RESUMEN
A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium designated strain C605018/01/1T isolated from a milk sample collected from the udder of a cow at post mortem. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strain of Arcanobacterium pluranimalium (99.76 %); sequence similarities to all other Arcanobacterium species were below 97â%. The wet-lab DNA-DNA hybridization values among strain C605018/01/1T and A. pluranimalium DSM 13483áµ were low, 16.9â% (reciprocal, 49.8 %). Pertaining to the whole genome sequence with a total length of 2.02 Mb and 1654 protein counts, the novel strain C605018/01/01T displayed a G+C content of 51.6 % mol%. The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol-mannoside and unidentified glycolipid and aminophospholipids. Based on these results it is proposed that strain C605018/01/1T should be classified as representing a novel species, Arcanbacterium bovis sp. nov. The type strain C605018/01/1T (CCUG 45425T=DSM 107286T=BCCM/LMG 30783T).
Asunto(s)
Arcanobacterium/clasificación , Mastitis Bovina/microbiología , Leche/microbiología , Filogenia , Animales , Arcanobacterium/aislamiento & purificación , Técnicas de Tipificación Bacteriana , Composición de Base , Bovinos , ADN Bacteriano/genética , Ácidos Grasos/química , Femenino , Glucolípidos/química , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/química , Secuenciación Completa del GenomaRESUMEN
INTRODUCTION: Aging and comorbidities such as diabetes and vascular problems contribute to the increasing occurrence of chronic wounds. From the beginning of 2016, a marked increase in Arcanobacterium haemolyticum (ARH) in chronic wound cultures was noted among patients visiting a wound expertise centre in The Netherlands. AIM: To report the outbreak investigation of ARH cultured from chronic wounds and describe the implemented infection prevention measures. METHODS: In total, 50 ARH isolates were sent to a reference laboratory for molecular typing. Samples for bacterial culture and ARH polymerase chain reaction were taken from care workers, the environment and items used for wound care. Infection prevention measures were implemented in a bundled approach, involving education, better aseptic wound care conditions and hygienic precautions. Before and after the implementation of infection prevention measures, two screening rounds of ARH testing were performed among all patients receiving home care. RESULTS: ARH isolates from wound care patients were found to be identical by core genome multi-locus sequence typing. No definite outbreak source could be determined by culture. However, three pairs of forceps, used by two nurses on multiple patients, were found to be ARH positive by polymerase chain reaction. In the two screening rounds before and after the implementation of infection prevention measures, the proportion of ARH-positive patients decreased significantly from 20% (20/99) to 3% (3/104). Subsequently, no new cases occurred. CONCLUSION: This first ARH outbreak was likely caused by re-using contaminated instruments. Through the implementation of improved infection prevention measures and re-education of all employees involved, the outbreak was controlled. With the current trend of care transition, infection control must be a major concern.
Asunto(s)
Infecciones por Actinomycetales/epidemiología , Arcanobacterium/genética , Brotes de Enfermedades , Control de Infecciones/métodos , Infección de Heridas/microbiología , Arcanobacterium/clasificación , Bacteriemia/epidemiología , Enfermedad Crónica/epidemiología , Implementación de Plan de Salud , Humanos , Pierna/microbiología , Pierna/patología , Tipificación de Secuencias Multilocus , Países Bajos/epidemiología , Estudios Retrospectivos , Infección de Heridas/complicaciones , Infección de Heridas/epidemiologíaRESUMEN
The present study was designed to identify nine Arcanobacterium phocae strains isolated from cases of mink dermatitis of a single farm in Finland and characterize the strains for epidemiological relationships. All nine strains and previously described A. phocae used for comparative purposes were identified and further characterized phenotypically, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), by Fourier Transform Infrared Spectroscopy (FT-IR) and genotypically by detection of phocaelysin encoding gene phl with a previously developed loop-mediated isothermal amplification (LAMP) assay and by sequencing 16S rRNA gene and gene phl, the elongation factor tu encoding gene tuf and the ß subunit of bacterial RNA polymerase encoding gene rpoB. Genetic relatedness among isolates was determined using whole-genome single nucleotide polymorphism (wgSNP) analysis. The wgSNP results, partly the MALDI-TOF MS and FT-IR analyses and sequencing of the genes, revealed that the nine A. phocae strains recovered from a single farm showed close sequence similarities among each other and differed from previously investigated A. phocae strains isolated from other farms and animals in Finland and from the A. phocae type strain. This indicated a close epidemiological relationship of the A. phocae strains isolated from a single farm and that the nine A. phocae strains of the present study might have developed from a common ancestor.
