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1.
Nature ; 613(7945): 783-789, 2023 01.
Artículo en Inglés | MEDLINE | ID: mdl-36631609

RESUMEN

Efficient and accurate termination is required for gene transcription in all living organisms1,2. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway3,4-called intrinsic termination transcription in bacteria-in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of Escherichia coli transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.


Asunto(s)
ADN Bacteriano , ARN Polimerasas Dirigidas por ADN , Escherichia coli , ARN Bacteriano , Terminación de la Transcripción Genética , Microscopía por Crioelectrón , ARN Polimerasas Dirigidas por ADN/química , ARN Polimerasas Dirigidas por ADN/metabolismo , ARN Polimerasas Dirigidas por ADN/ultraestructura , Escherichia coli/química , Escherichia coli/genética , Escherichia coli/metabolismo , Escherichia coli/ultraestructura , ARN Bacteriano/química , ARN Bacteriano/genética , ARN Bacteriano/metabolismo , ARN Bacteriano/ultraestructura , Regiones Terminadoras Genéticas/genética , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , ADN Bacteriano/ultraestructura
2.
FEMS Microbiol Rev ; 45(2)2021 03 16.
Artículo en Inglés | MEDLINE | ID: mdl-33016325

RESUMEN

The spatiotemporal regulation of gene expression plays an essential role in many biological processes. Recently, several imaging-based RNA labeling and detection methods, both in fixed and live cells, were developed and now enable the study of transcript abundance, localization and dynamics. Here, we review the main single-cell techniques for RNA visualization with fluorescence microscopy and describe their applications in bacteria.


Asunto(s)
Bacterias/ultraestructura , Microscopía Fluorescente , ARN Bacteriano/ultraestructura , Imagen Óptica
3.
Elife ; 92020 09 14.
Artículo en Inglés | MEDLINE | ID: mdl-32924932

RESUMEN

Using cryo-electron microscopy (cryo-EM), we determined the structure of the Escherichia coli 70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous positioning of protein and RNA residues, their detailed chemical interactions, and chemical modifications. Notable features include the first examples of isopeptide and thioamide backbone substitutions in ribosomal proteins, the former likely conserved in all domains of life. The maps also reveal extensive solvation of the small (30S) ribosomal subunit, and interactions with A-site and P-site tRNAs, mRNA, and the antibiotic paromomycin. The maps and models of the bacterial ribosome presented here now allow a deeper phylogenetic analysis of ribosomal components including structural conservation to the level of solvation. The high quality of the maps should enable future structural analyses of the chemical basis for translation and aid the development of robust tools for cryo-EM structure modeling and refinement.


Inside cells, proteins are produced by complex molecular machines called ribosomes. Techniques that allow scientists to visualize ribosomes at the atomic level, such as cryogenic electron microscopy (cryo-EM), help shed light on the structure of these molecular machines, revealing details of how they build proteins. Understanding how ribosomes work has many benefits, including the development of new antibiotics that can kill bacteria without affecting animal cells. Watson et al. used cryo-EM techniques with increased resolution to examine the ribosomes of the bacterium Escherichia coli in a higher level of detail than has been seen before. The results revealed two chemical modifications in proteins that form the ribosome that had not been observed in ribosomes previously. Additionally, a protein segment with a previously undescribed structure was identified close to the site where the ribosome reads the genetic instructions needed to make proteins. Further genetic analyses suggested these structures are in many related species, and may play important roles in how the ribosome works. Watson et al. were also able to see how paromomycin, an antibiotic used to treat parasitic infections, is positioned in the ribosome. The antibiotic interacts with a site near where the genetic code is read out, which might explain why certain changes to the antibiotic can interfere with its potency. Finally, the new ribosome structure reveals thousands of water molecules and metal ions that help keep the ribosome together as it produces proteins. This study shows the value of advances in cryo-EM technology and illustrates the importance of applying these techniques to other cell components. The results also reveal details of the ribosome useful for further research into this essential molecular machine.


