RESUMEN
Utilization of in vitro (cellular) techniques, like Cell Painting and transcriptomics, could provide powerful tools for agrochemical candidate sorting and selection in the discovery process. However, using these models generates challenges translating in vitro concentrations to the corresponding in vivo exposures. Physiologically based pharmacokinetic (PBPK) modeling provides a framework for quantitative in vitro to in vivo extrapolation (IVIVE). We tested whether in vivo (rat liver) transcriptomic and apical points of departure (PODs) could be accurately predicted from in vitro (rat hepatocyte or human HepaRG) transcriptomic PODs or HepaRG Cell Painting PODs using PBPK modeling. We compared two PBPK models, the ADMET predictor and the httk R package, and found httk to predict the in vivo PODs more accurately. Our findings suggest that a rat liver apical and transcriptomic POD can be estimated utilizing a combination of in vitro transcriptome-based PODs coupled with PBPK modeling for IVIVE. Thus, high content in vitro data can be translated with modest accuracy to in vivo models of ultimate regulatory importance to help select agrochemical analogs in early stage discovery program.
Asunto(s)
Agroquímicos , Animales , Ratas , Humanos , Agroquímicos/farmacocinética , Agroquímicos/toxicidad , Hepatocitos/metabolismo , Hígado/metabolismo , Modelos Biológicos , Masculino , Transcriptoma , Línea Celular , Medición de RiesgoRESUMEN
Pesticide residues in crop plants are routinely measured in an edible commodity or in feed items to determine safe use. Pesticides present as dislodgeable foliar residues (DFR) are measured for use in non-dietary risk assessments where worker, resident and bystander re-entry may lead to contact with the treated foliage. Possible correlations between dietary and DFR residue decline have been investigated considering data from 177 dietary residue trials along with 56 DFR trials from outdoor studies on the same crops besides residue decline data available in the Plant Properties Database (PPDB).The residue studies proved to follow the non-normal distribution and the comparison between DT50 of both types of residues for all the active substances revealed higher numerical DT50 mean values of the dietary residue compared to the DFRs. The dissipation from dietary residue studies is slower with a higher population mean compared to the mean for DFR studies for most active substances studied. A DT50 value from dietary residue studies could potentially act as a conservative surrogate DT50 for DFR which could be useful in determining the length of DFR studies and benefit both the agrochemical industry and the regulatory bodies in supporting non-dietary pesticide risk assessment.
Asunto(s)
Agroquímicos/análisis , Dieta , Exposición a Riesgos Ambientales/análisis , Residuos de Plaguicidas/análisis , Agroquímicos/farmacocinética , Semivida , Exposición Profesional/análisis , Residuos de Plaguicidas/farmacocinética , Medición de RiesgoRESUMEN
Following adoption of the new OECD test guideline (TG) 474 for the in vivo mammalian erythrocyte micronucleus (MN) test (29 July 2016), demonstration of exposure of target tissue (bone marrow) is required, if the test result is negative i.e. no cytogenetic damage. It implies that for many active ingredients, relevant metabolites or significant impurities with existing in vivo MN tests resulting in negative genotoxicity findings, evidence of target tissue exposure may be lacking and is considered a data gap in regulatory reviews. We present here toxicokinetic (TK) testing strategies for the design and conduct of studies that would demonstrate evidence of delivery of the test substance to the bone marrow. To illustrate this, three examples are presented with methods utilized under each scenario. We also propose a decision tree that may help design suitable TK studies to establish evidence of bone marrow exposure.