Asunto(s)
Infecciones por Actinomycetales/epidemiología , Infecciones por Actinomycetales/veterinaria , Arcanobacterium/genética , Dermatitis/epidemiología , Dermatitis/veterinaria , Visón/microbiología , Animales , Arcanobacterium/clasificación , Dermatitis/microbiología , Granjas , Finlandia/epidemiología , Genoma Bacteriano , Genotipo , Fenotipo , Filogenia , Polimorfismo de Nucleótido Simple , ARN Ribosómico 16S/genética , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
The newly described type strains Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, initially isolated from an anal swab of a harbor seal (Sammra et al. Int J Syst Evol Microbiol 65:4539-4543, 2015) and the genital tract of a rhinoceros (Sammra et al. Int J Syst Evol Microbiol 67:2093-2097, 2017), could be further characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and Fourier transform infrared (FT-IR) spectroscopy and by sequencing the genomic targets 16S-23S rDNA intergenic spacer region (ISR) and the genes rpoB, gap, and tuf. The two strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of both species remained unclear. However, the detection of specific spectra by MALDI-TOF MS and by FT-IR spectroscopy and the presented genotypic approaches might help to identify A. pinnipediorum and A. wilhelmae in the future and might elucidate the role these two species play in infections of animals.
Asunto(s)
Arcanobacterium/clasificación , Arcanobacterium/genética , Arcanobacterium/aislamiento & purificación , ADN Bacteriano , ADN Ribosómico/genética , ADN Espaciador Ribosómico/genética , Filogenia , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
In the present study 12 Arcanobacterium phocae strains isolated from fur animals in Finland, including foxes, minks and Finnraccoons, could successfully be identified phenotypically, by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and phocaelysin (PHL) encoding gene phl. The PHL of all 12 A. phocae strains in the present study and reference strains A. phocae DSM 10002T and A. phocae DSM 10003 displayed, as typical members of the cholesterol dependent cytolysin-group of toxins, the variant undecapeptide sequence EATGLAWDPWW which appeared to be most closely related to arcanolysin of Arcanobacterium haemolyticum and pyolysin of Trueperella pyogenes. In addition, gene phl could be determined with a newly designed loop-mediated isothermal amplification (LAMP) assay. The detection of mass spectra by MALDI-TOF MS and the LAMP assay based on gene phl might help to reliably identify A. phocae in future and also elucidate the role this species plays in infections of fur animals.
Asunto(s)
Infecciones por Actinomycetales/veterinaria , Arcanobacterium/genética , Arcanobacterium/aislamiento & purificación , Proteínas Bacterianas/genética , Fenotipo , Infecciones por Actinomycetales/microbiología , Animales , Arcanobacterium/clasificación , Finlandia/epidemiología , Zorros/microbiología , Genotipo , Visón/microbiología , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN/métodos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodosRESUMEN
A taxonomic study using a polyphasic approach was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium isolated from the genital tract of a rhinoceros. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium canis (98.8â% 16S rRNA gene sequence similarity), Arcanobacterium phocisimile (97.8â%), Arcanobacterium phocae (97.7â%), Arcanobacterium haemolyticum (97.4â%), Arcanobacterium hippocoleae (96.6â%), Arcanobacterium pinnipediorum (96.4â%) and Arcarnobacterium pluranimalium (95.4â%). DNA-DNA hybridization values between strain 647T and Arcanobacterium canisDSM 25104T were very low, 13.4â% (reciprocal 15.9â%). The genomic DNA G+C content of strain 647T was 58.7 mol%. The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine and an unidentified phosphoglycolipid. The results of physiological and biochemical testing clearly distinguished the unknown bacterium from other species of the genus Arcanobacterium. Based on these tests, it is proposed that the unknown bacterium should be classified as a representative of a novel species of the genus Arcanobacterium named Arcanobacterium wilhelmaesp. nov. The type strain is 647T (=DSM 102162T=LMG 29418T).