Asunto(s)
Proteínas Bacterianas/ultraestructura , Escherichia coli/ultraestructura , ARN Bacteriano/ultraestructura , Proteínas Ribosómicas/ultraestructura , Ribosomas/ultraestructura , Microscopía por Crioelectrón , ARN Mensajero/ultraestructura , ARN de Transferencia/ultraestructura
4.
Nat Commun ; 10(1): 5728, 2019 12 16.
Artículo en Inglés | MEDLINE | ID: mdl-31844059

RESUMEN

Riboswitches are metabolite-sensing, conserved domains located in non-coding regions of mRNA that are central to regulation of gene expression. Here we report the first three-dimensional structure of the recently discovered S-adenosyl-L-methionine responsive SAM-VI riboswitch. SAM-VI adopts a unique fold and ligand pocket that are distinct from all other known SAM riboswitch classes. The ligand binds to the junctional region with its adenine tightly intercalated and Hoogsteen base-paired. Furthermore, we reveal the ligand discrimination mode of SAM-VI by additional X-ray structures of this riboswitch bound to S-adenosyl-L-homocysteine and a synthetic ligand mimic, in combination with isothermal titration calorimetry and fluorescence spectroscopy to explore binding thermodynamics and kinetics. The structure is further evaluated by analysis of ligand binding to SAM-VI mutants. It thus provides a thorough basis for developing synthetic SAM cofactors for applications in chemical and synthetic RNA biology.


Asunto(s)
Bifidobacterium/genética , Modelos Moleculares , Conformación de Ácido Nucleico , ARN Bacteriano/ultraestructura , Riboswitch/genética , Cristalografía por Rayos X , Ligandos , ARN Bacteriano/genética , S-Adenosilmetionina/metabolismo
6.
Nat Struct Mol Biol ; 26(12): 1094-1105, 2019 12.
Artículo en Inglés | MEDLINE | ID: mdl-31740854

RESUMEN

Amino acid availability in Gram-positive bacteria is monitored by T-box riboswitches. T-boxes directly bind tRNAs, assess their aminoacylation state, and regulate the transcription or translation of downstream genes to maintain nutritional homeostasis. Here, we report cocrystal and cryo-EM structures of Geobacillus kaustophilus and Bacillus subtilis T-box-tRNA complexes, detailing their multivalent, exquisitely selective interactions. The T-box forms a U-shaped molecular vise that clamps the tRNA, captures its 3' end using an elaborate 'discriminator' structure, and interrogates its aminoacylation state using a steric filter fashioned from a wobble base pair. In the absence of aminoacylation, T-boxes clutch tRNAs and form a continuously stacked central spine, permitting transcriptional readthrough or translation initiation. A modeled aminoacyl disrupts tRNA-T-box stacking, severing the central spine and blocking gene expression. Our data establish a universal mechanism of amino acid sensing on tRNAs and gene regulation by T-box riboswitches and exemplify how higher-order RNA-RNA interactions achieve multivalency and specificity.


Asunto(s)
Aminoácidos/metabolismo , Bacillus subtilis/metabolismo , Geobacillus/metabolismo , ARN Bacteriano/metabolismo , ARN de Transferencia/metabolismo , Riboswitch , Aminoacilación , Bacillus subtilis/química , Microscopía por Crioelectrón , Cristalografía por Rayos X , Geobacillus/química , Modelos Moleculares , Conformación de Ácido Nucleico , ARN Bacteriano/química , ARN Bacteriano/ultraestructura , ARN de Transferencia/química , ARN de Transferencia/ultraestructura
7.
Mol Cell ; 73(2): 264-277.e5, 2019 01 17.
Artículo en Inglés | MEDLINE | ID: mdl-30503773

RESUMEN

Type ΙΙΙ CRISPR-Cas systems provide robust immunity against foreign RNA and DNA by sequence-specific RNase and target RNA-activated sequence-nonspecific DNase and RNase activities. We report on cryo-EM structures of Thermococcus onnurineus CsmcrRNA binary, CsmcrRNA-target RNA and CsmcrRNA-target RNAanti-tag ternary complexes in the 3.1 Å range. The topological features of the crRNA 5'-repeat tag explains the 5'-ruler mechanism for defining target cleavage sites, with accessibility of positions -2 to -5 within the 5'-repeat serving as sensors for avoidance of autoimmunity. The Csm3 thumb elements introduce periodic kinks in the crRNA-target RNA duplex, facilitating cleavage of the target RNA with 6-nt periodicity. Key Glu residues within a Csm1 loop segment of CsmcrRNA adopt a proposed autoinhibitory conformation suggestive of DNase activity regulation. These structural findings, complemented by mutational studies of key intermolecular contacts, provide insights into CsmcrRNA complex assembly, mechanisms underlying RNA targeting and site-specific periodic cleavage, regulation of DNase cleavage activity, and autoimmunity suppression.