Asunto(s)
Agroquímicos/farmacocinética , Agroquímicos/toxicidad , Médula Ósea/efectos de los fármacos , Médula Ósea/metabolismo , Árboles de Decisión , Pruebas de Micronúcleos , Animales , Femenino , Masculino , Ratas Sprague-Dawley , ToxicocinéticaRESUMEN
The synthesis and characterisation of novel polyphosphazene nanocarriers, based on hydrophilic polyalkylene oxide Jeffamine M1000 and hydrophobic steroids with a glycinate linker for pH-controlled release of diosgenin and two brassinosteroids (DI31 and S7) with agrochemical and potential anticancer activity, is hereby described. Polyphosphazenes carrying approximately 17 wt% of DI31 or S7 self-assembled in water to form 120-150 nm nanoaggregates, which showed an excellent plant growth effect on radish cotyledons due to sustained delivery of approximately 30% of the agrochemicals after 4 days. Cytotoxic evaluation showed that all polymers carrying steroids and Jeffamine M1000 resulted in strong to moderate toxicity to MCF-7 cancer cells and were non-toxic to primary human lung fibroblast cells at 0.1 to 0.025 mg mL-1. Thus, DI31 and S7 bearing polymers applied at 10-4 to 10-6 mg mL-1 for delivery of recommended DI31 or S7 quantities to crops should be harmless to humans. Particularly, DI31 and S7 bearing polymers with strong cytotoxicity on MCF-7 and non-toxicity on primary human lung fibroblasts, good cell uptake after 6 hours, proper hydrodynamic sizes between 100 and 200 nm, and slow sustained release of cytotoxic drugs (DI31, S7) in acidic conditions might potentiate their accumulation in cancer tissues with good antitumour effects and minor side effects. These results demonstrated that preparation of brassinosteroid bearing polymers is a promising strategy for the preparation of better agrochemicals with reduced pollutant impact on sustainable agriculture and potential anticancer formulations based on analogues of brassinosteroids.
Asunto(s)
Agroquímicos/farmacocinética , Antineoplásicos/farmacocinética , Portadores de Fármacos/química , Nanopartículas/química , Compuestos Organofosforados/metabolismo , Polímeros/metabolismo , Brasinoesteroides/farmacocinética , Células Cultivadas , Diosgenina/farmacocinética , Liberación de Fármacos , Fibroblastos/efectos de los fármacos , Humanos , Células MCF-7 , Reguladores del Crecimiento de las Plantas/farmacocinéticaRESUMEN
We describe a novel experimental method that mimics exposure to dried agrochemical residues on contact surfaces during re-entry into crops. It includes the creation of dry dislodgeable residues and subsequent transfer to human skin for in vitro measurement of dermal absorption within a standard Organisation for Economic Co-operation and Development test guideline (OECD TG) 428 study. A pre-determined volume of spray containing 14C-labelled active substance is transferred onto a polytetrafluorethylene-coated septum and air-dried. The septum is then gently placed onto the pre-wetted skin mounted in a flow-through Franz diffusion chamber. The septum is gently rotated thrice to transfer the dose. Preliminary tests determined transfer efficiency to ensure the appropriate test concentration on the skin. Then, a standard dermal absorption study is performed according to OECD TG 428. Results from 10 compounds indicate that the methodology can be robustly incorporated into a standard TG study. These data show that the dermal absorption from a dry dislodgeable residue is lower than that from the equivalent dose of the aqueous spray, regardless of formulation type or active substance. Studies following the scenario described above can be a suitable tool to better estimate dermal absorption from dry residues in re-entry worker and resident exposure assessment for agrochemicals.
Asunto(s)
Agroquímicos/metabolismo , Organización para la Cooperación y el Desarrollo Económico , Residuos de Plaguicidas/metabolismo , Absorción Cutánea , Piel/metabolismo , Agroquímicos/química , Agroquímicos/farmacocinética , Difusión , Humanos , Residuos de Plaguicidas/química , Residuos de Plaguicidas/farmacocinética , Piel/químicaRESUMEN
This study explored the characteristics of a newly isolated glyphosate (GLYP)-degrading bacterium Providencia rettgeri GDB 1, for GLYP bioremediation. Due to the serial selection pressure of high GLYP concentrations for enriched isolation, this highly tolerant GLYP biodegrader shows very promising capabilities for GLYP removal (approximately 71.4% degradation efficiency) compared to previously reported strains. High performance liquid chromatography analyses showed aminomethylphosphonic acid (AMPA) rather than sarcosine (SAR) to be the sole intermediate of GLYP decomposition via the AMPA formation pathway. Moreover, GLYP biodegradation was biochemically favorable in aerobic cultures due to its strong growth-associated characteristics. To the best of our knowledge, this is the first report to indicate that bacterial strains in the Providencia genus could demonstrate highly promising GLYP-degrading characteristics in environments with high GLYP contents.
Asunto(s)
Glicina/análogos & derivados , Herbicidas/farmacocinética , Providencia/metabolismo , Contaminantes Químicos del Agua/farmacocinética , Adaptación Biológica , Agricultura/métodos , Agroquímicos/aislamiento & purificación , Agroquímicos/farmacocinética , Agroquímicos/toxicidad , Biodegradación Ambiental , Cromatografía Líquida de Alta Presión/métodos , Glicina/aislamiento & purificación , Glicina/farmacocinética , Glicina/toxicidad , Herbicidas/aislamiento & purificación , Herbicidas/toxicidad , Humanos , Isoxazoles/química , Isoxazoles/metabolismo , Redes y Vías Metabólicas , Providencia/efectos de los fármacos , Providencia/enzimología , Providencia/crecimiento & desarrollo , Sarcosina/química , Sarcosina/metabolismo , Tetrazoles/química , Tetrazoles/metabolismo , Contaminantes Químicos del Agua/aislamiento & purificación , Contaminantes Químicos del Agua/toxicidad , Purificación del Agua/métodos , GlifosatoRESUMEN
Systemicity of agrochemicals is an advantageous property for controlling phloem sucking insects, as well as pathogens and pests not accessible to contact products. After the penetration of the cuticle, the plasma membrane constitutes the main barrier to the entry of an agrochemical into the sap flow. The current strategy for developing systemic agrochemicals is to optimize the physicochemical properties of the molecules so that they can cross the plasma membrane by simple diffusion or ion trapping mechanisms. The main problem with current systemic compounds is that they move everywhere within the plant, and this non-controlled mobility results in the contamination of the plant parts consumed by vertebrates and pollinators. To achieve the site-targeted distribution of agrochemicals, a carrier-mediated propesticide strategy is proposed in this review. After conjugating a non-systemic agrochemical with a nutrient (α-amino acids or sugars), the resulting conjugate may be actively transported across the plasma membrane by nutrient-specific carriers. By applying this strategy, non-systemic active ingredients are expected to be delivered into the target organs of young plants, thus avoiding or minimizing subsequent undesirable redistribution. The development of this innovative strategy presents many challenges, but opens up a wide range of exciting possibilities. © 2018 Society of Chemical Industry.
Asunto(s)
Agroquímicos/química , Agroquímicos/metabolismo , Portadores de Fármacos/química , Agroquímicos/farmacocinética , Animales , Disponibilidad Biológica , Transporte Biológico , Portadores de Fármacos/metabolismo , Plantas/metabolismo , Profármacos/metabolismoRESUMEN
In vitro methods have gained regulatory acceptance for the prediction of serious eye damage (UN GHS Cat 1). However, the majority of in vitro methods do not state whether they are applicable to agrochemical formulations. This manuscript presents a study of up to 27 agrochemical formulations tested in three in vitro assays (three versions of the bovine corneal opacity and permeability test (BCOP, OECD TG 437) assay, the isolated chicken eye test (ICE, OECD TG 438) and the EpiOcular™ ET-50 assay). The results were compared with already-available in vivo data. In the BCOP only one of the four, one of five in the ICE and six of eleven tested formulations in the EpiOcular™ ET-50 Neat Protocol resulted in the correct UN GHS Cat 1 prediction. Overpredictions occurred in all assays. These data indicate a lack of applicability of the three in vitro methods to reliably predict UN GHS Cat 1 of agrochemical formulations. In order to ensure animal-free identification of seriously eye damaging agrochemical formulations testing protocols and/or prediction models need to be modified or classification rules should be tailored to in vitro testing rather than using in vivo Draize data as a standard.
Asunto(s)
Agroquímicos/clasificación , Agroquímicos/toxicidad , Ojo/efectos de los fármacos , Irritantes/clasificación , Irritantes/toxicidad , Agroquímicos/farmacocinética , Alternativas a las Pruebas en Animales , Animales , Bovinos , Pollos , Opacidad de la Córnea , Ojo/metabolismo , Femenino , Humanos , Técnicas In Vitro , Irritantes/farmacocinética , Masculino , Permeabilidad , Conejos , Pruebas de ToxicidadRESUMEN
A strategic and comprehensive program in which toxicokinetic (TK) measurements are made for all agrochemicals undergoing toxicity testing (both new compounds and compounds already registered for use) is described. This approach provides the data to more accurately assess the toxicokinetics of agrochemicals and their metabolites in laboratory animals and humans. Having this knowledge provides the ability to conduct more insightful toxicity studies, refine and interpret exposure assessments and reduce uncertainty in risk assessments. By developing a better understanding of TK across species, including humans via in vitro metabolism studies, any differences across species in TK can be identified early and the most relevant species can be selected for toxicity tests. It also provides the ability to identify any non-linearities in TK as a function of dose, which in turn can be used to identify a kinetically derived maximum dose (KMD) and avoid dosing inappropriately outside of the kinetic linear range. Measuring TK in key life stages also helps to identify changes in ADME parameters from in utero to adults. A robust TK database can also be used to set internal concentration based "Reference Concentrations" and Biomonitoring Equivalents (BE), and support selection of Chemical Specific Adjustment Factors (CSAF). All of these factors support the reduction of uncertainty throughout the entire risk assessment process. This paper outlines how a TK research strategy can be integrated into new agrochemical toxicity testing programs, together with a proposed Framework for future use.
Asunto(s)
Agroquímicos/farmacocinética , Agroquímicos/toxicidad , Medición de Riesgo/métodos , Animales , Relación Dosis-Respuesta a Droga , Exposición a Riesgos Ambientales/análisis , Humanos , ToxicocinéticaRESUMEN
Copper sulfates (CuSO4) are widely used as the primary component of fungicides in the grape industry. The agricultural-grade CuSO4 that we collected from Chinese nationwide markets were found to be contaminated by polychlorinated dibenzo-p-dioxins and dibenzofurans and high levels of polychlorinated biphenyls (Σ19PCBs: 0.32~9.51 ng/g). In the following research, we studied the impact of CuSO4 application on PCB levels in grape products through a field experiment, and conducted a national survey to speculate the role that CuSO4 played on the occurrence of PCB in grapes. In the field experiment, an obvious increase of PCBs in grape leaves (from 174 to 250 pg/g fw) was observed after Bordeaux mixture (the main component of which is CuSO4) application. As to the main PCB congener in CuSO4, the most toxic CB 126 (toxic equivalency factor = 0.1) also increased in grape peels (from 1.66 to 2.93 pg/g fw) after pesticide spray. Both the correlation study and the principal component analysis indicated that environmental factors were dominant PCB contributors to grapes, and grapes from e-waste dismantling area containing the highest PCBs also proved the notion. It is worth noting that this report describes the first research examining PCBs in CuSO4 and its influence on agricultural products to date.