Asunto(s)
Arcanobacterium/clasificación , Perisodáctilos/microbiología , Filogenia , Sistema Urogenital/microbiología , Animales , Arcanobacterium/genética , Arcanobacterium/aislamiento & purificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Femenino , Alemania , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
BACKGROUND: The emerging skin disease fur animal epidemic necrotic pyoderma (FENP) has been attributed to infection with Arcanobacterium phocae (ABP). The exact pathogenesis and risk factors of FENP have yet to be elucidated. ANIMALS: Three mink from each of three different mink farms (A-C) with postvaccination skin wounds at the vaccination site and six mink from an unaffected mink farm (D) that had used the same vaccine batch and vaccination site (hind leg). METHODS AND RESULTS: All mink from farms A-C had severe necrotizing to necropurulent dermatitis where they were vaccinated intramuscularly in the hind leg. ABP was the sole bacterium cultured from six of nine wounds. Using 16S-23S rDNA intergenic spacer region and BOX-PCR, the ABP isolates from these wounds were indistinguishable from isolates originating from several cases of FENP. CONCLUSIONS AND CLINICAL IMPORTANCE: This is the first report of FENP-like lesions at the site of vaccination, in the days following the procedure, associated with ABP. At farms with FENP vaccination, procedures should be considered carefully.
Asunto(s)
Infecciones por Actinomycetales/veterinaria , Arcanobacterium/clasificación , Visón , Vacunación/efectos adversos , Infección de Heridas/veterinaria , Heridas y Lesiones/veterinaria , Infecciones por Actinomycetales/microbiología , Infecciones por Actinomycetales/patología , Animales , Enfermedades Transmisibles Emergentes/veterinaria , Enfermedades Cutáneas Bacterianas/microbiología , Enfermedades Cutáneas Bacterianas/patología , Enfermedades Cutáneas Bacterianas/veterinaria , Infección de Heridas/microbiología , Infección de Heridas/patología , Heridas y Lesiones/complicacionesRESUMEN
A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like, Gram-stain-positive bacterium, strain 2710T, isolated from a harbour seal. Comparative 16S rRNA gene sequence analysis showed that this bacterial strain belonged to the genus Arcanobacterium and was related most closely to the type strains of Arcanobacterium phocae (98.4 % similarity) and Arcanobacterium phocisimile (97.5 %). 16S rRNA gene sequence similarities to the type strains of other Arcanobacterium species were between 95.3 and 96.9 %. DNA-DNA hybridization values between strain 2710T and A. phocae DSM 10002T and A. phocisimile LMG 27073T were 4.7 % (reciprocal 56 %) and 23 % (reciprocal 7.7 %), respectively. The presence of the major menaquinone MK-9(H4) and a polar lipid profile with the major compounds diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside supported the affiliation of strain 2710T to the genus Arcanobacterium. The major fatty acids were C16:0, C18:1ω9c, C18:0 and C18:2ω6,9c/anteiso-C18:0. The peptidoglycan structure was of cross-linkage type A5α (l-Lys-l-Lys-d-Glu). Physiological and biochemical tests clearly distinguished the isolate from other members of the genus Arcanobacterium. Based on these tests, it is proposed that this unknown bacterium should be classified as a novel species of the genus Arcanobacterium, with the name Arcanobacterium pinnipediorum sp. nov. The type strain is 2710T ( = DSM 28752T = LMG 28298T).
Asunto(s)
Arcanobacterium/clasificación , Phoca/microbiología , Filogenia , Animales , Arcanobacterium/genética , Arcanobacterium/aislamiento & purificación , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Mar del Norte , Hibridación de Ácido Nucleico , Peptidoglicano/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment.