Asunto(s)
Autoinmunidad , Proteínas Bacterianas/metabolismo , Proteínas Asociadas a CRISPR/metabolismo , Sistemas CRISPR-Cas , Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas , Desoxirribonucleasas/metabolismo , Estabilidad del ARN , ARN Bacteriano/metabolismo , Proteínas de Unión al ARN/metabolismo , Proteínas Bacterianas/genética , Proteínas Bacterianas/inmunología , Proteínas Bacterianas/ultraestructura , Proteínas Asociadas a CRISPR/genética , Proteínas Asociadas a CRISPR/inmunología , Proteínas Asociadas a CRISPR/ultraestructura , Sistemas CRISPR-Cas/genética , Sistemas CRISPR-Cas/inmunología , Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas/genética , Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas/inmunología , Microscopía por Crioelectrón , Desoxirribonucleasas/genética , Desoxirribonucleasas/inmunología , Desoxirribonucleasas/ultraestructura , Escherichia coli/enzimología , Escherichia coli/genética , Escherichia coli/inmunología , Regulación Bacteriana de la Expresión Génica , Modelos Moleculares , Complejos Multiproteicos , Mutación , Conformación de Ácido Nucleico , Conformación Proteica , ARN Bacteriano/genética , ARN Bacteriano/inmunología , ARN Bacteriano/ultraestructura , Proteínas de Unión al ARN/genética , Proteínas de Unión al ARN/inmunología , Proteínas de Unión al ARN/ultraestructura , Relación Estructura-Actividad , Thermococcus/enzimología , Thermococcus/genética , Thermococcus/inmunología
8.
Microbiol Spectr ; 6(5)2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-30191804

RESUMEN

Diverse mechanisms and functions of posttranscriptional regulation by small regulatory RNAs and RNA-binding proteins have been described in bacteria. In contrast, little is known about the spatial organization of RNAs in bacterial cells. In eukaryotes, subcellular localization and transport of RNAs play important roles in diverse physiological processes, such as embryonic patterning, asymmetric cell division, epithelial polarity, and neuronal plasticity. It is now clear that bacterial RNAs also can accumulate at distinct sites in the cell. However, due to the small size of bacterial cells, RNA localization and localization-associated functions are more challenging to study in bacterial cells, and the underlying molecular mechanisms of transcript localization are less understood. Here, we review the emerging examples of RNAs localized to specific subcellular locations in bacteria, with indications that subcellular localization of transcripts might be important for gene expression and regulatory processes. Diverse mechanisms for bacterial RNA localization have been suggested, including close association to their genomic site of transcription, or to the localizations of their protein products in translation-dependent or -independent processes. We also provide an overview of the state of the art of technologies to visualize and track bacterial RNAs, ranging from hybridization-based approaches in fixed cells to in vivo imaging approaches using fluorescent protein reporters and/or RNA aptamers in single living bacterial cells. We conclude with a discussion of open questions in the field and ongoing technological developments regarding RNA imaging in eukaryotic systems that might likewise provide novel insights into RNA localization in bacteria.


Asunto(s)
Imagen Molecular/métodos , Transporte de ARN , ARN Bacteriano/ultraestructura , Coloración y Etiquetado/métodos , Aptámeros de Nucleótidos/química , Bacterias/genética , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Colorantes Fluorescentes , Regulación Bacteriana de la Expresión Génica , Proteínas Fluorescentes Verdes , Hibridación Fluorescente in Situ/métodos , Microscopía Fluorescente , Transporte de Proteínas , Transporte de ARN/fisiología , ARN Bacteriano/genética , ARN Bacteriano/fisiología , Proteínas de Unión al ARN/fisiología , Proteínas Recombinantes de Fusión , Ribonucleasas , Transcripción Genética
9.
Mol Cell ; 70(5): 881-893.e3, 2018 06 07.
Artículo en Inglés | MEDLINE | ID: mdl-29883607

RESUMEN

The assembly of ribosomal subunits is an essential prerequisite for protein biosynthesis in all domains of life. Although biochemical and biophysical approaches have advanced our understanding of ribosome assembly, our mechanistic comprehension of this process is still limited. Here, we perform an in vitro reconstitution of the Escherichia coli 50S ribosomal subunit. Late reconstitution products were subjected to high-resolution cryo-electron microscopy and multiparticle refinement analysis to reconstruct five distinct precursors of the 50S subunit with 4.3-3.8 Å resolution. These assembly intermediates define a progressive maturation pathway culminating in a late assembly particle, whose structure is more than 96% identical to a mature 50S subunit. Our structures monitor the formation and stabilization of structural elements in a nascent particle in unprecedented detail and identify the maturation of the rRNA-based peptidyl transferase center as the final critical step along the 50S assembly pathway.


