RESUMEN
On the basis of N-(3-amino-4-methoxyphenyl)acrylamide scaffold, a series of novel compounds containing 3-substitutional-1-methyl-1H-indole, 2-substitutional pyrrole or thiophene moieties were synthesized and their in vitro antiproliferation activities against A549 and H1975 cell lines were evaluated. The results indicated that most of the compounds showed moderate to excellent antitumor activities. Especially, compounds 9a (A549 IC50 = 1.96 µM, H1975 IC50 = 0.095 µM), 17i (A549 IC50 = 4.17 µM, H1975 IC50 = 0.052 µM), 17j (A549 IC50 = 1.67 µM, H1975 IC50 = 0.061 µM) exhibited comparable antitumor activities and selectivity ratios compared to the positive control osimertinib (A549 IC50 = 2.91 µM, H1975 IC50 = 0.064 µM). In vitro inhibitory activities against EGFR kinases containing different mutations were also tested. Compound 17i showed remarkable inhibitory activity (with IC50 value of 1.7 nM) to EGFRL858R/T790M kinase and selectivity (22-folds compared to EGFRWT kinase). Furthermore, acridine orange/ethidium bromide (AO/EB) staining assay, cell apoptosis assay, cell cycle distribution assay and wound-healing assay of the compounds 9a and 17i were performed on H1975 cell line. The results showed dose-dependent activities of the induction of apoptosis, G0/G1-phase arrestation and inhibition of migration, which were similar to the positive control osimertinib. Additionally, molecular docking analysis was performed to seek the possible binding mode between the selected compounds (9a, 17i-17j) and EGFRL858R/T790M kinase. The results demonstrated that compound 17i is a promising candidate and worth further study.
Asunto(s)
Acrilamida/farmacología , Antineoplásicos/farmacología , Diseño de Fármacos , Inhibidores de Proteínas Quinasas/farmacología , Acrilamida/síntesis química , Acrilamida/química , Antineoplásicos/síntesis química , Antineoplásicos/química , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Ensayos de Selección de Medicamentos Antitumorales , Receptores ErbB/antagonistas & inhibidores , Receptores ErbB/metabolismo , Humanos , Estructura Molecular , Inhibidores de Proteínas Quinasas/síntesis química , Inhibidores de Proteínas Quinasas/química , Relación Estructura-ActividadRESUMEN
Hepatocellular carcinoma (HCC) is the most prevalent primary liver cancer and one of the leading causes of cancer associated death worldwide. This is due to the highly resistant nature of this malignancy and the lack of effective treatment options for advanced stage HCC patients. The hyperactivity of PI3K/Akt and Ras/Raf/MEK/ERK signaling pathways contribute to the cancer progression, survival, motility, and resistance mechanisms, and the interaction of these two pathways are responsible for the regulation of cancer cell growth and development. Therefore, it is vital to design and develop novel therapeutic options for HCC treatment targeting these hyperactive pathways. For this purpose, novel series of trans-indole-3-ylacrylamide derivatives originated from the lead compound, 3-(1H-indole-3-yl)-N-(3,4,5-trimethoxyphenyl)acrylamide, have been synthesized and analyzed for their bioactivity on cancer cells along with the lead compound. Based on the initial screening, the most potent compounds were selected to elucidate their effects on cellular signaling activity of HCC cell lines. Cell cycle analysis, immunofluorescence, and Western blot analysis revealed that lead compound and (E)-N-(4-tert-butylphenyl)-3-(1H-indole-3-yl)acrylamide induced cell cycle arrest at the G2/M phase, enhanced chromatin condensation and PARP-cleavage, addressing induction of apoptotic cell death. Additionally, these compounds decreased the activity of ERK signaling pathway, where phosphorylated ERK1/2 and c-Jun protein levels diminished significantly. Relevant to these findings, the lead compound was able to inhibit tubulin polymerization as well. To conclude, the novel trans-indole-3-ylacrylamide derivatives inhibit one of the critical pathways associated with HCC which results in cell cycle arrest and apoptosis in HCC cell lines.