Asunto(s)
Arcanobacterium/clasificación , Arcanobacterium/aislamiento & purificación , Técnicas de Amplificación de Ácido Nucleico/métodos , Arcanobacterium/genética , ADN Bacteriano/análisis , TemperaturaRESUMEN
Bacteriological examinations were conducted for seven Arcanobacterium haemolyticum strains isolated from elderly patients with skin and soft-tissue infections, such as cellulitis and skin ulcers. Streptococcus dysgalactiae or Gram-positive cocci were isolated together with A. haemolyticum from all patients. The strains were identified as A. haemolyticum based on their being catalase negative, reverse Christie, Atkins and Munch-Petersen (CAMP) positive and phospholipase D gene positive in respective tests. Moreover, API Coryne and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry confirmed the identification of A. haemolyticum. All strains showed good susceptibility to minocycline, vancomycin and ß-lactam antibiotics, but several strains were resistant to gentamicin and levofloxacin.
Asunto(s)
Arcanobacterium/aislamiento & purificación , Enfermedades Cutáneas Bacterianas/microbiología , Infecciones de los Tejidos Blandos/microbiología , Anciano , Anciano de 80 o más Años , Arcanobacterium/química , Arcanobacterium/clasificación , Arcanobacterium/genética , Técnicas de Tipificación Bacteriana , Coinfección/microbiología , Femenino , Genotipo , Cocos Grampositivos/aislamiento & purificación , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Fosfolipasa D/genética , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
A new type of pyoderma was detected in Finnish fur animals in 2007. The disease continues to spread within and between farms, with severe and potentially fatal symptoms. It compromises animal welfare and causes considerable economic losses to farmers. A case-control study was performed in 2010-2011 to describe the entity and to identify the causative agent. Altogether 99 fur animals were necropsied followed by pathological and microbiological examination. The data indicated that the disease clinically manifests in mink (Neovison vison) by necrotic dermatitis of the feet and facial skin. In finnraccoons (Nyctereutes procyonoides), it causes painful abscesses in the paws. Foxes (Vulpes lagopus) are affected by severe conjunctivitis and the infection rapidly spreads to the eyelids and facial skin. A common finding at necropsy was necrotic pyoderma. Microbiological analysis revealed the presence of a number of potential causative agents, including a novel Streptococcus sp. The common finding from all diseased animals of all species was Arcanobacterium phocae. This bacterium has previously been isolated from marine mammals with skin lesions but this is the first report of A. phocae isolated in fur animals with pyoderma. The results obtained from this study implicate A. phocae as a potential causative pathogen of fur animal epidemic necrotic pyoderma (FENP) and support observations that the epidemic may have originated in a species-shift of the causative agent from marine mammals. The variable disease pattern and the presence of other infectious agents (in particular the novel Streptococcus sp.) suggest a multifactorial etiology for FENP, and further studies are needed to determine the environmental, immunological and infectious factors contributing to the disease.
Asunto(s)
Infecciones por Actinomycetales/veterinaria , Enfermedades de los Animales/epidemiología , Enfermedades de los Animales/microbiología , Arcanobacterium , Enfermedades de los Animales/diagnóstico , Animales , Arcanobacterium/clasificación , Arcanobacterium/genética , Necrosis/microbiología , Necrosis/patología , ARN Ribosómico 16S/genéticaRESUMEN
Six Arcanobacterium haemolyticum strains isolated from six patients of two hospitals in Denmark were identified phenotypically, also including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and by genotypic methods. The latter were performed by sequencing 16S rDNA and glyceraldehyde 3-phosphate dehydrogenase encoding gene gap and by amplification of an A. haemolyticum specific region of 16S-23S rDNA intergenic spacer region and 23S rDNA. The six A. haemolyticum strains were further investigated for the presence of seven potential virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, collagen binding protein, neuraminidase A and neuraminidase H which appeared to be present in two (seven virulence genes), two (six virulence genes) and two strains (four virulence genes), respectively. The phenotypic and genotypic properties described in the present study might help to reliably identify and further characterize A. haemolyticum isolated from human patients, a species which seems to be of increasing importance.
