher penetration ratio than in the marine system (100NPs: 8.09 %; 500NPs: 19.04 %). The addition of marine sediment with a smaller median grain diameter caused a much more apparent decline in NPs mobility (100NPs: from 8.09 % to 1.85 %; 500NPs: from 19.04 % to 3.51 %) than that containing freshwater sediment (100NPs: from 90.15 % to 83.56 %; 500NPs: from 54.22 % to 41.63 %). Interestingly, adding HA obviously led to decreased and slightly increased mobilities for NPs in freshwater systems, but dramatically improved performance for NPs in marine systems. Electrostatic and steric repulsions, corresponding to alteration of zeta potential and hydrodynamic diameter of NPs and sands, as well as minerals owing to adsorption of dissolved organic matter (DOM) and aggregations from varied salinity, are responsible for the mobility difference.

Phytohabitans maris sp. nov., isolated from marine sediment.

A novel actinobacterium, strain ZYX-F-186T, was isolated from marine sediment sampled on Yongxing Island, Hainan Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain ZYX-F-186T belongs to the genus Phytohabitans, with high similarity to Phytohabitans kaempferiae KK1-3T (98.3 %), Phytohabitans rumicis K11-0047T (98.1 %), Phytohabitans flavus K09-0627T (98.1 %), Phytohabitans houttuyneae K11-0057T (97.9 %), Phytohabitans suffuscus K07-0523T (97.7 %), and Phytohabitans aurantiacus RD004123T (97.7 %). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a single subclade in the genus Phytohabitans. The novel isolate contained meso-diaminopimelic acid, d-glutamic acid, glycine, d-alanine, and l-lysine in the cell wall. The whole-cell sugars were xylose, arabinose, ribose, and rhamnose. The predominant menaquinones were MK-9(H8), MK-9(H6), and MK-9(H4). The characteristic phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The major fatty acids (>5 %) were iso-C16 : 0, anteiso-C17 : 0, and iso-C18 : 0. Genome sequencing showed a DNA G+C content of 71.9 mol%. Low average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values demonstrated that strain ZYX-F-186T could be readily distinguished from its closely related species. Based on its phylogenetic, chemotaxonomic, and physiological characteristics, strain ZYX-F-186T represents a novel species of the genus Phytohabitans, for which the name Phytohabitans maris sp. nov. is proposed. The type strain is ZYX-F-186T (=CGMCC 4.8025T=CCTCC AA 2023025T=JCM 36507T).

A review of spatial distribution of typical antibiotic resistance genes in marine environment surrounding China.

Antibiotic resistance genes (ARGs) have been steadily increasing due to the extensive overuse of antibiotics in the marine environment. Currently, the research considering ARGs distribution in marine ecosystems gains more interest. As the coastal sea has been regarded as one of the most polluted areas by antibiotic contaminants in China. However, no comprehensive review of the spatial distribution of ARGs in marine environment surrounding China. The main objective of this review is to investigate the level, characteristic, and spatial distribution of ARGs in the marine environment (seawater and sediments) surrounding China. Key sea areas, such as Bohai Sea, Yellow Sea, East China Sea, and South China Sea were selected in this review. The marine environment was the reservoir of ARGs, and ARGs in seawater were generally 1 to 2 orders of magnitude higher than that in sediments. Total ARGs were more abundant in the Yellow Sea, followed by the Bohai Sea, the East China Sea, and the South China Sea. This study raises questions regarding the spread and distribution for antibiotic resistance in marine environments.

Marine sediments in the Gulf of Gabes (Tunisia) heavily polluted by phosphogypsum and human microbiota bacteria: phytoremediation by Salicornia europaea as a natural-based solution.

