1.
Clin Transl Med
; 11(11): e572, 2021 11.
Artículo
en Inglés
| MEDLINE
| ID: mdl-34841699
Asunto(s)
Aminoácido Oxidorreductasas/farmacología , Antifibróticos/farmacología , Técnicas Biosensibles/instrumentación , Técnicas Biosensibles/normas , Aminoácido Oxidorreductasas/análisis , Aminoácido Oxidorreductasas/uso terapéutico , Antifibróticos/análisis , Antifibróticos/uso terapéutico , Técnicas Biosensibles/métodos , Fibrosis/tratamiento farmacológico , Fibrosis/prevención & control , Humanos
2.
STAR Protoc
; 2(1): 100387, 2021 03 19.
Artículo
en Inglés
| MEDLINE
| ID: mdl-33778781
RESUMEN
Controversies remain over the standard procedures for the modeling of skin fibrosis and its use in in vitro testing of different drugs. Here, we report a reproducible protocol for producing a skin fibrosis model using human dermal fibroblasts seeded in collagen hydrogel. Detailed procedures for the fabrication of cell/hydrogel constructs, fibrosis induction, protein extraction for western blotting analysis are presented along with how this model can be employed for investigating the possible anti-fibrotic functions of certain chemical compounds.