Asunto(s)
Infecciones por Actinomycetales/microbiología , Arcanobacterium/clasificación , Arcanobacterium/aislamiento & purificación , Arcanobacterium/genética , Arcanobacterium/fisiología , Técnicas de Tipificación Bacteriana , Análisis por Conglomerados , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Intergénico/química , ADN Intergénico/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Dinamarca , Hospitales , Humanos , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética , ARN Ribosómico 23S/genética , Análisis de Secuencia de ADN , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Factores de Virulencia/genéticaRESUMEN
In the present study 13 Arcanobacterium pluranimalium strains isolated from various animal origin could successfully be identified phenotypically by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and the pluranimaliumlysin encoding gene pla. The detection of mass spectra by MALDI-TOF MS and the novel genotypic approach using gene pla might help to identify A. pluranimalium in future and might elucidate the role this species plays in infections of animals.
Asunto(s)
Infecciones por Actinomycetales/microbiología , Infecciones por Actinomycetales/veterinaria , Arcanobacterium/genética , Proteínas Bacterianas/genética , Animales , Arcanobacterium/clasificación , Arcanobacterium/aislamiento & purificación , Bovinos , Perros , Fenotipo , ARN Ribosómico 16S/genética , Ovinos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
Comparable to previously conducted phenotypical and genotypical investigations characterizing Arcanobacterium canis, a newly described species with the type strain A. canis DSM 25104 isolated from an otitis externa of a dog, four additional A. canis strains isolated from infections of three dogs and one cat could reliably be identified by phenotypic properties, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and by sequencing the genomic targets 16S rDNA, 16S-23S rDNA intergenic spacer region, 23S rDNA, and the genes rpoB and gap. All four A. canis investigated in the present study were isolated from the infected animals together with several other bacterial species indicating that the pathogenic importance of A. canis remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approaches might help to identify A. canis in future and might elucidate the role this species plays in infections of dogs and cats.
Asunto(s)
Infecciones por Actinomycetales/veterinaria , Arcanobacterium/clasificación , Arcanobacterium/genética , Enfermedades de los Gatos/microbiología , Enfermedades de los Perros/microbiología , Infecciones por Actinomycetales/microbiología , Animales , Gatos , ADN Espaciador Ribosómico/genética , Perros , Genotipo , Datos de Secuencia Molecular , Otitis Externa/microbiología , Otitis Externa/veterinaria , Filogenia , ARN Ribosómico 16S/genética , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodosRESUMEN
We are describing a clinical case of bovine mastitis due to Arcanobacterium pluranimalium in a Holstein-Friesian heifer, delivering bloody milk on the left hindquarter. Moreover, we report on the development and evaluation of PCR primers based on the pluranimaliumlysin (pla) gene for the identification of this species. With the primer pair PlaF/PlaR the A. pluranimalium type strain as well as the mastitis isolate 704 revealed a correctly sized amplification product (458 bp), whereas no amplification product was obtained for all non-target strains. The established PCR provides a new and convenient tool for the mastitis diagnostic to differentiate between A. pluranimalium and Trueperella pyogenes.
Asunto(s)
Infecciones por Actinomycetales/veterinaria , Arcanobacterium/aislamiento & purificación , Mastitis Bovina/microbiología , Leche/microbiología , Reacción en Cadena de la Polimerasa/veterinaria , Infecciones por Actinomycetales/tratamiento farmacológico , Infecciones por Actinomycetales/microbiología , Animales , Arcanobacterium/clasificación , Arcanobacterium/genética , Bovinos , ADN Espaciador Ribosómico/química , Femenino , Mastitis Bovina/diagnóstico , Mastitis Bovina/tratamiento farmacológico , Reacción en Cadena de la Polimerasa/métodos , ARN Ribosómico 16S/genética , ARN Ribosómico 23S/genéticaRESUMEN
A polyphasic taxonomic study was performed on two previously unidentified Arcanobacterium-like Gram-positive strains isolated from harbour seals. Comparative 16S rRNA gene sequencing showed that both bacteria belonged to the genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum CIP 103370(T) (98.4% 16S rRNA gene sequence similarity), A. canis P6775(T) (97.4%), A. phocae DSM 10002(T) (97.4%), A. pluranimalium M430/94/2(T) (95.7%) and A. hippocoleae CCUG 44697(T) (95.5%). The presence of the major menaquinone MK-9(H4) supported the affiliation of the isolates with the genus Arcanobacterium. The polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and two unidentified glycolipids. The major fatty acids were C16:0, C18:0, C18:1ω9c and summed feature 5 (comprising C18:2ω6,9c and/or anteiso-C18:0). Physiological and biochemical tests clearly distinguished the isolates from other members of the genus Arcanobacterium. Based on the common origin and various physiological properties comparable to those of A. phocae, it is proposed that the isolates are classified as members of a novel species with the name Arcanobacterium phocisimile sp. nov. The type strain is 2698(T) (=LMG 27073(T) =CCM 8430(T)).