Asunto(s)
Escherichia coli/metabolismo , ARN Bacteriano/metabolismo , ARN Ribosómico 23S/metabolismo , Subunidades Ribosómicas Grandes Bacterianas/metabolismo , Microscopía por Crioelectrón , Escherichia coli/genética , Escherichia coli/ultraestructura , Modelos Moleculares , Conformación de Ácido Nucleico , Conformación Proteica , ARN Bacteriano/genética , ARN Bacteriano/ultraestructura , ARN Ribosómico 23S/genética , ARN Ribosómico 23S/ultraestructura , Subunidades Ribosómicas Grandes Bacterianas/genética , Subunidades Ribosómicas Grandes Bacterianas/ultraestructura , Relación Estructura-Actividad
10.
Cell ; 170(4): 714-726.e10, 2017 Aug 10.
Artículo en Inglés | MEDLINE | ID: mdl-28757251

RESUMEN

Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. To understand how Cas13a is activated to cleave RNA, we have determined the crystal structure of Leptotrichia buccalis (Lbu) Cas13a bound to crRNA and its target RNA, as well as the cryo-EM structure of the LbuCas13a-crRNA complex. The crRNA-target RNA duplex binds in a positively charged central channel of the nuclease (NUC) lobe, and Cas13a protein and crRNA undergo a significant conformational change upon target RNA binding. The guide-target RNA duplex formation triggers HEPN1 domain to move toward HEPN2 domain, activating the HEPN catalytic site of Cas13a protein, which subsequently cleaves both single-stranded target and collateral RNAs in a non-specific manner. These findings reveal how Cas13a of type VI CRISPR-Cas systems defend against RNA phages and set the stage for its development as a tool for RNA manipulation.


Asunto(s)
Proteínas Bacterianas/química , Proteínas Asociadas a CRISPR/química , Sistemas CRISPR-Cas , Leptotrichia/inmunología , Proteínas Bacterianas/ultraestructura , Secuencia de Bases , Proteínas Asociadas a CRISPR/ultraestructura , Leptotrichia/química , Leptotrichia/metabolismo , Leptotrichia/virología , Modelos Moleculares , Procesamiento Postranscripcional del ARN , ARN Bacteriano/química , ARN Bacteriano/genética , ARN Bacteriano/ultraestructura , ARN Guía de Kinetoplastida/química , ARN Guía de Kinetoplastida/genética , ARN Guía de Kinetoplastida/ultraestructura , ARN Viral/química , Difracción de Rayos X
11.
Biochem Biophys Res Commun ; 487(2): 268-273, 2017 05 27.
Artículo en Inglés | MEDLINE | ID: mdl-28412369

RESUMEN

Ribosomal protein S1 (RpsA), the largest 30S protein in ribosome, plays a significant role in translation and trans-translation. In Mycobacterium tuberculosis, the C-terminus of RpsA is known as tuberculosis drug target of pyrazinoic acid, which inhibits the interaction between MtRpsA and tmRNA in trans-translation. However, the molecular mechanism underlying the interaction of MtRpsA with tmRNA remains unknown. We herein analyzed the interaction of the C-terminal domain of MtRpsA with three RNA fragments poly(A), sMLD and pre-sMLD. NMR titration analysis revealed that the RNA binding sites on MtRpsACTD are mainly located in the ß2, ß3 and ß5 strands and the adjacent L3 loop of the S1 domain. Fluorescence experiments determined the MtRpsACTD binding to RNAs are in the micromolar affinity range. Sequence analysis also revealed conserved residues in the mapped RNA binding region. Residues L304, V305, G308, F310, H322, I323, R357 and I358 were verified to be the key residues influencing the interaction between MtRpsACTD and pre-sMLD. Molecular docking further confirmed that the poly(A)-like sequence and sMLD of tmRNA are all involved in the protein-RNA interaction, through charged interaction and hydrogen bonds. The results will be beneficial for designing new anti-tuberculosis drugs.