Asunto(s)
Agroquímicos/análisis , Bifenilos Policlorados/análisis , Vitis/química , Agroquímicos/farmacocinética , Agroquímicos/toxicidad , China , Sulfato de Cobre/química , Sulfato de Cobre/farmacocinética , Bifenilos Policlorados/farmacocinética , Bifenilos Policlorados/toxicidad , Vitis/efectos de los fármacos , Vitis/metabolismoRESUMEN
Dermal absorption is a critical part in the risk assessment of complex mixtures such as agrochemical formulations. To reduce the number of in vivo or in vitro absorption experiments, the present study aimed to develop an in silico prediction model that considers mixture-related effects. Therefore, an experimental 'real-world' dataset derived from regulatory in vitro studies with human and rat skin was processed. Overall, 56 test substances applied in more than 150 mixtures were used. Descriptors for the substances as well as the mixtures were generated and used for multiple linear regression analysis. Considering the heterogeneity of the underlying data set, the final model provides a good fit (r² = 0.75) and is able to estimate the influence of a newly composed formulation on dermal absorption of a well-known substance (predictivity Q²Ext = 0.73). Application of this model would reduce animal and non-animal testings when used for the optimization of formulations in early developmental stages, or would simplify the registration process, if accepted for read-across.
Asunto(s)
Agroquímicos/farmacocinética , Simulación por Computador , Relación Estructura-Actividad Cuantitativa , Absorción Cutánea , Piel/metabolismo , Animales , Mezclas Complejas/farmacocinética , Humanos , Modelos Lineales , Modelos Biológicos , RatasRESUMEN
The identification of the no observed adverse effect level (NOAEL) is the key regulatory outcome of toxicity studies. With the introduction of "omics" technologies into toxicological research, the question arises as to how sensitive these technologies are relative to classical regulatory toxicity parameters. BASF SE and metanomics developed the in vivo metabolome database MetaMap®Tox containing metabolome data for more than 500 reference compounds. For several years metabolome analysis has been routinely performed in regulatory toxicity studies (REACH mandated testing or new compound development), mostly in the context of 28 day studies in rats (OECD 407 guideline). For those chemicals for which a toxicological NOAEL level was obtained at either high or mid-dose level, we evaluated the associated metabolome to investigate the sensitivity of metabolomics versus classical toxicology with respect to the NOAEL. For the definition of a metabolomics NOAEL the ECETOC criteria (ECETOC, 2007) were used. In this context we evaluated 104 cases. Comparable sensitivity was noted in 75% of the cases, increased sensitivity of metabolomics in 8%, and decreased sensitivity in 18% of the cases. In conclusion, these data suggest that metabolomics profiling has a similar sensitivity to the classical toxicological study (e.g. OECD 407) design.
Asunto(s)
Agroquímicos/toxicidad , Evaluación Preclínica de Medicamentos , Drogas en Investigación/efectos adversos , Metabolómica/métodos , Modelos Biológicos , Pruebas de Toxicidad , Agroquímicos/análisis , Agroquímicos/farmacocinética , Animales , Bases de Datos de Compuestos Químicos , Evaluación Preclínica de Medicamentos/normas , Drogas en Investigación/análisis , Drogas en Investigación/farmacocinética , Femenino , Alemania , Guías como Asunto , Humanos , Legislación de Medicamentos , Masculino , Nivel sin Efectos Adversos Observados , Medicamentos sin Prescripción/efectos adversos , Medicamentos sin Prescripción/análisis , Medicamentos sin Prescripción/farmacocinética , Medicamentos bajo Prescripción/efectos adversos , Medicamentos bajo Prescripción/análisis , Medicamentos bajo Prescripción/farmacocinética , Ratas , Ratas Wistar , Reproducibilidad de los Resultados , Caracteres Sexuales , Pruebas de Toxicidad/normas , Toxicología/legislación & jurisprudencia , Toxicología/métodosRESUMEN