Asunto(s)
Arcanobacterium/clasificación , Phoca/microbiología , Filogenia , Animales , Arcanobacterium/genética , Arcanobacterium/aislamiento & purificación , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Ácidos Grasos/análisis , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Quinonas/análisis , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
In the present study a Trueperella (T.) pyogenes strain isolated from an abscess on the left side of the face of a six year old grey slender loris (Loris lydekkerianus nordicus) could successfully be identified phenotypically, by MALDI-TOF MS analysis and genotypically using T. pyogenes superoxide dismutase A encoding gene sodA and T. pyogenes 16S-23S rDNA intergenic spacer region specific oligonucleotide primers. The T. pyogenes strain could additionally be characterized by PCR-mediated amplification of several known and putative virulence factor encoding genes which revealed the presence of the genes plo encoding pyolysin, nanH encoding neuraminidase NanH and the genes fimA, fimC, fimE encoding the fimbrial subunits FimA, FimC and FimE but not the genes cbpA and nanP encoding collagen-binding protein CbpA and neuraminidase NanP, respectively. The present data give the first information about properties of T. pyogenes isolated from a monkey.
Asunto(s)
Absceso/veterinaria , Infecciones por Actinomycetales/veterinaria , Animales de Zoológico/microbiología , Arcanobacterium/clasificación , Arcanobacterium/aislamiento & purificación , Cara/microbiología , Lorisidae/microbiología , Absceso/diagnóstico , Absceso/microbiología , Infecciones por Actinomycetales/microbiología , Animales , Alemania , Masculino , Fenotipo , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
Arcanobacterium haemolyticum is a rarely reported human pathogen but can cause wound infections in elderly patients with immunodeficiency and pharyngotonsillitis in adolescents and young adults. A. haemolyticum septicaemia originating from a wound rarely occurs and mainly affects immunocompromised patients. Here, we report a case of A. haemolyticum septicaemia in an immunocompetent patient with no underlying diseases.
Asunto(s)
Infecciones por Actinomycetales/microbiología , Arcanobacterium/clasificación , Arcanobacterium/aislamiento & purificación , Inmunocompetencia , Sepsis/microbiología , Infecciones por Actinomycetales/tratamiento farmacológico , Antibacterianos/uso terapéutico , Arcanobacterium/fisiología , Humanos , Masculino , Persona de Mediana Edad , Sepsis/tratamiento farmacológicoRESUMEN
In the present study a Trueperella (Arcanobacterium) bernardiae strain isolated from an anal swab of a three-day-old piglet could be identified phenotypically, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing the 16S rDNA, the 16S-23S rDNA intergenic spacer region (ISR) and by sequencing the superoxide dismutase A encoding gene sodA. The present study gives the first information about the presence of T. (A.) bernardiae in specimen of animals.
Asunto(s)
Arcanobacterium/aislamiento & purificación , Arcanobacterium/fisiología , Enfermedades de los Porcinos/microbiología , Animales , Arcanobacterium/clasificación , Proteínas Bacterianas/genética , ADN Bacteriano/genética , ADN Espaciador Ribosómico/genética , Humanos , Filogenia , ARN Ribosómico 16S/genética , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Superóxido Dismutasa/genética , PorcinosRESUMEN
In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16s-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans.