RESUMEN
SUMMARY: One of the reasons for acute kidney damage is renal ischemia. Nevertheless, there are limited protective and therapeutic approaches for this problem. Diacerein is an anti-inflammatory drug characterized by numerous biological activities. We aimed to determine the ameliorative impact of diacerein on renal ischemia/reperfusion injury (I/R) condition, exploring the underlying mechanisms. Twenty-four male rats were allotted into four groups (n= 6): sham group; Diacerein (DIA) group; I/R group, in which a non-crushing clamp occluded the left renal pedicle for 45 min, and the right kidney was nephrectomized for 5 min before the reperfusion process; I/R + diacerein group, injected intraperitoneally with 50 mg diacerein/kg i.m 30 minutes prior to I/R operation. Ischemia/ reperfusion was found to affect renal function and induce histopathological alterations. The flow cytometry analysis demonstrated an elevated expression of innate and mature dendritic cells in I/R renal tissues. Moreover, upregulation in the expression of the inflammatory genes (TLR4, Myd88, and NLRP3), and overexpression of the pro-inflammatory cytokines (IL-1β), apoptotic (caspase-3) and pyroptotic (caspase-1) markers were observed in I/R-experienced animals. The aforementioned deteriorations were mitigated by pre-I/R diacerein treatment. Diacerein alleviated I/R-induced inflammation and apoptosis. Thus, it could be a promising protective agent against I/R.
La isquemia renal es una de los motivos del daño renal agudo. Sin embargo, los enfoques protectores y terapéuticos para este problema son limitados. La diacereína es un fármaco antiinflamatorio caracterizado por numerosas actividades biológicas. Nuestro objetivo fue determinar el impacto de mejora de la diacereína en la condición de lesión por isquemia/ reperfusión renal (I/R), explorando los mecanismos subyacentes. Veinticuatro ratas macho se distribuyeron en cuatro grupos (n= 6): grupo simulado; grupo de diacereína (DIA); grupo I/R, en el que una pinza no aplastante ocluyó el pedículo renal izquierdo durante 45 min, y el riñón derecho fue nefrectomizado durante 5 min antes del proceso de reperfusión; Grupo I/R + diacereína, inyectado por vía intraperitoneal con 50 mg de diacereína/kg i.m. 30 min antes de la operación I/R. Se encontró que la isquemia/ reperfusión afecta la función renal e induce alteraciones histopatológicas. El análisis de citometría de flujo demostró una expresión elevada de células dendríticas innatas y maduras en tejidos renales I/R. Además, se observó una regulación positiva en la expresión de los genes inflamatorios (TLR4, Myd88 y NLRP3) y una sobreexpresión de las citoquinas proinflamatorias (IL-1β), marcadores apoptóticos (caspasa-3) y piroptóticos (caspasa-1) en animales con experiencia en I/R. Los deterioros antes mencionados fueron mitigados por el tratamiento previo a la diacereína I/R. La diacereína alivió la inflamación y la apoptosis inducidas por I/R. Por lo tanto, podría ser un agente protector prometedor contra I/R.
Asunto(s)
Animales , Ratas , Daño por Reperfusión/tratamiento farmacológico , Antraquinonas/administración & dosificación , Enfermedades Renales/tratamiento farmacológico , Antiinflamatorios/administración & dosificación , Células Dendríticas/efectos de los fármacos , Daño por Reperfusión/inmunología , Transducción de Señal , FN-kappa B/metabolismo , Antraquinonas/inmunología , Apoptosis/efectos de los fármacos , Estrés Oxidativo , Receptor Toll-Like 4/metabolismo , Interleucina-1beta/metabolismo , Citometría de Flujo , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Inflamación , Inyecciones Intraperitoneales , Enfermedades Renales/inmunologíaRESUMEN
The Rhei Radix et Rhizoma was one of the most widely used traditional Chinese medicine for its special biological activities. The content of rhein, one of its major compounds, was an important standard for the quantity control of Rhei Radix et Rhizoma. The major method used for the detection of rhein was instrumental analysis like HPLC, but it was complex, time-consuming and cannot detect large samples at the same time. The enzyme-linked imunmosorbent assay (ELISA) was accurate, reliable, simple, low costs, and of a high-throughout. Recently, it was widely used for the determination of those small molecule compounds in some traditional Chinese medicinal plants. In this study, an artificial antigen were synthesized by the carbodiimide (CDI) method. Rhein-bovine (rhein-BSA) conju gate and rhein-ovalbumin (rhein-OVA) conjugate, were produced as the immunogen and coating antigen, respectively. The conjugate and the hapten number in the conjugate were determined by UV-Vis spectrophotometry (UV). The conjugation ratio of Rhein and BSA was about 4.0:1, rhein acid and OVA was 2.6 : 1, respectively. Rhein-BSA conjugate was used to immunize Bal b/c mice to produce antiserum. The antiserum titer of the Rhein were higher than 8000 detected by ELISA. The successfully synthesized conjugate antigen rhein-BSA implies its feasibility in the establishment of fast immunoassay for the rhein content determination.
Asunto(s)
Antraquinonas/análisis , Antígenos de Plantas/análisis , Medicamentos Herbarios Chinos/análisis , Ensayo de Inmunoadsorción Enzimática/métodos , Rheum/química , Animales , Antraquinonas/inmunología , Anticuerpos/análisis , Anticuerpos/inmunología , Antígenos de Plantas/inmunología , Ensayo de Inmunoadsorción Enzimática/instrumentación , Ratones , Ratones Endogámicos BALB C , Rheum/inmunología , Rizoma/química , Rizoma/inmunologíaRESUMEN
INTRODUCTION: Rhubarb, senna and sennoside-containing preparations are currently widely employed as purgatives. The major active components of these medications are sennoside A (SA) and sennoside B (SB). OBJECTIVE: To develop an eastern blotting technique for the specific visualisation and easy determination of SA and SB in plant extracts for application in the standardisation and authentication of rhubarb and senna. METHODOLOGY: SA and SB were separated by TLC, transferred to a PVDF membrane, treated with 1-ethyl-3-(3'-dimethylaminopropyl)-carbodiimide hydrochloride solution and finally treated with bovine serum albumin (BSA). The resulting membrane-bound SA-BSA and SB-BSA conjugates were linked to anti-SA and anti-SB monoclonal antibodies (MAbs) and then to secondary antibodies labelled with peroxidase. SA and SB were detected by visualisation of the peroxidase reaction products. RESULTS: The limit of detection of the eastern blotting was 62.5 ng for both sennosides. The method was applied to the immunohistochemical localisation of SA in fresh rhubarb root. Phloem and radiate wood were found to contain higher concentrations of SA compared with other tissues (pith and bud) in agreement with results obtained by ELISA. The concentrations of SA in the phloem, radiate wood, pith and bud were 64.4, 48.1, 15.0 and 1.8 ng/mg fresh weight, respectively. CONCLUSION: The technique described permitted the visualisation of small molecular weight compounds that had been bound to a membrane, using immunostaining. Owing to the specificity of the MAbs, the eastern blotting may prove to be a useful method for the identification of SA and SB in a background containing large amount of impurities.
Asunto(s)
Antraquinonas/análisis , Anticuerpos Monoclonales/inmunología , Immunoblotting/métodos , Antraquinonas/inmunología , Cromatografía en Capa Delgada , Ensayo de Inmunoadsorción Enzimática , Inmunohistoquímica , Extracto de Senna , SenósidosRESUMEN
Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.
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Antraquinonas/análisis , Cassia/química , Catárticos/análisis , Ensayo de Inmunoadsorción Enzimática/métodos , Rheum/química , Senna/química , Antraquinonas/inmunología , Anticuerpos Monoclonales , Reproducibilidad de los Resultados , Extracto de Senna/química , SenósidosAsunto(s)
Antraquinonas/administración & dosificación , Anticuerpos Antifúngicos/análisis , Catárticos/administración & dosificación , Enfermedades Inflamatorias del Intestino/inmunología , Saccharomyces cerevisiae/inmunología , Antraquinonas/inmunología , Formación de Anticuerpos , Biomarcadores/análisis , Reacciones Falso Positivas , HumanosAsunto(s)
Anticuerpos Monoclonales , Cromatografía/métodos , Medicamentos Herbarios Chinos/química , Ensayo de Inmunoadsorción Enzimática/métodos , Inmunoensayo/métodos , Animales , Antraquinonas/análisis , Antraquinonas/inmunología , Ácido Glicirrínico/análisis , Ácido Glicirrínico/inmunología , Immunoblotting/métodos , Juego de Reactivos para Diagnóstico , Extracto de Senna , Senósidos , Sensibilidad y EspecificidadRESUMEN
DEGRADATION AND REPAIR: Osteoarthritis generally occurs in a context of an overloaded normal cartilage matrix or normal loading of a vulnerable cartilage matrix. Interleukin-1 appears to be principally indicated in degradation phenomena while transforming growth factor (TGFbeta) is mainly implicated in phenomena of excessive synovial and chondrocyte repair observed at the same time as degradation. MECHANISMS OF ACTION OF DIACEREIN: Diacerein is a slow acting symptomatic treatment of osteoarthritis which has demonstrated efficacy on functional manifestations of osteoarthritis and on the structural component. Two mechanisms of action have been validated: in vitro inhibition of interleukin-1 (IL-1) synthesis, the main cytokine involved in cartilage destruction, and activity on the synthesis of proteoglycans, and hyaluronic acid, the principal component of cartilage. Other studies presented here have provided further details concerning other mechanisms of action of diacerein.
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Antraquinonas/uso terapéutico , Antiinflamatorios no Esteroideos/uso terapéutico , Interleucina-1/antagonistas & inhibidores , Osteoartritis/tratamiento farmacológico , Animales , Antraquinonas/inmunología , Antraquinonas/farmacología , Antiinflamatorios no Esteroideos/inmunología , Antiinflamatorios no Esteroideos/farmacología , Condrocitos/efectos de los fármacos , Condrocitos/inmunología , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Evaluación Preclínica de Medicamentos , Humanos , Ácido Hialurónico/fisiología , Osteoartritis/inmunología , Osteoartritis/patología , Proteoglicanos/efectos de los fármacos , Proteoglicanos/fisiología , Ensayos Clínicos Controlados Aleatorios como Asunto , Factores de Crecimiento Transformadores/inmunología , Resultado del TratamientoRESUMEN
We describe here a new type of method for isolation of rare cell populations in biological fluids. The method is based on the anthraquinone technology for covalent binding of molecules to a polymer surface. An anthraquinone molecule conjugated via a linker to an electrophilic group (AQ Immobilizer trade mark reagent, Exiqon A/S) is covalently bound to a polymer surface by UV irradiation. The electrophilic group of this AQ reagent can covalently bind a specific antibody directed against a specific cell marker. Applying a cell sample to the functional surface, the cells having the specific cell marker on the cell surface will bind to the antibody on the functional surface. Using this technique, even extremely small cell populations may be isolated. We succeeded in isolating fetal cells from maternal blood samples in the first trimester for chromosome defects genetic diagnosis.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Separación Celular/métodos , Sangre Fetal/inmunología , Transfusión Fetomaterna/inmunología , Embarazo/sangre , Diagnóstico Prenatal/métodos , Adulto , Antraquinonas/química , Antraquinonas/inmunología , Eritroblastos/citología , Eritroblastos/inmunología , Femenino , Sangre Fetal/citología , Edad Gestacional , Humanos , Hibridación Fluorescente in Situ , Recién Nacido , Masculino , Fotoquímica , Polímeros/química , Reproducibilidad de los Resultados , Propiedades de SuperficieRESUMEN