Asunto(s)
Acrilamida/farmacología , Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Carcinoma Hepatocelular/tratamiento farmacológico , Neoplasias Hepáticas/tratamiento farmacológico , Acrilamida/síntesis química , Acrilamida/química , Antineoplásicos/síntesis química , Antineoplásicos/química , Carcinoma Hepatocelular/patología , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Neoplasias Hepáticas/patología , Estructura Molecular , Relación Estructura-ActividadRESUMEN
Microbial bile salt hydrolases (BSHs) found in the intestine catalyze the deconjugation of taurine- and glycine-linked bile salts produced in the liver. The resulting bile salts are biological detergents and are critical in aiding lipophilic nutrient digestion. Therefore, the activity of BSHs in the gut microbiome is directly linked to human metabolism and overall health. Bile salt metabolism has also been associated with disease phenotypes such as liver and colorectal cancer. In order to reshape the gut microbiome to optimize bile salt metabolism, tools to characterize and quantify these processes must exist to enable a much-improved understanding of how metabolism goes awry in the face of disease, and how it can be improved through an altered lifestyle and environment. Furthermore, it is necessary to attribute metabolic activity to specific members and BSHs within the microbiome. To this end, we have developed activity-based probes with two different reactive groups to target bile salt hydrolases. These probes bind similarly to the authentic bile salt substrates, and we demonstrate enzyme labeling of active bile salt hydrolases by using purified protein, cell lysates, and in human stool.
Asunto(s)
Acrilamida/química , Amidohidrolasas/metabolismo , Ácidos y Sales Biliares/metabolismo , Colorantes Fluorescentes/química , beta-Lactamas/química , Acrilamida/síntesis química , Acrilamida/metabolismo , Amidohidrolasas/química , Ácidos y Sales Biliares/química , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/metabolismo , Microbioma Gastrointestinal , Humanos , Hidrólisis , Estructura Molecular , beta-Lactamas/síntesis química , beta-Lactamas/metabolismoRESUMEN
As a continuation of our previous work, a series of new phenyl acrylamide derivatives (4Aa-g, 4Ba-t, 5 and 6a-c) were designed and synthesized as non-nucleoside anti-HBV agents. Among them, compound 4Bs could potently inhibit HBV DNA replication in wild-type and lamivudine (3TC)/entecavir resistant HBV mutant strains with IC50 values of 0.19 and 0.18 µM, respectively. Notably, the selective index value of 4Bs was above 526, indicating the favorable safety profile. Interestingly, unlike nucleoside analogue 3TC, 4Bs could significantly inhibit 3.5 kb pgRNA expression. Molecular docking study revealed that 4Bs could fit well into the dimer-dimer interface of HBV core protein by hydrophobic, π-π and H-bond interactions. Considering the potent anti-HBV activity, low toxicity and diverse anti-HBV mechanism from that of nucleoside anti-HBV agent 3TC, compound 4Bs might be a promising lead to develop novel non-nucleoside anti-HBV therapeutic agents, and warranted further investigation.
Asunto(s)
Acrilamida/síntesis química , Antivirales/síntesis química , Virus de la Hepatitis B/efectos de los fármacos , Hepatitis B/tratamiento farmacológico , Acrilamida/farmacología , Antivirales/farmacología , Diseño de Fármacos , Resistencia a Medicamentos/efectos de los fármacos , Regulación de la Expresión Génica/efectos de los fármacos , Guanina/análogos & derivados , Guanina/química , Guanina/metabolismo , Células Hep G2 , Humanos , Enlace de Hidrógeno , Interacciones Hidrofóbicas e Hidrofílicas , Lamivudine/farmacología , Simulación del Acoplamiento Molecular , Mutación , Unión Proteica , Conformación Proteica , Relación Estructura-Actividad , Replicación Viral/efectos de los fármacosRESUMEN
Mitogen-activated protein kinase kinase 7 (MAP2K7) in the c-Jun N-terminal kinase signal cascade is an attractive drug target for a variety of diseases. The selectivity of MAP2K7 inhibitors against off-target kinases is a major barrier in drug development. We report a crystal structure of MAP2K7 complexed with a potent covalent inhibitor bearing an acrylamide moiety as an electrophile, which discloses a structural basis for producing selective and potent MAP2K7 inhibitors.
Asunto(s)
Acrilamida/farmacología , MAP Quinasa Quinasa 7/antagonistas & inhibidores , Inhibidores de Proteínas Quinasas/farmacología , Acrilamida/síntesis química , Acrilamida/química , Relación Dosis-Respuesta a Droga , Humanos , MAP Quinasa Quinasa 7/metabolismo , Modelos Moleculares , Estructura Molecular , Inhibidores de Proteínas Quinasas/síntesis química , Inhibidores de Proteínas Quinasas/química , Relación Estructura-ActividadRESUMEN
In this work, 3-Phenyl-N-[4-(10,15,20-triphenyl-porphyrin-5-yl)-phenyl]- acrylamide (TPPCA) was synthesized for the determination of metronidazole (MTZ). It was found that the type of fluorescence quenching was static quenching determined by Stern-Volmer plot and UV absorption spectroscopy, and thermodynamic related parameters were also obtained. Furthermore, the corresponding measurement conditions: the acidity of the system, the type of surfactant, the concentration of TPPCA and the sequence of reagent addition were optimized. Under the optimal experimental conditions, the linear range of MTZ was determined to be 0.01-0.20⯵gâ¯mL-1, and the limit of detection (LOD) was 0.004⯵gâ¯mL-1. Importantly, this report provides a simple, fast, and sensitive probe for the determination of MTZ in pharmaceutical practice.
Asunto(s)
Acrilamida/química , Cetrimonio/química , Emulsiones/química , Metronidazol/análisis , Sondas Moleculares/química , Ácidos/química , Acrilamida/síntesis química , Calibración , Concentración de Iones de Hidrógeno , Cinética , Límite de Detección , Espectrometría de Fluorescencia , Espectrofotometría Ultravioleta , Tensoactivos/química , Termodinámica , Factores de TiempoRESUMEN
A series of novel biaryloxazolidinone derivatives containing amide and acrylamide structure were designed, synthesized and evaluated for their antibacterial activity. Most compounds generally exhibited potent antibacterial activity with MIC values of 1⯵g/mL against S. aureus, MRSA, MSSA, LREF and VRE pathogens, using linezolid and radezolid as positive controls. Compound 17 exhibited good antibacterial activity with MIC values of 0.5⯵g/mL against S. aureus, MRSA, MSSA and VRE and 0.25⯵g/mL against LREF. The results indicated that compound 17 might serve as a potential hit-compound for further investigation.
Asunto(s)
Acrilamida/síntesis química , Amidas/síntesis química , Antibacterianos/uso terapéutico , Bacterias Grampositivas/efectos de los fármacos , Antibacterianos/farmacología , Humanos , Estructura Molecular , Relación Estructura-ActividadRESUMEN
Porous glycopolymers, "glycomonoliths", were prepared by radical polymerization based on polymerization-induced phase separation with an acrylamide derivative of α-mannose, acrylamide and cross-linker in order to investigate protein adsorption and separation. The porous structure was induced by a porogenic alcohol. The pore diameter and surface area were controlled by the type of alcohol. The protein adsorption was measured in both batch and continuous flow systems. The glycomonoliths showed specific interaction with the sugar recognition protein of concanavalin A, and non-specific interaction to other proteins was negligible. The amount of protein adsorption to the materials was determined by the sugar density and the composition of the glycomonoliths. Fundamental knowledge regarding the glycomonoliths for protein separation was obtained.
Asunto(s)
Acrilamida/química , Concanavalina A/aislamiento & purificación , Glicoconjugados/química , Manosa/análogos & derivados , Membranas Artificiales , Acrilamida/síntesis química , Adsorción , Concanavalina A/análisis , Glicoconjugados/síntesis química , Manosa/síntesis química , Transición de Fase , Polimerizacion , PorosidadRESUMEN
Oxidative stress and inflammation are major causes of numerous life-threatening human diseases. In the present study, we synthesized a series of phenylacrylamide derivatives as novel anti-oxidant and anti-inflammatory agents. Biological evaluation showed that compound 6a could more potently protect HBZY-1 mesangial cells from H2O2-caused oxidative stress than positive controls resveratrol and sulforaphane by dose- and time-dependently impairing the ROS accumulation. Preliminary anti-oxidant mechanism studies indicated that compound 6a could activate Nrf2 and increase the protein and mRNA expression of downstream anti-oxidant enzymes, ie. NQO-1, HO-1, GCLM and GCLC. Notably, 6a could inhibit the production of NO and the activity of NF-κB in LPS-stimulated HBZY-1 mesangial cells, indicating its potential anti-inflammatory activity. Interestingly, both effects could be significantly attenuated by Nrf2 inhibitor TRG, HO-1 inhibitor ZnPP or GCL inhibitor BSO at non-toxic concentrations, confirming that the anti-oxidant and anti-inflammatory activity of 6a is related to the activation of Nrf2 signaling pathway. These results, together with the relatively safety profile, indicated that compound 6a could be a promising lead to develop novel anti-oxidant and anti-inflammatory agents, thus preventing diseases induced by oxidative stress and inflammation.
Asunto(s)
Acrilamida/farmacología , Antiinflamatorios no Esteroideos/farmacología , Antioxidantes/farmacología , Inflamación/tratamiento farmacológico , Acrilamida/síntesis química , Acrilamida/química , Antiinflamatorios no Esteroideos/síntesis química , Antiinflamatorios no Esteroideos/química , Antioxidantes/síntesis química , Antioxidantes/química , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Células HEK293 , Humanos , Peróxido de Hidrógeno/antagonistas & inhibidores , Peróxido de Hidrógeno/farmacología , Estructura Molecular , Estrés Oxidativo/efectos de los fármacos , Relación Estructura-ActividadRESUMEN
Molecularly imprinted polymers (MIPs) have potential as alternatives to antibodies in the diagnosis and treatment of disease. However, atomistic level knowledge of the prepolymerization process is limited that would facilitate rational design of more efficient MIPs. Accordingly, we have investigated using computation and experiment the protein-monomer binding interactions that may influence the desired specificity. Myoglobin was used as the target protein and five different acrylamide-based monomers were considered. Protein binding sites were predicted using SiteMap and binding free energies of monomers at each site were calculated using MM-GBSA. Statistical thermodynamic analysis and study of atomistic interactions facilitated rationalization of monomer performance in MIP rebinding studies (% rebind; imprinting factors). CD spectroscopy was used to determine monomer effects on myoglobin secondary structure, with all monomers except the smallest monomer (acrylamide) causing significant changes. A complex interplay between different protein-monomer binding effects and MIP efficacy was observed. Validation of hypotheses for key binding features was achieved by rational selection of two different comonomer MIP combinations that produced experimental results in agreement with predictions. The comonomer studies revealed that uniform, noncompetitive binding of monomers around a target protein is favorable. This study represents a step toward future rational in silico design of MIPs for proteins.
Asunto(s)
Acrilamida/química , Teoría Funcional de la Densidad , Impresión Molecular , Mioglobina/análisis , Polímeros/química , Teoría Cuántica , Acrilamida/síntesis química , Dicroismo Circular , Estructura Molecular , Polímeros/síntesis químicaRESUMEN
In a search for novel multifunctional anti-Alzheimer agents, a congeneric set of seventeen flavone-8-acrylamide derivatives (8aâq) were synthesized and evaluated for their cholinesterase inhibitory, antioxidant, neuroprotective and modulation of Aß aggregation activities. The target compounds showed effective and selective inhibitory activity against the AChE over BuChE. In addition, the target compounds also showed moderate anti-oxidant activity and strong neuroprotective capacities, and accelerated dosage-dependently the Aß aggregation. Also, we presented here a complete study on the interaction of 8a, 8d, 8e, 8h and 8i with AChE. Through fluorescence emission studies, the binding sites number found to be 1, binding constants were calculated as 2.04â¯×â¯104, 2.22â¯×â¯104, 1.18â¯×â¯104, 9.8â¯×â¯103 and 3.2â¯×â¯104 M-1 and free energy change as -5.83, -5.91, -5.51, -5.41 and -6.12â¯kcalâ¯M-1 at 25⯰C which were well agreed with the computational calculations indicating a strong binding affinity of flavones and AChE. Furthermore, the CD studies revealed that the secondary structure of AChE became partly unfolded upon binding with 8a, 8d, 8e, 8h and 8i.
Asunto(s)
Acetilcolinesterasa/metabolismo , Acrilamida/farmacología , Enfermedad de Alzheimer/tratamiento farmacológico , Péptidos beta-Amiloides/antagonistas & inhibidores , Inhibidores de la Colinesterasa/farmacología , Flavonas/farmacología , Fármacos Neuroprotectores/farmacología , Acrilamida/síntesis química , Acrilamida/química , Enfermedad de Alzheimer/metabolismo , Péptidos beta-Amiloides/metabolismo , Sitios de Unión/efectos de los fármacos , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Inhibidores de la Colinesterasa/síntesis química , Inhibidores de la Colinesterasa/química , Relación Dosis-Respuesta a Droga , Flavonas/síntesis química , Flavonas/química , Humanos , Peróxido de Hidrógeno/antagonistas & inhibidores , Peróxido de Hidrógeno/farmacología , Estructura Molecular , Fármacos Neuroprotectores/síntesis química , Fármacos Neuroprotectores/química , Agregado de Proteínas/efectos de los fármacos , Relación Estructura-Actividad , TermodinámicaRESUMEN
An efficient method for the coupling of N-alkylamines with silicon enolates to generate ß-amino carbonyl compounds is disclosed. These reactions proceed by activation of α-amino C-H bonds by B(C6F5)3, which likely generates a "frustrated" acid/base complex in the presence of large N-alkylamines. The transformation requires no external oxidant and releases hydrosilane as a byproduct. The utility of this method is demonstrated in the late-stage functionalization of bioactive molecules such as citalopram, atomoxetine, and fluoxetine.
Asunto(s)
Acrilamida/síntesis química , Aminas/química , Boranos/química , Hidrocarburos Fluorados/química , Oxidantes/química , Silicio/química , Acrilamida/química , Catálisis , Estructura MolecularRESUMEN
The aim of present study was to develop a pH responsive rate controlling polymer by acrylamide grafting onto pullulan. Grafting was performed using free radical induced microwave assisted irradiation technique using ceric ammonium nitrate as free radical inducer. Acrylamide grafted pullulan (Aam-g-pull) was characterized by Fourier transform infrared spectroscopy, solid state 13C nuclear magnetic resonance and field emission scanning electron microscopy. In vitro enzymatic degradation of Aam-g-pull showed degradation of 22.45% after 8â¯h with degradation rate constant (k) of 0.019â¯min-1. In vitro cytotoxicity test did not show cell viability below 80% on HepG2 cell line. Pirfenidone tablets were prepared by utilizing wet granulation method using Aam-g-pull as the only rate controlling polymer. The tablets were characterized in terms of in-process quality control parameters like weight variation, hardness, assay, and in vitro dissolution study. The dissolution study showed that the cumulative drug release in phosphate buffer pHâ¯6.8 (rel3 hâ¯=â¯44.12⯱â¯0.56%) got a significant jump as compared to the release in 0.1â¯N hydrochloric acid (rel2 hâ¯=â¯26.78⯱â¯0.23%), confirming the material to be pH responsive. Aam-g-pull can be used as pH responsive rate controlling polymer.
Asunto(s)
Sistemas de Liberación de Medicamentos , Glucanos/química , Glucanos/farmacología , Polímeros/química , Acrilamida/síntesis química , Acrilamida/química , Acrilamida/farmacología , Supervivencia Celular/efectos de los fármacos , Glucanos/síntesis química , Glucanos/ultraestructura , Células Hep G2 , Humanos , Microscopía Electrónica de Rastreo , Microesferas , Microondas , Polímeros/síntesis química , Polímeros/farmacología , Espectroscopía Infrarroja por Transformada de Fourier , Comprimidos/químicaRESUMEN
2-d-Acrylamide was synthesized via the 2-step procedure starting from acrylonitrile and deuterium oxide. This procedure affords 2-d-acrylamide in 99.9% chemical purity and 98.4% isotopic enrichment.
Asunto(s)
Acrilamida/química , Acrilamida/síntesis química , Acrilonitrilo/química , Técnicas de Química Sintética , Óxido de Deuterio/química , EstereoisomerismoRESUMEN
Cereals-based food is one of the major source of Maillard reaction products in the diet. Free amino acids and reducing sugars are considered to be the main precursors in the formation of these heat-induced compounds. In order to determine genetic resources with reduced potential for acrylamide formation, the content of sugars as well as free asparagine were analysed in a total of 30 cultivars of 10 varieties belonging to eight species (Triticum aestivum var. lutescens, T. aestivum var. alba, T. aestivum var. compactum, T. durum, T. spelta, T. dicoccum, Secale cereale, Hordeum vulgare var. nudum, Avena sativa var. nudum, and Zea mays var. indentata) grown at the same location in the 2015 growing season. Our results provide evidence of differences in the content of sugars and asparagine between and within species of small grain cereals and maize. The highest content of glucose, fructose and asparagine was found in cultivars of rye and hull-less oat. All maize varieties examined contained significantly higher amounts of non-reducing and total sugars (on average 1.25% and 2.36%, respectively) than small cereal grain species. Principal component analysis showed a high positive correlation between monoreducing sugars and asparagine in bread wheat, durum wheat and hull-less barley.
Asunto(s)
Acrilamida/análisis , Acrilamida/química , Asparagina/análisis , Carbohidratos/análisis , Carbohidratos/química , Grano Comestible/química , Análisis de los Alimentos , Acrilamida/síntesis química , Asparagina/química , Análisis de Componente PrincipalRESUMEN
The synthesis of chitosan (Chs) and chitin (Chi) copolymer and grafting of acrylamide (AAm) onto the synthesized copolymer have been carried out by chemical methods. The grafted copolymer was characterized by FTIR, SEM and XRD. The extracellular cutinase of Aspergillus sp. RL2Ct (E.C. 3.1.1.3) was purified to 4.46 fold with 16.1% yield using acetone precipitation and DEAE sepharose ion exchange chromatography. It was immobilized by adsorption on the grafted copolymer. The immobilized enzyme was found to be more stable then the free enzyme and has a good binding efficiency (78.8%) with the grafted copolymer. The kinetic parameters KM and Vmax for free and immobilized cutinase were found to be 0.55mM and 1410µmolmin-1mg-1 protein, 2.99mM and 996µmolmin-1mg-1 protein, respectively. The immobilized cutinase was recycled 64 times without considerable loss of activity. The matrix (Chs-co-Chi-g-poly(AAm)) prepared and cutinase immobilized on the matrix have potential applications in enzyme immobilization and organic synthesis respectively.
Asunto(s)
Acrilamida/química , Aspergillus/enzimología , Hidrolasas de Éster Carboxílico/química , Quitina/química , Quitosano/química , Enzimas Inmovilizadas/química , Acrilamida/síntesis química , Adsorción , Aspergillus/química , Aspergillus/metabolismo , Hidrolasas de Éster Carboxílico/síntesis química , Hidrolasas de Éster Carboxílico/metabolismo , Quitina/síntesis química , Quitosano/síntesis química , Estabilidad de Enzimas , Enzimas Inmovilizadas/metabolismo , PolimerizacionRESUMEN
NLRP3 inflammasome plays a key role in the intracellular activation of caspase-1, processing of pro-inflammatory interleukin-1ß (IL-1ß), and pyroptotic cell death cascade. The overactivation of NLRP3 is implicated in the pathogenesis of autoinflammatory diseases, known as cryopyrin-associated periodic syndromes (CAPS), and in the progression of several diseases, such as atherosclerosis, type-2 diabetes, gout, and Alzheimer's disease. In this study, the synthesis of acrylamide derivatives and their pharmaco-toxicological evaluation as potential inhibitors of NLRP3-dependent events was undertaken. Five hits were identified and evaluated for their efficiency in inhibiting IL-1ß release from different macrophage subtypes, including CAPS mutant macrophages. The most attractive hits were tested for their ability to inhibit NLRP3 ATPase activity on human recombinant NLRP3. This screening allowed the identification of 14, 2-(2-chlorobenzyl)-N-(4-sulfamoylphenethyl)acrylamide, which was able to concentration-dependently inhibit NLRP3 ATPase with an IC50 value of 74â µm. The putative binding pose of 14 in the ATPase domain of NLRP3 was also proposed.
