RESUMEN
Heterogeneous oxidation by gas-phase oxidants is an important chemical transformation pathway of secondary organic aerosol (SOA) and plays an important role in controlling the abundance, properties, as well as climate and health impacts of aerosols. However, our knowledge on this heterogeneous chemistry remains inadequate. In this study, the heterogeneous oxidation of α-pinene ozonolysis SOA by hydroxyl (OH) radicals was investigated under both low and high relative humidity (RH) conditions, with an emphasis on the evolution of molecular composition of SOA and its RH dependence. It is found that the heterogeneous oxidation of SOA at an OH exposure level equivalent to 12 hr of atmospheric aging leads to particle mass loss of 60% at 25% RH and 95% at 90% RH. The heterogeneous oxidation strongly changes the molecular composition of SOA. The dimer-to-monomer signal ratios increase dramatically with rising OH exposure, in particular under high RH conditions, suggesting that aerosol water stimulates the reaction of monomers with OH radicals more than that of dimers. In addition, the typical SOA tracer compounds such as pinic acid, pinonic acid, hydroxy pinonic acid and dimer esters (e.g., C17H26O8 and C19H28O7) have lifetimes of several hours against heterogeneous OH oxidation under typical atmospheric conditions, which highlights the need for the consideration of their heterogeneous loss in the estimation of monoterpene SOA concentrations using tracer-based methods. Our study sheds lights on the heterogeneous oxidation chemistry of monoterpene SOA and would help to understand their evolution and impacts in the atmosphere.
Asunto(s)
Aerosoles , Contaminantes Atmosféricos , Monoterpenos Bicíclicos , Humedad , Radical Hidroxilo , Oxidación-Reducción , Aerosoles/química , Radical Hidroxilo/química , Monoterpenos Bicíclicos/química , Contaminantes Atmosféricos/química , Contaminantes Atmosféricos/análisis , Ozono/química , Modelos Químicos , Atmósfera/química , Monoterpenos/químicaRESUMEN
Ultrasonic humidifiers are commonly used in households to maintain indoor humidity and generate a large number of droplets or spray aerosols. However, there have been various health concerns associated with humidifier use, largely due to aerosols generated during operation. Here, we investigated the size distribution, chemical composition, and charged fraction of aerosol particles emitted from commercial ultrasonic humidifiers. Heavy metals in water used for humidifiers were found to be highly enriched in the ultrasonic humidifier aerosols (UHA), with the enrichment factors ranging from 102 to 107. This enrichment may pose health concerns for the building occupants, as UHA concentrations of up to 106 particles/cm3 or 3 mg/m3 were observed. Furthermore, approximately 90% of UHA were observed to be electrically charged, for the first time according to our knowledge. Based on this discovery, we proposed and tested a new method to remove UHA by using a simple electrical field. The designed electrical field in this work can efficiently remove 81.4% of UHA. Therefore, applying this electrical field could be an effective method to significantly reduce the health risks by UHA.
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Aerosoles , Humidificadores , Metales Pesados , Aerosoles/análisis , Metales Pesados/análisis , Contaminación del Aire Interior/prevención & control , Contaminación del Aire Interior/análisis , Contaminantes Atmosféricos/análisis , Ultrasonido , Monitoreo del Ambiente/métodosRESUMEN
Thermodynamic modeling is still the most widely used method to characterize aerosol acidity, a critical physicochemical property of atmospheric aerosols. However, it remains unclear whether gas-aerosol partitioning should be incorporated when thermodynamic models are employed to estimate the acidity of coarse particles. In this work, field measurements were conducted at a coastal city in northern China across three seasons, and covered wide ranges of temperature, relative humidity and NH3 concentrations. We examined the performance of different modes of ISORROPIA-II (a widely used aerosol thermodynamic model) in estimating aerosol acidity of coarse and fine particles. The M0 mode, which incorporates gas-phase data and runs the model in the forward mode, provided reasonable estimation of aerosol acidity for coarse and fine particles. Compared to M0, the M1 mode, which runs the model in the forward mode but does not include gas-phase data, may capture the general trend of aerosol acidity but underestimates pH for both coarse and fine particles; M2, which runs the model in the reverse mode, results in large errors in estimated aerosol pH for both coarse and fine particles and should not be used for aerosol acidity calculations. However, M1 significantly underestimates liquid water contents for both fine and coarse particles, while M2 provides reliable estimation of liquid water contents. In summary, our work highlights the importance of incorporating gas-aerosol partitioning when estimating coarse particle acidity, and thus may help improve our understanding of acidity of coarse particles.
