RESUMEN
Dietary supplementation of the amino acid histidine has demonstrable benefits in various clinical conditions. Recent work in a pediatric leukemia mouse model exposed a surprising potential application of histidine supplementation for cancer therapy enhancement. These findings demand a deeper reassessment of the physiological effects and potential drawbacks of histidine supplementation. As pertinent to this question, we discuss the safety of high doses of histidine and its relevant metabolic fates in the human body. We refrain from recommendations or final conclusions because comprehensive preclinical evidence for safety and efficacy of histidine supplementation is still lacking. However, we emphasize the incentive to study the safety of histidine supplementation and its potential to improve the clinical outcome of pediatric blood cancers through a simple dietary supplementation. The need for comprehensive preclinical testing of histidine supplementation in healthy and tumor-bearing mice is fundamental, and we hope that this review will facilitate such studies.
Asunto(s)
Suplementos Dietéticos , Histidina/metabolismo , Histidina/farmacología , Neoplasias/metabolismo , Animales , Ácido Fólico/metabolismo , Ácido Formiminoglutámico/metabolismo , Histidina/efectos adversos , Histidina/uso terapéutico , Humanos , Leucemia/tratamiento farmacológico , Metotrexato/uso terapéutico , Neoplasias/tratamiento farmacológicoRESUMEN
ALDH1L1 (10-formyltetrahydrofolate dehydrogenase), an enzyme of folate metabolism highly expressed in liver, metabolizes 10-formyltetrahydrofolate to produce tetrahydrofolate (THF). This reaction might have a regulatory function towards reduced folate pools, de novo purine biosynthesis, and the flux of folate-bound methyl groups. To understand the role of the enzyme in cellular metabolism, Aldh1l1-/- mice were generated using an ES cell clone (C57BL/6N background) from KOMP repository. Though Aldh1l1-/- mice were viable and did not have an apparent phenotype, metabolomic analysis indicated that they had metabolic signs of folate deficiency. Specifically, the intermediate of the histidine degradation pathway and a marker of folate deficiency, formiminoglutamate, was increased more than 15-fold in livers of Aldh1l1-/- mice. At the same time, blood folate levels were not changed and the total folate pool in the liver was decreased by only 20%. A two-fold decrease in glycine and a strong drop in glycine conjugates, a likely result of glycine shortage, were also observed in Aldh1l1-/- mice. Our study indicates that in the absence of ALDH1L1 enzyme, 10-formyl-THF cannot be efficiently metabolized in the liver. This leads to the decrease in THF causing reduced generation of glycine from serine and impaired histidine degradation, two pathways strictly dependent on THF.
Asunto(s)
Glicina/metabolismo , Hígado/enzimología , Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH/metabolismo , Animales , Femenino , Ácido Formiminoglutámico/análisis , Ácido Formiminoglutámico/metabolismo , Glicina/análisis , Histidina/metabolismo , Leucovorina/análogos & derivados , Leucovorina/metabolismo , Hígado/química , Masculino , Ratones , Ratones Noqueados , Modelos Animales , Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH/genética , Serina/metabolismo , Tetrahidrofolatos/biosíntesisRESUMEN
The crystal structure of formiminoglutamase from Trypanosoma cruzi (TcFIGase) is reported at 1.85 Å resolution. Although the structure of this enzyme was previously determined by the Structural Genomics of Pathogenic Protozoa Consortium (PDB accession code 2A0M), this structure was determined at low pH and lacked bound metal ions; accordingly, the protein was simply annotated as "arginase superfamily protein" with undetermined function. We show that reconstitution of this protein with Mn²âº confers maximal catalytic activity in the hydrolysis of formiminoglutamate to yield glutamate and formamide, thereby demonstrating that this protein is a metal-dependent formiminoglutamase. Equilibration of TcFIGase crystals with MnCl2 at higher pH yields a binuclear manganese cluster similar to that observed in arginase, except that the histidine ligand to the Mn²âº(A) ion of arginase is an asparagine ligand (N114) to the Mn²âº(A) ion of TcFIGase. The crystal structure of N114H TcFIGase reveals a binuclear manganese cluster essentially identical to that of arginase, but the mutant exhibits a modest 35% loss of catalytic efficiency (k(cat)/K(M)). Interestingly, when TcFIGase is prepared and crystallized in the absence of reducing agents at low pH, a disulfide linkage forms between C35 and C242 in the active site. When reconstituted with Mn²âº at higher pH, this oxidized enzyme exhibits a modest 33% loss of catalytic efficiency. Structure determinations of the metal-free and metal-bound forms of oxidized TcFIGase reveal that although disulfide formation constricts the main entrance to the active site, other structural changes open alternative channels to the active site that may help sustain catalytic activity.
Asunto(s)
Ácido Formiminoglutámico/metabolismo , Hidrolasas/química , Metaloproteínas/química , Modelos Moleculares , Proteínas Protozoarias/química , Trypanosoma cruzi/enzimología , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Apoenzimas/química , Apoenzimas/genética , Apoenzimas/metabolismo , Arginasa/química , Arginasa/metabolismo , Asparagina/química , Sitios de Unión , Secuencia Conservada , Cisteína/química , Hidrolasas/genética , Hidrolasas/metabolismo , Manganeso/análisis , Manganeso/química , Manganeso/metabolismo , Metaloproteínas/genética , Metaloproteínas/metabolismo , Datos de Secuencia Molecular , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , Conformación Proteica , Proteínas Protozoarias/genética , Proteínas Protozoarias/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alineación de SecuenciaRESUMEN
Pseudomonas proliferate in a wide spectrum of harsh and variable environments. In many of these environments, amino acids, such as histidine, are a valuable source of carbon, nitrogen and energy. Here, we demonstrate that the histidine uptake and utilization (hut) pathway of Pseudomonas aeruginosa PAO1 contains two branches from the intermediate formiminoglutamate to the product glutamate. Genetic analysis revealed that the four-step route is dispensable as long as the five-step route is present (and vice versa). Mutants with deletions of either the four-step (HutE) or five-step (HutFG) branches were competed against each other and the wild-type strain to test the hypothesis of ecological redundancy; that is, that the presence of two pathways confers no benefit beyond that delivered by the individual pathways. Fitness assays performed under several environmental conditions led us to reject this hypothesis; the four-step pathway can provide an advantage when histidine is the sole carbon source. An IclR-type regulator (HutR) was identified that regulates the four-step pathway. Comparison of sequenced genomes revealed that P.aeruginosa strains and P.fluorescens Pf-5 have branched hut pathways. Phylogenetic analyses suggests that the gene encoding formiminoglutamase (hutE) was acquired by horizontal gene transfer from a Ralstonia-like ancestor. Potential barriers to inter-species transfer of the hutRE module were explored by transferring it from P.aeruginosa PAO1 to P.fluorescens SBW25. Transfer of the operon conferred the ability to utilize histidine via the four-step pathway in a single step, but the fitness cost of acquiring this new operon was found to be environment dependent.
Asunto(s)
Ambiente , Histidina/metabolismo , Pseudomonas aeruginosa/metabolismo , Ácido Formiminoglutámico/metabolismo , Regulación Bacteriana de la Expresión Génica , Genoma Bacteriano/genética , Ácido Glutámico/biosíntesis , Mutación , Operón/genética , Filogenia , Pseudomonas aeruginosa/clasificación , Pseudomonas aeruginosa/genéticaRESUMEN
The vulnerability of the nervous system due to methanol (MeOH) intoxication is a well known fact and reports on the production of free radicals due to MeOH exposure and their involvement in excitotoxicity and neuronal apoptosis are being increasingly reported. We report on MeOH induced free radical changes and oxidative damages to proteins in the discrete regions of rat brain, retina and optic nerve. The present study used rats administered with methotrexate (MTX) to induce folate deficiency. Three groups of animals, namely saline control, MTX control, MTX-MeOH group were tested. The rats were injected intraperitoneally with MeOH (3 g/kg). After 24 h of MeOH administration, the levels of free radical scavengers, superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione levels were estimated in the six discrete regions of brain (cerebral cortex, cerebellum, midbrain, pons medulla, hippocampus and hypothalamus), retina and optic nerve specimens. The levels of protein thiol, protein carbonyl and lipid peroxidation were also estimated and the expression of HSP70 in the hippocampus was analyzed by Western blot. Marked reduction in the levels of glutathione in the MTX-MeOH group in relation to MTX control was observed and found to be increased in MTX control in relation to saline control. Increased protein carbonyls and decreased protein thiols were documented in all the specimens tested. In addition, marked expression of HSP70 was observed in the hippocampus. The present investigation suggest that MeOH exposure results in increased generation of free radicals and significant protein oxidative damage and attempts to study the underlying mechanisms involved might reveal more insights to our existing knowledge on MeOH intoxication and related areas.
Asunto(s)
Encéfalo/efectos de los fármacos , Metanol/toxicidad , Metotrexato/toxicidad , Nervio Óptico/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Retina/efectos de los fármacos , Animales , Catalasa/metabolismo , Quimioterapia Combinada , Formiatos/sangre , Ácido Formiminoglutámico/metabolismo , Regulación de la Expresión Génica , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Proteínas HSP70 de Choque Térmico/metabolismo , Peroxidación de Lípido , Masculino , Metanol/sangre , Ratas , Ratas Wistar , Compuestos de Sulfhidrilo/sangre , Superóxido Dismutasa/sangreRESUMEN
INTRODUCTION: Previous studies have given conflicting results as to whether or not folate metabolism is altered in Down syndrome. Folate is necessary to facilitate metabolism of one-carbon units. Folate accepts one-carbon units from one-carbon unit donors, including formiminoglutamate (FIGLU). Folate deficiency leads to accumulation of FIGLU and impairment of one-carbon unit metabolism. FIGLU is a functional measure of folate deficiency. MATERIALS AND METHODS: Archived anonymized amniotic fluid specimens were obtained from normal pregnancies and those with Down syndrome. Gas liquid chromatography/mass spectrometry was used to quantitate FIGLU, which is elevated in folate deficiency. A tetra-deuterated FIGLU was used as a standard, and single-ion monitoring was performed. Nonparametric statistical analysis was performed with the Mann-Whitney U test. RESULTS: FIGLU was significantly lower in pregnancies with Down syndrome. The median FIGLU level was 0.9 micromol/l in amniotic fluid from fetuses with Down syndrome. The median FIGLU level was 1.3 in amniotic fluid from control fetuses. This difference was statistically significant (p = 0.009). No statistically significant differences were found with histidine or glutamate. DISCUSSION: There was no evidence of folate deficiency. FIGLU was decreased, not increased. Decreased FIGLU might result from accelerated activity of one or more genes on chromosome 21, by a gene dosage effect. Genes which might explain the reduced FIGLU include one which degrades FIGLU (glutamate formiminotransferase-cyclodeaminase), one which participates in purine synthesis, and one which degrades homocysteine (cystathionine-beta-synthase).
Asunto(s)
Líquido Amniótico/metabolismo , Síndrome de Down/metabolismo , Ácido Formiminoglutámico/metabolismo , Amniocentesis , Síndrome de Down/diagnóstico , Femenino , Ácido Fólico/metabolismo , Humanos , EmbarazoRESUMEN
A previous experiment using rats indicated that dietary nickel (Ni), folic acid, and their interaction affected variables associated with one-carbon metabolism. That study used diets that produced only mild folate deficiency. Thus, an experiment was performed to determine the effect of a severe folate deficiency on nickel deprivation in rats. A 2 x 2 factorially arranged experiment used groups of six weanling Sprague-Dawley rats. Dietary variables were nickel, as NiCl2-6H2O, 0 or 1 microgram/g and folic acid, 0 or 4 mg/kg. All diets contained 10 g succinylsulfathiazole/kg to suppress microbial folate synthesis. The basal diet contained < 20 ng Ni/g. After 58 d, an interaction between nickel and folate affected the urinary excretion of formiminoglutamic acid (FIGLU) and the liver concentration of S-adenosylmethionine (SAM). Because of this, it is proposed that the physiological function of nickel is related to the common metabolism shared by SAM and FIGLU. Possibly the physiological function of nickel could be related to the tissue concentration of 5-methyltetrahydrofolate (MTHF) or tetrahydrofolate (THF).
Asunto(s)
Deficiencia de Ácido Fólico/metabolismo , Metionina/metabolismo , Níquel/metabolismo , Animales , Ácido Fólico/farmacología , Ácido Formiminoglutámico/metabolismo , Hígado/metabolismo , Masculino , Níquel/farmacología , Ratas , Ratas Sprague-Dawley , S-Adenosilhomocisteína/metabolismo , S-Adenosilmetionina/metabolismo , Sulfatiazoles/farmacologíaRESUMEN
Exposure to nitrous oxide (N2O) markedly enhances excretion of formic acid and formiminoglutamic acid (FIGLU) in the urine of rats, suggesting a disruption in the normal pathways of folic acid metabolism secondary to an N2O-induced inactivation of methionine synthase. We tested whether surgical patients (23 having total hip replacements and 26 having resection of acoustic neuromas) exposed to isoflurane alone or combined with N2O responded similarly. We found no increase in urinary formic acid and FIGLU in patients exposed to N2O for hip replacement, but a small, transient increase in the FIGLU-to-creatinine ratio in those undergoing resection of acoustic neuromas (mean duration of anesthesia = 9.3 h). This increase peaked at the end of anesthetic exposure and returned toward control levels by the first day after anesthesia and surgery. Low preoperative levels of red blood cell folate and low-normal levels of serum vitamin B12 did not predict an increase in formic acid or FIGLU in response to N2O. Although an occasional patient may prove highly susceptible to and develop signs of severe vitamin B12 and folic acid deficiency after exposure to N2O, our findings suggest that this is a rare event.
Asunto(s)
Ácido Fólico/metabolismo , Óxido Nitroso/farmacología , Vitamina B 12/metabolismo , Adulto , Anciano , Envejecimiento/metabolismo , Femenino , Ácido Formiminoglutámico/metabolismo , Humanos , Masculino , Persona de Mediana Edad , Periodo Posoperatorio , Medicación Preanestésica , Distribución AleatoriaRESUMEN
Formiminotransferase-cyclodeaminase is stabilized and activated approx. 40% in the presence of low concentrations (equal or less than 0.2%) of Triton X-100, possibly because the average hydrophobicity (1.10 kcal per residue) and the frequency of large non-polar side-chains (0.34) of this protein are both somewhat higher than average. This stabilization enabled us to develop a new purification procedure for the enzyme using chromatography on Matrex Gel Orange A and heparin-Sepharose columns in the presence of Triton X-100. This procedure is easier, much more reproducible, and gives slightly higher yield than the previous method described by Drury, et al. Further investigations of the role of tetrahydropteroylpolyglutamates with formiminotransferase-cyclodeaminase reveal that the use of polyglutamylated folate substrates does not change the mechanism of the transferase reaction, but decreases the K(m) for formininoglutamate, the second substrate, more than 10-fold, bringing it closer to the expected physiological concentration.
Asunto(s)
Amoníaco-Liasas/aislamiento & purificación , Ácido Fólico/análogos & derivados , Hígado/enzimología , Ácidos Pteroilpoliglutámicos/metabolismo , Aminoácidos/análisis , Amoníaco-Liasas/análisis , Amoníaco-Liasas/antagonistas & inhibidores , Amoníaco-Liasas/metabolismo , Animales , Cromatografía en Gel , Ácido Formiminoglutámico/metabolismo , Glutamatos/farmacología , Ácido Glutámico , Cinética , Peso Molecular , PorcinosRESUMEN
The effects of severe vitamin A deficiency (liver retinol less than 2 micrograms/g) on hepatic folate metabolism in rats were studied. The oxidation of a [ring-2-14C] histidine load or a [14C]formate load to 14CO2 was significantly depressed in vitamin A-deficient rats and those given histidine also excreted more urinary formiminoglutamic acid (FiGlu) than pair-fed controls. The increase in FiGlu excretion was not due to augmented production from histidine, implicating an impairment of FiGlu catabolism. FiGlu formiminotransferase activity was unaltered in vitamin A-deficient rats, but hepatic tetrahydrofolic acid (THF) concentration was decreased by 58% in vitamin A-deficient rats given a histidine load while 5-methyl-THF concentration was increased by 39%. Formyl-THF and total folate levels were similar to controls. A redistribution of folate coenzymes was not found in vitamin A-deficient rats not force fed histidine. A 43% decrease in 10-formyl-THF dehydrogenase activity, which generates both THF and the 14CO2 from the labeled substrates, and an 81% increase in 5,10-methylene-THF reductase activity, which generates 5-methyl-THF, were found in vitamin A-deficient rats. It appears that the production of severe vitamin A deficiency results in selective changes in the activities of hepatic folate-dependent enzymes, so that when a load of a one-carbon donor is given, THF concentration decreases and metabolism of the load is impaired.
Asunto(s)
Ácido Fólico/metabolismo , Hígado/metabolismo , Deficiencia de Vitamina A/metabolismo , Animales , Diterpenos , Ácido Formiminoglutámico/metabolismo , Histidina/administración & dosificación , Histidina/metabolismo , Histidina Amoníaco-Liasa/metabolismo , Masculino , Ratas , Ratas Endogámicas , Ésteres de Retinilo , S-Adenosilhomocisteína/análisis , S-Adenosilmetionina/análisis , Urocanato Hidratasa/metabolismo , Vitamina A/análogos & derivados , Vitamina A/metabolismo , Vitamina A/farmacologíaRESUMEN
The studies discussed in this review support the view that biochemical and clinical symptoms common to both folate and vitamin B12 deficiency are due to the induction of a functional folate deficiency, which in turn is induced by cobalamin deprivation. The interrelationship between these two vitamins is best explained by the methyl trap hypothesis stating that vitamin B12 deficiency can lead to lowered levels of methionine synthetase, which results in a functional folate deficiency by trapping an increased proportion of folate as the 5-methyl derivative. In addition, as 5-methyl-H4PteGlu is a poor substrate for folylpolyglutamate synthetase, there is a decreased synthesis of folylpolyglutamates and consequently a decreased retention of folates by tissues. The real folate deficiency that ensues because of decreased tissue folate levels is probably as important physiologically as the functional deficiency caused by the methyl trap. The sparing effect of methionine can be explained by adenosylmethionine inhibition of methylenetetrahydrofolate reductase, which would prevent the buildup of 5-methyl-H4PteGlun. A deficiency in vitamin B12 would not, in itself, be sufficient to cause a disturbance in folate metabolism. The deficiency would have to result in lowered methyltransferase levels before any such disturbance would be manifest.
Asunto(s)
Ácido Fólico/metabolismo , Vitamina B 12/metabolismo , 5-Metiltetrahidrofolato-Homocisteína S-Metiltransferasa/metabolismo , Aminoácidos/metabolismo , Animales , Médula Ósea/metabolismo , Ácido Fólico/sangre , Ácido Fólico/farmacología , Deficiencia de Ácido Fólico/patología , Formiatos/metabolismo , Ácido Formiminoglutámico/metabolismo , Histidina/metabolismo , Homeostasis , Humanos , Hígado/metabolismo , Megaloblastos/patología , Metionina/biosíntesis , Metionina/farmacología , Metilación , Óxido Nitroso/farmacología , Péptido Sintasas/metabolismo , Ácidos Pteroilpoliglutámicos/metabolismo , Purinas/biosíntesis , Serina/metabolismo , Timidina Monofosfato/biosíntesis , Timidilato Sintasa/metabolismo , Glándula Tiroides/fisiología , Vitamina B 12/farmacología , Deficiencia de Vitamina B 12/patologíaRESUMEN
Although folic acid is known to be involved in the pathways of purine metabolism, the precise changes brought about in purine synthesis, reutilization, pool sizes, and ratios by experimental folate deficiency are not clear. Consequently, these aspects of purine metabolism were measured in hepatocytes from control and folate-deficient rats fed an amino acid diet with and without folic acid, respectively. Purine synthesis and reutilization were measured as the rates of incorporation of [U-14C]glycine and [G-3H]hypoxanthine, respectively, into the adenine and guanine pools of freshly isolated hepatocytes after a 3-hour incubation in folate-free, as well as folate- and/or thymidine-supplemented culture media. Hepatocytes from folate-deficient rats had the same rates of purine synthesis as those from control rats. Purine reutilization, purine pool sizes, and the adenine:guanine ratios were lower in hepatocytes from deficient compared with control rats. Purine synthesis was increased when folic acid or thymidine was added to the culture medium. Although hepatocytes from folate-deficient rats had a lower rate of purine reutilization compared with those from control rats, the reutilization rates did not respond to the addition of folic acid or thymidine to the culture medium. The data suggest that purine synthesis was not impaired but purine reutilization was diminished in folate deficiency. Thymidine was as effective as folic acid in stimulating purine synthesis in both control and folate-deficient hepatocytes.
Asunto(s)
Deficiencia de Ácido Fólico/metabolismo , Hígado/metabolismo , Purinas/biosíntesis , Animales , Peso Corporal , Ácido Formiminoglutámico/metabolismo , Hipoxantina , Hipoxantinas/metabolismo , Recuento de Leucocitos , Masculino , Ratas , Ratas EndogámicasRESUMEN
A growth technique that allows strains of Streptomyces coelicolor to grow dispersed in defined liquid medium has been devised and used to determine the pathway of histidine degradation by S. coelicolor. Enzymic, chromatographic and stoichiometric analyses indicated that histidine is dissimilated via N-formyl-L-glutamic acid. The enzymes for histidine utilization (hut) are induced when histidine or urocanate is included in the culture medium. Biochemical evidence suggested that urocanate, or a further metabolite, is the physiological inducer. Three hut mutants were isolated and characterized. Two of the mutants exhibit an uninducible phenotype, whereas the third mutant appears to be defective in the structural gene for formiminoglutamate iminohydrolase. Haploid recombinant analysis was employed to locate all three mutations in the left empty region of the chromosomal map.
Asunto(s)
Proteínas Bacterianas , Histidina/metabolismo , Streptomyces/metabolismo , Amidohidrolasas/biosíntesis , Mapeo Cromosómico , Cruzamientos Genéticos , Medios de Cultivo , Inducción Enzimática , Ácido Formiminoglutámico/metabolismo , Histidina Amoníaco-Liasa/biosíntesis , Hidrolasas/biosíntesis , Hidrolasas/metabolismo , Mutación , Streptomyces/genética , Streptomyces/crecimiento & desarrollo , Urocanato Hidratasa/biosíntesis , Ácido Urocánico/metabolismoAsunto(s)
Alcoholismo/metabolismo , Ácido Fólico/metabolismo , Vitamina B 12/metabolismo , Alcoholismo/complicaciones , Animales , Cerveza , Células de la Médula Ósea , Eritropoyesis , Ácido Fólico/uso terapéutico , Deficiencia de Ácido Fólico , Ácido Formiminoglutámico/metabolismo , Humanos , Absorción Intestinal , Hepatopatías Alcohólicas/metabolismo , Trastornos Nutricionales/complicaciones , Enfermedades del Sistema Nervioso Periférico/metabolismo , Psicosis Alcohólicas/metabolismo , Tetrahidrofolatos/metabolismo , Trombocitopenia/metabolismo , Vitamina B 12/uso terapéutico , Deficiencia de Vitamina B 12 , VinoAsunto(s)
Errores Innatos del Metabolismo de los Aminoácidos/diagnóstico , Amoníaco-Liasas/deficiencia , Histidina Amoníaco-Liasa/deficiencia , Histidina/sangre , Adolescente , Adulto , Errores Innatos del Metabolismo de los Aminoácidos/epidemiología , Errores Innatos del Metabolismo de los Aminoácidos/genética , Errores Innatos del Metabolismo de los Aminoácidos/metabolismo , Animales , Niño , Preescolar , Femenino , Ácido Formiminoglutámico/metabolismo , Histidina Amoníaco-Liasa/genética , Humanos , Lactante , Recién Nacido , Discapacidad Intelectual/etiología , Tamizaje Masivo , Polimorfismo Genético , Embarazo , Trastornos del Habla/etiología , Ácido Urocánico/metabolismoAsunto(s)
Glucosafosfato Deshidrogenasa/efectos de la radiación , Pentosafosfatos/efectos de la radiación , Traumatismos por Radiación/metabolismo , Animales , Eritrocitos/metabolismo , Eritrocitos/efectos de la radiación , Ácido Formiminoglutámico/metabolismo , Ácido Formiminoglutámico/efectos de la radiación , Glucosafosfato Deshidrogenasa/metabolismo , Humanos , Masculino , NAD/efectos de la radiación , NADP/efectos de la radiación , Pentosafosfatos/metabolismo , Fosfogluconato Deshidrogenasa/metabolismo , Ratas , Tetrahidrofolatos/metabolismo , Tetrahidrofolatos/efectos de la radiación , Transcetolasa/metabolismoRESUMEN
1. Kinetic aspects of the reaction between crystalline bovine liver glutamate dehydrogenase and formiminoglutamate were investigated to establish the conditions under which the latter may interfere with the assay of glutamate by using glutamate dehydrogenase and to explain why formiminoglutamate accumulates in vivo after histidine loading, although it can react with glutamate dehydrogenase. The Km and Vmax. values were compared with those of the enzyme reacting with glutamate. At pH 7.4 Km for formiminoglutamate was much higher and Vmax. much lower than the values for glutamate. 2. The equilibrium constant at pH 7.0 was 0.017 micrometer with formiminoglutamate, i.e. about one two-hundredths that with glutamate. 3. In vivo the interaction between glutamate dehydrogenase and formiminoglutamate is minimal even when the concentration of the latter in the liver is greatly raised, as in cobalamine or folate deficiency after histidine loading. 4. At pH 9.3, i.e. under the conditions for the assay of glutamate by glutamate dehydrogenase, formiminoglutamate reacts readily with the enzyme.
Asunto(s)
Ácido Formiminoglutámico/metabolismo , Glutamato Deshidrogenasa/metabolismo , Glutaratos/metabolismo , Hígado/enzimología , Animales , Bovinos , Glutamato Deshidrogenasa/antagonistas & inhibidores , Glutamatos/metabolismo , Cinética , NAD/metabolismoAsunto(s)
Errores Innatos del Metabolismo de los Aminoácidos/terapia , Ácido Formiminoglutámico/metabolismo , Glutaratos/metabolismo , Transferasas/deficiencia , Animales , Niño , Inducción Enzimática , Ácido Formiminoglutámico/orina , Humanos , Hígado/enzimología , Masculino , Metionina/uso terapéutico , Ratas , Tetrahidrofolatos , Transferasas/metabolismo , Deficiencia de Vitamina B 12/orinaRESUMEN
Megaloblastic anaemia is due to a derangement of DNA synthesis caused by insufficient supply of one or other of the four deoxyribonucleoside triphosphate (dNTP) precursors of DNA synthesis or by direct inhibition of one or other DNA polymerase. Reduced supply of the pyrimidine deoxythymidine triphosphate (dTTP) may be caused by folate or vitamin B12 deficiencies or by the action of dihydrofolate reductase inhibitors (e.g. methotrexate, pyrimethamine or trimethoprim), all of which cause reduced supply of the coenzyme 5, 10 methylene tetrahydrofolate (pentaglutamate) needed for thymidylate synthetase. Reduced dTTP supply may also be caused by direct inhibition of thymidylate synthetase by 5-fluorouracil. Reduced supply of both purines, deoxyadenosine triphosphate (dATP) and deoxyguanosine triphosphate (dGTP), may be caused by hydroxyurea, 6-mercaptopurine (and probably by another purine antagonist azaserine), whilst reduced supply of both pyrimidine DNA precursors, dTTP and dCTP (deoxycytidine triphosphate) may be due to inherited orotic aciduria or to treatment with azauridine. Cytosine arabinoside directly inhibits DNA polymerase. DNA replication is a discontinuous process and a number of enzymes are concerned with different aspects of the process. The parental strands partly unwind and a large number of initiation points or origins are activated on both strands. A primer RNA is first synthesised using the parental strand of DNA as template. Fragments of new DNA are then synthesised on the parental DNA template, starting at the RNA primer, under the action of one or other DNA polymerase (probably gamma). The RNA primer is then removed and the gap left is filled by further DNA synthesis under the action of a different DNA polymerase (probably alpha). The fragments of new DNA are joined to give newly synthesised stretches of DNA (replicons) which are then liigated together to form bulk DNA of enormous molecular weight. It is suggested here that reduced supply of one or other of the four deoxyribonucleoside triphosphate (dNTP) during the 'S' phase of the cell cycle (due to vitamin B12 or folate deficiency, drug treatment or other congenital or acquired abnormality in synthesis of the dNTP) impairs the cell's ability to elongate newly initiated DNA fragments by preventing gap-filling, the polymerase needed for gap-filling requiring substantially greater concentrations of the deoxyribonucleoside triphosphates than the polymerase involved in chain initiation. Cytosine arabinoside, which also may cause megaloblastosis, may affect principally the synthesis of new DNA fragments. Since active protein synthesis is needed for the cell to enter the S phase and RNA synthesis is needed to prime new DNA synthesis, megaloblastic anaemia may be expected to occur only when DNA synthesis is inhibited but protein and RNA synthesis are relatively unimpaired...