Asunto(s)
Acrilamida/farmacología , Diseño de Fármacos , Inflamasomas/antagonistas & inhibidores , Proteína con Dominio Pirina 3 de la Familia NLR/antagonistas & inhibidores , Acrilamida/síntesis química , Acrilamida/química , Animales , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Relación Dosis-Respuesta a Droga , Humanos , Inflamasomas/genética , Interleucina-1beta/antagonistas & inhibidores , Interleucina-1beta/metabolismo , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Ratones , Ratones Endogámicos C57BL , Modelos Moleculares , Estructura Molecular , Proteína con Dominio Pirina 3 de la Familia NLR/deficiencia , Proteína con Dominio Pirina 3 de la Familia NLR/genética , Relación Estructura-ActividadRESUMEN
Mitigation of acrylamide formation and the functional properties of Maillard reaction products (MRPs) were investigated in a food model system. The system was composed of elements of mixtures and their combination including fructose, asparagine and different molecular weight chitosans. All solutions were heated, and then analyzed for acrylamide content, MRPs absorbance, pH, color, antioxidant capacity, antibacterial activity and kinematic viscosity. The fructose, asparagine and chitosan mixture had more MRPs compared to other mixtures. 1,1-Diphenyl-2-pricrylhydrazy (DPPH) radical scavenging activities, ferrous ion chelating abilities and reducing power results showed that all solutions containing a combination of two or three reactants had antioxidant capacities. Acrylamide content has a positive correlation with absorbance values at OD294 and OD420 but a negative correlation with the CIB L(∗) value of a solution (p<0.01). Experimental results evidenced that low molecular weight (50-190 kDa) chitosan can be used to mitigate the formation of acrylamide.
Asunto(s)
Acrilamida/síntesis química , Quitosano/química , Reacción de Maillard , Acrilamida/análisis , Antioxidantes/química , Asparagina/química , Quelantes/química , Quitosano/análisis , Fructosa/química , Calor , Peso Molecular , SolucionesRESUMEN
Homopolymerization of an optically active acrylamide having an amino acid moiety in the side chain, N-acryloyl-L-alanine (AAla) was carried out via atom transfer radical polymerization (ATRP) at room temperature using 2-hydroxyethyl-2'-methyl-2'-bromopropionate (HMB) or sodium-4-(bromomethyl)benzoate (SBB) as initiator in pure water, methanol/water mixture and pure methanol solvents. The polymerization reaction resulted in the optically active biocompatible amino acid-based homopolymer in good yield with narrow molecular weight distribution. The number average molecular weight increased with conversion and polydispersity was low. The structure and molecular weight of synthesized polymer were characterized by (1)H NMR, FT-IR spectroscopic techniques and size-exclusion chromatography.
Asunto(s)
Acrilamida/síntesis química , Alanina/análogos & derivados , Polímeros/síntesis química , Acrilamida/química , Alanina/síntesis química , Alanina/química , Resonancia Magnética Nuclear Biomolecular , Polimerizacion , Polímeros/química , Espectroscopía de Protones por Resonancia Magnética , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
As flocculant plays an important role in wastewater treatment, searching for high efficient and cost-effective flocculants has always become the challenge in chemical industry. In the current work, lignosulfonate-acrylamide-chitosan ternary copolymer was designed and prepared as a new kind of flocculant. The elemental analysis and structure characterization of FTIR and XRD showed that acrylamide successfully grafted onto the two natural polymers and amorphous macromolecules were formed. The natural polymers-based flocculant was water soluble and pH independent. As it had multiple functional groups from the raw materials, the amphoteric flocculant showed high color removal efficiency to anionic (acid blue 113, >95%), neutral (reactive black 5, >95%) and cationic dyes (methyl orange, >50%) in a wide range of flocculant dosage and pH windows. The ternary flocculant, based on lignosulfonate, chitosan, and acrylamide, might be a promising material in practical applications from the perspective of cost, source and performance.