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Aerosoles , Contaminantes Atmosféricos , Modelos Químicos , Termodinámica , Aerosoles/análisis , Aerosoles/química , Contaminantes Atmosféricos/química , Contaminantes Atmosféricos/análisis , China , Monitoreo del Ambiente/métodos , Material Particulado/química , Material Particulado/análisis , Concentración de Iones de Hidrógeno , Tamaño de la PartículaRESUMEN
To explore air contamination resulting from special biomass combustion and suspended dust in Lhasa, the present study focused on the size distribution and chemical characteristics of particulate matter (PM) emission resulting from 7 types of non-fossil pollution sources. We investigated the concentration and size distribution of trace elements from 7 pollution sources collected in Lhasa. Combining Lhasa's atmospheric particulate matter data, enrichment factors (EFs) have been calculated to examine the potential impact of those pollution sources on the atmosphere quality of Lhasa. The highest mass concentration of total elements of biomass combustion appeared at PM0.4, and the second highest concentration existed in the size fraction 0.4-1 µm; the higher proportion (12 %) of toxic metals was produced by biomass combustion. The elemental composition of suspended dust and atmospheric particulate matter was close (except for As and Cd); the highest concentration of elements was all noted in PM2.5-10 (PM3-10). Potassium was found to be one of the main biomass markers. The proportion of Cu in suspended dust is significantly lower than that of atmospheric particulate matter (0.53 % and 3.75 %), which indicates that there are other anthropogenic sources. The EFs analysis showed that the Cr, Cu, Zn, and Pb produced by biomass combustion were highly enriched (EFs > 100) in all particle sizes. The EFs of most trace elements increased with decreasing particle size, indicating the greater influence of humanfactors on smaller particles.
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Aerosoles , Contaminantes Atmosféricos , Polvo , Monitoreo del Ambiente , Tamaño de la Partícula , Material Particulado , Contaminantes Atmosféricos/análisis , Aerosoles/análisis , Material Particulado/análisis , Polvo/análisis , Oligoelementos/análisis , Contaminación del Aire/estadística & datos numéricos , Contaminación del Aire/análisis , China , Atmósfera/químicaRESUMEN
There are limited biosecurity measures directed at preventing airborne transmission of viruses in swine. The effectiveness of dust mitigation strategies such as oil sprinkling, to decrease risk of airborne virus transmission are unknown. Metagenomics and qPCR for common fecal viruses were used to hunt for a ubiquitous virus to serve as a proxy when evaluating the efficiency of mitigation strategies against airborne viral infectious agents. Air particles were collected from swine buildings using high-volume air samplers. Extracted DNA and RNA were used to perform specific RT-qPCR and qPCR and analyzed by high-throughput sequencing. Porcine astroviruses group 2 were common (from 102 to 105 genomic copies per cubic meter of air or gc/m3, 93% positivity) while no norovirus genogroup II was recovered from air samples. Porcine torque teno sus virus were detected by qPCR in low concentrations (from 101 to 102 gc/m3, 47% positivity). Among the identified viral families by metagenomics analysis, Herelleviridae, Microviridae, Myoviridae, Podoviridae, and Siphoviridae were dominant. The phage vB_AviM_AVP of Aerococcus was present in all air samples and a newly designed qPCR revealed between 101 and 105 gc/m3 among the samples taken for the present study (97% positivity) and banked samples from 5- and 15-year old studies (89% positivity). According to the present study, both the porcine astrovirus group 2 and the phage vB_AviM_AVP of Aerococcus could be proxy for airborne viruses of swine buildings.
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Microbiología del Aire , Monitoreo del Ambiente , Metagenómica , Animales , Porcinos , Monitoreo del Ambiente/métodos , Aerosoles/análisis , Virus/aislamiento & purificación , Contaminación del Aire Interior/análisis , Vivienda para AnimalesRESUMEN
Fine-mode aerosol optical depth (fAOD) is a vital proxy for the concentration of anthropogenic aerosols in the atmosphere. Currently, the limited data length and high uncertainty of the satellite-based data diminish the applicability of fAOD for climate research. Here, we propose a novel pretrained deep learning framework that can extract information underlying each satellite pixel and use it to create new latent features that can be employed for improving retrieval accuracy in regions without in situ data. With the proposed model, we developed a new global fAOD (at 0.5 µm) data from 2001 to 2020, resulting in a 10% improvement in the overall correlation coefficient (R) during site-based independent validation and a 15% enhancement in non-AERONET site areas validation. Over the past two decades, there has been a noticeable downward trend in global fAOD (-1.39 × 10-3/year). Compared to the general deep-learning model, our method reduces the global trend's previously overestimated magnitude by 7% per year. China has experienced the most significant decline (-5.07 × 10-3/year), which is 3 times greater than the global trend. Conversely, India has shown a significant increase (7.86 × 10-4/year). This study bridges the gap between sparse in situ observations and abundant satellite measurements, thereby improving predictive models for global patterns of fAOD and other climate factors.
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Aerosoles , Aprendizaje Profundo , Atmósfera/química , Monitoreo del Ambiente/métodos , Imágenes SatelitalesAsunto(s)
Aerosoles , Betametasona , Calcitriol , Fármacos Dermatológicos , Combinación de Medicamentos , Enfermedades de la Uña , Psoriasis , Humanos , Psoriasis/tratamiento farmacológico , Calcitriol/análogos & derivados , Calcitriol/administración & dosificación , Calcitriol/uso terapéutico , Betametasona/administración & dosificación , Betametasona/análogos & derivados , Betametasona/uso terapéutico , Fármacos Dermatológicos/administración & dosificación , Fármacos Dermatológicos/uso terapéutico , Enfermedades de la Uña/tratamiento farmacológico , Masculino , Femenino , Urea/administración & dosificación , Urea/uso terapéutico , Urea/análogos & derivados , Adulto , Resultado del Tratamiento , Persona de Mediana EdadRESUMEN
Because of the increasing popularity of e-cigarettes, monitoring the e-cigarette market has become important for national health authorities to guarantee safety and quality. In the EU, the Tobacco Products Directive requires emission studies for e-cigarette products. The absence of industry guidelines for studying these emissions and the lack of proper validation in the literature led us to develop and validate a method using the total error approach for the determination of nicotine in e-cigarette aerosols. A commercial vaping device was used to generate aerosols, which were then collected on Cambridge filter pads and measured for nicotine concentration by UHPLC-DAD after extraction. The method was successfully validated by generating accuracy profiles, which show that the ß-expectation tolerance intervals remained below the acceptance limits of ±20%. Within-run repeatability and intermediate precision were considered acceptable since the highest RSD value obtained was below 5%. The method was applied to 15 commercial e-liquids. A complete validation of a method for the analysis of e-cigarette emissions is presented, including several parameters that impact the accuracy and reproducibility. Similar systematic approaches for method development and validation could be used for other e-cigarette emission analysis methods to ensure the reliability of the measurements.
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Aerosoles , Sistemas Electrónicos de Liberación de Nicotina , Nicotina , Aerosoles/análisis , Nicotina/análisis , Reproducibilidad de los Resultados , Cromatografía Líquida de Alta Presión/métodos , VapeoRESUMEN
To investigate the carcinogenicity of anatase-type nano-titanium dioxide (aNTiO2), F344/DuCrlCrlj rats were exposed to aNTiO2 aerosol at concentrations of 0, 0.5, 2, and 8 mg/m3. The rats were divided into 2 groups: carcinogenicity study groups were exposed for two years, and satellite study groups were exposed for one year followed by recovery for 1 day, 26 weeks, and 52 weeks after the end of exposure. In the carcinogenicity groups, bronchiolo-alveolar carcinomas were observed in two 8 mg/m3-exposed males, showing an increasing trend by Peto's test. However, this incidence was at the upper limit of JBRC's historical control data. Bronchiolo-alveolar adenomas were observed in 1, 2, 3, and 4 rats of the 0, 0.5, 2, and 8 mg/m3-exposed females and were not statistically significant. However, the incidence in the 8 mg/m3-exposed females exceeded JBRC's historical control data. Therefore, we conclude there is equivocal evidence for the carcinogenicity of aNTiO2 in rats. No lung tumors were observed in the satellite groups. Particle-induced non-neoplastic lesions (alveolar epithelial hyperplasia and focal fibrosis) were observed in exposed males and females in both the carcinogenicity and satellite groups. Increased lung weight and neutrophils of bronchoalveolar lavage fluid were observed in the 8 mg/m3-exposed carcinogenicity groups. The aNTiO2 deposited in the lungs of the satellite group rats was decreased at 26 weeks after the end of exposure compared to 1 day after the end of exposure. At 52 weeks after the end of exposure, the decreased level was the same at 26 weeks after the end of exposure.