A huge amount of phosphogypsum (PG) wastes generated from the processing phosphate ore in Tunisia Industrial Group Area-Gabes is getting discarded into the sea. Within this framework, the basic objective of this research is to elaborate and discuss a natural-based solution focused on phytoremediation of contaminated (PG) soils and marine sediments with the halophilic plant Salicornia europaea. A significant drop of the organic matter (53.09%), moisture (26.47%), and sediment porosity with (5.88%) was detected in the rhizosphere Salicornia europaea area (RS). Removal of hazardous elements concentrations, such as Pb, Fe, Cu, Cd, and Zn, between contaminated sediment (CS) and RS displayed a significant difference, ranging from 5.33 to 50.02% of hazardous elements removal concentration, which was observed in the rhizosphere zone. The microbiota of both areas (RS and CS) were analyzed by massive sequencing. In both samples, all the sequences belong to only four phyla: Firmicutes and, to a much lower extent, Proteobacteria, Bacteroidetes, and Actinobacteria. The CS sediment seems to be heavily polluted by human activities. Most of the found genera are inhabitants of the intestine of warm-blooded animals (Escherichia, Bacteroides, Prevotella, Faecalibacterium, Ruminococcus, Enterococcus); hence, activities in this area pose a health risk. On the other hand, it may be surprising that 76.4% of the total high-quality sequences retrieved from the RS sample were affiliated to the family Bacillaceae. The salinity of the studied soil exerts a stress on the microbial populations that inhabit it, directing the selection of halotolerant species.

Actomonas aquatica gen. nov., sp. nov., a marine lineage in the phylum Verrucomicrobiota with genetic potential for carbon and nitrogen fixation.

A novel bacterial strain, designated WL0086T, was isolated from a marine sediment sample collected in Lianyungang city, Jiangsu province, PR China. This strain showed the highest 16S rRNA gene sequence similarity to Geminisphaera colitermitum TAV2T (92.7 %) of the family Opitutaceae, and all the unclassified cultured and uncultured isolates with similarities >95 % were from marine environments. Cells were Gram-stain-negative, aerobic, non-motile cocci with a size of 0.6-0.8 µm in diameter. Strain WL0086T was positive for both oxidase and catalase, and grew at 20-37 °C (optimum, 28 °C), with 1.5-11.0 % NaCl (w/v; optimum, 2.5-4.0 %) and at pH 5.0-9.0 (optimum, pH 7.0). The major polar lipid profile of strain WL0086T consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine. The major isoprenoid quinone was menaquinone-7 and the predominant fatty acids were iso-C14 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1 ω9c. The complete genome consisted of a chromosome with 6 109 182 bp. The G+C content of genomic DNA was 64.0%. Results of phylogenomic analysis based on the 16S rRNA gene sequence and the whole genome suggested that strain WL0086T formed a distinct clade closely neighbouring the members of the family Opitutaceae. On the basis of phylogenetic, phenotypic, and chemotaxonomic evidences, strain WL0086T should represent a novel genus of the family Opitutaceae, for which the name Actomonas aquatica gen. nov., sp. nov. is proposed. The type strain is WL0086T (=MCCC 1K05844T=JCM 34677T=GDMCC 1.2411T).

Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan.

A novel mesophilic, hydrogenotrophic methanogen, strain CWC-04T, was obtained from a sediment sample extracted from a gravity core retrieved at station 22 within the KP-9 area off the southwestern coast of Taiwan during the ORIII-1368 cruise in 2009. Cells of strain CWC-04T were rod-shaped, 1.4-2.9 µm long by 0.5-0.6 µm wide, and occurred singly. Strain CWC-04Tutilized formate, H2/CO2, 2-propanol/CO2 or 2-butanol/CO2 as catabolic substrates. The optimal growth conditions were 42 °C, 0.17 M NaCl and pH 5.35. The genomic DNA G+C content calculated from the genome sequence of strain CWC-04T was 46.19 mol%. Phylogenetic analysis of 16S rRNA gene revealed that strain CWC-04T is affiliated with the genus Methanocella. The 16S rRNA gene sequences similarities within strains Methanocella arvoryzae MRE50T, Methanocella paludicola SANAET and Methanocella conradii HZ254T were 93.7, 93.0 and 91.3 %, respectively. In addition, the optical density of CWC-04T culture dropped abruptly upon entering the late-log growth phase, with virus-like particles (150 nm in diameter) being observed on and around the cells. This observation suggests that strain CWC-04T harbours a lytic virus. Based on these phenotypic, phylogenetic and genomic results, we propose that strain CWC-04T represents a novel species of a novel genus in the family Methanocellaceae, for which the name Methanooceanicella nereidis gen. nov., sp. nov. is proposed. The type strain is CWC-04T (=BCRC AR10050T=NBRC 113165T).