Asunto(s)
Simulación del Acoplamiento Molecular , Mycobacterium tuberculosis/metabolismo , ARN Bacteriano/química , ARN Bacteriano/metabolismo , Proteínas Ribosómicas/química , Proteínas Ribosómicas/metabolismo , Sitios de Unión , Modelos Químicos , Unión Proteica , Biosíntesis de Proteínas/fisiología , Conformación Proteica , ARN Bacteriano/ultraestructura , Proteínas Ribosómicas/ultraestructura , Relación Estructura-Actividad
12.
Cell ; 169(1): 47-57.e11, 2017 03 23.
Artículo en Inglés | MEDLINE | ID: mdl-28340349

RESUMEN

Genetic conflict between viruses and their hosts drives evolution and genetic innovation. Prokaryotes evolved CRISPR-mediated adaptive immune systems for protection from viral infection, and viruses have evolved diverse anti-CRISPR (Acr) proteins that subvert these immune systems. The adaptive immune system in Pseudomonas aeruginosa (type I-F) relies on a 350 kDa CRISPR RNA (crRNA)-guided surveillance complex (Csy complex) to bind foreign DNA and recruit a trans-acting nuclease for target degradation. Here, we report the cryo-electron microscopy (cryo-EM) structure of the Csy complex bound to two different Acr proteins, AcrF1 and AcrF2, at an average resolution of 3.4 Å. The structure explains the molecular mechanism for immune system suppression, and structure-guided mutations show that the Acr proteins bind to residues essential for crRNA-mediated detection of DNA. Collectively, these data provide a snapshot of an ongoing molecular arms race between viral suppressors and the immune system they target.


Asunto(s)
Bacteriófagos/química , Proteínas Asociadas a CRISPR/química , Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas , Pseudomonas aeruginosa/inmunología , Pseudomonas aeruginosa/virología , ARN Bacteriano/química , Proteínas Virales/química , Bacteriófagos/clasificación , Bacteriófagos/genética , Microscopía por Crioelectrón , Cristalografía por Rayos X , Vigilancia Inmunológica , Modelos Moleculares , Pseudomonas aeruginosa/genética , ARN Bacteriano/metabolismo , ARN Bacteriano/ultraestructura , Proteínas Virales/ultraestructura
13.
J Phycol ; 52(5): 761-773, 2016 10.
Artículo en Inglés | MEDLINE | ID: mdl-27262053

RESUMEN

New Zealand ephemeral wetlands are ecologically important, containing up to 12% of threatened native plant species and frequently exhibiting conspicuous cyanobacterial growth. In such environments, cyanobacteria and associated heterotrophs can influence primary production and nutrient cycling. Wetland communities, including bacteria, can be altered by increased nitrate and phosphate due to agricultural practices. We have characterized cyanobacteria from the Wairepo Kettleholes Conservation Area and their associated bacteria. Use of 16S rRNA amplicon sequencing identified several operational taxonomic units (OTUs) representing filamentous heterocystous and non-heterocystous cyanobacterial taxa. One Nostoc OTU that formed macroscopic colonies dominated the cyanobacterial community. A diverse bacterial community was associated with the Nostoc colonies, including a core microbiome of 39 OTUs. Identity of the core microbiome associated with macroscopic Nostoc colonies was not changed by the addition of nutrients. One OTU was highly represented in all Nostoc colonies (27.6%-42.6% of reads) and phylogenetic analyses identified this OTU as belonging to the genus Sphingomonas. Scanning electron microscopy showed the absence of heterotrophic bacteria within the Nostoc colony but revealed a diverse community associated with the colonies on the external surface.


Asunto(s)
Biodiversidad , Cianobacterias/fisiología , Microbiota/fisiología , Humedales , Cianobacterias/clasificación , Cianobacterias/genética , Microbiota/genética , Nueva Zelanda , Filogenia , ARN Bacteriano/genética , ARN Bacteriano/ultraestructura , ARN Ribosómico 16S/genética
14.
Nat Struct Mol Biol ; 23(6): 549-57, 2016 06.
Artículo en Inglés | MEDLINE | ID: mdl-27136327

RESUMEN

Bacterial group II introns are large catalytic RNAs related to nuclear spliceosomal introns and eukaryotic retrotransposons. They self-splice, yielding mature RNA, and integrate into DNA as retroelements. A fully active group II intron forms a ribonucleoprotein complex comprising the intron ribozyme and an intron-encoded protein that performs multiple activities including reverse transcription, in which intron RNA is copied into the DNA target. Here we report cryo-EM structures of an endogenously spliced Lactococcus lactis group IIA intron in its ribonucleoprotein complex form at 3.8-Å resolution and in its protein-depleted form at 4.5-Å resolution, revealing functional coordination of the intron RNA with the protein. Remarkably, the protein structure reveals a close relationship between the reverse transcriptase catalytic domain and telomerase, whereas the active splicing center resembles the spliceosomal Prp8 protein. These extraordinary similarities hint at intricate ancestral relationships and provide new insights into splicing and retromobility.