Proteins are renowned for their specificity of function. There is, however, accumulating evidence that many proteins, from enzymes to antibodies, are functionally promiscuous. Promiscuity is of considerable physiological importance. In the immune system, cross-reactive or multispecific antibodies are implicated in autoimmune and allergy conditions. In most cases, however, the mechanism behind promiscuity and the relationship between specific and promiscuous activities are unknown. Are the two contradictory? Or can a protein exhibit several unrelated activities each of which is highly specific? To address these questions, we studied a multispecific IgE antibody (SPE7) elicited against a 2,4-dinitrophenyl hapten (DNP). SPE7 is able to distinguish between closely related derivatives such as NP (nitrophenol) and DNP, yet it can also bind a number of unrelated ligands. We find that, like DNP, the cross-reactants are themselves bound specifically-close derivatives of these cross-reactants show very low or no binding to SPE7. It has been suggested that cross-reactivity is simply due to "hydrophobic stickiness", nonspecific interactions between hydrophobic ligands and binding sites. However, partitioning experiments reveal that affinity for SPE7 is unrelated to ligand hydrophobicity. These data, combined with crystal structures of SPE7 in complex with four different ligands, demonstrate that each cross-reactant is bound specifically, forming different hydrogen bonds dependant upon its particular chemistry and the availability of complementary antibody residues. SPE7 is highly homologous to the germline antinitrophenol (NP) antibody B1-8. By comparing the sequences and binding patterns of SPE7 and B1-8, we address the relationship between affinity maturation, specificity, and cross-reactivity.
Asunto(s)
Reacciones Cruzadas/inmunología , Enlace de Hidrógeno , Interacciones Hidrofóbicas e Hidrofílicas , Secuencia de Aminoácidos , Animales , Antracenos/química , Antraquinonas/química , Antraquinonas/inmunología , Afinidad de Anticuerpos/inmunología , Especificidad de Anticuerpos/inmunología , Secuencia de Bases , Unión Competitiva/inmunología , Clonación Molecular , Cristalografía por Rayos X , Dinitrofenoles/química , Dinitrofenoles/inmunología , Ensayo de Inmunoadsorción Enzimática/métodos , Haptenos/química , Haptenos/inmunología , Inmunoglobulina E/química , Inmunoglobulina E/inmunología , Fragmentos Fab de Inmunoglobulinas/genética , Fragmentos Fab de Inmunoglobulinas/inmunología , Región Variable de Inmunoglobulina/genética , Región Variable de Inmunoglobulina/inmunología , Ratones , Modelos Moleculares , Datos de Secuencia Molecular , Estructura Molecular , Nitrofenoles/química , Nitrofenoles/inmunología , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Bases de Schiff/química , Análisis de Secuencia de ADN , Homología de Secuencia de AminoácidoRESUMEN
For immunization, sennoside B was conjugated with bovine serum albumin. The hapten density in the antigen conjugate was determined to be 3 mol mol(-1) protein by matrix-assisted laser desorption-ionization TOF mass spectrometry. A hybridoma secreting monoclonal antibody against sennoside B was produced by fusing splenocytes from mouse immunized with the sennoside B conjugate and mouse myeloma cells. Weak cross-reactivities occurred with sennoside A which is a stereochemical isomer, and a monomer of sennoside B, rhein, but no cross-reactivity was observed with other related anthraquinones and phenolics. The range of the assay extended from 0.5 ng ml(-1) to 15 ng ml(-1) of sennoside B, and good correlation between ELISA and HPLC methods was obtained when crude extracts of rhubarb were analyzed.
