RESUMEN
BCS1L encodes a homolog of the Saccharomyces cerevisiae bcs1 protein, which has a known role in the assembly of Complex III of the mitochondrial respiratory chain. Phenotypes reported in association with pathogenic BCS1L variants include growth retardation, aminoaciduria, cholestasis, iron overload, lactic acidosis and early death (GRACILE syndrome), and Björnstad syndrome, characterized by abnormal flattening and twisting of hair shafts (pili torti) and hearing problems. Here we describe two patients harbouring biallelic variants in BCS1L; the first with a heterozygous variant c.166C>T, p.(Arg56*) together with a novel heterozygous variant c.205C>T, p.(Arg69Cys) and a second patient with a novel homozygous c.325C>T, p.(Arg109Trp) variant. The two patients presented with different phenotypes; the first patient presented as an adult with aminoaciduria, seizures, bilateral sensorineural deafness and learning difficulties. The second patient was an infant who presented with a classical GRACILE syndrome leading to death at 4 months of age. A decrease in BCS1L protein levels was seen in both patients, and biochemical analysis of Complex III revealed normal respiratory chain enzyme activities in the muscle of both patients. A decrease in Complex III assembly was detected in the adult patient's muscle, whilst the paediatric patient displayed a combined mitochondrial respiratory chain defect in cultured fibroblasts. Yeast complementation studies indicate that the two missense variants, c.205C>T, p.(Arg69Cys) and c.325C>T, p.(Arg109Trp), impair the respiratory capacity of the cell. Together, these data support the pathogenicity of the novel BCS1L variants identified in our patients.
Asunto(s)
ATPasas Asociadas con Actividades Celulares Diversas/genética , Complejo III de Transporte de Electrones/genética , Enfermedades Mitocondriales/genética , ATPasas Asociadas con Actividades Celulares Diversas/metabolismo , Acidosis Láctica/genética , Adulto , Secuencia de Aminoácidos , Colestasis/genética , Complejo III de Transporte de Electrones/metabolismo , Femenino , Retardo del Crecimiento Fetal/genética , Fibroblastos/metabolismo , Hemosiderosis/genética , Humanos , Lactante , Masculino , Errores Innatos del Metabolismo/genética , Enfermedades Mitocondriales/congénito , Músculo Esquelético/citología , Músculo Esquelético/metabolismo , Mutación , Fenotipo , Aminoacidurias Renales/genéticaRESUMEN
Lysinuric protein intolerance (LPI) is a recessively inherited aminoaciduria caused by mutations of SLC7A7, the gene encoding y+LAT1 light chain of system y+L for cationic amino acid transport. The pathogenesis of LPI is still unknown. In this study, we have utilized a gene silencing approach in macrophages and airway epithelial cells to investigate whether complications affecting lung and immune system are directly ascribable to the lack of SLC7A7 or, rather, mediated by an abnormal accumulation of arginine in mutated cells. When SLC7A7/y+LAT1 was silenced in human THP-1 macrophages and A549 airway epithelial cells by means of short interference RNA (siRNA), a significant induction of the expression and release of the inflammatory mediators IL1ß and TNFα was observed, no matter the intracellular arginine availability. This effect was mainly regulated at transcriptional level through the activation of NFκB signaling pathway. Moreover, since respiratory epithelial cells are the important sources of chemokines in response to pro-inflammatory stimuli, the effect of IL1ß has been addressed on SLC7A7 silenced A549 cells. Results obtained indicated that the downregulation of SLC7A7/y+LAT1 markedly strengthened the stimulatory effect of the cytokine on CCL5/RANTES expression and release without affecting the levels of CXCL8/IL8. Consistently, also the conditioned medium of silenced THP-1 macrophages activated airway epithelial cells in terms of CCL5/RANTES expression due to the presence of elevated amount of proinflammatory cytokines. In conclusion, our results point to a novel thus far unknown function of SLC7A7/y+LAT1, that, under physiological conditions, besides transporting arginine, may act as a brake to restrain inflammation.