Microbaculum marinisediminis sp. nov., isolated from marine sediment.

A novel Gram-stain-negative and facultatively anaerobic bacterium, designated A6E488T, was isolated from intertidal sediment collected from Xiaoshi Island, Weihai, PR China (122° 1' E 37° 31' N). Cells of strain A6E488T were rod-shaped with widths of 0.3-0.4 µm and lengths of 1.1-1.8 µm. The optimal growth conditions were determined to be in 1 % (w/v) NaCl, at 37 °C, and at pH 7.0. The predominant fatty acids (≥10 %) were C19 : 0 cyclo ω8c (59.7 %) and summed feature 8 (13.8 %, C18 : 1 ω7c and/or C18 : 1 ω6c). The sole isoprenoid quinone was Q-10. Oxidase activity was negative but catalase activity was positive. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, one unidentified glycolipid, and one unidentified lipid. Based on phylogenetic analysis of 16S rRNA gene sequences, strain A6E488T showed the highest sequence similarity to Microbaculum marinum MCCC 1K03192T (97.6 %). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain A6E488T and M. marinum MCCC 1K03192T did not exceed 78 and 22 %, respectively. These values are below the recommended thresholds of 95 % (ANI) and 70 % (dDDH) for prokaryotic species delineation. On the basis of gene annotation, it was observed that strain A6E488T possesses the capability for thiosulphate oxidation, suggesting that this strain might be important in the sulphur cycle. Based on the results of phenotypic, genotypic, and chemical characterization, strain A6E488T is considered to represent a novel species of the genus Microbaculum, for which the name Microbaculum marinisediminis sp. nov. is proposed. The type strain is A6E488T (=KCTC 92197T=MCCC 1H00516T).

Occurrence and prevalence of per- and polyfluoroalkyl substances in the sediment pore water of mariculture sites: Novel findings of PFASs from the Bohai and Yellow Seas using a newly established analytical method.

A new method for the determination of 26 legacy and emerging per- and polyfluoroalkyl substances (PFASs) in marine sediment pore water was developed using online solid phase extraction coupled with liquid chromatography-tandem mass spectrometry. The proposed method requires only about 1 mL of pore water samples. Satisfactory recoveries of most target PFASs (83.55-125.30 %) were achieved, with good precision (RSD of 1.09-16.53 %), linearity (R2 ≥ 0.990), and sensitivity (MDLs: 0.05 ng/L-5.00 ng/L for most PFASs). Subsequently, the method was applied to determine PFASs in the sediment pore water of five mariculture bays in the Bohai and Yellow Seas of China for the first time. Fifteen PFASs were detected with total concentrations ranging from 150.23 ng/L to 1838.48 ng/L (mean = 636.80 ng/L). The ∑PFASs and PFOA concentrations in sediment pore water were remarkably higher than those in surface seawater (tens of ng/L), indicating that the potential toxic effect of PFASs on benthic organisms may be underestimated. PFPeA was mainly distributed in pore water, and the partition of PFHpA (50.99 %) and PFOA (49.01 %) was almost equal in the solid and liquid phases. The proportions of all other PFASs partitioned in marine sediments were significantly higher than those in pore water.