Asunto(s)
Proteínas Bacterianas/metabolismo , Lactococcus lactis/metabolismo , ARN Bacteriano/metabolismo , ARN Catalítico/metabolismo , ADN Polimerasa Dirigida por ARN/metabolismo , Ribonucleoproteínas/metabolismo , Proteínas Bacterianas/química , Dominio Catalítico , Microscopía por Crioelectrón , Lactococcus lactis/química , Lactococcus lactis/ultraestructura , Modelos Moleculares , Conformación de Ácido Nucleico , Conformación Proteica , ARN Bacteriano/química , ARN Bacteriano/ultraestructura , ARN Catalítico/química , ARN Catalítico/ultraestructura , ADN Polimerasa Dirigida por ARN/química , Ribonucleoproteínas/química , Ribonucleoproteínas/ultraestructura
15.
Proc Natl Acad Sci U S A ; 113(18): 4994-9, 2016 May 03.
Artículo en Inglés | MEDLINE | ID: mdl-27092003

RESUMEN

During translation, a plethora of protein factors bind to the ribosome and regulate protein synthesis. Many of those factors are guanosine triphosphatases (GTPases), proteins that catalyze the hydrolysis of guanosine 5'-triphosphate (GTP) to promote conformational changes. Despite numerous studies, the function of elongation factor 4 (EF-4/LepA), a highly conserved translational GTPase, has remained elusive. Here, we present the crystal structure at 2.6-Å resolution of the Thermus thermophilus 70S ribosome bound to EF-4 with a nonhydrolyzable GTP analog and A-, P-, and E-site tRNAs. The structure reveals the interactions of EF-4 with the A-site tRNA, including contacts between the C-terminal domain (CTD) of EF-4 and the acceptor helical stem of the tRNA. Remarkably, EF-4 induces a distortion of the A-site tRNA, allowing it to interact simultaneously with EF-4 and the decoding center of the ribosome. The structure provides insights into the tRNA-remodeling function of EF-4 on the ribosome and suggests that the displacement of the CCA-end of the A-site tRNA away from the peptidyl transferase center (PTC) is functionally significant.


Asunto(s)
Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/ultraestructura , Factores de Iniciación de Péptidos/química , Factores de Iniciación de Péptidos/ultraestructura , ARN Bacteriano/química , ARN Bacteriano/ultraestructura , ARN de Transferencia/química , ARN de Transferencia/ultraestructura , Sitios de Unión , Simulación por Computador , Simulación del Acoplamiento Molecular , Conformación de Ácido Nucleico , Unión Proteica , Conformación Proteica , Motivos de Unión al ARN , Proteínas de Unión al ARN/química , Proteínas de Unión al ARN/ultraestructura , Ribosomas
16.
Methods ; 103: 49-56, 2016 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-27016143

RESUMEN

The assembly of the Escherichia coli ribosome has been widely studied and characterized in vitro. Despite this, ribosome biogenesis in living cells is only partly understood because assembly is coupled with transcription, modification and processing of the pre-ribosomal RNA. We present a method for footprinting and isolating pre-rRNA as it is synthesized in E. coli cells. Pre-rRNA synthesis is synchronized by starvation, followed by nutrient upshift. RNA synthesized during outgrowth is metabolically labeled to facilitate isolation of recent transcripts. Combining this technique with two in vivo RNA probing methods, hydroxyl radical and DMS footprinting, allows the structure of nascent RNA to be probed over time. Together, these can be used to determine changes in the structures of ribosome assembly intermediates as they fold in vivo.


Asunto(s)
Radical Hidroxilo/química , ARN Bacteriano/ultraestructura , ARN Ribosómico/ultraestructura , Ribosomas/ultraestructura , Ésteres del Ácido Sulfúrico/química , Técnicas de Cultivo de Célula , Escherichia coli , Conformación de Ácido Nucleico , ARN Bacteriano/química , ARN Ribosómico/química , Ribosomas/química , Coloración y Etiquetado
17.
Sci Rep ; 5: 17295, 2015 Nov 27.
Artículo en Inglés | MEDLINE | ID: mdl-26612428