Asunto(s)
Exposición por Inhalación , Neoplasias Pulmonares , Ratas Endogámicas F344 , Titanio , Animales , Titanio/toxicidad , Titanio/administración & dosificación , Masculino , Neoplasias Pulmonares/inducido químicamente , Neoplasias Pulmonares/patología , Femenino , Exposición por Inhalación/efectos adversos , Aerosoles , Pulmón/patología , Pulmón/efectos de los fármacos , Nanopartículas/toxicidad , RatasRESUMEN
BACKGROUND: After suffering from COVID-19, a large number of patients need respiratory rehabilitation. One of the methods of rehabilitation is inhalation with salt aerosols. OBJECTIVE: Our work aimed to study the effectiveness of inhalations of a dry aerosol of salt precipitated from the mineral water of the "Teplitsa multidisciplinary sanatorium", Transcarpathian region of Ukraine. MATERIAL AND METHODS: 30 male patients were examined after suffering from COVID-19. We formed two groups of patients, control and main, 15 people each. Patients in the control group received inhalation with a dry aerosol of table salt of the "Aero-M-sol". In contrast, patients in the main group received a course of inhalations with a dry aerosol of salt precipitated from the mineral water. RESULTS AND DISCUSSION: Under the influence of the rehabilitation complex in both groups, there is a performance improvement but significant changes are observed only in patients of the main group. The indicator Forced Vital Сapacity1 increased to the greatest extent, which after rehabilitation is significantly higher than in the control group (p<0.05). As a result, the Tiffeneau index significantly increases in the main group compared to the control group, reaching normal values. The main effect is associated with a decrease in obstructive complications of the respiratory tract as a result of a decrease in inflammation. The use of iodine-bromine brines (as in our case) for inhalation in the treatment of respiratory diseases has been proven to be effective, with systemic effects in the form of decreased IgE and increased IgA in the blood serum having been noticed. CONCLUSIONS: The use of haloinhalations with MW salts in the rehabilitation of patients after suffering from COVID-19 disease significantly improves the clinical condition of convalescents.
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COVID-19 , Colonias de Salud , Humanos , COVID-19/rehabilitación , Masculino , Ucrania , Persona de Mediana Edad , Administración por Inhalación , Adulto , SARS-CoV-2 , Aerosoles , Aguas MineralesRESUMEN
Radiocarbon measurements of total carbon (TC) fraction of aerosol samples collected at the campus of the Comenius University in Bratislava (Slovakia) during 2022-2023 were carried out. Based on radiocarbon activity of these samples and a source apportionment model we have determined the relative proportion of fossil and non-fossil carbon in collected atmospheric aerosols. The carbon from non-fossil sources (biomass burning and biogenic emissions) was dominant in this time period, on average it formed 72% of carbon present in the aerosols from the atmosphere of Bratislava. The whole range of determined non-fossil fraction was relatively small as it varied only from 0.67 (August-September) to 0.82 (December-January). These changes do not exhibit any significant seasonal variation as was previously observed in Bratislava during 2017-2018 in the elemental carbon (EC) aerosol fraction.
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Aerosoles , Contaminantes Atmosféricos , Carbono , Aerosoles/análisis , Carbono/análisis , Contaminantes Atmosféricos/análisis , Eslovaquia , Monitoreo del Ambiente/métodos , Atmósfera/química , FósilesRESUMEN
Secondhand vaping exposure is an emerging public health concern that remains understudied. In this study, saliva and exhaled emissions from ENDS users (secondhand) and non-ENDS users (baseline) were collected, firsthand emissions were generated using an automated ENDS aerosol generation system programmed to simulate puffing topography profiles collected from ENDS users. Particulate concentrations and sizes along with volatile organic compounds were characterized. We revealed puffing topography metrics as potential mediators of firsthand and secondhand particle and chemical exposures, as well as metabolic and respiratory health outcomes. Particle deposition modeling revealed that while secondhand emissions displayed smaller deposited mass, total and pulmonary particle deposition fractions were higher than firsthand deposition levels, possibly due to smaller secondhand emission particle diameters. Lastly, untargeted metabolomic profiling of salivary biomarkers of lung injury due to firsthand ENDS exposures revealed potential early indicators of respiratory distress that may also be relevant in bystanders exposed to secondhand vaping scenarios. By leveraging system toxicology, we identified 10 metabolites, including leukotriene D4, that could potentially serve as biomarkers for ENDS use, exposure estimation, and the prediction of vaping-related disease. This study highlights characterization of vaping behavior is an important exposure component in advancing our understanding of potential health effects in ENDS users and bystanders.
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Vapeo , Humanos , Vapeo/efectos adversos , Proyectos Piloto , Masculino , Adulto , Femenino , Biomarcadores , Exposición por Inhalación/efectos adversos , Exposición por Inhalación/análisis , Saliva/metabolismo , Compuestos Orgánicos Volátiles/análisis , Compuestos Orgánicos Volátiles/metabolismo , Sistemas Electrónicos de Liberación de Nicotina , Aerosoles/efectos adversos , Adulto JovenRESUMEN
Surgical removal of epidermoid cysts can result in "cyst spray," posing a nuisance and risk to the surgeon, staff, and patients. Employing simple techniques at the time of anesthesia administration may decrease exposure to cyst spray. We describe 2 methods that can help: (1) placing an antiseptic-soaked gauze pad over a premarked lesion, or (2) covering the cyst with a clear biohazard bag.
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Quiste Epidérmico , Humanos , Quiste Epidérmico/cirugía , Antiinfecciosos Locales/administración & dosificación , AerosolesRESUMEN
The photolysis of particulate nitrate (pNO3-) has been suggested to be an important source of nitrous acid (HONO) in the troposphere. However, determining the photolysis rate constant of pNO3- (jpNO3-) suffers from high uncertainty. Prior laboratory measurements of jpNO3- using aerosol filters have been complicated by the "shadow effect"âa phenomenon of light extinction within aerosol layers that potentially skews these measurements. We developed a method to correct the shadow effect on the photolysis rate constant of pNO3- for HONO production (jpNO3- â HONO) using aerosol filters with identical chemical compositions but different aerosol loadings. We applied the method to quantify jpNO3- â HONO over the North China Plain (NCP) during the winter haze period. After correcting for the shadow effect, the normalized average jpNO3- â HONO at 5 °C increased from 5.89 × 10-6 s-1 to 1.72 × 10-5 s-1. The jpNO3- â HONO decreased with increasing pH and nitrate proportions in PM2.5 and had no correlation with nitrate concentrations. A parametrization for jpNO3- â HONO was developed for model simulation of HONO production in NCP and similar environments.
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Contaminantes Atmosféricos , Atmósfera , Nitratos , Ácido Nitroso , Fotólisis , Nitratos/química , Atmósfera/química , Ácido Nitroso/química , Contaminantes Atmosféricos/química , AerosolesRESUMEN
Environmentally persistent free radicals (EPFRs) play an important role in aerosol effects on air quality and public health, but their atmospheric abundance and sources are poorly understood. We measured EPFRs contained in PM2.5 collected in Fairbanks, Alaska, in winter 2022. We find that EPFR concentrations were enhanced during surface-based inversion and correlate strongly with incomplete combustion markers, including carbon monoxide and elemental carbon (R2 > 0.75). EPFRs exhibit moderately good correlations with PAHs, biomass burning organic aerosols, and potassium (R2 > 0.4). We also observe strong correlations of EPFRs with hydrocarbon-like organic aerosols, Fe and Ti (R2 > 0.6), and single-particle mass spectrometry measurements reveal internal mixing of PAHs, with potassium and iron. These results suggest that residential wood burning and vehicle tailpipes are major sources of EPFRs and nontailpipe emissions, such as brake wear and road dust, may contribute to the stabilization of EPFRs. Exposure to the observed EPFR concentrations (18 ± 12 pmol m-3) would be equivalent to smoking â¼0.4-1 cigarette daily. Very strong correlations (R2 > 0.8) of EPFR with hydroxyl radical formation in surrogate lung fluid indicate that exposure to EPFRs may induce oxidative stress in the human respiratory tract.
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Contaminantes Atmosféricos , Emisiones de Vehículos , Madera , Madera/química , Alaska , Radicales Libres , Material Particulado , Monitoreo del Ambiente , Aerosoles , Hidrocarburos Policíclicos Aromáticos/análisisRESUMEN
Face-to-face meetings on a conference table are a frequent form of communication. The short-range exposure risk of aerosol disease transmission is high in the scenario of susceptible facing the infectious person over the table. We propose a mitigation methodology using the air curtain to reduce direct exposure to virus-laden aerosols. A numerical model was validated with experimental data to simulate the dispersion of aerosols. A dynamic mesh was adopted to consider the head movement of a 3D thermal manikin model. Results show that nodding head increase the potential risk by 74 % compared to motionless. Subsequently, for a single air curtain, placing it in the middle of the table is more effective in preventing risks than on the sides. For double air curtains, increasing the distance between them has a greater risk reduction effect than a shorter distance. Increasing the air velocity or width is more effective than increasing the number of air curtains. A moderate velocity (1 m s-1) works well to reduce the risk of nasal breathing. A higher velocity (2 m s-1) is needed for the coughing scenario. For similar indoor environments, the air curtains on the table can offer active precautions without changing the current ventilation system.
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Maniquíes , Humanos , Aerosoles y Gotitas Respiratorias , COVID-19/prevención & control , COVID-19/transmisión , Aerosoles , SARS-CoV-2 , Exposición por Inhalación/prevención & controlRESUMEN
This paper presents a numerical investigation to understand the transport and deposition of sprays emitted by an impinging-jet inhaler in the human respiratory tract under different inhalation flow rates. An injection model is used for the numerical simulations considering the spreading angles of the spray in the two directions, which are measured from experiments. The model parameter is adjusted to match the mean droplet size measured in the previous experiment. A time-varying sinusoidal inhalation flow rate is utilized as airflow conditions, which is closer to the actual situation when using an inhaler. The results demonstrate that the inhalation airflow rate significantly affects the spray's transport behavior and deposition results in the respiratory tract. Both excessively high and low inhalation flow rates lead to an increase in deposition in the mouth-throat. A moderate inhalation flow rate reduces throat deposition while maximizing lung deposition. Higher inhalation flow rates enable faster delivery of the droplets to the lungs, whereas lower inhalation flow rates achieve a more uniform deposition over time in the lungs. The amount of deposition in different parts of the lung lobes follows a fixed order. This study provides valuable insights for optimizing the inhalation flow rate conditions of the impinging-jet inhaler for clinical applications.
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Nebulizadores y Vaporizadores , Humanos , Administración por Inhalación , Sistema Respiratorio/metabolismo , Aerosoles , Pulmón/metabolismo , Tamaño de la Partícula , Diseño de Equipo , Modelos Biológicos , Simulación por ComputadorRESUMEN
Dry powder inhalers (DPIs) are the first choice for inhalation drug development. However, some conventional DPI formulation processes require heating, which may damage high molecular weight drugs such as proteins and nucleic acids. In this study, we propose a novel DPI preparation process that avoids the use of heat. Dry powders were prepared by cryomilling nanofiber mats composed of polyvinyl alcohol, D(-)-mannitol (Man), and α-chymotrypsin (α-Chy) as the model drug using the electrospinning method. The addition of Man conferred high dispersibility and excellent in vitro aerosol performance to the nanofiber mat powder in a very short milling time (less than 0.5 min) as assessed using the Andersen cascade impactor. Powders were classified according to the degree of friability, and among these, nanofiber mats containing 15 % Man and milled for 0.25 min exhibited the highest aerosol performance. Nanofiber mats containing Man milled for less than 0.5 min also exhibited greater α-Chy enzymatic activity than a nebulized α-Chy solution. Furthermore, single inhalation induced no significant lung tissue damage as evidenced by lactate dehydrogenase activity assays of mouse bronchoalveolar lavage fluid. This novel DPI formulation process may facilitate the safe and efficient inhalational delivery of therapeutic proteins.