Asunto(s)
Errores Innatos del Metabolismo de los Aminoácidos/inmunología , Cadenas Ligeras de la Proteína-1 Reguladora de Fusión/metabolismo , Inflamación/inmunología , Macrófagos/inmunología , Aminoacidurias Renales/inmunología , Mucosa Respiratoria/inmunología , Células A549 , Errores Innatos del Metabolismo de los Aminoácidos/genética , Sistema de Transporte de Aminoácidos y+L , Quimiocina CCL5/metabolismo , Cadenas Ligeras de la Proteína-1 Reguladora de Fusión/genética , Silenciador del Gen , Humanos , Inflamación/genética , Interleucina-1beta/metabolismo , Mutación/genética , FN-kappa B/metabolismo , Fenotipo , ARN Interferente Pequeño/genética , Aminoacidurias Renales/genética , Células THP-1 , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
BACKGROUND: Mitochondrial diseases due to defective respiratory chain complex III (CIII) are relatively uncommon. The assembly of the eleven-subunit CIII is completed by the insertion of the Rieske iron-sulfur protein, a process for which BCS1L protein is indispensable. Mutations in the BCS1L gene constitute the most common diagnosed cause of CIII deficiency, and the phenotypic spectrum arising from mutations in this gene is wide. RESULTS: A case of CIII deficiency was investigated in depth to assess respiratory chain function and assembly, and brain, skeletal muscle and liver histology. Exome sequencing was performed to search for the causative mutation(s). The patient's platelets and muscle mitochondria showed respiration defects and defective assembly of CIII was detected in fibroblast mitochondria. The patient was compound heterozygous for two novel mutations in BCS1L, c.306A > T and c.399delA. In the cerebral cortex a specific pattern of astrogliosis and widespread loss of microglia was observed. Further analysis showed loss of Kupffer cells in the liver. These changes were not found in infants suffering from GRACILE syndrome, the most severe BCS1L-related disorder causing early postnatal mortality, but were partially corroborated in a knock-in mouse model of BCS1L deficiency. CONCLUSIONS: We describe two novel compound heterozygous mutations in BCS1L causing CIII deficiency. The pathogenicity of one of the mutations was unexpected and points to the importance of combining next generation sequencing with a biochemical approach when investigating these patients. We further show novel manifestations in brain, skeletal muscle and liver, including abnormality in specialized resident macrophages (microglia and Kupffer cells). These novel phenotypes forward our understanding of CIII deficiencies caused by BCS1L mutations.
Asunto(s)
Acidosis Láctica/genética , Colestasis/genética , Retardo del Crecimiento Fetal/genética , Hemosiderosis/genética , Errores Innatos del Metabolismo/genética , Enfermedades Mitocondriales/congénito , Aminoacidurias Renales/genética , Animales , Transporte de Electrón/fisiología , Complejo III de Transporte de Electrones/genética , Flavoproteínas Transportadoras de Electrones/genética , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Ratones , Enfermedades Mitocondriales/genética , Encefalomiopatías Mitocondriales/genética , Mutación/genéticaRESUMEN
UNLABELLED: We report a novel homozygous missense mutation in the ubiquinol-cytochrome c reductase synthesis-like (BCS1L) gene in two consanguineous Turkish families associated with deafness, Fanconi syndrome (tubulopathy), microcephaly, mental and growth retardation. All three patients presented with transitory metabolic acidosis in the neonatal period and development of persistent renal de Toni-Debré-Fanconi-type tubulopathy, with subsequent rachitis, short stature, microcephaly, sensorineural hearing impairment, mild mental retardation and liver dysfunction. The novel missense mutation c.142A>G (p.M48V) in BCS1L is located at a highly conserved region associated with sorting to the mitochondria. Biochemical analysis revealed an isolated complex III deficiency in skeletal muscle not detected in fibroblasts. Native polyacrylamide gel electrophoresis (PAGE) revealed normal super complex formation, but a shift in mobility of complex III most likely caused by the absence of the BCS1L-mediated insertion of Rieske Fe/S protein into complex III. These findings expand the phenotypic spectrum of BCS1L mutations, highlight the importance of biochemical analysis of different primary affected tissue and underline that neonatal lactic acidosis with multi-organ involvement may resolve after the newborn period with a relatively spared neurological outcome and survival into adulthood. CONCLUSION: Mutation screening for BCS1L should be considered in the differential diagnosis of severe (proximal) tubulopathy in the newborn period. WHAT IS KNOWN: ⢠Mutations in BCS1L cause mitochondrial complex III deficiencies. ⢠Phenotypic presentations of defective BCS1L range from Bjornstad to neonatal GRACILE syndrome. What is New: ⢠Description of a novel homozygous mutation in BCS1L with transient neonatal acidosis and persistent de Toni-Debré-Fanconi-type tubulopathy. ⢠The long survival of patients with phenotypic presentation of severe complex III deficiency is uncommon.
Asunto(s)
Acidosis Láctica/genética , Colestasis/genética , Sordera/genética , Complejo III de Transporte de Electrones/deficiencia , Síndrome de Fanconi/genética , Retardo del Crecimiento Fetal/genética , Hemosiderosis/genética , Errores Innatos del Metabolismo/genética , Microcefalia/genética , Enfermedades Mitocondriales/congénito , Aminoacidurias Renales/genética , ATPasas Asociadas con Actividades Celulares Diversas , Adolescente , Adulto , Western Blotting , Diagnóstico Diferencial , Complejo III de Transporte de Electrones/genética , Electroforesis en Gel de Poliacrilamida , Síndrome de Fanconi/etiología , Femenino , Trastornos del Crecimiento/genética , Homocigoto , Humanos , Recién Nacido , Discapacidad Intelectual/genética , Masculino , Enfermedades Mitocondriales/genética , Mutación MissenseRESUMEN
Severe vitamin D deficiency (reduction in serum 25(OH)D concentration) in infants and children can cause features of the Fanconi syndrome, including phosphaturia, glycosuria, aminoaciduria, and renal tubular acidosis. This indicates that vitamin D and its metabolites influence proximal tubule function. Filtered 25(OH)D bound to vitamin D binding protein (DBP) is endocytosed by megalin-cubilin in the apical membrane. Intracellular 25(OH)D is metabolized to 1,25(OH)2D or calcitroic acid by 1-α-hydroxylase or 24-hydroxylase in tubule cell mitochondria. Bone-produced fibroblast growth factor 23 (FGF23) bound to Klotho in tubule cells and intracellular phosphate concentrations are regulators of 1-α-hydroxylase activity and cause proximal tubule phosphaturia. Aminoaciduria occurs when amino acid transporter synthesis is deficient, and 1,25(OH)2D along with retinoic acid up-regulate transporter synthesis by a vitamin D response element in the promoter region of the transporter gene. This review discusses evidence gained from studies in animals or cell lines, as well as from human disorders, that provide insight into vitamin D-proximal tubule interactions.
Asunto(s)
Túbulos Renales Proximales/metabolismo , Aminoacidurias Renales/etiología , Deficiencia de Vitamina D/complicaciones , Vitamina D/metabolismo , 25-Hidroxivitamina D3 1-alfa-Hidroxilasa/genética , 25-Hidroxivitamina D3 1-alfa-Hidroxilasa/metabolismo , Animales , Factor-23 de Crecimiento de Fibroblastos , Predisposición Genética a la Enfermedad , Humanos , Túbulos Renales Proximales/fisiopatología , Proteína 2 Relacionada con Receptor de Lipoproteína de Baja Densidad/genética , Proteína 2 Relacionada con Receptor de Lipoproteína de Baja Densidad/metabolismo , Pronóstico , Receptores de Calcitriol/genética , Receptores de Calcitriol/metabolismo , Receptores de Superficie Celular/genética , Receptores de Superficie Celular/metabolismo , Aminoacidurias Renales/genética , Aminoacidurias Renales/metabolismo , Aminoacidurias Renales/fisiopatología , Factores de Riesgo , Transducción de Señal , Deficiencia de Vitamina D/genética , Deficiencia de Vitamina D/metabolismo , Deficiencia de Vitamina D/fisiopatología , Vitamina D3 24-Hidroxilasa/genética , Vitamina D3 24-Hidroxilasa/metabolismoRESUMEN
GRACILE Syndrome, is an autosomal recessive disease presenting with growth retardation, severe lactic acidosis, Fanconi type tubulopathy, cholestasis, iron overload and early death without any dysmorphological or neurological features. The BCSIL gene mutation is responsible for GRACILE syndrome, Bjornstad syndrome and complex III deficiency. Bjomstad syndrome is characterized by sensorineural hearing loss and abnormal flat twisted hair shafts. The case is GRACILE syndrome with Bjomstad phenotype in neonatal period due to BCSL1 gene mutation.
Asunto(s)
ATPasas Asociadas con Actividades Celulares Diversas/genética , Acidosis Láctica/genética , Colestasis/genética , Análisis Mutacional de ADN , Complejo III de Transporte de Electrones/genética , Retardo del Crecimiento Fetal/genética , Enfermedades del Cabello/genética , Pérdida Auditiva Sensorineural/genética , Hemosiderosis/genética , Errores Innatos del Metabolismo/genética , Enfermedades Mitocondriales/congénito , Fenotipo , Aminoacidurias Renales/genética , Acidosis/diagnóstico , Acidosis/genética , Acidosis Láctica/diagnóstico , Colestasis/diagnóstico , Consanguinidad , Resultado Fatal , Retardo del Crecimiento Fetal/diagnóstico , Enfermedades del Cabello/diagnóstico , Pérdida Auditiva Sensorineural/diagnóstico , Hemosiderosis/diagnóstico , Homocigoto , Humanos , Lactante , Recién Nacido , Masculino , Errores Innatos del Metabolismo/diagnóstico , Enfermedades Mitocondriales/diagnóstico , Enfermedades Mitocondriales/genética , Aminoacidurias Renales/diagnóstico , TurquíaRESUMEN
Coenzyme Q10 deficiency is a clinically and genetically heterogeneous disorder, with manifestations that may range from fatal neonatal multisystem failure, to adult-onset encephalopathy. We report a patient who presented at birth with severe lactic acidosis, proteinuria, dicarboxylic aciduria, and hepatic insufficiency. She also had dilation of left ventricle on echocardiography. Her neurological condition rapidly worsened and despite aggressive care she died at 23 h of life. Muscle histology displayed lipid accumulation. Electron microscopy showed markedly swollen mitochondria with fragmented cristae. Respiratory-chain enzymatic assays showed a reduction of combined activities of complex I+III and II+III with normal activities of isolated complexes. The defect was confirmed in fibroblasts, where it could be rescued by supplementing the culture medium with 10 µM coenzyme Q10. Coenzyme Q10 levels were reduced (28% of controls) in these cells. We performed exome sequencing and focused the analysis on genes involved in coenzyme Q10 biosynthesis. The patient harbored a homozygous c.545T>G, p.(Met182Arg) alteration in COQ2, which was validated by functional complementation in yeast. In this case the biochemical and morphological features were essential to direct the genetic diagnosis. The parents had another pregnancy after the biochemical diagnosis was established, but before the identification of the genetic defect. Because of the potentially high recurrence risk, and given the importance of early CoQ10 supplementation, we decided to treat with CoQ10 the newborn child pending the results of the biochemical assays. Clinicians should consider a similar management in siblings of patients with CoQ10 deficiency without a genetic diagnosis.
Asunto(s)
Transferasas Alquil y Aril/genética , Ataxia/diagnóstico , Ataxia/genética , Mitocondrias Musculares/genética , Enfermedades Mitocondriales/diagnóstico , Enfermedades Mitocondriales/genética , Debilidad Muscular/diagnóstico , Debilidad Muscular/genética , Mutación Puntual , Ubiquinona/análogos & derivados , Ubiquinona/deficiencia , Acidosis Láctica/sangre , Acidosis Láctica/genética , Acidosis Láctica/patología , Transferasas Alquil y Aril/deficiencia , Ataxia/sangre , Ataxia/patología , Consanguinidad , Resultado Fatal , Femenino , Expresión Génica , Insuficiencia Hepática/sangre , Insuficiencia Hepática/genética , Insuficiencia Hepática/patología , Humanos , Recién Nacido , Discapacidad Intelectual/sangre , Discapacidad Intelectual/genética , Discapacidad Intelectual/patología , Mitocondrias Musculares/enzimología , Mitocondrias Musculares/patología , Enfermedades Mitocondriales/sangre , Enfermedades Mitocondriales/patología , Debilidad Muscular/sangre , Debilidad Muscular/patología , Músculo Esquelético/enzimología , Músculo Esquelético/patología , Proteinuria/sangre , Proteinuria/genética , Proteinuria/patología , Aminoacidurias Renales/sangre , Aminoacidurias Renales/genética , Aminoacidurias Renales/patología , Análisis de Secuencia de ADN , Ubiquinona/sangre , Ubiquinona/genéticaRESUMEN
GRACILE syndrome is a rare autosomal recessive disease characterized by fetal growth retardation, Fanconi type aminoaciduria, cholestasis, iron overload, profound lactic acidosis, and early death. It is caused by homozygosity for a missense mutation in the BCS1L gene. The BCS1L gene encodes a chaperone responsible for assembly of respiratory chain complex III. Here we report that a homozygous mutation c.296C > T (p.P99L), in the first exon of BCS1L gene found in an affected 2-month-old boy of asymptomatic consanguineous parents results in GRACILE syndrome. This genotype is associated with a severe clinical presentation. So far no available treatments have changed the fatal course of the disease, and the metabolic disturbance responsible is still not clearly identified. Therefore, providing prenatal diagnosis in families with previous affected infants is of major importance. Mitochondrial disorders are an extremely heterogeneous group of diseases sharing, in common, the fact that they all ultimately impair the function of the mitochondrial respiratory chain. A clinical picture with fetal growth restriction, postnatal lactacidosis, aminoaciduria, hypoglycemia, coagulopathy, elevated liver enzymes, and cholestasis should direct investigations on mitochondrial disorder.
Asunto(s)
Acidosis Láctica/genética , Colestasis/genética , Complejo III de Transporte de Electrones/genética , Retardo del Crecimiento Fetal/genética , Hemosiderosis/genética , Errores Innatos del Metabolismo/genética , Enfermedades Mitocondriales/congénito , Aminoacidurias Renales/genética , ATPasas Asociadas con Actividades Celulares Diversas , Humanos , Lactante , Masculino , Enfermedades Mitocondriales/genética , Mutación MissenseRESUMEN
Our study describes a novel phenotype in a series of nine Saudi patients with lactic acidosis, from four consanguineous families three of which are related. Detailed genetic studies including linkage, homozygosity mapping and targeted sequencing identified a causative mutation in the BCS1L gene. All affected members of the families have an identical mutation in this gene, mutations of which are recognized causes of Björnstad syndrome, GRACILE syndrome and a syndrome of neonatal tubulopathy, encephalopathy, and liver failure (MIM 606104) leading to isolated mitochondrial respiratory chain complex III deficiency. Here we report the appearance of a novel behavioral (five patients) and psychiatric (two patients) phenotype associated with a p.Gly129Arg BCS1L mutation, differing from the phenotype in a previously reported singleton patient with this mutation. The psychiatric symptoms emanated after childhood, initially as hypomania later evolving into intermittent psychosis. Neuroradiological findings included subtle white matter abnormalities, whilst muscle histopathology and respiratory chain studies confirmed respiratory chain dysfunction. The variable neuro-psychiatric manifestations and cortical visual dysfunction are most unusual and not reported associated with other BCS1L mutations. This report emphasizes the clinical heterogeneity associated with the mutation in BCS1L gene, even within the same family and we recommend that defects in this gene should be considered in the differential diagnosis of lactic acidosis with variable involvement of different organs.