We investigated the suitability of an active biomonitoring approach, using the ecologically relevant species Gammarus fossarum, to assess trends of bioavailable contamination in continental waters. Gammarids were translocated into cages at 27 sites, in the Rhône-Alpes region (France) during early autumn 2009. Study sites were chosen to represent different physico-chemical characteristics and various anthropic pressures. Biotic factors such as sex, weight and food availability were controlled in order to provide robust and comparable results. After one week of exposure, concentrations of 11 metals/metalloids (Cd, Pb, Hg, Ni, Zn, Cr, Co, Cu, As, Se and Ag) and 38 hydrophobic organic substances including polycyclic aromatic hydrocarbons (PAHs), polychlorobiphenyles (PCBs), pentabromodiphenylethers (PBDEs) and organochlorine pesticides, were measured in gammarids. All metals except Ag, and 33 organic substances among 38 were quantified in G. fossarum, showing that this species is relevant for chemical biomonitoring. The control of biotic factors allowed a robust and direct inter-site comparison of the bioavailable contamination levels. Overall, our results show the interest and robustness of the proposed methodological approach for assessing trends of bioavailable contamination, notably for metals and hydrophobic organic contaminants, in continental waters. Furthermore, we built threshold values of bioavailable contamination in gammarids, above which measured concentrations are expected to reveal a bioavailable contamination at the sampling site. Two ways to define such values were investigated, a statistical approach and a model fit. Threshold values were determined for almost all the substances investigated in this study and similar values were generally derived from the two approaches. Then, levels of contaminants measured in G. fossarum at the 27 study sites were compared to the threshold values obtained using the model fit. These threshold values could serve as a basis for further implementation of quality grids to rank sites according to the extent of the bioavailable contamination, with regard to the applied methodology.
Asunto(s)
Agroquímicos/farmacocinética , Anfípodos/efectos de los fármacos , Monitoreo del Ambiente/métodos , Agua Dulce/química , Metales Pesados/farmacocinética , Modelos Biológicos , Contaminantes Químicos del Agua/farmacocinética , Agroquímicos/química , Agroquímicos/toxicidad , Anfípodos/química , Anfípodos/crecimiento & desarrollo , Anfípodos/metabolismo , Animales , Arsénico/química , Arsénico/farmacocinética , Arsénico/toxicidad , Disponibilidad Biológica , Fenómenos Químicos , Francia , Éteres Difenilos Halogenados/química , Éteres Difenilos Halogenados/farmacocinética , Éteres Difenilos Halogenados/toxicidad , Hidrocarburos Clorados/química , Hidrocarburos Clorados/farmacocinética , Hidrocarburos Clorados/toxicidad , Masculino , Metales Pesados/química , Metales Pesados/toxicidad , Plaguicidas/química , Plaguicidas/farmacocinética , Plaguicidas/toxicidad , Bifenilos Policlorados/química , Bifenilos Policlorados/farmacocinética , Bifenilos Policlorados/toxicidad , Hidrocarburos Policíclicos Aromáticos/química , Hidrocarburos Policíclicos Aromáticos/farmacocinética , Hidrocarburos Policíclicos Aromáticos/toxicidad , Ríos , Selenio/química , Selenio/farmacocinética , Selenio/toxicidad , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/toxicidadRESUMEN
Integrated toxicokinetics (TK) data provide information on the rate, extent and duration of systemic exposure across doses, species, strains, gender, and life stages within a toxicology program. While routine for pharmaceuticals, TK assessments of non-pharmaceuticals are still relatively rare, and have never before been included in a full range of guideline studies for a new agrochemical. In order to better understand the relationship between diurnal systemic dose (AUC(24h)) and toxicity of agrochemicals, TK analyses in the study animals is now included in all short- (excluding acute), medium- and long-term guideline mammalian toxicity studies including reproduction/developmental tests. This paper describes a detailed procedure for the implementation of TK in short-, medium- and long-term regulatory toxicity studies, without the use of satellite animals, conducted on three agrochemicals (X11422208, 2,4-D and X574175). In these studies, kinetically-derived maximum doses (KMD) from short-term studies instead of, or along with, maximum tolerated doses (MTD) were used for the selection of the high dose in subsequent longer-term studies. In addition to leveraging TK data to guide dose level selection, the integrated program was also used to select the most appropriate method of oral administration (i.e., gavage versus dietary) of test materials for rat and rabbit developmental toxicity studies. The integrated TK data obtained across toxicity studies (without the use of additional/satellite animals) provided data critical to understanding differences in response across doses, species, strains, sexes, and life stages. Such data should also be useful in mode of action studies and to improve human risk assessments.