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Aerosoles , Quimotripsina , Manitol , Nanofibras , Nanofibras/química , Nanofibras/administración & dosificación , Animales , Administración por Inhalación , Manitol/química , Quimotripsina/química , Ratones , Inhaladores de Polvo Seco , Alcohol Polivinílico/química , Polvos , Sistemas de Liberación de Medicamentos , Pulmón/metabolismo , Líquido del Lavado Bronquioalveolar/química , MasculinoRESUMEN
Lubricating base oils have been extensively employed for producing various industrial and consumer products. Therefore, their environmental and health impacts should be carefully evaluated. Although there have been many reports on pulmonary cytotoxicity and inflammatory responses of inhaled lubricating base oils, their potential influences on pulmonary surfactant (PS) films that play an essential role in maintaining respiratory mechanics and pulmonary immunity remains largely unknown. Here a systematic study on the interactions between an animal-derived natural PS and aerosols of water and representative mineral and vegetable base oils is performed using a novel biophysical assessing technique called constrained drop surfactometry capable of providing in vitro simulations of normal tidal breathing and physiologically relevant temperature and humidity in the lung. It was found that the mineral oil aerosols can impose strong inhibitions to the biophysical property of PS film, while the airborne vegetable oils and water show negligible adverse effects within the studied concentration range. The inhibitory effect is originated from the strong hydrophobicity of mineral oil, which makes it able to disrupt the interfacial molecular ordering of both phospholipid and protein compositions and consequently suppress the formation of condensed phase and multilayer scaffolds in a PS film. ENVIRONMENTAL IMPLICATION: Understanding the biophysical influence of airborne lubricating base oils on pulmonary surfactant (PS) films can provide new insights into the environmental impacts and health concerns of various industrial lubricant products. Here a comparative study on interactions between an animal-derived natural PS film and the aerosols of water and representative mineral and vegetable base oils under the true physiological conditions was conducted in situ using constrained drop surfactometry. We show that the most frequently used mineral base oil can cause strong inhibitions to the PS film by disrupting the molecular ordering of saturated phospholipids and surfactant-associated proteins at the interface.
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Aerosoles , Lubricantes , Surfactantes Pulmonares , Aerosoles/química , Surfactantes Pulmonares/química , Lubricantes/química , Aceite Mineral/química , Animales , Aceites de Plantas/química , Fosfolípidos/química , Agua/químicaRESUMEN
PM2.5 is a main atmospheric pollutant with various sources and complex chemical compositions, which are influenced by various factors, such as anthropogenic emissions (AE) and meteorological conditions (MC). MC have a significant impacts on variations in atmospheric pollutant; therefore, emission reduction policies and ambient air quality are non-linearly correlated, which hinders the accurate assessment of the effectiveness of control measures. In this study, we conducted online observations of PM2.5 and its chemical composition in Hohhot, China, from December 1, 2019, to February 29, 2020, to investigate how the chemical compositions of PM2.5 respond to the variations in AE and MC. Moreover, the random forest (RF) model was used to quantify the contributions of AE and MC to PM2.5 and its chemical composition during severe hazes and the COVID-19 pandemic lockdown period. During the clean period, MC reduced PM2.5 concentrations by 124%, while MC incresed PM2.5 concentrations by 49% during severe pollution episode. Inorganic aerosols (SO42-, NO3-, and NH4+) showed the strongest response to MC. MC significantly contributed to PM2.5 (36%), SO42- (32%), NO3- (29%), NH4+ (28%), OC (22%), and SOC (17%) levels during pollution episodes. From the pre-lockdown to lockdown period, AE (MC) contributed 52% (48%), 81% (19%), 48% (52%), 68% (32%), 59% (41%), and 288% (-188%) to the PM2.5, SO42-, NO3-, NH4+, OC, and SOC reductions, respectively. The variations in MC (especially the increase in relative humidity) rapidly generated meteorologically sensitive species (SO42-, NO3-, and NH4+), which led to severe winter pollution. This study provides a reference for assessing the net benefits of emission reduction measures for PM2.5 and its chemical compositions.