RESUMEN

Live cell RNA imaging using genetically encoded fluorescent labels is an important tool for monitoring RNA activities. A recently reported RNA aptamer-fluorogen system, the Spinach, in which an RNA aptamer binds and induces the fluorescence of a GFP-like 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) ligand, can be readily tagged to the RNA of interest. Although the aptamer-fluorogen system is sufficient for imaging highly abundant non-coding RNAs (tRNAs, rRNAs, etc.), it performs poorly for mRNA imaging due to low brightness. In addition, whether the aptamer-fluorogen system may perturb the native RNA characteristics has not been systematically characterized at the levels of RNA transcription, translation and degradation. To increase the brightness of these aptamer-fluorogen systems, we constructed and tested tandem arrays containing multiple Spinach aptamers (8-64 aptamer repeats). Such arrays enhanced the brightness of the tagged mRNA molecules by up to ~17 fold in living cells. Strong laser excitation with pulsed illumination further increased the imaging sensitivity of Spinach array-tagged RNAs. Moreover, transcriptional fusion to the Spinach array did not affect mRNA transcription, translation or degradation, indicating that aptamer arrays might be a generalizable labeling method for high-performance and low-perturbation live cell RNA imaging.


Asunto(s)
Aptámeros de Nucleótidos/química , Escherichia coli/genética , Imagen Molecular/métodos , ARN Bacteriano/ultraestructura , ARN Mensajero/ultraestructura , Spinacia oleracea/química , Aptámeros de Nucleótidos/síntesis química , ADN Polimerasa Dirigida por ADN/genética , ADN Polimerasa Dirigida por ADN/metabolismo , Escherichia coli/metabolismo , Escherichia coli/ultraestructura , Operón Lac , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Biosíntesis de Proteínas , ARN Bacteriano/genética , ARN Bacteriano/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Spinacia oleracea/metabolismo , Secuencias Repetidas en Tándem , Transcripción Genética , Proteína Fluorescente Roja
18.
Nature ; 520(7548): 567-70, 2015 Apr 23.
Artículo en Inglés | MEDLINE | ID: mdl-25707802

RESUMEN

Single particle electron cryomicroscopy (cryo-EM) has recently made significant progress in high-resolution structure determination of macromolecular complexes due to improvements in electron microscopic instrumentation and computational image analysis. However, cryo-EM structures can be highly non-uniform in local resolution and all structures available to date have been limited to resolutions above 3 Å. Here we present the cryo-EM structure of the 70S ribosome from Escherichia coli in complex with elongation factor Tu, aminoacyl-tRNA and the antibiotic kirromycin at 2.65-2.9 Å resolution using spherical aberration (Cs)-corrected cryo-EM. Overall, the cryo-EM reconstruction at 2.9 Å resolution is comparable to the best-resolved X-ray structure of the E. coli 70S ribosome (2.8 Å), but provides more detailed information (2.65 Å) at the functionally important ribosomal core. The cryo-EM map elucidates for the first time the structure of all 35 rRNA modifications in the bacterial ribosome, explaining their roles in fine-tuning ribosome structure and function and modulating the action of antibiotics. We also obtained atomic models for flexible parts of the ribosome such as ribosomal proteins L9 and L31. The refined cryo-EM-based model presents the currently most complete high-resolution structure of the E. coli ribosome, which demonstrates the power of cryo-EM in structure determination of large and dynamic macromolecular complexes.


Asunto(s)
Microscopía por Crioelectrón , Escherichia coli/química , Escherichia coli/ultraestructura , Factor Tu de Elongación Peptídica/química , Factor Tu de Elongación Peptídica/ultraestructura , Ribosomas/química , Ribosomas/ultraestructura , Antibacterianos/química , Antibacterianos/metabolismo , Microscopía por Crioelectrón/métodos , Ligandos , Modelos Moleculares , Factor Tu de Elongación Peptídica/metabolismo , Piridonas/química , Piridonas/metabolismo , ARN Bacteriano/química , ARN Bacteriano/metabolismo , ARN Bacteriano/ultraestructura , ARN Ribosómico/química , ARN Ribosómico/metabolismo , ARN Ribosómico/ultraestructura , ARN de Transferencia/química , ARN de Transferencia/metabolismo , ARN de Transferencia/ultraestructura , Ribosomas/metabolismo
19.
Cell ; 153(1): 166-77, 2013 Mar 28.
Artículo en Inglés | MEDLINE | ID: mdl-23540697