Asunto(s)
Agroquímicos , Alternativas al Uso de Animales/métodos , Pruebas de Toxicidad/métodos , Agroquímicos/administración & dosificación , Agroquímicos/farmacocinética , Agroquímicos/toxicidad , Alternativas al Uso de Animales/estadística & datos numéricos , Animales , Simulación por Computador , Interpretación Estadística de Datos , Relación Dosis-Respuesta a Droga , Esquema de Medicación , Guías como Asunto , Humanos , Dosis Máxima Tolerada , Valor Predictivo de las Pruebas , Conejos , Ratas , Ratas Endogámicas F344 , Reproducción/efectos de los fármacos , Pruebas de Toxicidad/estadística & datos numéricosRESUMEN
The adsorption of nanostructured lyotropic liquid-crystal particles, cubosomes and hexosomes, at surfaces was investigated for potential use in surface-specific agrochemical delivery. Adsorption of phytantriol (PHYT) and glyceryl monooleate (GMO)-based cubosomes and hexosomes, stabilized using Pluronic F127, at tristearin-coated (model leaf surface) and uncoated zinc selenide surfaces was studied using attenuated total reflectance Fourier transform IR (ATR-FTIR) spectroscopy, by quantifying the IR absorbance due to the lipid components of the particles over time. The delivery of an encapsulated hydrophobic model herbicide [dichlorodiphenyldichloroethylene (DDE)] was also examined on the model and real leaf surfaces. The adsorption behavior of the particles by ATR-FTIR was dependent on the internal nanostructure and lipid composition, with PHYT cubosomes adsorbing more avidly at tristearin surfaces than GMO-based cubosomes or hexosomes. There was a direct correlation between DDE associated with the surfaces and the particle adsorption observed in the ATR-FTIR study, strongly implicating particle adsorption with the delivery efficiency. Differences between the mode of interaction of the Pluronic stabilizer with the different lipids and particle nanostructures were proposed to lead to differences in the particle adsorption behavior.
Asunto(s)
Agroquímicos/farmacocinética , Sistemas de Liberación de Medicamentos/métodos , Cristales Líquidos/química , Nanopartículas/química , Nanotecnología/métodos , Adsorción , Alcoholes Grasos/farmacocinética , Glicéridos/farmacocinéticaRESUMEN
The efficiency of vetiver grass (Vetiveria zizanioides) in removing 2,4,6-trinitrotoluene (TNT) from aqueous media was explored in the presence of a common agrochemical, urea, used as a chaotropic agent. Chaotropic agents disrupt water structure, increasing solubilization of hydrophobic compounds (TNT), thus, enhancing plant TNT uptake. The primary objectives of this study were to: (i) characterize TNT absorption by vetiver in hydroponic media, and (ii) determine the effect of urea on chemically catalyzing TNT uptake by vetiver grass in hydroponic media. Results showed that vetiver exhibited a high TNT uptake capacity (1.026 mgg(-1)), but kinetics were slow. Uptake was considerably enhanced in the presence of urea, which significantly (p<0.001) increased the 2nd-order reaction rate constant over that of the untreated (no urea) control. Three major TNT metabolites were detected in the roots, but not in the shoot, namely 1,3,5-trinitrobenzene, 4-amino 2,6-dinitrotoluene, and 2-amino 4,6-dinitrotoluene, indicating TNT degradation by vetiver grass.