Asunto(s)
Antraquinonas/inmunología , Anticuerpos Monoclonales/aislamiento & purificación , Catárticos/análisis , Antraquinonas/análisis , Ensayo de Inmunoadsorción Enzimática/métodos , Extracto de Senna , SenósidosRESUMEN
In this study we demonstrate a new UV irradiation technique for covalent coupling of bacterial polysaccharides derived from lipopolysaccharides to microtiter plates and the use of such plates in an enzyme linked immunosorbent assay (ELISA). Lipopolysaccharides were cleaved by mild acid hydrolysis into the lipid A part and the polysaccharide part. The polysaccharide was conjugated regiospecifically to a photochemically active compound, anthraquinone, resulting in a polysaccharide-anthraquinone conjugate. Anthraquinones forms active radicals when exposed to soft UV irradiation (350 nm) permitting the formation of stable covalent bonds to polymers e.g. microtiter plates. By this technique the polysaccharides are bound through the anthraquinone part of the polysaccharide-anthraquinone conjugates to the microtiter plates. This minimizes denaturation of O-antigen epitopes during binding to the microtiter plates and avoids cross-reactivity due to conserved domains in the lipid A. Furthermore, the covalent binding of the polysaccharide antigens are compatible with harsh assay conditions, such as extensive washing procedures and buffers with high salt concentrations with no risk of antigen leakage. Here we describe the use of this technique for the immobilization of lipopolysaccharide derived polysaccharides from Salmonella Typhimurium and Salmonella Choleraesuis lipopolysaccharides, representing the O-antigens 1, 4, 5, 6, 7, and 12. The functional polysaccharide surface gave similar ELISA results to plates coated passively with the corresponding unmodified lipopolysaccharide antigens. The plates were highly reproducible, showed very low inter- and intra-plate variation and were stable at room temperature for more than 8 months.
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Anticuerpos Antibacterianos/sangre , Ensayo de Inmunoadsorción Enzimática/métodos , Lipopolisacáridos/inmunología , Salmonelosis Animal/inmunología , Salmonella typhimurium/aislamiento & purificación , Animales , Antraquinonas/inmunología , Anticuerpos Antibacterianos/inmunología , Lipopolisacáridos/análisis , Reproducibilidad de los Resultados , Salmonelosis Animal/sangre , Salmonella typhimurium/inmunología , Porcinos , Rayos UltravioletaRESUMEN
A sensitive and specific radioimmunoassay for mitoxantrone in serum has been developed. The procedure allows direct measurement of mitoxantrone in unextracted serum samples, by using antisera from rabbits immunized with mitoxantrone-BSA antigen. Tritiated mitoxantrone of high specific radioactivity (ca. 15 Ci/mmol) was used as a radio-tracer ligand. The assay allows the detection of as little as 50 pg/ml and the quantitation of 75 pg/ml in 0.5 ml serum samples. Standard curves were linear in the concentration range of 75-2500 pg/ml, at antiserum dilutions of 1:15,000. The assay shows good reproducibility: coefficients of variation of 3-6% were obtained by analyzing five samples/concentration at 75, 100, 250, 500, and 1000 pg/ml. There was no cross reactivity with the major metabolite in human serum, having concentrations of up to 10,000 pg/ml. Serum samples collected at various time intervals from rats dosed intravenously with mitoxantrone (0.5 mg/kg), were analyzed for unchanged mitoxantrone by RIA. The drug concentrations decreased from 32 ng/ml at 0.5 h to 0.45 ng/ml by 24 h after dosing. Mitoxantrone, a dihydroxyanthracenedione derivative (1), is an antitumor agent currently used in clinical trials with very encouraging results, especially in metastatic breast cancer, and low incidence of adverse reactions (2-4). The drug is being administered intravenously at doses up to 14 mg/m2. Preliminary pharmacokinetic studies (to be published separately) indicate rapid distribution, followed by slow clearance rates from the tissues. The sensitivity of the currently available (HPLC) methods (5, 6) is of about 5-20 ng/ml in serum.(ABSTRACT TRUNCATED AT 250 WORDS)