Asunto(s)
Acidosis Láctica/genética , Complejo III de Transporte de Electrones/genética , Mutación , ATPasas Asociadas con Actividades Celulares Diversas , Acidosis Láctica/metabolismo , Adolescente , Adulto , Niño , Colestasis/genética , Colestasis/metabolismo , Transporte de Electrón/genética , Complejo III de Transporte de Electrones/metabolismo , Femenino , Retardo del Crecimiento Fetal/genética , Retardo del Crecimiento Fetal/metabolismo , Predisposición Genética a la Enfermedad , Enfermedades del Cabello/genética , Enfermedades del Cabello/metabolismo , Pérdida Auditiva Sensorineural/genética , Pérdida Auditiva Sensorineural/metabolismo , Hemosiderosis/genética , Hemosiderosis/metabolismo , Homocigoto , Humanos , Masculino , Errores Innatos del Metabolismo/genética , Errores Innatos del Metabolismo/metabolismo , Enfermedades Mitocondriales/congénito , Enfermedades Mitocondriales/genética , Enfermedades Mitocondriales/metabolismo , Fenotipo , Aminoacidurias Renales/genética , Aminoacidurias Renales/metabolismoRESUMEN
GRACILE syndrome belongs to the Finnish disease heritage, and is caused by a point mutation in the BCS1L-gene encoding a mitochondrial protein. This leads to dysfunction of the complex III in the respiratory chain. Significant fetal growth disturbance is the primary manifestation. Within the first day the newborn infant develops severe lactic acidosis. Hypoglycemia, elevated serum ferritin and conjugated bilirubin values and aminoaciduria imply mitochondrial liver disease and renal tubulopathy. In Finland, the diagnosis is based on the 232A>G mutation in the BCS1L-gene. No specific treatment is available. GRACILE syndrome leads to early death.
Asunto(s)
Acidosis Láctica/diagnóstico , Colestasis/diagnóstico , Retardo del Crecimiento Fetal/diagnóstico , Hemosiderosis/diagnóstico , Errores Innatos del Metabolismo/diagnóstico , Aminoacidurias Renales/diagnóstico , ATPasas Asociadas con Actividades Celulares Diversas , Acidosis Láctica/epidemiología , Acidosis Láctica/genética , Biomarcadores/sangre , Colestasis/epidemiología , Colestasis/genética , Complejo III de Transporte de Electrones/genética , Retardo del Crecimiento Fetal/epidemiología , Retardo del Crecimiento Fetal/genética , Finlandia/epidemiología , Hemosiderosis/epidemiología , Hemosiderosis/genética , Humanos , Recién Nacido , Errores Innatos del Metabolismo/epidemiología , Errores Innatos del Metabolismo/genética , Enfermedades Mitocondriales/congénito , Mutación Puntual , Aminoacidurias Renales/epidemiología , Aminoacidurias Renales/genéticaRESUMEN
Lysinuric protein intolerance (LPI) is an inherited aminoaciduria caused by defective cationic amino acid transport at the basolateral membrane of epithelial cells in intestine and kidney. LPI is caused by mutations in the SLC7A7 gene, which encodes the y(+)LAT-1 protein, the catalytic light chain subunit of a complex belonging to the heterodimeric amino acid transporter family. LPI was initially described in Finland, but has worldwide distribution. Typically, symptoms begin after weaning with refusal of feeding, vomiting, and consequent failure to thrive. Hepatosplenomegaly, hematological anomalies, neurological involvement, including hyperammonemic coma are recurrent clinical features. Two major complications, pulmonary alveolar proteinosis and renal disease are increasingly observed in LPI patients. There is extreme variability in the clinical presentation even within individual families, frequently leading to misdiagnosis or delayed diagnosis. This condition is diagnosed by urine amino acids, showing markedly elevated excretion of lysine and other dibasic amino acids despite low plasma levels of lysine, ornithine, and arginine. The biochemical diagnosis can be uncertain, requiring confirmation by DNA testing. So far, approximately 50 different mutations have been identified in the SLC7A7 gene in a group of 142 patients from 110 independent families. No genotype-phenotype correlation could be established. Therapy requires a low protein diet, low-dose citrulline supplementation, nitrogen-scavenging compounds to prevent hyperammonemia, lysine, and carnitine supplements. Supportive therapy is available for most complications with bronchoalveolar lavage being necessary for alveolar proteinosis.
Asunto(s)
Riñón/metabolismo , Lisina/orina , Aminoacidurias Renales/genética , Aminoacidurias Renales/metabolismo , Sistema de Transporte de Aminoácidos y+L , Sistemas de Transporte de Aminoácidos/genética , Sistemas de Transporte de Aminoácidos Básicos/genética , Sistemas de Transporte de Aminoácidos Básicos/metabolismo , Células Epiteliales/metabolismo , Finlandia , Cadenas Ligeras de la Proteína-1 Reguladora de Fusión/genética , Cadenas Ligeras de la Proteína-1 Reguladora de Fusión/metabolismo , Estudios de Asociación Genética , Humanos , Mucosa Intestinal/metabolismo , Transportador de Aminoácidos Neutros Grandes 1/genética , Mutación , Proteinosis Alveolar Pulmonar/genética , Proteinosis Alveolar Pulmonar/metabolismo , Aminoacidurias Renales/diagnóstico , Aminoacidurias Renales/dietoterapiaRESUMEN
UNLABELLED: Mitochondrial dysfunction is an important cause for neonatal liver disease. Disruption of genes encoding oxidative phosphorylation (OXPHOS) components usually causes embryonic lethality, and thus few disease models are available. We developed a mouse model for GRACILE syndrome, a neonatal mitochondrial disease with liver and kidney involvement, caused by a homozygous BCS1L mutation (232A>G). This gene encodes a chaperone required for incorporation of Rieske iron-sulfur protein (RISP) into complex III of respiratory chain. Homozygous mutant mice after 3 weeks of age developed striking similarities to the human disease: growth failure, hepatic glycogen depletion, steatosis, fibrosis, and cirrhosis, as well as tubulopathy, complex III deficiency, lactacidosis, and short lifespan. BCS1L was decreased in whole liver cells and isolated mitochondria of mutants at all ages. RISP incorporation into complex III was diminished in symptomatic animals; however, in young animals complex III was correctly assembled. Complex III activity in liver, heart, and kidney of symptomatic mutants was decreased to 20%, 40%, and 40% of controls, respectively, as demonstrated with electron flux kinetics through complex III. In high-resolution respirometry, CIII dysfunction resulted in decreased electron transport capacity through the respiratory chain under maximum substrate input. Complex I function, suggested to be dependent on a functional complex III, was, however, unaffected. CONCLUSION: We present the first viable model of complex III deficiency mimicking a human mitochondrial disorder. Incorporation of RISP into complex III in young homozygotes suggests another complex III assembly factor during early ontogenesis. The development of symptoms from about 3 weeks of age provides a convenient time window for studying the pathophysiology and treatment of mitochondrial hepatopathy and OXPHOS dysfunction in general.
Asunto(s)
Complejo III de Transporte de Electrones/deficiencia , Hepatopatías/genética , Enfermedades Mitocondriales/genética , Chaperonas Moleculares/genética , Mutación/genética , ATPasas Asociadas con Actividades Celulares Diversas , Acidosis Láctica/genética , Animales , Colestasis/genética , Modelos Animales de Enfermedad , Complejo III de Transporte de Electrones/metabolismo , Retardo del Crecimiento Fetal/genética , Hemosiderosis/genética , Homocigoto , Errores Innatos del Metabolismo/genética , Ratones , Ratones Mutantes , Fosforilación Oxidativa , Aminoacidurias Renales/genéticaRESUMEN
Solute carrier family 1, member 1 (SLC1A1; also known as EAAT3 and EAAC1) is the major epithelial transporter of glutamate and aspartate in the kidneys and intestines of rodents. Within the brain, SLC1A1 serves as the predominant neuronal glutamate transporter and buffers the synaptic release of the excitatory neurotransmitter glutamate within the interneuronal synaptic cleft. Recent studies have also revealed that polymorphisms in SLC1A1 are associated with obsessive-compulsive disorder (OCD) in early-onset patient cohorts. Here we report that SLC1A1 mutations leading to substitution of arginine to tryptophan at position 445 (R445W) and deletion of isoleucine at position 395 (I395del) cause human dicarboxylic aminoaciduria, an autosomal recessive disorder of urinary glutamate and aspartate transport that can be associated with mental retardation. These mutations of conserved residues impeded or abrogated glutamate and cysteine transport by SLC1A1 and led to near-absent surface expression in a canine kidney cell line. These findings provide evidence that SLC1A1 is the major renal transporter of glutamate and aspartate in humans and implicate SLC1A1 in the pathogenesis of some neurological disorders.
Asunto(s)
Transportador 3 de Aminoácidos Excitadores/genética , Mutación , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Animales , Línea Celular , Análisis Mutacional de ADN , Perros , Transportador 3 de Aminoácidos Excitadores/química , Transportador 3 de Aminoácidos Excitadores/metabolismo , Femenino , Genes Recesivos , Humanos , Técnicas In Vitro , Discapacidad Intelectual/genética , Discapacidad Intelectual/metabolismo , Riñón/metabolismo , Masculino , Modelos Biológicos , Modelos Moleculares , Datos de Secuencia Molecular , Proteínas Mutantes/química , Proteínas Mutantes/genética , Proteínas Mutantes/metabolismo , Mutación Missense , Oocitos/metabolismo , Linaje , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Aminoacidurias Renales/genética , Aminoacidurias Renales/metabolismo , Eliminación de Secuencia , Homología de Secuencia de Aminoácido , Xenopus laevisRESUMEN
OBJECTIVE: To compare the renal and extra-renal phenotypes of patients classified as having Dent disease, Dent-2 disease, or Lowe syndrome. STUDY DESIGN: Chart review of data from 93 patients with identified voltage-gated chloride channel and chloride/proton antiporter 5 gene and oculo-cerebro-renal syndrome of Lowe gene mutations observed by the authors, complemented with published data. RESULTS: There was a wide overlap of renal symptoms. Nephrocalcinosis was more prevalent in Dent-1 disease, and renal tubular acidosis, aminoaciduria, and renal failure was more prevalent in patients with Lowe syndrome. Patients with Lowe syndrome were shorter than patients with Dent-1 disease, and patients with Dent-2 disease showed an intermediate phenotype. Three patients with Dent-2 disease had mild peripheral cataract, and 9 patients were noted to have some degree of mental retardation. CONCLUSION: There is a phenotypic continuum within patients with Dent-2 disease and Lowe syndrome, suggesting that there are individual differences in the ability to compensate for loss of oculo-cerebro-renal syndrome of Lowe gene function.
Asunto(s)
Síndrome Oculocerebrorrenal/genética , Defectos Congénitos del Transporte Tubular Renal/genética , Acidosis Tubular Renal/genética , Adolescente , Estatura/genética , Catarata/genética , Niño , Preescolar , Canales de Cloruro/genética , Tasa de Filtración Glomerular , Humanos , Lactante , Discapacidad Intelectual/genética , Mutación , Nefrocalcinosis/genética , Fenotipo , Monoéster Fosfórico Hidrolasas/genética , Aminoacidurias Renales/genética , Insuficiencia Renal/genética , Adulto JovenRESUMEN
Inherited aminoacidurias are caused by defective amino-acid transport through renal (reabsorption) and in many cases also small intestinal epithelia (absorption). Recently, many of the genes causing this abnormal transport have been molecularly identified. In this review, we summarize the latest findings in the clinical and molecular aspects concerning the principal aminoacidurias, cystinuria, lysinuric protein intolerance, Hartnup disorder, iminoglycinuria, and dicarboxylic aminoaciduria. Signs, symptoms, diagnosis, treatment, causative or candidate genes, functional characterization of the encoded transporters, and animal models are discussed.
Asunto(s)
Aminoácidos/orina , Aminoacidurias Renales/diagnóstico , Animales , Humanos , Aminoacidurias Renales/genética , Aminoacidurias Renales/metabolismo , Aminoacidurias Renales/terapiaRESUMEN
Lysinuric protein intolerance (LPI) is an inherited aminoaciduria caused by defective cationic amino acid (CAA) transport at the basolateral membrane of epithelial cells in the intestine and kidney. LPI is caused by mutations in the SLC7A7 gene, which encodes the y(+)LAT-1 protein, the catalytic light chain subunit of a complex belonging to the heterodimeric amino acid transporter family. Coexpression of 4F2hc (the heavy chain subunit) and y(+)LAT-1 induces y(+)L activity (CAA transport). So far a total of 43 different mutations of the SLC7A7 gene, nine of which newly reported here, have been identified in a group of 130 patients belonging to at least 98 independent families. The mutations are distributed along the entire gene and include all different types of mutations. Five polymorphisms within the SLC7A7 coding region and two variants found in the 5'UTR have been identified. A genuine founder effect mutation has been demonstrated only in Finland, where LPI patients share the same homozygous mutation, c.895-2A>T. LPI patients show extreme variability in clinical presentation, and no genotype-phenotype correlations have been defined. This phenotypic variability and the lack of a specific clinical presentation have caused various misdiagnoses. At the biochemical level, the elucidation of SLC7A7 function will be necessary to understand precise disease mechanisms and develop more specific and effective therapies. In this review, we summarize the current knowledge of SLC7A7 mutations and their role in LPI pathogenesis.
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Cadenas Ligeras de la Proteína-1 Reguladora de Fusión/genética , Lisina/orina , Mutación , Aminoacidurias Renales/genética , Secuencia de Aminoácidos , Sistema de Transporte de Aminoácidos y+L , Animales , Análisis Mutacional de ADN , Diagnóstico Diferencial , Cadenas Ligeras de la Proteína-1 Reguladora de Fusión/fisiología , Genotipo , Humanos , Modelos Animales , Datos de Secuencia Molecular , Fenotipo , Polimorfismo Genético , Aminoacidurias Renales/metabolismoRESUMEN
Arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome is a rare multisystem disorder first described in 1979 and recently ascribed to mutation in VPS33B, whose product acts in intracellular trafficking. Arthrogryposis, spillage of various substances in the urine, and conjugated hyperbilirubinemia define an ARC core phenotype, in some patients associated with ichthyosis, central nervous system malformation, deafness, and platelet abnormalities. We describe a patient with cholestasis, aminoaciduria, ichthyosis, partial callosal agenesis, and sensorineural deafness who, although homozygous for the novel VPS33B mutation 971delA/K324fs, predicted to abolish VPS33B function, did not exhibit arthrogryposis. The phenotypes associated with VPS33B mutation may include incomplete ARC.
Asunto(s)
Colestasis/diagnóstico , Ictiosis/diagnóstico , Enfermedades Renales/diagnóstico , Agenesia del Cuerpo Calloso , Artrogriposis/genética , Colestasis/genética , Resultado Fatal , Femenino , Pérdida Auditiva Sensorineural/diagnóstico , Pérdida Auditiva Sensorineural/genética , Humanos , Hiperbilirrubinemia/etiología , Ictiosis/genética , Lactante , Enfermedades Renales/genética , Proteínas de la Membrana/genética , Mutación , Fenotipo , Aminoacidurias Renales/diagnóstico , Aminoacidurias Renales/genética , Síndrome , Proteínas de Transporte VesicularRESUMEN
Heteromeric amino acid transporters (HATs) are composed of a heavy (SLC3 family) and a light (SLC7 family) subunit. Mutations in system b(0,+) (rBAT-b(0,+)AT) and in system y(+)L (4F2hc-y(+)LAT1) cause the primary inherited aminoacidurias (PIAs) cystinuria and lysinuric protein intolerance, respectively. Recent developments [including the identification of the first Hartnup disorder gene (B0AT1; SLC6A19)] and knockout mouse models have begun to reveal the basis of renal and intestinal reabsorption of amino acids in mammals.
Asunto(s)
Sistemas de Transporte de Aminoácidos/genética , Proteínas Portadoras/química , Proteínas Portadoras/genética , Cistinuria/genética , Aminoacidurias Renales/genética , Animales , Cistinuria/fisiopatología , Humanos , Aminoacidurias Renales/fisiopatologíaRESUMEN
BACKGROUND: Familial renal glucosuria (FRG) is an isolated disorder of proximal tubular glucose transport, characterized by abnormal urinary glucose excretion in the presence of normal blood glucose levels. Generalized aminoaciduria has not generally been considered a feature of this disorder. FRG has recently been shown to result from mutations in SLC5A2, encoding the kidney-specific low-affinity/high-capacity Na+/glucose cotransporter, SGLT2. The purpose of this study was to examine the phenotypic and genetic characteristics of three unrelated consanguineous families with FRG accompanied by aminoaciduria. METHODS: Six children with autosomal-recessive FRG and 12 unaffected family members were evaluated at the clinical and molecular levels. DNA sequence analysis of the entire coding sequence of SLC5A2 was performed in all affected individuals. Haplotype analysis using four polymorphic markers flanking SLC5A2 was performed in all study participants. RESULTS: All affected children were asymptomatic, but displayed massive glucosuria (83 to 169 g/1.73 m(2)/day) accompanied by generalized aminoaciduria. Sequence analysis in all patients revealed a novel homozygous missense mutation in exon 8 of SLC5A2, resulting in a lysine to arginine substitution at position 321 of SGLT2 amino acid sequence (K321R). The mutation was confirmed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and was found to completely cosegregate with the FRG phenotype. Haplotype analysis is consistent with identity by descent for the mutation. The K321 residue, presumed to be located in the eighth transmembrane domain of SGLT2, is highly conserved across SGLT homologues. CONCLUSION: Our findings confirm that mutations in SLC5A2 result in autosomal-recessive FRG. The severe glucosuria in homozygotes for the K321R mutation highlights the importance of the eighth SGLT2 transmembrane domain for normal glucose transport. We suggest that the generalized aminoaciduria accompanying FRG is a consequence of the severe impairment in glucose reabsorption, and is probably not directly related to the SGLT2 mutation. The exact role of the aberrant glucose transport in the pathogenesis of aminoaciduria remains to be established.
Asunto(s)
Glucosuria Renal/genética , Proteínas de Transporte de Monosacáridos/genética , Mutación Missense , Aminoacidurias Renales/genética , Secuencia de Aminoácidos , Árabes/genética , Secuencia de Bases , Niño , Preescolar , Consanguinidad , ADN/genética , Femenino , Genes Recesivos , Haplotipos , Humanos , Lactante , Israel , Masculino , Datos de Secuencia Molecular , Linaje , Fenotipo , Polimorfismo de Longitud del Fragmento de Restricción , Homología de Secuencia de Aminoácido , Transportador 2 de Sodio-GlucosaRESUMEN
In lysinuric protein intolerance (LPI), impaired transport of cationic amino acids in kidney and intestine is due to mutations of the SLC7A7 gene. To assess the functional consequences of the LPI defect in nonepithelial cells, we have characterized cationic amino acid (CAA) transport in human fibroblasts obtained from LPI patients and a normal subject. In both cell types the bidirectional fluxes of arginine are due to the additive contributions of two Na(+)-independent, transstimulated transport systems. One of these mechanisms, inhibited by N-ethylmaleimide (NEM) and sensitive to the membrane potential, is identifiable with system y(+). The NEM- and potential-insensitive component, suppressed by L-leucine only in the presence of Na(+), is mostly due to the activity of system y(+)L. The inward and outward activities of the two systems are comparable in control and LPI fibroblasts. Both cell types express SLC7A1 (CAT1) and SLC7A2 (CAT2B and CAT2A) as well as SLC7A6 (y+LAT2) and SLC7A7 (y+LAT1). We conclude that LPI fibroblasts exhibit normal CAA transport through system y(+)L, probably referable to the activity of SLC7A6/y+LAT2.