Asunto(s)
Agroquímicos/farmacocinética , Chrysopogon/metabolismo , Raíces de Plantas/metabolismo , Contaminantes del Suelo/farmacocinética , Trinitrotolueno/farmacocinética , Urea/metabolismo , Absorción , Agroquímicos/análisis , Biodegradación Ambiental , Catálisis , Hidroponía , Contaminantes del Suelo/análisis , Factores de Tiempo , Trinitrotolueno/análisisRESUMEN
A proposal has been developed by the Agricultural Chemical Safety Assessment (ACSA) Technical Committee of the ILSI Health and Environmental Sciences Institute (HESI) for an improved approach to assessing the safety of crop protection chemicals. The goal is to ensure that studies are scientifically appropriate and necessary without being redundant, and that tests emphasize toxicological endpoints and exposure durations that are relevant for risk assessment. Incorporation of pharmacokinetic studies describing absorption, distribution, metabolism, and excretion is an essential tool for improving the design and interpretation of toxicity studies and their application for safety assessment. A tiered approach is described in which basic pharmacokinetic studies, similar to those for pharmaceuticals, are conducted for regulatory submission. Subsequent tiers provide additional information in an iterative manner, depending on pharmacokinetic properties, toxicity study results, and the intended uses of the compound.
Asunto(s)
Agroquímicos/farmacocinética , Administración de la Seguridad , Agroquímicos/toxicidad , Animales , Humanos , Medición de RiesgoRESUMEN
The recent progress and future prospects for the successful application of combinatorial chemistry and high throughput screening within the agrochemical lead discovery process are outlined and discussed. Solid and solution phase library synthesis technologies are reviewed and compared, and the role and importance of bioavailability, diversity and virtual screening in rational library design are detailed.
Asunto(s)
Agroquímicos/química , Técnicas Químicas Combinatorias , Agroquímicos/farmacocinética , Química Agrícola/métodos , Solubilidad , Relación Estructura-ActividadRESUMEN
The demand for new herbicides, insecticides and fungicides led to a steady increase in the number of compounds being tested to find novel market products. To keep pace with the rising workload, high throughput screening (HTS) technologies have been introduced. In agrochemical research miniaturised in vivo tests on whole real target organisms are now possible and are an integral part of the screening cascade. A complementary target based in vitro HTS has also been established in agrochemical research. Target based HTS allows a directed approach towards untouched market shares by novel modes of action. Selection of the best suited targets is the most crucial issue in this approach. Genomic methods thereby deliver many essential genes as candidate targets. Consideration of further criteria such as druggability notably narrows down the number of promising targets. Though target to hit to lead progression still is as in pharmaceutical research a complex and therefore risky process, the implementation of novel bioscience technologies has entailed the transition to an integrated innovative agrochemical research perspective.
Asunto(s)
Agroquímicos/química , Técnicas Químicas Combinatorias , Agroquímicos/farmacocinética , Química Agrícola/métodos , Genoma de Planta/efectos de los fármacos , Relación Estructura-ActividadRESUMEN
Dermal contact with potentially toxic agricultural and industrial chemicals is a common hazard encountered in occupational, accidental spill and environmental contamination scenarios. Different solvents and chemical mixtures may influence dermal absorption. The effects of sodium lauryl sulphate (SLS) on the stratum corneum partitioning and permeability in porcine skin of 10 agricultural and industrial chemicals in water, ethanol and propylene glycol were investigated. The chemicals were phenol, p-nitrophenol, pentachlorophenol, methyl parathion, ethyl parathion, chlorpyrifos, fenthion, simazine, atrazine and propazine. SLS decreased partitioning into stratum corneum from water for lipophilic compounds, decreased partitioning from propylene glycol and did not alter partitioning from ethanol. SLS effects on permeability were less consistent, but generally decreased permeability from water, increased permeability from ethanol and had an inconsistent effect on permeability from propylene glycol. It was concluded that, for the compounds tested, partitioning into the stratum corneum was determined by the relative solubility of the solute in the donor solvent and the stratum corneum lipids. Permeability, however, reflected the result of successive, complex processes and was not predictable from stratum corneum partitioning alone. Addition of SLS to solvents altered partitioning and absorption characteristics across a range of compounds, which indicates that partition coefficients or skin permeability from neat chemical exposure should be used with caution in risk assessment procedures for chemical mixtures.