RESUMEN

Many bacteria contain an ortholog of the Ro autoantigen, a ring-shaped protein that binds noncoding RNAs (ncRNAs) called Y RNAs. In the only studied bacterium, Deinococcus radiodurans, the Ro ortholog Rsr functions in heat-stress-induced ribosomal RNA (rRNA) maturation and starvation-induced rRNA decay. However, the mechanism by which this conserved protein and its associated ncRNAs act has been obscure. We report that Rsr and the exoribonuclease polynucleotide phosphorylase (PNPase) form an RNA degradation machine that is scaffolded by Y RNA. Single-particle electron microscopy, followed by docking of atomic models into the reconstruction, suggests that Rsr channels single-stranded RNA into the PNPase cavity. Biochemical assays reveal that Rsr and Y RNA adapt PNPase for effective degradation of structured RNAs. A Ro ortholog and ncRNA also associate with PNPase in Salmonella Typhimurium. Our studies identify another ribonucleoprotein machine and demonstrate that ncRNA, by tethering a protein cofactor, can alter the substrate specificity of an enzyme.


Asunto(s)
Deinococcus/química , Complejo Multienzimático de Ribonucleasas del Exosoma/química , Estabilidad del ARN , ARN Bacteriano/química , ARN no Traducido/metabolismo , Ribonucleoproteínas/metabolismo , Salmonella typhimurium/metabolismo , Animales , Secuencia de Bases , Deinococcus/genética , Deinococcus/metabolismo , Complejo Multienzimático de Ribonucleasas del Exosoma/metabolismo , Datos de Secuencia Molecular , Polirribonucleótido Nucleotidiltransferasa/química , Polirribonucleótido Nucleotidiltransferasa/ultraestructura , ARN Bacteriano/ultraestructura , ARN no Traducido/ultraestructura , Ribonucleoproteínas/química , Ribonucleoproteínas/genética , Xenopus laevis/metabolismo
20.
Nature ; 485(7399): 526-9, 2012 May 06.
Artículo en Inglés | MEDLINE | ID: mdl-22622583

RESUMEN

Bacterial ribosomes stalled at the 3' end of malfunctioning messenger RNAs can be rescued by transfer-messenger RNA (tmRNA)-mediated trans-translation. The SmpB protein forms a complex with the tmRNA, and the transfer-RNA-like domain (TLD) of the tmRNA then enters the A site of the ribosome. Subsequently, the TLD-SmpB module is translocated to the P site, a process that is facilitated by the elongation factor EF-G, and translation is switched to the mRNA-like domain (MLD) of the tmRNA. Accurate loading of the MLD into the mRNA path is an unusual initiation mechanism. Despite various snapshots of different ribosome-tmRNA complexes at low to intermediate resolution, it is unclear how the large, highly structured tmRNA is translocated and how the MLD is loaded. Here we present a cryo-electron microscopy reconstruction of a fusidic-acid-stalled ribosomal 70S-tmRNA-SmpB-EF-G complex (carrying both of the large ligands, that is, EF-G and tmRNA) at 8.3 Å resolution. This post-translocational intermediate (TI(POST)) presents the TLD-SmpB module in an intrasubunit ap/P hybrid site and a tRNA(fMet) in an intrasubunit pe/E hybrid site. Conformational changes in the ribosome and tmRNA occur in the intersubunit space and on the solvent side. The key underlying event is a unique extra-large swivel movement of the 30S head, which is crucial for both tmRNA-SmpB translocation and MLD loading, thereby coupling translocation to MLD loading. This mechanism exemplifies the versatile, dynamic nature of the ribosome, and it shows that the conformational modes of the ribosome that normally drive canonical translation can also be used in a modified form to facilitate more complex tasks in specialized non-canonical pathways.


Asunto(s)
Escherichia coli/química , Factor G de Elongación Peptídica/metabolismo , Biosíntesis de Proteínas , ARN Bacteriano/química , ARN Bacteriano/metabolismo , Proteínas de Unión al ARN/metabolismo , Ribosomas/metabolismo , Secuencia de Bases , Microscopía por Crioelectrón , Ácido Fusídico/metabolismo , Ligandos , Modelos Moleculares , Conformación de Ácido Nucleico , Factor G de Elongación Peptídica/química , Factor G de Elongación Peptídica/ultraestructura , Unión Proteica , Conformación Proteica , ARN Bacteriano/genética , ARN Bacteriano/ultraestructura , Proteínas de Unión al ARN/química , Proteínas de Unión al ARN/ultraestructura , Subunidades Ribosómicas/química , Subunidades Ribosómicas/genética , Subunidades Ribosómicas/metabolismo , Subunidades Ribosómicas/ultraestructura , Ribosomas/química , Ribosomas/genética , Ribosomas/ultraestructura
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